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The map label for this gene is clcD [H]

Identifier: 92114815

GI number: 92114815

Start: 3024431

End: 3025141

Strand: Direct

Name: clcD [H]

Synonym: Csal_2697

Alternate gene names: 92114815

Gene position: 3024431-3025141 (Clockwise)

Preceding gene: 92114812

Following gene: 92114816

Centisome position: 81.82

GC content: 66.53

Gene sequence:

>711_bases
ATGAGCGCATCGCCGGTCACCACGCAGTGGATCGACATTCCGTCGCATGACGGCCAGAGCTTCAAGGGCTATCTGGCCCT
GCCACCCGCGGGCCAGGGGCCCGGTATCGTGCTGATTCAGGAAATCTTCGGCGTCAACGCCCATATCCGCAGCGTCGCCG
AGCAGTACGCACAGGACGGCTACGTGGTCCTCTCCCCGGATGTCTTCTGGCGTCAGGAACCGGGCGTGGAACTCGCCTAC
GAAGGCGCCGACATGGACAAGGCCTTCAAGCTCGTCGGGGACATCGACTTCACGGTGGCCGTCAAGGACCTTGCCAGCAC
CACCCAGGCCTTGCGCCAGCGCGCTGAATGCGACGGCCAGGTCACCGCCATCGGCTACTGCATGGGCGGCATCCTCGCCT
ATCTCGCCGCCATCGAGTCGGGCGTCGACCGCGCGGCAAGCTTCTATCCGGGCGGCATTCACAAGCACCTGGACAAGGCG
TCGCGCCTCGACGTGCCGGTGCAGTTCCATTTCGCAGGCAACGACGGGCACATCCCCGCCGAGACCGTCGACCAGGTCCG
CGCCACCTTCGCCGACCGTGACGACGTCACGATCCACGTCTACGAAGGGGCCGACCACGGCTTCAACTGCTGGGCCCGCG
GTGCCTACCATCGCCACGCCGCGGCGCTCTCCCACGGCCGTGCCCTGGTCTTTCTGGAAGGGCGGGACTGA

Upstream 100 bases:

>100_bases
GGTCTGGCGGCAGGTATCATTTGCCTGCGAACCACCTGGCCTCGCCGACAATGACTCTCTAGACTGGGGCCGAGTGCTTC
CCTCACCTCAAGGAGACGAC

Downstream 100 bases:

>100_bases
TCGCGGCACGCGCCCACGCCCGCGAGGTCGCGACACCGGGCCTCGCCGGCCCATGAGCGGTTACCCTCTGCGCCTACCAT
GGAGCTCCCATGTCATATCG

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSASPVTTQWIDIPSHDGQSFKGYLALPPAGQGPGIVLIQEIFGVNAHIRSVAEQYAQDGYVVLSPDVFWRQEPGVELAY
EGADMDKAFKLVGDIDFTVAVKDLASTTQALRQRAECDGQVTAIGYCMGGILAYLAAIESGVDRAASFYPGGIHKHLDKA
SRLDVPVQFHFAGNDGHIPAETVDQVRATFADRDDVTIHVYEGADHGFNCWARGAYHRHAAALSHGRALVFLEGRD

Sequences:

>Translated_236_residues
MSASPVTTQWIDIPSHDGQSFKGYLALPPAGQGPGIVLIQEIFGVNAHIRSVAEQYAQDGYVVLSPDVFWRQEPGVELAY
EGADMDKAFKLVGDIDFTVAVKDLASTTQALRQRAECDGQVTAIGYCMGGILAYLAAIESGVDRAASFYPGGIHKHLDKA
SRLDVPVQFHFAGNDGHIPAETVDQVRATFADRDDVTIHVYEGADHGFNCWARGAYHRHAAALSHGRALVFLEGRD
>Mature_235_residues
SASPVTTQWIDIPSHDGQSFKGYLALPPAGQGPGIVLIQEIFGVNAHIRSVAEQYAQDGYVVLSPDVFWRQEPGVELAYE
GADMDKAFKLVGDIDFTVAVKDLASTTQALRQRAECDGQVTAIGYCMGGILAYLAAIESGVDRAASFYPGGIHKHLDKAS
RLDVPVQFHFAGNDGHIPAETVDQVRATFADRDDVTIHVYEGADHGFNCWARGAYHRHAAALSHGRALVFLEGRD

Specific function: Ring cleavage of cyclic ester dienelactone to produce maleylacetate [H]

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=235, Percent_Identity=27.6595744680851, Blast_Score=80, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320117, Length=208, Percent_Identity=24.5192307692308, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 25555; Mature: 25424

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASPVTTQWIDIPSHDGQSFKGYLALPPAGQGPGIVLIQEIFGVNAHIRSVAEQYAQDG
CCCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCC
YVVLSPDVFWRQEPGVELAYEGADMDKAFKLVGDIDFTVAVKDLASTTQALRQRAECDGQ
EEEECCCEEECCCCCCEEEECCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCC
VTAIGYCMGGILAYLAAIESGVDRAASFYPGGIHKHLDKASRLDVPVQFHFAGNDGHIPA
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCH
ETVDQVRATFADRDDVTIHVYEGADHGFNCWARGAYHRHAAALSHGRALVFLEGRD
HHHHHHHHHHCCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHCCCCEEEEEECCC
>Mature Secondary Structure 
SASPVTTQWIDIPSHDGQSFKGYLALPPAGQGPGIVLIQEIFGVNAHIRSVAEQYAQDG
CCCCCEEEEEECCCCCCCCCCCEEEECCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHCC
YVVLSPDVFWRQEPGVELAYEGADMDKAFKLVGDIDFTVAVKDLASTTQALRQRAECDGQ
EEEECCCEEECCCCCCEEEECCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCCCC
VTAIGYCMGGILAYLAAIESGVDRAASFYPGGIHKHLDKASRLDVPVQFHFAGNDGHIPA
EEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCH
ETVDQVRATFADRDDVTIHVYEGADHGFNCWARGAYHRHAAALSHGRALVFLEGRD
HHHHHHHHHHCCCCCEEEEEEECCCCCCCHHCCCCHHHHHHHHCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3299368 [H]