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The map label for this gene is thiD [H]

Identifier: 92115248

GI number: 92115248

Start: 3489461

End: 3490294

Strand: Direct

Name: thiD [H]

Synonym: Csal_3133

Alternate gene names: 92115248

Gene position: 3489461-3490294 (Clockwise)

Preceding gene: 92115234

Following gene: 92115249

Centisome position: 94.4

GC content: 69.3

Gene sequence:

>834_bases
ATGACCTCAGCATCCGCGCCCCCCAATGTCCTCACCATCGCCGGCTCCGACCCTTCCGGCGGCGCCGGCCTGCAGGGCGA
CATCAAGACCTTCTCGGCGTTGGGCACTTATGCCACCAATGTCGTCACGGCGGTGATCGCCCAGAACACCCAGGGCGTGC
AGGCCGTGCATCCGGTACCCGCGGATGTCATCGGCGAACAGCTCGACAACCTGCTGGGTGACGTACGCATCGAGGCGGTC
AAGATCGGCATGGTGGCCAGCGAGACGGTCGCCGCGACGATTCGCGATGCCCTGCACCGGCATCGTCCCCGCTGGATCGT
GCTCGACCCGGTCATGGTCGCCAAGAGCGGCGACATCCTGGTCGATCGCGCCGGCATCCGCGCGGTACGCGATATCCTGG
TTCCCCTGGCCGATGTCATCACCCCCAACCTGCCCGAGGCCGCCGTGCTGCTGGACGCCGAAACGCCCCGCACCACCGAG
GCGATGGCCACCCTGCTGCCCGAGCTGACACGCCTGGGCGCCCCGTACGTGGTACTCAAGGGCGGCCACCTCAGTGGCGC
GAACTGCCCGGACCTGCTCGCCACGCCGCAAGGCGTGGAATGGCTGGAAGCGCCGCGCATCGCCACCGACAACCTGCATG
GCACCGGCTGCGCGCTCTCCTCGGCGGTCGCCGCGCACCTGGCGCACTTGCCGCACACGGTCAGCGACCACGACGCCATT
CACGCGGCGATCAGCGATGCCAAGCGCTGGCTGCACACCGCTCTGGACGCCAGCACGCGACTGGCGGTGGGCCAGGGGCG
CGGCCCGGTGCATCATTTCCACGCCTGGTGGTGA

Upstream 100 bases:

>100_bases
CTGCTCACCCGTCGTTTCCACGCTATCCTGTGCGGATGCCAACTGCCGAGCACGGCCACACAGCGACGCACATATTCCGC
GAGACACAGGAACCCAGCGC

Downstream 100 bases:

>100_bases
AAACCGCGGCCGCACGGCGCTCGTCGGCAGTATCACGACCTCGTGAATCGATGATGGCCACGGACTTGCAGAGCCCGCCG
GGGGCAGCCATGCTTTGCTG

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTSASAPPNVLTIAGSDPSGGAGLQGDIKTFSALGTYATNVVTAVIAQNTQGVQAVHPVPADVIGEQLDNLLGDVRIEAV
KIGMVASETVAATIRDALHRHRPRWIVLDPVMVAKSGDILVDRAGIRAVRDILVPLADVITPNLPEAAVLLDAETPRTTE
AMATLLPELTRLGAPYVVLKGGHLSGANCPDLLATPQGVEWLEAPRIATDNLHGTGCALSSAVAAHLAHLPHTVSDHDAI
HAAISDAKRWLHTALDASTRLAVGQGRGPVHHFHAWW

Sequences:

>Translated_277_residues
MTSASAPPNVLTIAGSDPSGGAGLQGDIKTFSALGTYATNVVTAVIAQNTQGVQAVHPVPADVIGEQLDNLLGDVRIEAV
KIGMVASETVAATIRDALHRHRPRWIVLDPVMVAKSGDILVDRAGIRAVRDILVPLADVITPNLPEAAVLLDAETPRTTE
AMATLLPELTRLGAPYVVLKGGHLSGANCPDLLATPQGVEWLEAPRIATDNLHGTGCALSSAVAAHLAHLPHTVSDHDAI
HAAISDAKRWLHTALDASTRLAVGQGRGPVHHFHAWW
>Mature_276_residues
TSASAPPNVLTIAGSDPSGGAGLQGDIKTFSALGTYATNVVTAVIAQNTQGVQAVHPVPADVIGEQLDNLLGDVRIEAVK
IGMVASETVAATIRDALHRHRPRWIVLDPVMVAKSGDILVDRAGIRAVRDILVPLADVITPNLPEAAVLLDAETPRTTEA
MATLLPELTRLGAPYVVLKGGHLSGANCPDLLATPQGVEWLEAPRIATDNLHGTGCALSSAVAAHLAHLPHTVSDHDAIH
AAISDAKRWLHTALDASTRLAVGQGRGPVHHFHAWW

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P. Shows no activity with pyridoxal, pyridoxamine or pyridoxine [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=269, Percent_Identity=47.9553903345725, Blast_Score=248, Evalue=4e-67,
Organism=Saccharomyces cerevisiae, GI6325378, Length=283, Percent_Identity=28.9752650176678, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324517, Length=284, Percent_Identity=29.9295774647887, Blast_Score=112, Evalue=8e-26,
Organism=Saccharomyces cerevisiae, GI6324997, Length=291, Percent_Identity=30.9278350515464, Blast_Score=112, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013749
- InterPro:   IPR004399 [H]

Pfam domain/function: PF08543 Phos_pyr_kin [H]

EC number: =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 28990; Mature: 28859

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSASAPPNVLTIAGSDPSGGAGLQGDIKTFSALGTYATNVVTAVIAQNTQGVQAVHPVP
CCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
ADVIGEQLDNLLGDVRIEAVKIGMVASETVAATIRDALHRHRPRWIVLDPVMVAKSGDIL
HHHHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCEE
VDRAGIRAVRDILVPLADVITPNLPEAAVLLDAETPRTTEAMATLLPELTRLGAPYVVLK
EECHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEE
GGHLSGANCPDLLATPQGVEWLEAPRIATDNLHGTGCALSSAVAAHLAHLPHTVSDHDAI
CCCCCCCCCCHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
HAAISDAKRWLHTALDASTRLAVGQGRGPVHHFHAWW
HHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TSASAPPNVLTIAGSDPSGGAGLQGDIKTFSALGTYATNVVTAVIAQNTQGVQAVHPVP
CCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCC
ADVIGEQLDNLLGDVRIEAVKIGMVASETVAATIRDALHRHRPRWIVLDPVMVAKSGDIL
HHHHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHCCCCEEEECCEEEECCCCEE
VDRAGIRAVRDILVPLADVITPNLPEAAVLLDAETPRTTEAMATLLPELTRLGAPYVVLK
EECHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEE
GGHLSGANCPDLLATPQGVEWLEAPRIATDNLHGTGCALSSAVAAHLAHLPHTVSDHDAI
CCCCCCCCCCHHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHH
HAAISDAKRWLHTALDASTRLAVGQGRGPVHHFHAWW
HHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10075431; 9097040; 9278503 [H]