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The map label for this gene is rlmL

Identifier: 93005602

GI number: 93005602

Start: 908697

End: 911027

Strand: Reverse

Name: rlmL

Synonym: Pcryo_0772

Alternate gene names: 93005602

Gene position: 911027-908697 (Counterclockwise)

Preceding gene: 93005603

Following gene: 93005601

Centisome position: 29.77

GC content: 43.54

Gene sequence:

>2331_bases
ATGACCGAAACAACCGCGCTCGCCTCATCCACAGCGTCTTCGCAACCATCTTCACAAGCATCTTCACAGGTATTGTCGCT
TGACCTTATCATCACCTGCGCCGATGGGTTAGAAGCACCGCTACAGACTGAGCTGACCAGTTTTGGTATTGCAAGTGAGA
TAAAGAGTACCGGTCGCCTTGCTGTTACGGGTACCTTGCGTGATTTATATAAGATCTGTCTTTGGTCGCGCGTGGCATCG
CGCGTATTAATGCTGATTAAACGCAAAAATATCAATGCCGAATATGATGTGGCTGAGCAGCTGTATGGTTTGGCGAAATC
GGTCAATTGGACAGAGCAATTTAGCCTAGAGCAAACCTTTGCGATTCGTCTTTCTGTTGATAAACGTGTGGCTGTCAGTC
AACAGTTTGCCATGCTACGTATTAAAGATGCGATTGCGGATACCTTTAACGAAGTTTATGAGAGCCGCCCTAACGTCGAT
AGTAAAAACCCAGACTTTTCTGTTTTTGCGACCGTCAATGATAAGCAAGCTGAGTTATATCTAGATTTGTCAGGTACTAG
CTTGCATCGCCGTGGCTATCGCGTTGCGATGACAGAAGCACCACTAAAAGAAAACTTAGCAGCGGCGCTACTTTATAGCG
CGGGCTGGCATAAGAAAAACGAAGCAGGTGATACGCCTTTTTATAATGCCTTAATCGATCCTATGTGTGGTTCTGGTACC
TTTATTATTGAAGCGCTCCTCATGCACTGCGATTATGCTGTCGGTATCGATAAAGCCGCCAATCAGTTTGGTTTTTACGA
GTGGCAGCATCATGACGCGGCACTATGGCAAGAAATGATTGATGATGCACAGACCCGTTTCCGTGCAGCATTAGAGATTG
CTAATGAGCAGCCAGATACATTACCGCTTATCTTTGGATTTGATGCCGATAATGGCGCTATCATTGCCACAGAAAAGAAT
CTGATTGCTGCTGGTTTACAAGACCTGTTACCGCTACTCGATATCGAAACGCGCGCCCTTGATCAGCTAAGCACGCTCTT
AAAACCTTTGGTTGCTGACGGTCGTTTGAGCAATCCTTTGATTATCACCAACCCACCTTATGGTGAGCGTCTGGGCGATG
AAGAAATGATTAAGCCGTTGTATCAATCGATTGGGCTTATCTTACAAGACAGCTTTGCCGGTAGTGGTATTGATCCGATG
CTTGGTATATTGGCGTCGCATGTTGAGCAAGTCGATATTTTGCCGATTAGAGAACCAAAAACGTTACGGTGTCATAATGG
TGCCATTACCGTGTATTTCCGCTATGGCACCTTAATTGCTGGTCAGACAGGGAATCTCGTTAGCCGTTTTGAGAAACGTG
AAATTGAAGTTGAAGACGGTCAAGATTTTATCAATCGTTTGCAAAAAAACCTGACCCGACTAAAGAAATTGGCTAAAAAA
GACAACGTGAGTAATATCCGTGTCTACAATGCTGACCTGCCTGATTTCAAAGTGGCAATCGACTTATATGGCGACTATGC
TCATGTACAAGAGTACGCGCCGCCGAAGACCATTCCACCAGAAACCGCCAAAAAACGCTTTAATTTGGCATTAATGGGTA
TTCGTGAAGTATTCGGTATTAACCGCGAACAAATCTTTATTAAAACCCGTGCTCGTCAATCCGGTAATGATCAATATAGC
AAACAAGGCAATACCGAAAAGCGTGGCAAATTTTATATTGCCCGCGAAGATGGTGCTTATCTTTATGTCAATTTCACCGA
TTATCTCGATACGGGTCTATTTATTGACCATCGCAATATGCGTGCGCGTATCAAAGACAACAGTCGTAATAAATCGGTAT
TGAACTTATTCGCTTATACTTGTACGGCGAGTGTGCATGCGGCATTGGCGGGCGCTAAAAAAGTCACCAGCGTTGACTTA
TCACAGAACTATCTGGATTGGGGCAAACAAAACTTTGCTTTAAATGGCTTGAATGTCAGTAGTAATAAATATCAATTCGT
TGCTGCCGATATTTTTGAGTGGATCAAAGACAATACGGAACAATTCGATATTATCTTTATTGATCCGCCAACCTTTTCAA
ACTCTAAAAAGTTTCAAGGTACCTTTGATGTCCAGCGTGACCATGCTGCACTAATCAACCGCGCGATGAACCGCTTAACG
GCAGATGGCATCTTATACTTCTCTAATAACTTTACCCGTTTTGAGTTGGATGAACAGCTGACTGAGCGTTATGACATCAT
CGACATTACCCAAAAAACCATTGGTTTTGATTTTGATATCAAAAAACCGATTCATCAAAGCTTTGAGATTCGTCATCGTC
AGAGTCTATAA

Upstream 100 bases:

>100_bases
GCGCTTTAAACCATCCGCTATAGAATTATGGTAAAATTAGCGGTTAAACATCTTATTTTATGATGTGCACTATTTATCAA
TTTAGCCCACAGGTATCCGT

Downstream 100 bases:

>100_bases
CAAACACTAAATCTTAGCTTTGACGCCTAACTTGCTATAAAACATAACAATGACCCAATCAGCTTTGATTGGGTCATTTT
TTGTTGGTATGATAGAGAGC

Product: 23S rRNA m(2)G2445 methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL

Number of amino acids: Translated: 776; Mature: 775

Protein sequence:

>776_residues
MTETTALASSTASSQPSSQASSQVLSLDLIITCADGLEAPLQTELTSFGIASEIKSTGRLAVTGTLRDLYKICLWSRVAS
RVLMLIKRKNINAEYDVAEQLYGLAKSVNWTEQFSLEQTFAIRLSVDKRVAVSQQFAMLRIKDAIADTFNEVYESRPNVD
SKNPDFSVFATVNDKQAELYLDLSGTSLHRRGYRVAMTEAPLKENLAAALLYSAGWHKKNEAGDTPFYNALIDPMCGSGT
FIIEALLMHCDYAVGIDKAANQFGFYEWQHHDAALWQEMIDDAQTRFRAALEIANEQPDTLPLIFGFDADNGAIIATEKN
LIAAGLQDLLPLLDIETRALDQLSTLLKPLVADGRLSNPLIITNPPYGERLGDEEMIKPLYQSIGLILQDSFAGSGIDPM
LGILASHVEQVDILPIREPKTLRCHNGAITVYFRYGTLIAGQTGNLVSRFEKREIEVEDGQDFINRLQKNLTRLKKLAKK
DNVSNIRVYNADLPDFKVAIDLYGDYAHVQEYAPPKTIPPETAKKRFNLALMGIREVFGINREQIFIKTRARQSGNDQYS
KQGNTEKRGKFYIAREDGAYLYVNFTDYLDTGLFIDHRNMRARIKDNSRNKSVLNLFAYTCTASVHAALAGAKKVTSVDL
SQNYLDWGKQNFALNGLNVSSNKYQFVAADIFEWIKDNTEQFDIIFIDPPTFSNSKKFQGTFDVQRDHAALINRAMNRLT
ADGILYFSNNFTRFELDEQLTERYDIIDITQKTIGFDFDIKKPIHQSFEIRHRQSL

Sequences:

>Translated_776_residues
MTETTALASSTASSQPSSQASSQVLSLDLIITCADGLEAPLQTELTSFGIASEIKSTGRLAVTGTLRDLYKICLWSRVAS
RVLMLIKRKNINAEYDVAEQLYGLAKSVNWTEQFSLEQTFAIRLSVDKRVAVSQQFAMLRIKDAIADTFNEVYESRPNVD
SKNPDFSVFATVNDKQAELYLDLSGTSLHRRGYRVAMTEAPLKENLAAALLYSAGWHKKNEAGDTPFYNALIDPMCGSGT
FIIEALLMHCDYAVGIDKAANQFGFYEWQHHDAALWQEMIDDAQTRFRAALEIANEQPDTLPLIFGFDADNGAIIATEKN
LIAAGLQDLLPLLDIETRALDQLSTLLKPLVADGRLSNPLIITNPPYGERLGDEEMIKPLYQSIGLILQDSFAGSGIDPM
LGILASHVEQVDILPIREPKTLRCHNGAITVYFRYGTLIAGQTGNLVSRFEKREIEVEDGQDFINRLQKNLTRLKKLAKK
DNVSNIRVYNADLPDFKVAIDLYGDYAHVQEYAPPKTIPPETAKKRFNLALMGIREVFGINREQIFIKTRARQSGNDQYS
KQGNTEKRGKFYIAREDGAYLYVNFTDYLDTGLFIDHRNMRARIKDNSRNKSVLNLFAYTCTASVHAALAGAKKVTSVDL
SQNYLDWGKQNFALNGLNVSSNKYQFVAADIFEWIKDNTEQFDIIFIDPPTFSNSKKFQGTFDVQRDHAALINRAMNRLT
ADGILYFSNNFTRFELDEQLTERYDIIDITQKTIGFDFDIKKPIHQSFEIRHRQSL
>Mature_775_residues
TETTALASSTASSQPSSQASSQVLSLDLIITCADGLEAPLQTELTSFGIASEIKSTGRLAVTGTLRDLYKICLWSRVASR
VLMLIKRKNINAEYDVAEQLYGLAKSVNWTEQFSLEQTFAIRLSVDKRVAVSQQFAMLRIKDAIADTFNEVYESRPNVDS
KNPDFSVFATVNDKQAELYLDLSGTSLHRRGYRVAMTEAPLKENLAAALLYSAGWHKKNEAGDTPFYNALIDPMCGSGTF
IIEALLMHCDYAVGIDKAANQFGFYEWQHHDAALWQEMIDDAQTRFRAALEIANEQPDTLPLIFGFDADNGAIIATEKNL
IAAGLQDLLPLLDIETRALDQLSTLLKPLVADGRLSNPLIITNPPYGERLGDEEMIKPLYQSIGLILQDSFAGSGIDPML
GILASHVEQVDILPIREPKTLRCHNGAITVYFRYGTLIAGQTGNLVSRFEKREIEVEDGQDFINRLQKNLTRLKKLAKKD
NVSNIRVYNADLPDFKVAIDLYGDYAHVQEYAPPKTIPPETAKKRFNLALMGIREVFGINREQIFIKTRARQSGNDQYSK
QGNTEKRGKFYIAREDGAYLYVNFTDYLDTGLFIDHRNMRARIKDNSRNKSVLNLFAYTCTASVHAALAGAKKVTSVDLS
QNYLDWGKQNFALNGLNVSSNKYQFVAADIFEWIKDNTEQFDIIFIDPPTFSNSKKFQGTFDVQRDHAALINRAMNRLTA
DGILYFSNNFTRFELDEQLTERYDIIDITQKTIGFDFDIKKPIHQSFEIRHRQSL

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1787181, Length=715, Percent_Identity=37.2027972027972, Blast_Score=461, Evalue=1e-131,
Organism=Escherichia coli, GI145693111, Length=154, Percent_Identity=37.012987012987, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLML_PSYCK (Q1QCP8)

Other databases:

- EMBL:   CP000323
- RefSeq:   YP_580039.1
- ProteinModelPortal:   Q1QCP8
- SMR:   Q1QCP8
- STRING:   Q1QCP8
- GeneID:   4034006
- GenomeReviews:   CP000323_GR
- KEGG:   pcr:Pcryo_0772
- NMPDR:   fig|335284.3.peg.135
- eggNOG:   COG0116
- HOGENOM:   HBG289536
- OMA:   YRIYDAD
- PhylomeDB:   Q1QCP8
- ProtClustDB:   PRK11783
- BioCyc:   PCRY335284:PCRYO_0772-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01858
- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114
- PIRSF:   PIRSF037618
- SMART:   SM00981

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020

EC number: =2.1.1.173

Molecular weight: Translated: 87394; Mature: 87263

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS51165 THUMP; PS01261 UPF0020; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETTALASSTASSQPSSQASSQVLSLDLIITCADGLEAPLQTELTSFGIASEIKSTGRL
CCCCHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCCCHHHHHHHHCHHHHHHCCCCE
AVTGTLRDLYKICLWSRVASRVLMLIKRKNINAEYDVAEQLYGLAKSVNWTEQFSLEQTF
EEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEE
AIRLSVDKRVAVSQQFAMLRIKDAIADTFNEVYESRPNVDSKNPDFSVFATVNDKQAELY
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEE
LDLSGTSLHRRGYRVAMTEAPLKENLAAALLYSAGWHKKNEAGDTPFYNALIDPMCGSGT
EEECCCCHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCH
FIIEALLMHCDYAVGIDKAANQFGFYEWQHHDAALWQEMIDDAQTRFRAALEIANEQPDT
HHHHHHHHHCCHHCCCHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LPLIFGFDADNGAIIATEKNLIAAGLQDLLPLLDIETRALDQLSTLLKPLVADGRLSNPL
EEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCE
IITNPPYGERLGDEEMIKPLYQSIGLILQDSFAGSGIDPMLGILASHVEQVDILPIREPK
EEECCCCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHEEEEECCCCC
TLRCHNGAITVYFRYGTLIAGQTGNLVSRFEKREIEVEDGQDFINRLQKNLTRLKKLAKK
EEEEECCEEEEEEEECEEEECCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHH
DNVSNIRVYNADLPDFKVAIDLYGDYAHVQEYAPPKTIPPETAKKRFNLALMGIREVFGI
CCCCEEEEEECCCCCEEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHCCCEEEHHHHHCC
NREQIFIKTRARQSGNDQYSKQGNTEKRGKFYIAREDGAYLYVNFTDYLDTGLFIDHRNM
CCCEEEEEECCCCCCCCHHHHCCCCCCCCEEEEEECCCCEEEEEEHHHHCCCEEEECCCC
RARIKDNSRNKSVLNLFAYTCTASVHAALAGAKKVTSVDLSQNYLDWGKQNFALNGLNVS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHHHHCCCCCEEEECEECC
SNKYQFVAADIFEWIKDNTEQFDIIFIDPPTFSNSKKFQGTFDVQRDHAALINRAMNRLT
CCCEEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHC
ADGILYFSNNFTRFELDEQLTERYDIIDITQKTIGFDFDIKKPIHQSFEIRHRQSL
CCCEEEEECCCEEEEHHHHHHHHCCEEEEHHHHHCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TETTALASSTASSQPSSQASSQVLSLDLIITCADGLEAPLQTELTSFGIASEIKSTGRL
CCCHHHHCCCCCCCCCHHHHHHEEEEEEEEEECCCCCCCHHHHHHHHCHHHHHHCCCCE
AVTGTLRDLYKICLWSRVASRVLMLIKRKNINAEYDVAEQLYGLAKSVNWTEQFSLEQTF
EEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHHHCCCCEEE
AIRLSVDKRVAVSQQFAMLRIKDAIADTFNEVYESRPNVDSKNPDFSVFATVNDKQAELY
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCEEEE
LDLSGTSLHRRGYRVAMTEAPLKENLAAALLYSAGWHKKNEAGDTPFYNALIDPMCGSGT
EEECCCCHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCH
FIIEALLMHCDYAVGIDKAANQFGFYEWQHHDAALWQEMIDDAQTRFRAALEIANEQPDT
HHHHHHHHHCCHHCCCHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LPLIFGFDADNGAIIATEKNLIAAGLQDLLPLLDIETRALDQLSTLLKPLVADGRLSNPL
EEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCE
IITNPPYGERLGDEEMIKPLYQSIGLILQDSFAGSGIDPMLGILASHVEQVDILPIREPK
EEECCCCCCCCCHHHHHHHHHHHHCEEEEECCCCCCCCHHHHHHHHHHHHEEEEECCCCC
TLRCHNGAITVYFRYGTLIAGQTGNLVSRFEKREIEVEDGQDFINRLQKNLTRLKKLAKK
EEEEECCEEEEEEEECEEEECCCCHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHHHH
DNVSNIRVYNADLPDFKVAIDLYGDYAHVQEYAPPKTIPPETAKKRFNLALMGIREVFGI
CCCCEEEEEECCCCCEEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHCCCEEEHHHHHCC
NREQIFIKTRARQSGNDQYSKQGNTEKRGKFYIAREDGAYLYVNFTDYLDTGLFIDHRNM
CCCEEEEEECCCCCCCCHHHHCCCCCCCCEEEEEECCCCEEEEEEHHHHCCCEEEECCCC
RARIKDNSRNKSVLNLFAYTCTASVHAALAGAKKVTSVDLSQNYLDWGKQNFALNGLNVS
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCHHHHHCCCCCEEEECEECC
SNKYQFVAADIFEWIKDNTEQFDIIFIDPPTFSNSKKFQGTFDVQRDHAALINRAMNRLT
CCCEEEHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEECCCHHHHHHHHHHHHHHC
ADGILYFSNNFTRFELDEQLTERYDIIDITQKTIGFDFDIKKPIHQSFEIRHRQSL
CCCEEEEECCCEEEEHHHHHHHHCCEEEEHHHHHCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA