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The map label for this gene is lipA [H]

Identifier: 93005574

GI number: 93005574

Start: 875368

End: 876411

Strand: Reverse

Name: lipA [H]

Synonym: Pcryo_0744

Alternate gene names: 93005574

Gene position: 876411-875368 (Counterclockwise)

Preceding gene: 93005575

Following gene: 93005570

Centisome position: 28.64

GC content: 45.21

Gene sequence:

>1044_bases
ATGACAAACGCCGTACAAACGCACACGCCTGCTGCCCGCGCAAAACCCAAAAAAGCCATCCAAGGCGAAAAACTCCGTGG
CTACGATAAAGTTGCTCGTATCCCAATTAAAATTATCCCGACTGTCGAGGCTAAGAAAAAGCCAGATTGGATTCGGGTAA
AACTGTCTTCACCTGCGGAAGTCGCACGTATCAAGTCCACCTTACGTGAACAGAAACTCTATACCGTTTGTGAAGAAGCT
GCCTGCCCTAACCTTCCACAGTGCTTTGCCGATGGTACTGCTACCTTTATGATTATGGGTGATATCTGTACACGTCGATG
CCCGTTCTGTGATGTCGGACATGGTCGTCCAAATGAGCTTGATAAAGATGAGCCGCGTCATACTGCCGAGACCATCCAAG
GTTTAGGTCTTAAATATGCCGTCATTACCTCGGTTGACCGTGATGATTTAAAAGATGGCGGCGCGATGCATTTCGTTGAA
GTATTGAATGAATCGCGTGCGCTGAGCCCAAATTGCTTGATCGAGATTTTGGTACCAGATTTCCGTGGTCGTATGGATAT
CGCATTGGACTTATTGACTGAAACGGCGCCTGATGTTTTTAACCACAATATCGAAACCGTGCCTCGTTTATATAAAGCTT
TCCGTCCAGGTTCAGATTATCAGCATTCATTAGACTTGCTAAAGATTTATAAAGAACGTCGCCCTGATATTGCGACCAAA
TGCGGCTTTATGGTTGGACTTGGTGAAACTGAAGAAGAAATCTATAAGCTGCTTGATGATCTAAAGGCGCATAACGTCGA
TATGATTACCGTTGGTCAGTATTTACAACCTAGTAAAGACCATGCGCCAGTCGATCGCTATGTGCATCCAGATGAGTTCC
AGCGTTATATGGACTATGGCAAAAAGATTGGCTTCTTTAATATTTGGGCAGGTCCTATGGTGCGCTCAAGCTATTTTGCT
GATCGTCAGTACTATGGTGAAGATTGCCCTGCACCTATCCGTAGCAAAAAAGCATTAGCAGCAGAAGGGAAACTTGGCTG
TTAA

Upstream 100 bases:

>100_bases
AAAACCTTGACCAAATATCTGCTATAATAGCTCTATTCATAAAGCATTCTAATAATTCCATAAACCCTTGTTCTATCAAG
GGTTAAGAGAACATTCGATT

Downstream 100 bases:

>100_bases
GTGAGAGTCGTCACTATATCAACGTAAAATGACGAGTTTTAAAATATAAAAAGGTCGAATAGCAGATATCTACTATTCGA
CCTTTTCTATAGTAAATTCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA [H]

Number of amino acids: Translated: 347; Mature: 346

Protein sequence:

>347_residues
MTNAVQTHTPAARAKPKKAIQGEKLRGYDKVARIPIKIIPTVEAKKKPDWIRVKLSSPAEVARIKSTLREQKLYTVCEEA
ACPNLPQCFADGTATFMIMGDICTRRCPFCDVGHGRPNELDKDEPRHTAETIQGLGLKYAVITSVDRDDLKDGGAMHFVE
VLNESRALSPNCLIEILVPDFRGRMDIALDLLTETAPDVFNHNIETVPRLYKAFRPGSDYQHSLDLLKIYKERRPDIATK
CGFMVGLGETEEEIYKLLDDLKAHNVDMITVGQYLQPSKDHAPVDRYVHPDEFQRYMDYGKKIGFFNIWAGPMVRSSYFA
DRQYYGEDCPAPIRSKKALAAEGKLGC

Sequences:

>Translated_347_residues
MTNAVQTHTPAARAKPKKAIQGEKLRGYDKVARIPIKIIPTVEAKKKPDWIRVKLSSPAEVARIKSTLREQKLYTVCEEA
ACPNLPQCFADGTATFMIMGDICTRRCPFCDVGHGRPNELDKDEPRHTAETIQGLGLKYAVITSVDRDDLKDGGAMHFVE
VLNESRALSPNCLIEILVPDFRGRMDIALDLLTETAPDVFNHNIETVPRLYKAFRPGSDYQHSLDLLKIYKERRPDIATK
CGFMVGLGETEEEIYKLLDDLKAHNVDMITVGQYLQPSKDHAPVDRYVHPDEFQRYMDYGKKIGFFNIWAGPMVRSSYFA
DRQYYGEDCPAPIRSKKALAAEGKLGC
>Mature_346_residues
TNAVQTHTPAARAKPKKAIQGEKLRGYDKVARIPIKIIPTVEAKKKPDWIRVKLSSPAEVARIKSTLREQKLYTVCEEAA
CPNLPQCFADGTATFMIMGDICTRRCPFCDVGHGRPNELDKDEPRHTAETIQGLGLKYAVITSVDRDDLKDGGAMHFVEV
LNESRALSPNCLIEILVPDFRGRMDIALDLLTETAPDVFNHNIETVPRLYKAFRPGSDYQHSLDLLKIYKERRPDIATKC
GFMVGLGETEEEIYKLLDDLKAHNVDMITVGQYLQPSKDHAPVDRYVHPDEFQRYMDYGKKIGFFNIWAGPMVRSSYFAD
RQYYGEDCPAPIRSKKALAAEGKLGC

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=280, Percent_Identity=42.1428571428571, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI37577164, Length=250, Percent_Identity=42, Blast_Score=207, Evalue=1e-53,
Organism=Escherichia coli, GI1786846, Length=318, Percent_Identity=55.6603773584906, Blast_Score=357, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI32564533, Length=287, Percent_Identity=45.2961672473868, Blast_Score=237, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6324770, Length=291, Percent_Identity=41.5807560137457, Blast_Score=232, Evalue=5e-62,
Organism=Drosophila melanogaster, GI221513272, Length=280, Percent_Identity=44.6428571428571, Blast_Score=243, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 39139; Mature: 39008

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAVQTHTPAARAKPKKAIQGEKLRGYDKVARIPIKIIPTVEAKKKPDWIRVKLSSPAE
CCCCCCCCCCHHHCCCHHHHCCCHHCCHHHHHHCCEEEEECCCCCCCCCEEEEEECCCHH
VARIKSTLREQKLYTVCEEAACPNLPQCFADGTATFMIMGDICTRRCPFCDVGHGRPNEL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCC
DKDEPRHTAETIQGLGLKYAVITSVDRDDLKDGGAMHFVEVLNESRALSPNCLIEILVPD
CCCCCHHHHHHHHHCCCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCC
FRGRMDIALDLLTETAPDVFNHNIETVPRLYKAFRPGSDYQHSLDLLKIYKERRPDIATK
CCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHH
CGFMVGLGETEEEIYKLLDDLKAHNVDMITVGQYLQPSKDHAPVDRYVHPDEFQRYMDYG
CCEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KKIGFFNIWAGPMVRSSYFADRQYYGEDCPAPIRSKKALAAEGKLGC
HCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
TNAVQTHTPAARAKPKKAIQGEKLRGYDKVARIPIKIIPTVEAKKKPDWIRVKLSSPAE
CCCCCCCCCHHHCCCHHHHCCCHHCCHHHHHHCCEEEEECCCCCCCCCEEEEEECCCHH
VARIKSTLREQKLYTVCEEAACPNLPQCFADGTATFMIMGDICTRRCPFCDVGHGRPNEL
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEEEEHHHHCCCCCCCCCCCCCCCC
DKDEPRHTAETIQGLGLKYAVITSVDRDDLKDGGAMHFVEVLNESRALSPNCLIEILVPD
CCCCCHHHHHHHHHCCCEEEEEECCCHHHHCCCCHHHHHHHHHHCCCCCCCCEEEEECCC
FRGRMDIALDLLTETAPDVFNHNIETVPRLYKAFRPGSDYQHSLDLLKIYKERRPDIATK
CCCCHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHH
CGFMVGLGETEEEIYKLLDDLKAHNVDMITVGQYLQPSKDHAPVDRYVHPDEFQRYMDYG
CCEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
KKIGFFNIWAGPMVRSSYFADRQYYGEDCPAPIRSKKALAAEGKLGC
HCCCEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA