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The map label for this gene is tusA [H]

Identifier: 93004890

GI number: 93004890

Start: 68939

End: 69199

Strand: Reverse

Name: tusA [H]

Synonym: Pcryo_0059

Alternate gene names: 93004890

Gene position: 69199-68939 (Counterclockwise)

Preceding gene: 93004891

Following gene: 93004889

Centisome position: 2.26

GC content: 44.83

Gene sequence:

>261_bases
ATGACGACTTCAATATTAGACACGACTATTCACCATCATCTAGATACCCAAGGACTGATTTGTCCTGAGCCAGTCATGAT
GCTACATAAGACAATTCGTAAAGCGGCTGGCGGCGATGTGATTGAGATATTAGCCACTGATCCTGCCACTACGCGTGATA
TTCCTAATTTCTGCCGGCACTTGGGACATACCCTTGTCCATCAAGAAACGCTAAACGATGCGCCAACCTATCGTTATTTA
GTCAAGAAAAAGAGCGCTTAG

Upstream 100 bases:

>100_bases
AAAGATATTATCGCTATTGATGCAGAAAATAGGCCTTTATAAAATCGTTAGTAAACAAGTATTCAGCTACCAAACTTATC
TATTAGGTTTATAAAAAAAT

Downstream 100 bases:

>100_bases
CGCAATATATAATGCAGAAATATCTTTGCGTTATAAAAAATACATCATTCACATTTGAAAGGTAGGTCGCGTGTTAGCAA
CAGAAATACAGTACGTGCAA

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein A [H]

Number of amino acids: Translated: 86; Mature: 85

Protein sequence:

>86_residues
MTTSILDTTIHHHLDTQGLICPEPVMMLHKTIRKAAGGDVIEILATDPATTRDIPNFCRHLGHTLVHQETLNDAPTYRYL
VKKKSA

Sequences:

>Translated_86_residues
MTTSILDTTIHHHLDTQGLICPEPVMMLHKTIRKAAGGDVIEILATDPATTRDIPNFCRHLGHTLVHQETLNDAPTYRYL
VKKKSA
>Mature_85_residues
TTSILDTTIHHHLDTQGLICPEPVMMLHKTIRKAAGGDVIEILATDPATTRDIPNFCRHLGHTLVHQETLNDAPTYRYLV
KKKSA

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions. Interacts with iscS and stimulates its activity. Then, accepts a sulfur from iscS and transfers it in turn to tusD [H]

COG id: COG0425

COG function: function code O; Predicted redox protein, regulator of disulfide bond formation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0033 family. TusA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789881, Length=78, Percent_Identity=52.5641025641026, Blast_Score=83, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001455
- InterPro:   IPR022931 [H]

Pfam domain/function: PF01206 SirA [H]

EC number: NA

Molecular weight: Translated: 9614; Mature: 9483

Theoretical pI: Translated: 7.71; Mature: 7.71

Prosite motif: PS01148 UPF0033

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTSILDTTIHHHLDTQGLICPEPVMMLHKTIRKAAGGDVIEILATDPATTRDIPNFCRH
CCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
LGHTLVHQETLNDAPTYRYLVKKKSA
HHHHHHHHHHCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
TTSILDTTIHHHLDTQGLICPEPVMMLHKTIRKAAGGDVIEILATDPATTRDIPNFCRH
CCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHH
LGHTLVHQETLNDAPTYRYLVKKKSA
HHHHHHHHHHCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]