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The map label for this gene is prs [H]

Identifier: 93005016

GI number: 93005016

Start: 215617

End: 216564

Strand: Reverse

Name: prs [H]

Synonym: Pcryo_0185

Alternate gene names: 93005016

Gene position: 216564-215617 (Counterclockwise)

Preceding gene: 93005017

Following gene: 93005015

Centisome position: 7.08

GC content: 47.26

Gene sequence:

>948_bases
ATGCCTTATTTGGCGATTTTTACGGGCAATGCCCACCCAGAATTAGCAAAAACCGTAGCCGATCATCTCCATATACCTCT
TGGTAAAGCTGACATCACGCGTTTTTCTGATGGCGAAATTGCCGTAGAAATCAAAGAGCACGTGCGTGGTAAAGACGTGT
TTATCATGCAGCCTACTTGTGCACCGACCAACGACAATTTGATGGAAATCATGATGATGGCAGACGCTTTGCGTCGCTCT
AGTGCTGGTCGTATTACAGCAGTCATGCCGTATTTTGGTTATGCCCGTCAAGACCGTCGCCCTCGTTCAGCTCGTGTGCC
TATCTCTGCTAAAGTCGTTGCTGACATGTTGAACATCGTTAGTATCGATCGTGTGATGACCGTTGATTTGCACTCAGATC
AGATTCAAGGTTTCTTTGACATCCCAGTCGATAATATCTACGGCACGCCTGTATTGCTTAACGATCTACAAAAACAAGAT
TATGACAACATCATGGTTGTCTCTCCTGACGTTGGGGGCGTGGTTCGTGCACGTGCGATGGCTAAGCAGTTGGGCGATAC
TGACATGGCGATTATTGATAAACGTCGTGCCCGTGCCAATGAGTCGCAAGTGATGCACATTATTGGTGACGTCCGTGACC
GTGATTGTGTCATCGTTGATGATATGGTCGATACGGCTGGCACGCTATGTAAAGCTGCCGAAGCGCTTAAAGCAAATGGC
GCACGCCGCGTAGTGGCTTATATCACCCACCCTGTCCTATCTGGTAATGCGCTTAAGAACATCAGTGAATCAGAATTGGA
TGAGATTGTCGTTACTGATACGATTCCATTGTCTGATGCTGCCAAAGCTTGCAGCAAGATTCGTCAAGTGAGCATCGCGC
CAATGTTGGCTGAAAGCTTACGCCGTATTAATAACGAAGAATCTATCAGCGCCATGTTTGACGCTTAA

Upstream 100 bases:

>100_bases
TAATAGTAGTCATCAATCTTTTTCACCACTCTTTTCTGAATTTGAGCAGTTTGACACTGCTTAGCGGTCGCTGCTTGAGT
CAATCAATAGGACTTCTGTC

Downstream 100 bases:

>100_bases
GAGTTATCGTAGATTGAAAAAAGCGCTAAGTTTCATTACTTGGCGCTTTTTTTATTTAATGATTTTAATTAACAGTTTTG
CACTGTTGTTCGTATAGGTG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MPYLAIFTGNAHPELAKTVADHLHIPLGKADITRFSDGEIAVEIKEHVRGKDVFIMQPTCAPTNDNLMEIMMMADALRRS
SAGRITAVMPYFGYARQDRRPRSARVPISAKVVADMLNIVSIDRVMTVDLHSDQIQGFFDIPVDNIYGTPVLLNDLQKQD
YDNIMVVSPDVGGVVRARAMAKQLGDTDMAIIDKRRARANESQVMHIIGDVRDRDCVIVDDMVDTAGTLCKAAEALKANG
ARRVVAYITHPVLSGNALKNISESELDEIVVTDTIPLSDAAKACSKIRQVSIAPMLAESLRRINNEESISAMFDA

Sequences:

>Translated_315_residues
MPYLAIFTGNAHPELAKTVADHLHIPLGKADITRFSDGEIAVEIKEHVRGKDVFIMQPTCAPTNDNLMEIMMMADALRRS
SAGRITAVMPYFGYARQDRRPRSARVPISAKVVADMLNIVSIDRVMTVDLHSDQIQGFFDIPVDNIYGTPVLLNDLQKQD
YDNIMVVSPDVGGVVRARAMAKQLGDTDMAIIDKRRARANESQVMHIIGDVRDRDCVIVDDMVDTAGTLCKAAEALKANG
ARRVVAYITHPVLSGNALKNISESELDEIVVTDTIPLSDAAKACSKIRQVSIAPMLAESLRRINNEESISAMFDA
>Mature_314_residues
PYLAIFTGNAHPELAKTVADHLHIPLGKADITRFSDGEIAVEIKEHVRGKDVFIMQPTCAPTNDNLMEIMMMADALRRSS
AGRITAVMPYFGYARQDRRPRSARVPISAKVVADMLNIVSIDRVMTVDLHSDQIQGFFDIPVDNIYGTPVLLNDLQKQDY
DNIMVVSPDVGGVVRARAMAKQLGDTDMAIIDKRRARANESQVMHIIGDVRDRDCVIVDDMVDTAGTLCKAAEALKANGA
RRVVAYITHPVLSGNALKNISESELDEIVVTDTIPLSDAAKACSKIRQVSIAPMLAESLRRINNEESISAMFDA

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=315, Percent_Identity=47.6190476190476, Blast_Score=308, Evalue=6e-84,
Organism=Homo sapiens, GI4506129, Length=315, Percent_Identity=46.3492063492063, Blast_Score=305, Evalue=3e-83,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=45.7413249211356, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI28557709, Length=315, Percent_Identity=47.3015873015873, Blast_Score=302, Evalue=2e-82,
Organism=Homo sapiens, GI4506133, Length=345, Percent_Identity=34.4927536231884, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI194018537, Length=342, Percent_Identity=35.0877192982456, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI310128524, Length=148, Percent_Identity=28.3783783783784, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI310115209, Length=148, Percent_Identity=28.3783783783784, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI310118259, Length=148, Percent_Identity=28.3783783783784, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI310119946, Length=148, Percent_Identity=28.3783783783784, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1787458, Length=314, Percent_Identity=67.8343949044586, Blast_Score=454, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI25149168, Length=315, Percent_Identity=43.8095238095238, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17554702, Length=315, Percent_Identity=43.8095238095238, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71989924, Length=315, Percent_Identity=43.8095238095238, Blast_Score=286, Evalue=8e-78,
Organism=Caenorhabditis elegans, GI17554704, Length=310, Percent_Identity=43.8709677419355, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=34.0236686390533, Blast_Score=198, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321776, Length=312, Percent_Identity=45.8333333333333, Blast_Score=276, Evalue=3e-75,
Organism=Saccharomyces cerevisiae, GI6319403, Length=315, Percent_Identity=43.8095238095238, Blast_Score=266, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6320946, Length=313, Percent_Identity=42.8115015974441, Blast_Score=266, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6322667, Length=207, Percent_Identity=38.6473429951691, Blast_Score=146, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6324511, Length=112, Percent_Identity=39.2857142857143, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI21355239, Length=315, Percent_Identity=45.0793650793651, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI45551540, Length=337, Percent_Identity=42.1364985163205, Blast_Score=279, Evalue=2e-75,
Organism=Drosophila melanogaster, GI24651458, Length=354, Percent_Identity=35.3107344632768, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI24651456, Length=354, Percent_Identity=35.3107344632768, Blast_Score=207, Evalue=9e-54,
Organism=Drosophila melanogaster, GI281362873, Length=354, Percent_Identity=35.3107344632768, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24651454, Length=354, Percent_Identity=35.3107344632768, Blast_Score=207, Evalue=1e-53,
Organism=Drosophila melanogaster, GI24651462, Length=373, Percent_Identity=33.7801608579088, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24651464, Length=373, Percent_Identity=33.7801608579088, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI45552010, Length=373, Percent_Identity=32.7077747989276, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34589; Mature: 34458

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYLAIFTGNAHPELAKTVADHLHIPLGKADITRFSDGEIAVEIKEHVRGKDVFIMQPTC
CCEEEEEECCCCHHHHHHHHHHHCCCCCCHHCCEECCCCEEEEEHHHCCCCEEEEECCCC
APTNDNLMEIMMMADALRRSSAGRITAVMPYFGYARQDRRPRSARVPISAKVVADMLNIV
CCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHCCHHHCCCCCCCCCCCHHHHHHHHHHHH
SIDRVMTVDLHSDQIQGFFDIPVDNIYGTPVLLNDLQKQDYDNIMVVSPDVGGVVRARAM
HHCCEEEEEECHHHCCEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
AKQLGDTDMAIIDKRRARANESQVMHIIGDVRDRDCVIVDDMVDTAGTLCKAAEALKANG
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCCC
ARRVVAYITHPVLSGNALKNISESELDEIVVTDTIPLSDAAKACSKIRQVSIAPMLAESL
CCEEEEEECCCCCCCHHHHCCCHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRINNEESISAMFDA
HHCCCCHHHHHCCCC
>Mature Secondary Structure 
PYLAIFTGNAHPELAKTVADHLHIPLGKADITRFSDGEIAVEIKEHVRGKDVFIMQPTC
CEEEEEECCCCHHHHHHHHHHHCCCCCCHHCCEECCCCEEEEEHHHCCCCEEEEECCCC
APTNDNLMEIMMMADALRRSSAGRITAVMPYFGYARQDRRPRSARVPISAKVVADMLNIV
CCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHCCHHHCCCCCCCCCCCHHHHHHHHHHHH
SIDRVMTVDLHSDQIQGFFDIPVDNIYGTPVLLNDLQKQDYDNIMVVSPDVGGVVRARAM
HHCCEEEEEECHHHCCEEEECCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
AKQLGDTDMAIIDKRRARANESQVMHIIGDVRDRDCVIVDDMVDTAGTLCKAAEALKANG
HHHHCCCCHHHHHHHHHCCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHCCC
ARRVVAYITHPVLSGNALKNISESELDEIVVTDTIPLSDAAKACSKIRQVSIAPMLAESL
CCEEEEEECCCCCCCHHHHCCCHHHHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
RRINNEESISAMFDA
HHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]