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The map label for this gene is yogA [H]

Identifier: 92115157

GI number: 92115157

Start: 3386891

End: 3387862

Strand: Direct

Name: yogA [H]

Synonym: Csal_3042

Alternate gene names: 92115157

Gene position: 3386891-3387862 (Clockwise)

Preceding gene: 92115156

Following gene: 92115160

Centisome position: 91.62

GC content: 67.49

Gene sequence:

>972_bases
ATGTACGCCATTGCGATCGAAGACCAGAGACTCGTCTGGCGGGAGCAAGAAGCGCCATCGAGCATTGCCGCCGACGCCGT
GCGTATCGACGTCGCCTGGGCCGGCATGAACCGCGCGGATGCCATGCAGCGTGCGGGGCACTACCCGCCTCCCGAGGGTG
AAAGCGAGATCCCCGGCCTGGAGGTCAGCGGCACGATCGCCGAAGTGGGTCGTGGCGTGGAACGCTTCAAGCCCGGCGAC
AAGGTCTGCGCCTTGCTCGCCGGGGGCGGCTATGCCGAGCAGGTCGTGGTCGCCGAGGCACAGGTGTTGTCCATTCCGAC
GGGGCTGGGCCTGCGTGAAGCCGCCGCCCTGCCGGAAGTCTTCGCCACGGCATGGCTCAACCTGTACATGGAAGGCGCCG
TGCAACGCGGTGAGCGGGTGGTGCTGCACGCGGGAGCCAGCGGCGTGGGCACGGCGGCCATTCAACTTTGCCGAGCCTTC
GGCAATCCCTGCTTCGTGACCGTCGGCAGCCAGGCCAAGCTGGAGGCCTGTCAGCGCCTGGGCGCCGACGCCGGCTGGAA
CCGACACGACGGCAGCTTCGTCGAGCCCATCAATGCCTGGGGCGGAGCCGACATGATTCTCGATCCGGTGGGCGGCGAAT
ACATCGCGCAGGACCTGCAGATTCTCAACGCCGAGGGACGCCTGGTGGTGATCGGCCCGATGGCAGGCCGCGACGCGCAG
GTCGACCTCGCCCGGCTGCTCATGAAGCGTCAGCGCATCATCGGCTCGACGCTCCGCTCGCGTTCGCCCCGTGCCAAGGG
CGCCATTCTCGATGCGCTTCACGCCCACGTCTGGCCCAAAGTCACCAGCGGCGAGATCACCCCGTTGATCGACCGTGTCT
GGCCCATTCAGGAAGCCGACACGGCGATGCGCCACCTGACCGACAACGCCAACACGGGCAAGATACTGCTCAGCGTGAGC
GGGGAAGGCTAG

Upstream 100 bases:

>100_bases
CGCGCATCACGCACAACCCGCGACCTCGTGAGAGGAGCGTTCGACACTGTCGGGAGCATGCCGCTCAACCGTGGATCAAC
CGTTCAGGAGGAGACGCTCG

Downstream 100 bases:

>100_bases
CCGACATAGCGGCCGATCGGCGGCAGAGCGTATGGGCCAGCGTCAGGCGAGCCCCGCGGCGTCGCGGGTACGCTGGTAGG
TCAGTTGCAGCAGCCGCGCA

Product: zinc-binding alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MYAIAIEDQRLVWREQEAPSSIAADAVRIDVAWAGMNRADAMQRAGHYPPPEGESEIPGLEVSGTIAEVGRGVERFKPGD
KVCALLAGGGYAEQVVVAEAQVLSIPTGLGLREAAALPEVFATAWLNLYMEGAVQRGERVVLHAGASGVGTAAIQLCRAF
GNPCFVTVGSQAKLEACQRLGADAGWNRHDGSFVEPINAWGGADMILDPVGGEYIAQDLQILNAEGRLVVIGPMAGRDAQ
VDLARLLMKRQRIIGSTLRSRSPRAKGAILDALHAHVWPKVTSGEITPLIDRVWPIQEADTAMRHLTDNANTGKILLSVS
GEG

Sequences:

>Translated_323_residues
MYAIAIEDQRLVWREQEAPSSIAADAVRIDVAWAGMNRADAMQRAGHYPPPEGESEIPGLEVSGTIAEVGRGVERFKPGD
KVCALLAGGGYAEQVVVAEAQVLSIPTGLGLREAAALPEVFATAWLNLYMEGAVQRGERVVLHAGASGVGTAAIQLCRAF
GNPCFVTVGSQAKLEACQRLGADAGWNRHDGSFVEPINAWGGADMILDPVGGEYIAQDLQILNAEGRLVVIGPMAGRDAQ
VDLARLLMKRQRIIGSTLRSRSPRAKGAILDALHAHVWPKVTSGEITPLIDRVWPIQEADTAMRHLTDNANTGKILLSVS
GEG
>Mature_323_residues
MYAIAIEDQRLVWREQEAPSSIAADAVRIDVAWAGMNRADAMQRAGHYPPPEGESEIPGLEVSGTIAEVGRGVERFKPGD
KVCALLAGGGYAEQVVVAEAQVLSIPTGLGLREAAALPEVFATAWLNLYMEGAVQRGERVVLHAGASGVGTAAIQLCRAF
GNPCFVTVGSQAKLEACQRLGADAGWNRHDGSFVEPINAWGGADMILDPVGGEYIAQDLQILNAEGRLVVIGPMAGRDAQ
VDLARLLMKRQRIIGSTLRSRSPRAKGAILDALHAHVWPKVTSGEITPLIDRVWPIQEADTAMRHLTDNANTGKILLSVS
GEG

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI22538446, Length=316, Percent_Identity=41.1392405063291, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI22538444, Length=316, Percent_Identity=41.1392405063291, Blast_Score=220, Evalue=1e-57,
Organism=Homo sapiens, GI18379349, Length=303, Percent_Identity=31.023102310231, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI194239674, Length=296, Percent_Identity=28.0405405405405, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI13236495, Length=296, Percent_Identity=28.0405405405405, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI24308257, Length=314, Percent_Identity=26.1146496815287, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI41872631, Length=316, Percent_Identity=25.9493670886076, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI194239676, Length=294, Percent_Identity=26.530612244898, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI47519420, Length=301, Percent_Identity=29.5681063122924, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI28557745, Length=311, Percent_Identity=25.08038585209, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI197927207, Length=196, Percent_Identity=25.5102040816327, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1790485, Length=312, Percent_Identity=27.8846153846154, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789651, Length=244, Percent_Identity=27.4590163934426, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=278, Percent_Identity=25.8992805755396, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI212642053, Length=339, Percent_Identity=25.3687315634218, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI71987554, Length=150, Percent_Identity=32.6666666666667, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6319520, Length=323, Percent_Identity=24.1486068111455, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24581345, Length=333, Percent_Identity=25.5255255255255, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19920632, Length=316, Percent_Identity=25, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI221330659, Length=316, Percent_Identity=25, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 34354; Mature: 34354

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYAIAIEDQRLVWREQEAPSSIAADAVRIDVAWAGMNRADAMQRAGHYPPPEGESEIPGL
CEEEEECCCHHEEECCCCCCHHHHCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCE
EVSGTIAEVGRGVERFKPGDKVCALLAGGGYAEQVVVAEAQVLSIPTGLGLREAAALPEV
EECCCHHHHCCCHHHCCCCCCEEEEEECCCCHHHEEEEHHEEEECCCCCCHHHHHHHHHH
FATAWLNLYMEGAVQRGERVVLHAGASGVGTAAIQLCRAFGNPCFVTVGSQAKLEACQRL
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHC
GADAGWNRHDGSFVEPINAWGGADMILDPVGGEYIAQDLQILNAEGRLVVIGPMAGRDAQ
CCCCCCCCCCCCCCCCHHCCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCCCCH
VDLARLLMKRQRIIGSTLRSRSPRAKGAILDALHAHVWPKVTSGEITPLIDRVWPIQEAD
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHH
TAMRHLTDNANTGKILLSVSGEG
HHHHHHCCCCCCCEEEEEECCCC
>Mature Secondary Structure
MYAIAIEDQRLVWREQEAPSSIAADAVRIDVAWAGMNRADAMQRAGHYPPPEGESEIPGL
CEEEEECCCHHEEECCCCCCHHHHCEEEEEEEECCCCHHHHHHHCCCCCCCCCCCCCCCE
EVSGTIAEVGRGVERFKPGDKVCALLAGGGYAEQVVVAEAQVLSIPTGLGLREAAALPEV
EECCCHHHHCCCHHHCCCCCCEEEEEECCCCHHHEEEEHHEEEECCCCCCHHHHHHHHHH
FATAWLNLYMEGAVQRGERVVLHAGASGVGTAAIQLCRAFGNPCFVTVGSQAKLEACQRL
HHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHC
GADAGWNRHDGSFVEPINAWGGADMILDPVGGEYIAQDLQILNAEGRLVVIGPMAGRDAQ
CCCCCCCCCCCCCCCCHHCCCCCCEEEECCCHHHHHHHHHHCCCCCCEEEECCCCCCCCH
VDLARLLMKRQRIIGSTLRSRSPRAKGAILDALHAHVWPKVTSGEITPLIDRVWPIQEAD
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCHHHH
TAMRHLTDNANTGKILLSVSGEG
HHHHHHCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]