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The map label for this gene is rlmF [H]

Identifier: 93004839

GI number: 93004839

Start: 9456

End: 10544

Strand: Reverse

Name: rlmF [H]

Synonym: Pcryo_0008

Alternate gene names: 93004839

Gene position: 10544-9456 (Counterclockwise)

Preceding gene: 93004840

Following gene: 93004833

Centisome position: 0.34

GC content: 44.9

Gene sequence:

>1089_bases
ATGAAATCTGCTAAAATGGTGCATTATATTCAAACATCAAAGCCCGATACTATGACCAGTAAGCCTATGAACCGTCGCCC
GACCGCCAATAATCACAGAAACAGATCGCCTGAAACTGCCAAAAAATTAGGACAGCTACATCCGCGCAATCCGCACCAAG
GTCGTTATGACTTTGAAGTATTGACTCGCGCTTTGCCTGAGCTTGCTAAGCACACCATTACCAATCCTAAAGGTGAGTCG
ACGATTAACTTCTCAGACAGTGCCGCCGTGCGCGTCTTGAATCAAGCGCTACTAGCAAATTATTATGGCGTGAAATTTTG
GGATATTCCTGAAGGTTATCTCTGCCCACCGATCCCTGGTCGTGCCGACTATATCCATTATATCGCTGACCTGCTCGCGC
AAACCACGCATGTGAATAAAGACAATACGCCACCAACTGGCAAAGAAATCCACGCCCTCGATATTGGCACGGGTGCTAGC
GCGATTTATCCGATTGTTGGTAGCCAAAGCTACGGTTGGCGATTTACCGCCTCAGACATTGATCCTATTTCGGTCAATAC
CGCCGCGCTGATTTGTGAAACCAATCCAAAGCTAAAAAGTGCTGTTAAAGTCAAACTACAGACCGAACCTAAATTTATTT
TTAAAAATATCATCGGTCGCCAAGATTACTTTGATGTGGTGGTCTGTAATCCGCCGTTTCATGCCTCATTAGAAGAAGCG
ATGGAAGCCAATAGTCGTAAGCAACATAACTTGCAACGACATCGCGGTAAGAATGAAAACGAACAAATTAGCCGCAGCTC
AACAAAGCCTGGCAATGCCGCGCAAAACCTAAACTTTGGTGGTCAGCATAAAGAGCTGTGGTCTGAGGGCGGTGAGATTG
CCTTTTTAACCAAAATGGCAAAAGAGAGTCAAGATTTCGCTGAGCATGTTGGCTGGTTTACGACACTGGTATCTAAGTCA
GAAAACGTAAAACCTCTGCAATTACTGTTGAAACAACTTGGTGTCGCCCAAATGCGTATCATCGAGATGAGTCAAGGTCA
AAAAAGTACTCGTATACTTGCATGGCGTTTTAATACTGATGAAGAGTAA

Upstream 100 bases:

>100_bases
TTCAGCGTTTTGAACATTGGATCGACCGCGTATTGGGTACAGCGATGATAATATTGGGATTAAATCTTATTTTACGTAGT
CGATAAGACGCTAGTGACTC

Downstream 100 bases:

>100_bases
ATAATAATTAAGATTATAAATAACTATAGTTTCACGTGAAACAAAAAAGCACCCCTTTAATGATCAAAGGGGTGCTTTTT
ATTGTAGAGAATTAATCCAA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKSAKMVHYIQTSKPDTMTSKPMNRRPTANNHRNRSPETAKKLGQLHPRNPHQGRYDFEVLTRALPELAKHTITNPKGES
TINFSDSAAVRVLNQALLANYYGVKFWDIPEGYLCPPIPGRADYIHYIADLLAQTTHVNKDNTPPTGKEIHALDIGTGAS
AIYPIVGSQSYGWRFTASDIDPISVNTAALICETNPKLKSAVKVKLQTEPKFIFKNIIGRQDYFDVVVCNPPFHASLEEA
MEANSRKQHNLQRHRGKNENEQISRSSTKPGNAAQNLNFGGQHKELWSEGGEIAFLTKMAKESQDFAEHVGWFTTLVSKS
ENVKPLQLLLKQLGVAQMRIIEMSQGQKSTRILAWRFNTDEE

Sequences:

>Translated_362_residues
MKSAKMVHYIQTSKPDTMTSKPMNRRPTANNHRNRSPETAKKLGQLHPRNPHQGRYDFEVLTRALPELAKHTITNPKGES
TINFSDSAAVRVLNQALLANYYGVKFWDIPEGYLCPPIPGRADYIHYIADLLAQTTHVNKDNTPPTGKEIHALDIGTGAS
AIYPIVGSQSYGWRFTASDIDPISVNTAALICETNPKLKSAVKVKLQTEPKFIFKNIIGRQDYFDVVVCNPPFHASLEEA
MEANSRKQHNLQRHRGKNENEQISRSSTKPGNAAQNLNFGGQHKELWSEGGEIAFLTKMAKESQDFAEHVGWFTTLVSKS
ENVKPLQLLLKQLGVAQMRIIEMSQGQKSTRILAWRFNTDEE
>Mature_362_residues
MKSAKMVHYIQTSKPDTMTSKPMNRRPTANNHRNRSPETAKKLGQLHPRNPHQGRYDFEVLTRALPELAKHTITNPKGES
TINFSDSAAVRVLNQALLANYYGVKFWDIPEGYLCPPIPGRADYIHYIADLLAQTTHVNKDNTPPTGKEIHALDIGTGAS
AIYPIVGSQSYGWRFTASDIDPISVNTAALICETNPKLKSAVKVKLQTEPKFIFKNIIGRQDYFDVVVCNPPFHASLEEA
MEANSRKQHNLQRHRGKNENEQISRSSTKPGNAAQNLNFGGQHKELWSEGGEIAFLTKMAKESQDFAEHVGWFTTLVSKS
ENVKPLQLLLKQLGVAQMRIIEMSQGQKSTRILAWRFNTDEE

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA [H]

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family [H]

Homologues:

Organism=Homo sapiens, GI122114654, Length=318, Percent_Identity=30.188679245283, Blast_Score=145, Evalue=8e-35,
Organism=Escherichia coli, GI87081786, Length=324, Percent_Identity=44.4444444444444, Blast_Score=293, Evalue=1e-80,
Organism=Caenorhabditis elegans, GI32564931, Length=319, Percent_Identity=28.8401253918495, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI32564933, Length=319, Percent_Identity=26.0188087774295, Blast_Score=111, Evalue=6e-25,
Organism=Drosophila melanogaster, GI281363415, Length=329, Percent_Identity=27.0516717325228, Blast_Score=114, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19922302, Length=329, Percent_Identity=27.0516717325228, Blast_Score=114, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR016909
- InterPro:   IPR010286 [H]

Pfam domain/function: PF05971 Methyltransf_10 [H]

EC number: =2.1.1.181 [H]

Molecular weight: Translated: 40676; Mature: 40676

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSAKMVHYIQTSKPDTMTSKPMNRRPTANNHRNRSPETAKKLGQLHPRNPHQGRYDFEV
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
LTRALPELAKHTITNPKGESTINFSDSAAVRVLNQALLANYYGVKFWDIPEGYLCPPIPG
HHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
RADYIHYIADLLAQTTHVNKDNTPPTGKEIHALDIGTGASAIYPIVGSQSYGWRFTASDI
CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEECCCC
DPISVNTAALICETNPKLKSAVKVKLQTEPKFIFKNIIGRQDYFDVVVCNPPFHASLEEA
CCCCCCCEEEEECCCCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHH
MEANSRKQHNLQRHRGKNENEQISRSSTKPGNAAQNLNFGGQHKELWSEGGEIAFLTKMA
HHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEHHHHH
KESQDFAEHVGWFTTLVSKSENVKPLQLLLKQLGVAQMRIIEMSQGQKSTRILAWRFNTD
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHEEEEECCCCCCCCEEEEEECCCC
EE
CC
>Mature Secondary Structure
MKSAKMVHYIQTSKPDTMTSKPMNRRPTANNHRNRSPETAKKLGQLHPRNPHQGRYDFEV
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHH
LTRALPELAKHTITNPKGESTINFSDSAAVRVLNQALLANYYGVKFWDIPEGYLCPPIPG
HHHHHHHHHHHHCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCC
RADYIHYIADLLAQTTHVNKDNTPPTGKEIHALDIGTGASAIYPIVGSQSYGWRFTASDI
CHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEEEECCCCCCCEEECCCC
DPISVNTAALICETNPKLKSAVKVKLQTEPKFIFKNIIGRQDYFDVVVCNPPFHASLEEA
CCCCCCCEEEEECCCCCCCCEEEEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCCHHHH
MEANSRKQHNLQRHRGKNENEQISRSSTKPGNAAQNLNFGGQHKELWSEGGEIAFLTKMA
HHHCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEHHHHH
KESQDFAEHVGWFTTLVSKSENVKPLQLLLKQLGVAQMRIIEMSQGQKSTRILAWRFNTD
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCHHHEEEEECCCCCCCCEEEEEECCCC
EE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA