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The map label for this gene is panB

Identifier: 92115180

GI number: 92115180

Start: 3414437

End: 3415228

Strand: Direct

Name: panB

Synonym: Csal_3065

Alternate gene names: 92115180

Gene position: 3414437-3415228 (Clockwise)

Preceding gene: 92115179

Following gene: 92115181

Centisome position: 92.37

GC content: 65.4

Gene sequence:

>792_bases
ATGAAAACCGTCACCTTGAGCCGTCTCAATGCCTTCAAGCATGCCCAAGAGCCGTTCAGCTGCCTGACGGCCTACGATGC
CTCGTTCGCCCAGCATGCCGATGCAGCCGGCATCGATGTCCTGCTGGTCGGCGACTCGTTGGGCATGGTGCTTCAGGGCC
ATGCCAGCACGCTTCCCGTGACGCTCGACGACATCCTCTATCACACGCGCTGCGTGGCGCGCGGCAAGCAACGCAGCCTG
TTGATGGTCGACTTGCCCTTCATGAGCAACGCCGACACCGGCCAATTGCTGCGTGACGCCGGTGCCTTGATGCGGGCCGG
CGCCGAGCTGGTCAAGATCGAAGGCGCGGGGTGGATGCACGAGGGAGTCCGCGAGCTCACGCGGCGCGGCGTGCCGGTGT
GCGCGCATCTCGGCCTGACGCCACAGAGCGTGCATCAGTTCGGCGGCTACAAGGTACAAGGCCGCGAGCAGGACGATGCC
CAGCGCATCATCGACGATGCCCGCCTGCTGGTCGAGGCCGGTGCCTCGGTGATCCTGCTGGAATGCGTGCCCGCCGCACT
CGGCCGCGCCGTGCGCGACGCCGTGGACGTTCCCGTGATCGGTATCGGCGCCGGCCCCGAGGTGGATGGGCAGATCCTGG
TCATGCACGACGTGATCGGCGTGACGCATGGACGTCCTCCACGCTTCGCCAAGAATTTTCTGGCGGGTCGTGACAGCATC
CAGGCGGCGTTCGAAGCCTACCGCGATGCCGTCAAGACGCGTCAATTTCCCGCCGAGGAGCATTGTTTCTGA

Upstream 100 bases:

>100_bases
CCGATTTGTTACCGATCCACACTCCCACCACCCTCGGGTAACGCCTGCGGTAACACTTGCCGTCCGGGTCCGCCAGGACC
GACAAACCACGAGACTTTCC

Downstream 100 bases:

>100_bases
TGTTGACGCTGTCCGACATCGGCGAGCTGCGCAATCATCTGCATGCGGCGCGGCGTGATGCCAAGCGTATTGCGCTCGTT
CCCACCATGGGCAACCTGCA

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 263; Mature: 263

Protein sequence:

>263_residues
MKTVTLSRLNAFKHAQEPFSCLTAYDASFAQHADAAGIDVLLVGDSLGMVLQGHASTLPVTLDDILYHTRCVARGKQRSL
LMVDLPFMSNADTGQLLRDAGALMRAGAELVKIEGAGWMHEGVRELTRRGVPVCAHLGLTPQSVHQFGGYKVQGREQDDA
QRIIDDARLLVEAGASVILLECVPAALGRAVRDAVDVPVIGIGAGPEVDGQILVMHDVIGVTHGRPPRFAKNFLAGRDSI
QAAFEAYRDAVKTRQFPAEEHCF

Sequences:

>Translated_263_residues
MKTVTLSRLNAFKHAQEPFSCLTAYDASFAQHADAAGIDVLLVGDSLGMVLQGHASTLPVTLDDILYHTRCVARGKQRSL
LMVDLPFMSNADTGQLLRDAGALMRAGAELVKIEGAGWMHEGVRELTRRGVPVCAHLGLTPQSVHQFGGYKVQGREQDDA
QRIIDDARLLVEAGASVILLECVPAALGRAVRDAVDVPVIGIGAGPEVDGQILVMHDVIGVTHGRPPRFAKNFLAGRDSI
QAAFEAYRDAVKTRQFPAEEHCF
>Mature_263_residues
MKTVTLSRLNAFKHAQEPFSCLTAYDASFAQHADAAGIDVLLVGDSLGMVLQGHASTLPVTLDDILYHTRCVARGKQRSL
LMVDLPFMSNADTGQLLRDAGALMRAGAELVKIEGAGWMHEGVRELTRRGVPVCAHLGLTPQSVHQFGGYKVQGREQDDA
QRIIDDARLLVEAGASVILLECVPAALGRAVRDAVDVPVIGIGAGPEVDGQILVMHDVIGVTHGRPPRFAKNFLAGRDSI
QAAFEAYRDAVKTRQFPAEEHCF

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=263, Percent_Identity=53.2319391634981, Blast_Score=281, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6319653, Length=256, Percent_Identity=36.328125, Blast_Score=163, Evalue=3e-41,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_CHRSD (Q1QSZ9)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_575108.1
- ProteinModelPortal:   Q1QSZ9
- SMR:   Q1QSZ9
- STRING:   Q1QSZ9
- GeneID:   4028869
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_3065
- NMPDR:   fig|290398.4.peg.2563
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- BioCyc:   CSAL290398:CSAL_3065-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28345; Mature: 28345

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 181-181 BINDING 84-84 BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVTLSRLNAFKHAQEPFSCLTAYDASFAQHADAAGIDVLLVGDSLGMVLQGHASTLPV
CCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCCE
TLDDILYHTRCVARGKQRSLLMVDLPFMSNADTGQLLRDAGALMRAGAELVKIEGAGWMH
EHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHH
EGVRELTRRGVPVCAHLGLTPQSVHQFGGYKVQGREQDDAQRIIDDARLLVEAGASVILL
HHHHHHHHCCCCEEEECCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEH
ECVPAALGRAVRDAVDVPVIGIGAGPEVDGQILVMHDVIGVTHGRPPRFAKNFLAGRDSI
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEHHHCCCCCCHHHHHHHHCCCHHH
QAAFEAYRDAVKTRQFPAEEHCF
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKTVTLSRLNAFKHAQEPFSCLTAYDASFAQHADAAGIDVLLVGDSLGMVLQGHASTLPV
CCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECCCCCCCE
TLDDILYHTRCVARGKQRSLLMVDLPFMSNADTGQLLRDAGALMRAGAELVKIEGAGWMH
EHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHH
EGVRELTRRGVPVCAHLGLTPQSVHQFGGYKVQGREQDDAQRIIDDARLLVEAGASVILL
HHHHHHHHCCCCEEEECCCCHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCEEEH
ECVPAALGRAVRDAVDVPVIGIGAGPEVDGQILVMHDVIGVTHGRPPRFAKNFLAGRDSI
HHHHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEEEHHHCCCCCCHHHHHHHHCCCHHH
QAAFEAYRDAVKTRQFPAEEHCF
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA