Click here to switch to the map view.

The map label for this gene is mpl [H]

Identifier: 93004871

GI number: 93004871

Start: 46979

End: 48496

Strand: Reverse

Name: mpl [H]

Synonym: Pcryo_0040

Alternate gene names: 93004871

Gene position: 48496-46979 (Counterclockwise)

Preceding gene: 93004872

Following gene: 93004870

Centisome position: 1.58

GC content: 45.32

Gene sequence:

>1518_bases
ATGCATATTCATATTCTTGGTATTTGTGGTACTTTTATGGGCTCACTGGCATTGCTAGCGCGCGAGCTTGGACATACGGT
CACGGGCTCAGATGCCAACGTTTATCCGCCGATGTCCACCCAACTGGAAAACGCGGGCGTGACCATTGAGCAAGGCTATC
TGGTAGAGCATTTGCAACCAGCACCAGATCTAGTCGTGGTTGGTAATGCCATGAAGCGCGGTATGGATGTCATCGAATAT
ATGCTGGACACAGGTCTACGCTATACCTCTGGACCCCAGTTTTTATCTGAGCAAGTATTGCAATCTCGCCATGTCATCGC
AGTGGCAGGTACTCATGGCAAGACCACGACGACGACCATGCTCGCGTGGATACTGCACTATGCCGGTATCGACACTGGGT
TTTTAATCGGTGGTGTGCCACTTGTTGATACTGCTGATGCACACTTGCAGCAAGTCTTTGCTCACAGCAGTTATTTGGGT
GCTGACAAAATTGATAATGACGATTCGGCAAATACTGGCTATTTCGTTATCGAAGCAGATGAATACGATTCTGCATTTTT
TGATAAGCGTTCAAAGTTCGTACATTATCGTCCGCGTACGGCTATTTTAAATAACCTTGAATTTGATCATGCGGATATTT
TTGCGGATCTTGATGCTATTCAGACCCAATTTCATCATATGGTGCGCATGATTCCAAGCACTGGCAAAATCATTATGCCC
ACAGCGACTGCCAGCTTAGAGGAAACGTTAGCGAAAGGTGTTTGGACACCCGTTTGGCGGACAGCAGTATTGGACTCAGC
GGCACATAATACTCATGCAACAGATAATACCTACGCAAAAGATAAGCATGTAAAAGATAGTAGCGATTGGCAAGCTGAAC
TTATCAGCGAAGATGGTGGTCAATTTGCTGTTAGTTTTGCGGCTGATATTGCTGATAAAGAAGCCACGGGAGTCGTCGAT
TGGTCGATGAGCGGCATTCACAATGTGAATAACGCCTTGGTTGCTGTCGCAGCGGCTTATAACATCGGTGTCAGTGTTAA
AACTGCCTGTGCGGCGTTATCAGCATTTGCCGGTATTAAACGTCGGATGGAGCTTATAGGTAATGTAAATGATATCTTGG
TCTTTGATGATTTCGCTCATCATCCAACGGCAATTACGACTACTTTGGATGGTGCTAAGAAAAAGCTAGCTGATAGACGA
CTGTGGGCAATTATTGAACCACGTAGTAATACCATGAAAATGGGCATTCATCAAGACAGCTTGGCTGAGTCTGCTGCTCT
CGCCGATCATACCTTATGGTATGAGCCGACAGGACTTGAGTGGGGTCTAAAAGAAGTCATTAATAATGCCACTATCGCAA
ATCCAGATATTGGCAGCCAACAGGTACTCTCTAGCGTAGAAGATATCATTACTCATATTTGCACATATGCCAAGGCTGGT
GATGCGATTGTGATTATGTCTAATGGCGGCTTTGAAGGTATTCATCAGCGTTTAGTAACCGCTTTAGCTAATAGTTAG

Upstream 100 bases:

>100_bases
TTTGCTAAGATAAATATTAGCTCATCCAAAATACAGCAATCTCTTTTATTGGCTTACGTTAGATTTTCATTCCTCTTATT
CAAGCTCGTACGGAACGTCT

Downstream 100 bases:

>100_bases
TCTGGCTCTATAACCCTGTAGAATAACAGGCGTAGCTCATTAATAATGAGCTACGCCTGTTTATTTCTTTTTATACCTAT
AAAAAAATATTTTTACTTTT

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MHIHILGICGTFMGSLALLARELGHTVTGSDANVYPPMSTQLENAGVTIEQGYLVEHLQPAPDLVVVGNAMKRGMDVIEY
MLDTGLRYTSGPQFLSEQVLQSRHVIAVAGTHGKTTTTTMLAWILHYAGIDTGFLIGGVPLVDTADAHLQQVFAHSSYLG
ADKIDNDDSANTGYFVIEADEYDSAFFDKRSKFVHYRPRTAILNNLEFDHADIFADLDAIQTQFHHMVRMIPSTGKIIMP
TATASLEETLAKGVWTPVWRTAVLDSAAHNTHATDNTYAKDKHVKDSSDWQAELISEDGGQFAVSFAADIADKEATGVVD
WSMSGIHNVNNALVAVAAAYNIGVSVKTACAALSAFAGIKRRMELIGNVNDILVFDDFAHHPTAITTTLDGAKKKLADRR
LWAIIEPRSNTMKMGIHQDSLAESAALADHTLWYEPTGLEWGLKEVINNATIANPDIGSQQVLSSVEDIITHICTYAKAG
DAIVIMSNGGFEGIHQRLVTALANS

Sequences:

>Translated_505_residues
MHIHILGICGTFMGSLALLARELGHTVTGSDANVYPPMSTQLENAGVTIEQGYLVEHLQPAPDLVVVGNAMKRGMDVIEY
MLDTGLRYTSGPQFLSEQVLQSRHVIAVAGTHGKTTTTTMLAWILHYAGIDTGFLIGGVPLVDTADAHLQQVFAHSSYLG
ADKIDNDDSANTGYFVIEADEYDSAFFDKRSKFVHYRPRTAILNNLEFDHADIFADLDAIQTQFHHMVRMIPSTGKIIMP
TATASLEETLAKGVWTPVWRTAVLDSAAHNTHATDNTYAKDKHVKDSSDWQAELISEDGGQFAVSFAADIADKEATGVVD
WSMSGIHNVNNALVAVAAAYNIGVSVKTACAALSAFAGIKRRMELIGNVNDILVFDDFAHHPTAITTTLDGAKKKLADRR
LWAIIEPRSNTMKMGIHQDSLAESAALADHTLWYEPTGLEWGLKEVINNATIANPDIGSQQVLSSVEDIITHICTYAKAG
DAIVIMSNGGFEGIHQRLVTALANS
>Mature_505_residues
MHIHILGICGTFMGSLALLARELGHTVTGSDANVYPPMSTQLENAGVTIEQGYLVEHLQPAPDLVVVGNAMKRGMDVIEY
MLDTGLRYTSGPQFLSEQVLQSRHVIAVAGTHGKTTTTTMLAWILHYAGIDTGFLIGGVPLVDTADAHLQQVFAHSSYLG
ADKIDNDDSANTGYFVIEADEYDSAFFDKRSKFVHYRPRTAILNNLEFDHADIFADLDAIQTQFHHMVRMIPSTGKIIMP
TATASLEETLAKGVWTPVWRTAVLDSAAHNTHATDNTYAKDKHVKDSSDWQAELISEDGGQFAVSFAADIADKEATGVVD
WSMSGIHNVNNALVAVAAAYNIGVSVKTACAALSAFAGIKRRMELIGNVNDILVFDDFAHHPTAITTTLDGAKKKLADRR
LWAIIEPRSNTMKMGIHQDSLAESAALADHTLWYEPTGLEWGLKEVINNATIANPDIGSQQVLSSVEDIITHICTYAKAG
DAIVIMSNGGFEGIHQRLVTALANS

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=506, Percent_Identity=47.0355731225296, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1786279, Length=415, Percent_Identity=28.9156626506024, Blast_Score=102, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 54744; Mature: 54744

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGSLALLARELGHTVTGSDANVYPPMSTQLENAGVTIEQGYLVEHLQP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCC
APDLVVVGNAMKRGMDVIEYMLDTGLRYTSGPQFLSEQVLQSRHVIAVAGTHGKTTTTTM
CCCEEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHH
LAWILHYAGIDTGFLIGGVPLVDTADAHLQQVFAHSSYLGADKIDNDDSANTGYFVIEAD
HHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECC
EYDSAFFDKRSKFVHYRPRTAILNNLEFDHADIFADLDAIQTQFHHMVRMIPSTGKIIMP
CCCHHHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TATASLEETLAKGVWTPVWRTAVLDSAAHNTHATDNTYAKDKHVKDSSDWQAELISEDGG
CCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
QFAVSFAADIADKEATGVVDWSMSGIHNVNNALVAVAAAYNIGVSVKTACAALSAFAGIK
EEEEEEHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RRMELIGNVNDILVFDDFAHHPTAITTTLDGAKKKLADRRLWAIIEPRSNTMKMGIHQDS
HHHHHHCCCCEEEEEECCCCCCCEEEEECCHHHHHHHCCEEEEEECCCCCCEEECCCHHH
LAESAALADHTLWYEPTGLEWGLKEVINNATIANPDIGSQQVLSSVEDIITHICTYAKAG
HHHHHHHHCCCEEECCCCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
DAIVIMSNGGFEGIHQRLVTALANS
CEEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHIHILGICGTFMGSLALLARELGHTVTGSDANVYPPMSTQLENAGVTIEQGYLVEHLQP
CEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEECCCHHHCCCC
APDLVVVGNAMKRGMDVIEYMLDTGLRYTSGPQFLSEQVLQSRHVIAVAGTHGKTTTTTM
CCCEEEECHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHH
LAWILHYAGIDTGFLIGGVPLVDTADAHLQQVFAHSSYLGADKIDNDDSANTGYFVIEAD
HHHHHHHHCCCCCCEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECC
EYDSAFFDKRSKFVHYRPRTAILNNLEFDHADIFADLDAIQTQFHHMVRMIPSTGKIIMP
CCCHHHHHCCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEE
TATASLEETLAKGVWTPVWRTAVLDSAAHNTHATDNTYAKDKHVKDSSDWQAELISEDGG
CCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCC
QFAVSFAADIADKEATGVVDWSMSGIHNVNNALVAVAAAYNIGVSVKTACAALSAFAGIK
EEEEEEHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
RRMELIGNVNDILVFDDFAHHPTAITTTLDGAKKKLADRRLWAIIEPRSNTMKMGIHQDS
HHHHHHCCCCEEEEEECCCCCCCEEEEECCHHHHHHHCCEEEEEECCCCCCEEECCCHHH
LAESAALADHTLWYEPTGLEWGLKEVINNATIANPDIGSQQVLSSVEDIITHICTYAKAG
HHHHHHHHCCCEEECCCCCCHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
DAIVIMSNGGFEGIHQRLVTALANS
CEEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]