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The map label for this gene is rep [H]

Identifier: 93005402

GI number: 93005402

Start: 680735

End: 682768

Strand: Reverse

Name: rep [H]

Synonym: Pcryo_0572

Alternate gene names: 93005402

Gene position: 682768-680735 (Counterclockwise)

Preceding gene: 93005417

Following gene: 93005401

Centisome position: 22.31

GC content: 44.59

Gene sequence:

>2034_bases
ATGAGTCAACTTAATCCCAAACAACAAGAAGCCATGCTCTACGTATCTGGTCCGCTACTGGTGTTAGCAGGTGCCGGCTC
CGGTAAGACGTCAGTGATTACCCGCAAGATTGCTTATTTGATCGAAGAATGCAACATGCCTGCTGAGCGTATCACGGCGG
TGACCTTTACTAATAAAGCGGCGCGTGAAATGAAAGCTCGAGTCAGTAAGCTACTACCGAGTGAAAAGACCCGTGGTCTA
ACGGTTTCAACCTTTCACCAATTTGGGCTCCAGTTTCTGCGATATGAGCTGCTTCATACGCCATTGAAAGGCAATTTCTC
CATTATGGACAGCGACGATAGCAAGCGTTTGCTAATGGAGCTTATGATGCGTGATAACTTAAGCGGCGCTGAGAGTCGTG
AGTTGGTTGGTAAAGCAATCAAGATGATTTCTGATTGGAAAAATGATTTGATTGAGCCGGATAAGGCGATGGAAACCTTA
GACGATCCAGAAGATATGATTTTTGCGACGCTTTATGCACTATATGAGCGTAACCTGCGGGCTTATAACGCGGTTGATTT
TGATGATTTGATTGTCTTACCGACCAAAATACTGCGTGAAAATCGAGAGCTACGTGATAAATGGCAAAACCGTATTCGCT
ATTTGCTGGTCGATGAATATCAAGACACCAATACCGCTCAGTATGAGATGATCAAATATTTGGTCGGACCGCAAGGGCGT
TTTACCGTCGTTGGTGATGATGACCAATCTATTTATGCATGGCGCGGTGCTAAACCTGAAAACATGGGATTACTTAAAGA
GGATTTCCCAAAATTAAAAATCGTTATGCTTGAGCAAAACTATCGCTCAACCACGCGTATTTTGACCTCTGCTAATGCGG
TTATTACCAACAATGAGCATTTATTTGAAAAGAAACTCTGGTCAGATAAAGGTCAAGGCGAAAAAATTCGCATTATTAAC
TGTCGTAATGATGACGATGAATCTGAGCGCGTCGCCAAAGAAATCGTCACGCATAAATTGCGCTTTGGTAATGAGTGGGA
ACAATATGCCGTTCTTTATCGCAGTAACTTCCAAGCGCGGATGCTAGAGGCGCAGTTGCGTCAACTGCAAGTGCCTTATA
AATTATCGGGTGGTCAGTCGTTCTTTGCCCGTAGTGAAATTAAAGACGTTATGAGCTACTTACGTCTGATTTTAAACCCA
GAAGACGACAGTGCATTTTTGCGCGTGATTAATACGCCCAAGCGTGGCATGGGACCAGCCACGCTTGAAAAGCTTGGCTT
ATTTTCACAAGAACATAGTATCTCGCTACTGGCTTCTTGTACGCATGCTGGTCTTGCCCACGTTTTACCGTCGAAATCTT
ATAGTACTTTAAAGGAGTTTGGCGACTTTATTGAGCACTATACTCGCGAGCTTGATCAACATCCTGACCCTGTGCCAATC
GTACGCCAGATGATTGATGAAACCGGCTATATTGACTTTGTCCGTAGCGACTCAAAAACGCCGCAACAAGAAAAAAACCG
TATCGATAATATCGACATGCTTTATACCAGTATTCAATCGCTGATTAATCGCGCCGAAGAAGACGAAGACCGTACCATTG
ATACCATCATTCGCAAGCTGGTATTGCTTGATATGCTGGAGCAGCAGCAGGAAGAAGAAAACACCAATAAAGTTAATCTA
ATGACCTTACATGCGGCAAAAGGCTTGGAGTTTGATTTTGTTTATATCATGGGGCTTGAGGAAGAGATGCTACCACATCG
CAACTCTATCCTAAGCGAGACGGTCGAAGAGGAACGGCGCTTGATGTATGTGGGCATTACTCGCGCGCGTCGCGAGCTAA
CTTTGACACTAGCCACTCAGCGCCGTGCTGGTGGTCAAATGCGCGTCACGTCTGAATCACGATTTTTAGATGAGTTGCCA
GAAGATCATATCGATTGGCCTGCCAAAGCAAAAAAACAAAAAGCCACCAAAGACCCCGCAGCAGTTGCTGATGAATACTT
GGCTAATATTCGGGCTTTATTGGGCAATCGATAA

Upstream 100 bases:

>100_bases
GCCCTCTATTTTAACGAATATATTTTTCGCTTGATGGCAGCTAGTTGCATTTTACGATTTACTGCTACCCTCTTTTTGCT
TTTGTCTTACAGGTCACGTT

Downstream 100 bases:

>100_bases
TGTTTAACCATTAAAAAAGCTAACAAATACAACTGATTCATTAAGCTTAGCTCTTAGTCATTTACATATTTTGAAAACTT
GGAACTTTTATGCCCGCTCC

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 677; Mature: 676

Protein sequence:

>677_residues
MSQLNPKQQEAMLYVSGPLLVLAGAGSGKTSVITRKIAYLIEECNMPAERITAVTFTNKAAREMKARVSKLLPSEKTRGL
TVSTFHQFGLQFLRYELLHTPLKGNFSIMDSDDSKRLLMELMMRDNLSGAESRELVGKAIKMISDWKNDLIEPDKAMETL
DDPEDMIFATLYALYERNLRAYNAVDFDDLIVLPTKILRENRELRDKWQNRIRYLLVDEYQDTNTAQYEMIKYLVGPQGR
FTVVGDDDQSIYAWRGAKPENMGLLKEDFPKLKIVMLEQNYRSTTRILTSANAVITNNEHLFEKKLWSDKGQGEKIRIIN
CRNDDDESERVAKEIVTHKLRFGNEWEQYAVLYRSNFQARMLEAQLRQLQVPYKLSGGQSFFARSEIKDVMSYLRLILNP
EDDSAFLRVINTPKRGMGPATLEKLGLFSQEHSISLLASCTHAGLAHVLPSKSYSTLKEFGDFIEHYTRELDQHPDPVPI
VRQMIDETGYIDFVRSDSKTPQQEKNRIDNIDMLYTSIQSLINRAEEDEDRTIDTIIRKLVLLDMLEQQQEEENTNKVNL
MTLHAAKGLEFDFVYIMGLEEEMLPHRNSILSETVEEERRLMYVGITRARRELTLTLATQRRAGGQMRVTSESRFLDELP
EDHIDWPAKAKKQKATKDPAAVADEYLANIRALLGNR

Sequences:

>Translated_677_residues
MSQLNPKQQEAMLYVSGPLLVLAGAGSGKTSVITRKIAYLIEECNMPAERITAVTFTNKAAREMKARVSKLLPSEKTRGL
TVSTFHQFGLQFLRYELLHTPLKGNFSIMDSDDSKRLLMELMMRDNLSGAESRELVGKAIKMISDWKNDLIEPDKAMETL
DDPEDMIFATLYALYERNLRAYNAVDFDDLIVLPTKILRENRELRDKWQNRIRYLLVDEYQDTNTAQYEMIKYLVGPQGR
FTVVGDDDQSIYAWRGAKPENMGLLKEDFPKLKIVMLEQNYRSTTRILTSANAVITNNEHLFEKKLWSDKGQGEKIRIIN
CRNDDDESERVAKEIVTHKLRFGNEWEQYAVLYRSNFQARMLEAQLRQLQVPYKLSGGQSFFARSEIKDVMSYLRLILNP
EDDSAFLRVINTPKRGMGPATLEKLGLFSQEHSISLLASCTHAGLAHVLPSKSYSTLKEFGDFIEHYTRELDQHPDPVPI
VRQMIDETGYIDFVRSDSKTPQQEKNRIDNIDMLYTSIQSLINRAEEDEDRTIDTIIRKLVLLDMLEQQQEEENTNKVNL
MTLHAAKGLEFDFVYIMGLEEEMLPHRNSILSETVEEERRLMYVGITRARRELTLTLATQRRAGGQMRVTSESRFLDELP
EDHIDWPAKAKKQKATKDPAAVADEYLANIRALLGNR
>Mature_676_residues
SQLNPKQQEAMLYVSGPLLVLAGAGSGKTSVITRKIAYLIEECNMPAERITAVTFTNKAAREMKARVSKLLPSEKTRGLT
VSTFHQFGLQFLRYELLHTPLKGNFSIMDSDDSKRLLMELMMRDNLSGAESRELVGKAIKMISDWKNDLIEPDKAMETLD
DPEDMIFATLYALYERNLRAYNAVDFDDLIVLPTKILRENRELRDKWQNRIRYLLVDEYQDTNTAQYEMIKYLVGPQGRF
TVVGDDDQSIYAWRGAKPENMGLLKEDFPKLKIVMLEQNYRSTTRILTSANAVITNNEHLFEKKLWSDKGQGEKIRIINC
RNDDDESERVAKEIVTHKLRFGNEWEQYAVLYRSNFQARMLEAQLRQLQVPYKLSGGQSFFARSEIKDVMSYLRLILNPE
DDSAFLRVINTPKRGMGPATLEKLGLFSQEHSISLLASCTHAGLAHVLPSKSYSTLKEFGDFIEHYTRELDQHPDPVPIV
RQMIDETGYIDFVRSDSKTPQQEKNRIDNIDMLYTSIQSLINRAEEDEDRTIDTIIRKLVLLDMLEQQQEEENTNKVNLM
TLHAAKGLEFDFVYIMGLEEEMLPHRNSILSETVEEERRLMYVGITRARRELTLTLATQRRAGGQMRVTSESRFLDELPE
DHIDWPAKAKKQKATKDPAAVADEYLANIRALLGNR

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=678, Percent_Identity=46.6076696165192, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=648, Percent_Identity=39.0432098765432, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI1787196, Length=352, Percent_Identity=27.8409090909091, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=762, Percent_Identity=26.7716535433071, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324477, Length=677, Percent_Identity=21.7134416543575, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78008; Mature: 77877

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLNPKQQEAMLYVSGPLLVLAGAGSGKTSVITRKIAYLIEECNMPAERITAVTFTNKA
CCCCCCCHHHEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCHH
AREMKARVSKLLPSEKTRGLTVSTFHQFGLQFLRYELLHTPLKGNFSIMDSDDSKRLLME
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
LMMRDNLSGAESRELVGKAIKMISDWKNDLIEPDKAMETLDDPEDMIFATLYALYERNLR
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCC
AYNAVDFDDLIVLPTKILRENRELRDKWQNRIRYLLVDEYQDTNTAQYEMIKYLVGPQGR
CCCCCCCCCHHCCCHHHHHCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCC
FTVVGDDDQSIYAWRGAKPENMGLLKEDFPKLKIVMLEQNYRSTTRILTSANAVITNNEH
EEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEEECCCCHHHHHHHCCCEEEECCHH
LFEKKLWSDKGQGEKIRIINCRNDDDESERVAKEIVTHKLRFGNEWEQYAVLYRSNFQAR
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
MLEAQLRQLQVPYKLSGGQSFFARSEIKDVMSYLRLILNPEDDSAFLRVINTPKRGMGPA
HHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCHH
TLEKLGLFSQEHSISLLASCTHAGLAHVLPSKSYSTLKEFGDFIEHYTRELDQHPDPVPI
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
VRQMIDETGYIDFVRSDSKTPQQEKNRIDNIDMLYTSIQSLINRAEEDEDRTIDTIIRKL
HHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VLLDMLEQQQEEENTNKVNLMTLHAAKGLEFDFVYIMGLEEEMLPHRNSILSETVEEERR
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHH
LMYVGITRARRELTLTLATQRRAGGQMRVTSESRFLDELPEDHIDWPAKAKKQKATKDPA
EEEEEEHHCCCEEEEEEEEHHCCCCCEEECCHHHHHHHCCHHHCCCCCHHHHHHCCCCHH
AVADEYLANIRALLGNR
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQLNPKQQEAMLYVSGPLLVLAGAGSGKTSVITRKIAYLIEECNMPAERITAVTFTNKA
CCCCCCHHHEEEEEECCEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEECCHH
AREMKARVSKLLPSEKTRGLTVSTFHQFGLQFLRYELLHTPLKGNFSIMDSDDSKRLLME
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHH
LMMRDNLSGAESRELVGKAIKMISDWKNDLIEPDKAMETLDDPEDMIFATLYALYERNLR
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCC
AYNAVDFDDLIVLPTKILRENRELRDKWQNRIRYLLVDEYQDTNTAQYEMIKYLVGPQGR
CCCCCCCCCHHCCCHHHHHCCHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCC
FTVVGDDDQSIYAWRGAKPENMGLLKEDFPKLKIVMLEQNYRSTTRILTSANAVITNNEH
EEEECCCCCCEEEECCCCCCCCCCHHHCCCCEEEEEEECCCCHHHHHHHCCCEEEECCHH
LFEKKLWSDKGQGEKIRIINCRNDDDESERVAKEIVTHKLRFGNEWEQYAVLYRSNFQAR
HHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHH
MLEAQLRQLQVPYKLSGGQSFFARSEIKDVMSYLRLILNPEDDSAFLRVINTPKRGMGPA
HHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCCCCCCCHH
TLEKLGLFSQEHSISLLASCTHAGLAHVLPSKSYSTLKEFGDFIEHYTRELDQHPDPVPI
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHH
VRQMIDETGYIDFVRSDSKTPQQEKNRIDNIDMLYTSIQSLINRAEEDEDRTIDTIIRKL
HHHHHHCCCCEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VLLDMLEQQQEEENTNKVNLMTLHAAKGLEFDFVYIMGLEEEMLPHRNSILSETVEEERR
HHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEEECCCHHHCCCHHHHHHHHHHHHHH
LMYVGITRARRELTLTLATQRRAGGQMRVTSESRFLDELPEDHIDWPAKAKKQKATKDPA
EEEEEEHHCCCEEEEEEEEHHCCCCCEEECCHHHHHHHCCHHHCCCCCHHHHHHCCCCHH
AVADEYLANIRALLGNR
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]