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The map label for this gene is amtB [C]

Identifier: 92115277

GI number: 92115277

Start: 3526970

End: 3528220

Strand: Direct

Name: amtB [C]

Synonym: Csal_3162

Alternate gene names: 92115277

Gene position: 3526970-3528220 (Clockwise)

Preceding gene: 92115276

Following gene: 92115278

Centisome position: 95.41

GC content: 64.11

Gene sequence:

>1251_bases
ATGACTGAAGATATTACACAGCTATCGTACGCGCTCGACACCTTCTACTTCCTGGTCTGCGCCGTACTGGTCATGTGGAT
GGCCGCCGGTTTCGCCATGCTCGAGGCCGGTCTCGTCCGCGCCAAGAACACGGCGGAGATCCTGACCAAGAACGTGGTGT
TGTTCGCCATCTCTTGCACGATGTATCTGCTGTTCAGCTACCACTTGATGTATTCCAGTGGCGCTGGCGGCTTCATGCCG
AGCCTGGGCTTCCTGCTGGGCGCCGAGAACACGGCCGACCAGGTCCTGGGCAGCAACGGCGACCTCTACTATTCCAACCG
GGCCGACTTCTTCTTCCAGGTGGTGTTCGTGGCGACCGCCATGTCGATCGTCTCCGGGGCCGTGGCGGAACGCATGAAAC
TCTGGGCGTTTCTGGCCTTCGCCGTGGTGATGACCGCGATCATCTATCCGACGTCCGGCTACTGGACCTGGGGCGGCGGC
TGGCTCGCCGAGCTGGGGTATTCCGACTATGCCGGCTCGGGCATCGTCCACATGGCCGGGGCGGCCGCGGCTCTGGCCGG
GGTGCTGGTACTCGGGCCGCGCAAGGGCAAGTACGGCAAGAGCGGCGCGGTCTACGCCATTCCCGGTGCCAACCTGCCGC
TGGCGACGCTGGGGACCTTCATCCTGTGGATGGGCTGGTTCGGCTTCAACGGCGGCAGCGAGCTGATGGTCTCCAACATC
GATTCCGCCAACAACATGGCGCAGGTACTGGTCAACACCAATGCCGCCGCCGCCGGGGGCGTGATCGCCGCGCTGATCCT
CGCCAAGATCTGGTTCCGCAAGGCGGACCTGACCATGGCCCTCAACGGTGCCATCGCCGGTCTGGTCGCGATCACCGCCG
ATCCGCTGTCACCCACCGGCCTGGGCGCCACGCTCATCGGTGCCGTCGGCGGGTTGCTGGTGGTCATCTCCATCGTGGCG
CTGGACAAGCTGAAGATCGACGATCCGGTCGGTGCCATCTCCGCACACGGCGTGGCCGGCCTGTGGGGCGTTCTGGTGGT
GCCTGTCACCAACGCCAGCGCCTCCTTCGGCTCACAGATCATCGGTGCCGTCGCCATCTTCCTGTGGTGCTTCATCGCGA
GCCTGATCGTGTGGCTGATCCTCAAGGCCGTGATGGGCGTCCGGGTCAGCGAGGAAGAAGAATACGAAGGGGTGGACCTG
GCGGAATGCGGCCTGGAAGCCTACCCCGAATTTTCCGTCGGCAAGAAGTAA

Upstream 100 bases:

>100_bases
CAAGCTCTTCGTCACGCCGCTCGAAGACGTCATTCGAATCCGTACCGGTGAACGCGGGGCCGACGCCGTATAACGGCGCG
GGCTGAAGGAGAGATAATAA

Downstream 100 bases:

>100_bases
GAGAGCCGACATACCGAGTGAGCTGGCGTGCTCCCCTGGCCCCCTTCGGGGGGCCTTTTTTTAATCCCGCGACCAGCGGT
GTATGCTTGAAAGCATGATC

Product: Rh-like protein/ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTEDITQLSYALDTFYFLVCAVLVMWMAAGFAMLEAGLVRAKNTAEILTKNVVLFAISCTMYLLFSYHLMYSSGAGGFMP
SLGFLLGAENTADQVLGSNGDLYYSNRADFFFQVVFVATAMSIVSGAVAERMKLWAFLAFAVVMTAIIYPTSGYWTWGGG
WLAELGYSDYAGSGIVHMAGAAAALAGVLVLGPRKGKYGKSGAVYAIPGANLPLATLGTFILWMGWFGFNGGSELMVSNI
DSANNMAQVLVNTNAAAAGGVIAALILAKIWFRKADLTMALNGAIAGLVAITADPLSPTGLGATLIGAVGGLLVVISIVA
LDKLKIDDPVGAISAHGVAGLWGVLVVPVTNASASFGSQIIGAVAIFLWCFIASLIVWLILKAVMGVRVSEEEEYEGVDL
AECGLEAYPEFSVGKK

Sequences:

>Translated_416_residues
MTEDITQLSYALDTFYFLVCAVLVMWMAAGFAMLEAGLVRAKNTAEILTKNVVLFAISCTMYLLFSYHLMYSSGAGGFMP
SLGFLLGAENTADQVLGSNGDLYYSNRADFFFQVVFVATAMSIVSGAVAERMKLWAFLAFAVVMTAIIYPTSGYWTWGGG
WLAELGYSDYAGSGIVHMAGAAAALAGVLVLGPRKGKYGKSGAVYAIPGANLPLATLGTFILWMGWFGFNGGSELMVSNI
DSANNMAQVLVNTNAAAAGGVIAALILAKIWFRKADLTMALNGAIAGLVAITADPLSPTGLGATLIGAVGGLLVVISIVA
LDKLKIDDPVGAISAHGVAGLWGVLVVPVTNASASFGSQIIGAVAIFLWCFIASLIVWLILKAVMGVRVSEEEEYEGVDL
AECGLEAYPEFSVGKK
>Mature_415_residues
TEDITQLSYALDTFYFLVCAVLVMWMAAGFAMLEAGLVRAKNTAEILTKNVVLFAISCTMYLLFSYHLMYSSGAGGFMPS
LGFLLGAENTADQVLGSNGDLYYSNRADFFFQVVFVATAMSIVSGAVAERMKLWAFLAFAVVMTAIIYPTSGYWTWGGGW
LAELGYSDYAGSGIVHMAGAAAALAGVLVLGPRKGKYGKSGAVYAIPGANLPLATLGTFILWMGWFGFNGGSELMVSNID
SANNMAQVLVNTNAAAAGGVIAALILAKIWFRKADLTMALNGAIAGLVAITADPLSPTGLGATLIGAVGGLLVVISIVAL
DKLKIDDPVGAISAHGVAGLWGVLVVPVTNASASFGSQIIGAVAIFLWCFIASLIVWLILKAVMGVRVSEEEEYEGVDLA
ECGLEAYPEFSVGKK

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=414, Percent_Identity=32.8502415458937, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17550180, Length=458, Percent_Identity=32.0960698689956, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17550182, Length=450, Percent_Identity=29.7777777777778, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI133931100, Length=441, Percent_Identity=28.7981859410431, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI32564042, Length=432, Percent_Identity=28.9351851851852, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6325396, Length=441, Percent_Identity=28.1179138321995, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324187, Length=415, Percent_Identity=28.6746987951807, Blast_Score=115, Evalue=9e-27,
Organism=Saccharomyces cerevisiae, GI6321559, Length=446, Percent_Identity=26.6816143497758, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI161078314, Length=414, Percent_Identity=33.5748792270531, Blast_Score=175, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 43556; Mature: 43425

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEDITQLSYALDTFYFLVCAVLVMWMAAGFAMLEAGLVRAKNTAEILTKNVVLFAISCT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MYLLFSYHLMYSSGAGGFMPSLGFLLGAENTADQVLGSNGDLYYSNRADFFFQVVFVATA
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCHHHHHHHHHHHHH
MSIVSGAVAERMKLWAFLAFAVVMTAIIYPTSGYWTWGGGWLAELGYSDYAGSGIVHMAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCHHHHHH
AAAALAGVLVLGPRKGKYGKSGAVYAIPGANLPLATLGTFILWMGWFGFNGGSELMVSNI
HHHHHHHHHEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
DSANNMAQVLVNTNAAAAGGVIAALILAKIWFRKADLTMALNGAIAGLVAITADPLSPTG
CCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHEEECCCCCCCC
LGATLIGAVGGLLVVISIVALDKLKIDDPVGAISAHGVAGLWGVLVVPVTNASASFGSQI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHEEEECCCCHHHHHHH
IGAVAIFLWCFIASLIVWLILKAVMGVRVSEEEEYEGVDLAECGLEAYPEFSVGKK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHCCCCCCCCCC
>Mature Secondary Structure 
TEDITQLSYALDTFYFLVCAVLVMWMAAGFAMLEAGLVRAKNTAEILTKNVVLFAISCT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MYLLFSYHLMYSSGAGGFMPSLGFLLGAENTADQVLGSNGDLYYSNRADFFFQVVFVATA
HHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCHHHHHHHHHHHHH
MSIVSGAVAERMKLWAFLAFAVVMTAIIYPTSGYWTWGGGWLAELGYSDYAGSGIVHMAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCHHHHHH
AAAALAGVLVLGPRKGKYGKSGAVYAIPGANLPLATLGTFILWMGWFGFNGGSELMVSNI
HHHHHHHHHEECCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEEECC
DSANNMAQVLVNTNAAAAGGVIAALILAKIWFRKADLTMALNGAIAGLVAITADPLSPTG
CCCCCHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHHHHEEECCCCCCCC
LGATLIGAVGGLLVVISIVALDKLKIDDPVGAISAHGVAGLWGVLVVPVTNASASFGSQI
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCHHHHHHHHEEEECCCCHHHHHHH
IGAVAIFLWCFIASLIVWLILKAVMGVRVSEEEEYEGVDLAECGLEAYPEFSVGKK
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]