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The map label for this gene is tcmK [H]

Identifier: 92115050

GI number: 92115050

Start: 3271907

End: 3273847

Strand: Direct

Name: tcmK [H]

Synonym: Csal_2934

Alternate gene names: 92115050

Gene position: 3271907-3273847 (Clockwise)

Preceding gene: 92115048

Following gene: 92115051

Centisome position: 88.51

GC content: 67.49

Gene sequence:

>1941_bases
ATGACGCATCGTCATCCACAGCATTACGAGGAACGCGGCTTGTCACAGTTACCGGTGATCGTCGGCATGGGCGGTGTTAA
CGCCGCCGGCAGAACCTCGGGCCACCAGGCTTTTCGCCGAACGATTCTCGACGCCTTGCCCGACACCCTTCAGGCACAGA
CCATTCTCGCCCTGGCCGCCATGATGCAGCTTGTGACGCGGCTGGAAGACGGCGCCTGGCAGGATGCCCACGGAGAGATT
CTCGACGCCGCCGACGTCGTTTCGCGCTATCGGCAACACGTACTCGACCATACCCTGATCCGCCGCATCGAAGACGAGCG
TTTCGGCAGCGAAGGCATTCCCGCCAATCGTGCCGCGCAGCTCGACCTCGACGATGCGCTGACCTTTCGCGTGCGCCGGC
GGCAATTGCCCAACGACCTGCCGCCGACCTGGCAGGTGCGCGACATCGACAAGCGCATGGTCGAAGTCGTGGTGCCGCCG
GGGTCGCTGAACGTCCTGCTGCCCGAACGGCGCAGCGCGCAGGTGCGCTCGGCGGGCCAGTTGCCCAGCGGATTCGCGCC
GGAGACCCTGTACCGGAGCGTGCATCACCCGCGCGGGCTGTCGATGACCATCTTCGGCGCCTCCGACTGCCTCGGCCAGA
GCGGCCTGGACTGGTCGGCGCTGCGCAATCGGCTCGACCCGGACGCCATCGCCGTGTATGCCGGCAACTCCATCGGCCAG
CTCGACAACGAGGGCTGGGGCGGCCTGCTGCAGAGCTTCGTCGGCGGCGGACGCGCCACGTCCAAGCAGATGCCGCTGGG
TTACGGCCAGATGCCCGCCGACTTTCTCAACGCCTACGTGCTGGGAAGCGTCGGCGCCACCGGTGCCGTGCTCGGCGCCT
GCGCCAGTTTCCTCTATAACCTGCGCCTGGGGCTGGAGGACATTCGCAGCGGGCGGCGGCGCGTGGTCATGGTGGGCACC
AGCGATGCCCCCATCACGCCGGAAATCGTCGAGGGCTTTCGTACCATGGGCGCGCTGGCCGACGACGAGAGCCTCAAGGC
GCTCGATGCGCTGACGCTGCTGACCGATAGCGACTATCAGCGGGCCTGCCGGCCCTTCGCGCGCAACTGTGGCTTCACCA
TCGCCGAGTCGACCCAGTTCCTGCTGCTGATGGACGATGCCCTGGCGCTCGAGACCGGCGCCCAGATTCTCGGCGCGGTG
CCCGATGTGTTCGTCAACGCCGATGGCTGGAAACGCTCGATCTCCGCGCCGGGCATCGGCAACTACATCACCCTGGCCAA
GTCGGTGAGCCTGGCCCGGGACATGCTCGGCGAGGATTCGGTGCGCCACCGCAGCTACCTGCATGCTCACGGCACCAGCA
CGCCCAAGAATCGCACCACCGAGTCGCACGTGTTCGACATGGTGGCCCGCACGCATGGCATCGAGGCCTGGCCGGTGGCC
GCCATCAAGGCCTTCGTCGGCCATTCGCAAGGCGGCGCGGCGGGCGACCAGCTCGCCAGTGCCCTGGGCACCTTCGCCCA
TGGCGTGATTCCCGGCATCGCCACCCTCGACGCCGTGGCCGACGACGTTCACGCCGAACGTCTGCGTTTCTTTCGCGATC
CGCTCCCTTTCGAGGCCGACGCGACCTTCATCAACGCCAAGGGCTTCGGCGGCAACAACGCTACCGGGCTGGTCATCTCG
CCCGCCGCGACCGAGCGCCTGCTCGCCAAGCGTCACGGTCAGCGCGCCGTCGATGACTGGAAGCATCGTCGCGAGGCCAC
GCTGGCGGCGTCCCAGACGTACCTGGAGACGGCCGACAGCGGCCGCTTCGAGGTCACCTATCGCTTCGGCGAGGGCGTGC
TGGAAGGCCCCGAACTGTCAATGACACGCGACGCCCTGCACATCCCCGGCTTCGATCGGCCGGTATCGCTGACGGTCGAC
AACCCCTACGGTCAGCTGTGA

Upstream 100 bases:

>100_bases
GCACCCGGGTGCTATCATCACCCCAGACTTATTGAGGCTCCGCGCGTCGCCTGACGCGGGGAGACGCGCCATATCGGCCG
ACGCGTGCCGGCCACGAATC

Downstream 100 bases:

>100_bases
AGGCGGCTCCGGGCGTCGACATTGTCGCCGCCCGGCGCCAACCACTACCCTGATGGTGTCTCGGCACAGGATGCATCCAT
GAATATCGTGGTCTATCCCG

Product: beta-ketoacyl synthase

Products: L-tryptophan [Cytoplasm]; L-tyrosine [Cytoplasm]; Proton [Cytoplasm]; L-phenylalanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 646; Mature: 645

Protein sequence:

>646_residues
MTHRHPQHYEERGLSQLPVIVGMGGVNAAGRTSGHQAFRRTILDALPDTLQAQTILALAAMMQLVTRLEDGAWQDAHGEI
LDAADVVSRYRQHVLDHTLIRRIEDERFGSEGIPANRAAQLDLDDALTFRVRRRQLPNDLPPTWQVRDIDKRMVEVVVPP
GSLNVLLPERRSAQVRSAGQLPSGFAPETLYRSVHHPRGLSMTIFGASDCLGQSGLDWSALRNRLDPDAIAVYAGNSIGQ
LDNEGWGGLLQSFVGGGRATSKQMPLGYGQMPADFLNAYVLGSVGATGAVLGACASFLYNLRLGLEDIRSGRRRVVMVGT
SDAPITPEIVEGFRTMGALADDESLKALDALTLLTDSDYQRACRPFARNCGFTIAESTQFLLLMDDALALETGAQILGAV
PDVFVNADGWKRSISAPGIGNYITLAKSVSLARDMLGEDSVRHRSYLHAHGTSTPKNRTTESHVFDMVARTHGIEAWPVA
AIKAFVGHSQGGAAGDQLASALGTFAHGVIPGIATLDAVADDVHAERLRFFRDPLPFEADATFINAKGFGGNNATGLVIS
PAATERLLAKRHGQRAVDDWKHRREATLAASQTYLETADSGRFEVTYRFGEGVLEGPELSMTRDALHIPGFDRPVSLTVD
NPYGQL

Sequences:

>Translated_646_residues
MTHRHPQHYEERGLSQLPVIVGMGGVNAAGRTSGHQAFRRTILDALPDTLQAQTILALAAMMQLVTRLEDGAWQDAHGEI
LDAADVVSRYRQHVLDHTLIRRIEDERFGSEGIPANRAAQLDLDDALTFRVRRRQLPNDLPPTWQVRDIDKRMVEVVVPP
GSLNVLLPERRSAQVRSAGQLPSGFAPETLYRSVHHPRGLSMTIFGASDCLGQSGLDWSALRNRLDPDAIAVYAGNSIGQ
LDNEGWGGLLQSFVGGGRATSKQMPLGYGQMPADFLNAYVLGSVGATGAVLGACASFLYNLRLGLEDIRSGRRRVVMVGT
SDAPITPEIVEGFRTMGALADDESLKALDALTLLTDSDYQRACRPFARNCGFTIAESTQFLLLMDDALALETGAQILGAV
PDVFVNADGWKRSISAPGIGNYITLAKSVSLARDMLGEDSVRHRSYLHAHGTSTPKNRTTESHVFDMVARTHGIEAWPVA
AIKAFVGHSQGGAAGDQLASALGTFAHGVIPGIATLDAVADDVHAERLRFFRDPLPFEADATFINAKGFGGNNATGLVIS
PAATERLLAKRHGQRAVDDWKHRREATLAASQTYLETADSGRFEVTYRFGEGVLEGPELSMTRDALHIPGFDRPVSLTVD
NPYGQL
>Mature_645_residues
THRHPQHYEERGLSQLPVIVGMGGVNAAGRTSGHQAFRRTILDALPDTLQAQTILALAAMMQLVTRLEDGAWQDAHGEIL
DAADVVSRYRQHVLDHTLIRRIEDERFGSEGIPANRAAQLDLDDALTFRVRRRQLPNDLPPTWQVRDIDKRMVEVVVPPG
SLNVLLPERRSAQVRSAGQLPSGFAPETLYRSVHHPRGLSMTIFGASDCLGQSGLDWSALRNRLDPDAIAVYAGNSIGQL
DNEGWGGLLQSFVGGGRATSKQMPLGYGQMPADFLNAYVLGSVGATGAVLGACASFLYNLRLGLEDIRSGRRRVVMVGTS
DAPITPEIVEGFRTMGALADDESLKALDALTLLTDSDYQRACRPFARNCGFTIAESTQFLLLMDDALALETGAQILGAVP
DVFVNADGWKRSISAPGIGNYITLAKSVSLARDMLGEDSVRHRSYLHAHGTSTPKNRTTESHVFDMVARTHGIEAWPVAA
IKAFVGHSQGGAAGDQLASALGTFAHGVIPGIATLDAVADDVHAERLRFFRDPLPFEADATFINAKGFGGNNATGLVISP
AATERLLAKRHGQRAVDDWKHRREATLAASQTYLETADSGRFEVTYRFGEGVLEGPELSMTRDALHIPGFDRPVSLTVDN
PYGQL

Specific function: Catalyzes The Condensation Reaction Of Fatty Acid Synthesis By The Addition To An Acyl Acceptor Of Two Carbons From Malonyl-Acp. Specific For Elongation From C-10 To Unsaturated C-16 And C-18 Fatty Acids. [C]

COG id: COG0304

COG function: function code IQ; 3-oxoacyl-(acyl-carrier-protein) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-ketoacyl-ACP synthases family [H]

Homologues:

None

Paralogues:

None

Copy number: 24024 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 30,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000794
- InterPro:   IPR018201
- InterPro:   IPR014031
- InterPro:   IPR014030
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF00109 ketoacyl-synt; PF02801 Ketoacyl-synt_C [H]

EC number: 2.3.1.41 [C]

Molecular weight: Translated: 69937; Mature: 69806

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHRHPQHYEERGLSQLPVIVGMGGVNAAGRTSGHQAFRRTILDALPDTLQAQTILALAA
CCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MMQLVTRLEDGAWQDAHGEILDAADVVSRYRQHVLDHTLIRRIEDERFGSEGIPANRAAQ
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
LDLDDALTFRVRRRQLPNDLPPTWQVRDIDKRMVEVVVPPGSLNVLLPERRSAQVRSAGQ
CCCHHHHHHHHHHHCCCCCCCCCCEEHHHHHHEEEEEECCCCCEEECCCCCCHHHHHCCC
LPSGFAPETLYRSVHHPRGLSMTIFGASDCLGQSGLDWSALRNRLDPDAIAVYAGNSIGQ
CCCCCCHHHHHHHHCCCCCCEEEEECCHHHCCCCCCCHHHHHCCCCCCEEEEEECCCCCC
LDNEGWGGLLQSFVGGGRATSKQMPLGYGQMPADFLNAYVLGSVGATGAVLGACASFLYN
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LRLGLEDIRSGRRRVVMVGTSDAPITPEIVEGFRTMGALADDESLKALDALTLLTDSDYQ
HHCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH
RACRPFARNCGFTIAESTQFLLLMDDALALETGAQILGAVPDVFVNADGWKRSISAPGIG
HHHHHHHHHCCCEEECCCEEEEEECCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
NYITLAKSVSLARDMLGEDSVRHRSYLHAHGTSTPKNRTTESHVFDMVARTHGIEAWPVA
CHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
AIKAFVGHSQGGAAGDQLASALGTFAHGVIPGIATLDAVADDVHAERLRFFRDPLPFEAD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ATFINAKGFGGNNATGLVISPAATERLLAKRHGQRAVDDWKHRREATLAASQTYLETADS
CCEEECCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GRFEVTYRFGEGVLEGPELSMTRDALHIPGFDRPVSLTVDNPYGQL
CCEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
THRHPQHYEERGLSQLPVIVGMGGVNAAGRTSGHQAFRRTILDALPDTLQAQTILALAA
CCCCCCHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MMQLVTRLEDGAWQDAHGEILDAADVVSRYRQHVLDHTLIRRIEDERFGSEGIPANRAAQ
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
LDLDDALTFRVRRRQLPNDLPPTWQVRDIDKRMVEVVVPPGSLNVLLPERRSAQVRSAGQ
CCCHHHHHHHHHHHCCCCCCCCCCEEHHHHHHEEEEEECCCCCEEECCCCCCHHHHHCCC
LPSGFAPETLYRSVHHPRGLSMTIFGASDCLGQSGLDWSALRNRLDPDAIAVYAGNSIGQ
CCCCCCHHHHHHHHCCCCCCEEEEECCHHHCCCCCCCHHHHHCCCCCCEEEEEECCCCCC
LDNEGWGGLLQSFVGGGRATSKQMPLGYGQMPADFLNAYVLGSVGATGAVLGACASFLYN
CCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
LRLGLEDIRSGRRRVVMVGTSDAPITPEIVEGFRTMGALADDESLKALDALTLLTDSDYQ
HHCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCHH
RACRPFARNCGFTIAESTQFLLLMDDALALETGAQILGAVPDVFVNADGWKRSISAPGIG
HHHHHHHHHCCCEEECCCEEEEEECCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCC
NYITLAKSVSLARDMLGEDSVRHRSYLHAHGTSTPKNRTTESHVFDMVARTHGIEAWPVA
CHHHHHHHHHHHHHHHCCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCCHHHHH
AIKAFVGHSQGGAAGDQLASALGTFAHGVIPGIATLDAVADDVHAERLRFFRDPLPFEAD
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
ATFINAKGFGGNNATGLVISPAATERLLAKRHGQRAVDDWKHRREATLAASQTYLETADS
CCEEECCCCCCCCCCEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
GRFEVTYRFGEGVLEGPELSMTRDALHIPGFDRPVSLTVDNPYGQL
CCEEEEEEECCCCCCCCCCCCCCCCEECCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; DTT; EDTA [C]

Metal ions: Phosphate [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.138 {cis-9-hexadecenoyl-ACP}} 0.017 {cis-9-hexadecenoyl-ACP}} 0.012 {cis-3-decenoyl-ACP}} 0.04 {acetyl-ACP}} 0.031 {acetyl-ACP}} [C]

Substrates: L-tryptophan [Periplasm]; L-tyrosine [Periplasm]; Proton [Periplasm]; L-phenylalanine [Periplasm] [C]

Specific reaction: acyl-ACP + malonyl-ACP = beta-keto-enoyl-ACP + [acyl carrier protein] + CO2 acetyl-ACP + malonyl-ACP = [acyl carrier protein] + acetoacetyl-ACP + CO2 acyl-ACP + malonyl-ACP = [acyl carrier protein] + beta-ketoacyl-ACP + CO2 malonyl-ACP = acetyl-ACP + CO2

General reaction: Acyl group transfer [C]

Inhibitor: Acetyl-CoA; Acyl-CoA; Iodoacetamide; N-Ethyl maleimide acyl -ACP-derivatives protect, malonyl -ACP-decarboxyl ation, [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2684656 [H]