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The map label for this gene is nudE [H]

Identifier: 92114779

GI number: 92114779

Start: 2977758

End: 2978393

Strand: Direct

Name: nudE [H]

Synonym: Csal_2661

Alternate gene names: 92114779

Gene position: 2977758-2978393 (Clockwise)

Preceding gene: 92114769

Following gene: 92114792

Centisome position: 80.55

GC content: 65.57

Gene sequence:

>636_bases
ATGGCCTTTTCTTCCCGCTCAGGCGATACCCCGCCGCCCGCGCCCGAGACCGACGGAACGCGTTCCAAGCCACGCATCCT
GGCGCGCCACGAAGTCGCGCGCAGCCGCTTGTTCGCCATCGAATCGCTGGAACTGCGGTTCGCCAACGGTGCCGAACGTA
CCTTCGAACGCCTCATGGGCAGCGGCAACGGTCATGGCGGCGGTGCGGTCATGATCGTGGCCATGCCGGACCCCGACCAC
GTGCTGCTGATCCACGAGTATGCCGCGGGCTTCGACGACTATGCGTTGACCTTGCCCAAGGGCCTCGTCGATCCCGGGGA
AGACGTCGTCAGCGCCGCGAATCGCGAGCTGATGGAAGAATGCGGAGTGGGAGCCCGGCACATCGAGCCCTTGGTGGAGC
TGTCGCTGGCGCCCAACTACATGAGCCACCGCATGCACGTGATGCTGGCCACCGACCTGTACGAAAAGCGCTTGCCCGGC
GACGAACCGGAACCCCTGATCGTCGAGCGCTATCCTCTCGAGGACATCAACGGCCTGCTGGCCCGCGAGGACTTCCACGA
AGGACGTGCCATCGCGGCGCTGTTCATCGCACGCGACCGTATGCGCAGCGAGCGCGTCGACGATGCCTGGTGGTAA

Upstream 100 bases:

>100_bases
ATGGCGTTCCAATCAATCATGCGGGGCTCCGCCAAGATGGATATCGAAGGTCTTGTGCTCTTATTGTACGCTGCATGACA
GAGACCCGCACGGAGACACC

Downstream 100 bases:

>100_bases
GGCAACGGGCCGACACCTGGCGACTGGTCGGTCACGACGAAACGAGGCCCCGACATGTCGGGGCCTCGCGTCATCGCATC
CTGTGATGTGCCGGCTCTAT

Product: ADP-ribose diphosphatase NudE

Products: AMP; D-Ribose 5- Phosphate. [C]

Alternate protein names: NA

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MAFSSRSGDTPPPAPETDGTRSKPRILARHEVARSRLFAIESLELRFANGAERTFERLMGSGNGHGGGAVMIVAMPDPDH
VLLIHEYAAGFDDYALTLPKGLVDPGEDVVSAANRELMEECGVGARHIEPLVELSLAPNYMSHRMHVMLATDLYEKRLPG
DEPEPLIVERYPLEDINGLLAREDFHEGRAIAALFIARDRMRSERVDDAWW

Sequences:

>Translated_211_residues
MAFSSRSGDTPPPAPETDGTRSKPRILARHEVARSRLFAIESLELRFANGAERTFERLMGSGNGHGGGAVMIVAMPDPDH
VLLIHEYAAGFDDYALTLPKGLVDPGEDVVSAANRELMEECGVGARHIEPLVELSLAPNYMSHRMHVMLATDLYEKRLPG
DEPEPLIVERYPLEDINGLLAREDFHEGRAIAALFIARDRMRSERVDDAWW
>Mature_210_residues
AFSSRSGDTPPPAPETDGTRSKPRILARHEVARSRLFAIESLELRFANGAERTFERLMGSGNGHGGGAVMIVAMPDPDHV
LLIHEYAAGFDDYALTLPKGLVDPGEDVVSAANRELMEECGVGARHIEPLVELSLAPNYMSHRMHVMLATDLYEKRLPGD
EPEPLIVERYPLEDINGLLAREDFHEGRAIAALFIARDRMRSERVDDAWW

Specific function: Active on adenosine(5')triphospho(5')adenosine (Ap3A), ADP-ribose, NADH, adenosine(5')diphospho(5')adenosine (Ap2A) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=183, Percent_Identity=48.0874316939891, Blast_Score=166, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 23458; Mature: 23327

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSSRSGDTPPPAPETDGTRSKPRILARHEVARSRLFAIESLELRFANGAERTFERLMG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHC
SGNGHGGGAVMIVAMPDPDHVLLIHEYAAGFDDYALTLPKGLVDPGEDVVSAANRELMEE
CCCCCCCCEEEEEEECCCCCEEEEEHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
CGVGARHIEPLVELSLAPNYMSHRMHVMLATDLYEKRLPGDEPEPLIVERYPLEDINGLL
HCCCHHHHHHHHHHHCCCCHHHHHEEEEEHHHHHHHHCCCCCCCCEEEEECCHHHHHHHH
AREDFHEGRAIAALFIARDRMRSERVDDAWW
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AFSSRSGDTPPPAPETDGTRSKPRILARHEVARSRLFAIESLELRFANGAERTFERLMG
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHC
SGNGHGGGAVMIVAMPDPDHVLLIHEYAAGFDDYALTLPKGLVDPGEDVVSAANRELMEE
CCCCCCCCEEEEEEECCCCCEEEEEHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHH
CGVGARHIEPLVELSLAPNYMSHRMHVMLATDLYEKRLPGDEPEPLIVERYPLEDINGLL
HCCCHHHHHHHHHHHCCCCHHHHHEEEEEHHHHHHHHCCCCCCCCEEEEECCHHHHHHHH
AREDFHEGRAIAALFIARDRMRSERVDDAWW
HHHHHHCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ADP-Ribose; H2O [C]

Specific reaction: ADP-Ribose + H2O = AMP + D-Ribose 5- Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9452430 [H]