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The map label for this gene is rluE [H]

Identifier: 92114560

GI number: 92114560

Start: 2747450

End: 2748223

Strand: Direct

Name: rluE [H]

Synonym: Csal_2442

Alternate gene names: 92114560

Gene position: 2747450-2748223 (Clockwise)

Preceding gene: 92114557

Following gene: 92114561

Centisome position: 74.32

GC content: 69.51

Gene sequence:

>774_bases
GTGAACGCCACCGGCCGCTCCGGTTCTCTCAATGTGCGGCCGGCCGCACGTGGATGCAAGGCGGGCGCCGCGAAAAAACA
CGCGCCCCGGTACCCCGCAAGTATGGCATTGCGCCCCGGGTGCGGAGTAGGCTTTGCCCCATGTGCGCCGCCGTCGACAG
GCCCGAGACCGCCAATGAGTTCGCTGTACCTGCTGCATAAACCTTACCAAATGCTCTCGCAGTTCACCGATACACGGGGA
CGCGCCACGCTTGCCGAGGCCATCGACGTGCCCGACATCTACCCGGCCGGGCGTCTCGACTACGACTCGGAAGGCTTGCT
GCTGCTCACCGGAGACGGTGCACTGGCGCATCGCATCAGTCATCCGCGCCACAAGCAGCCCAAGACCTACTGGGTGCAGG
TCGAGGGCGAGCCGGAGCCCGCCGCATTGCAGGCCCTGCGCCAGGGCGTGACGCTGCGTGACGGCCCCACCCGCCCGGCC
AAGGTACGCCGTCTCGAGTCACCCGATGTCGCGCCACGCCACCCGCCCGTGCGGCATCGCGAGGCCGTGCCGACCGCGTG
GCTCGAGCTCACCCTGAGCGAGGGACGCAATCGCCAGGTTCGCCGCATGACGGCGCATGTCGGCCACCCCACGCTACGCT
TGATTCGTGTCGCCATCGGATCATGGCACCTCGCCGGGCTGGCGCCTGGCGAATGGCGCCGCGAGACCCTGCATGCGCCG
CGCGCCCAGGCCGCGCCGGCTTCCTCCCACAAGCGCCGCCGGAGTCGCCGATGA

Upstream 100 bases:

>100_bases
TCTGCGTAAGACATGGGGGAGACGAATGGGTGTTCTGGTTCAAACATAGATGGAAATGTCCGTAAGTGCGCGCCGGATTC
GACGGGGCAGGGCCACCAGG

Downstream 100 bases:

>100_bases
GCCGCTGGTCTCCCCATGTCACCGTCGCCAGCGTCATCGAGCGCGCGGGCCGTTACCTGCTGGTCGAGGAAGACAAGGGC
GGGCCCTTCAGCCTGTTCAA

Product: ribosomal large subunit pseudouridine synthase E

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MNATGRSGSLNVRPAARGCKAGAAKKHAPRYPASMALRPGCGVGFAPCAPPSTGPRPPMSSLYLLHKPYQMLSQFTDTRG
RATLAEAIDVPDIYPAGRLDYDSEGLLLLTGDGALAHRISHPRHKQPKTYWVQVEGEPEPAALQALRQGVTLRDGPTRPA
KVRRLESPDVAPRHPPVRHREAVPTAWLELTLSEGRNRQVRRMTAHVGHPTLRLIRVAIGSWHLAGLAPGEWRRETLHAP
RAQAAPASSHKRRRSRR

Sequences:

>Translated_257_residues
MNATGRSGSLNVRPAARGCKAGAAKKHAPRYPASMALRPGCGVGFAPCAPPSTGPRPPMSSLYLLHKPYQMLSQFTDTRG
RATLAEAIDVPDIYPAGRLDYDSEGLLLLTGDGALAHRISHPRHKQPKTYWVQVEGEPEPAALQALRQGVTLRDGPTRPA
KVRRLESPDVAPRHPPVRHREAVPTAWLELTLSEGRNRQVRRMTAHVGHPTLRLIRVAIGSWHLAGLAPGEWRRETLHAP
RAQAAPASSHKRRRSRR
>Mature_257_residues
MNATGRSGSLNVRPAARGCKAGAAKKHAPRYPASMALRPGCGVGFAPCAPPSTGPRPPMSSLYLLHKPYQMLSQFTDTRG
RATLAEAIDVPDIYPAGRLDYDSEGLLLLTGDGALAHRISHPRHKQPKTYWVQVEGEPEPAALQALRQGVTLRDGPTRPA
KVRRLESPDVAPRHPPVRHREAVPTAWLELTLSEGRNRQVRRMTAHVGHPTLRLIRVAIGSWHLAGLAPGEWRRETLHAP
RAQAAPASSHKRRRSRR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=178, Percent_Identity=57.8651685393258, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1787524, Length=205, Percent_Identity=33.1707317073171, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788510, Length=186, Percent_Identity=35.4838709677419, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1790453, Length=232, Percent_Identity=26.7241379310345, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 28204; Mature: 28204

Theoretical pI: Translated: 11.75; Mature: 11.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATGRSGSLNVRPAARGCKAGAAKKHAPRYPASMALRPGCGVGFAPCAPPSTGPRPPMS
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCHH
SLYLLHKPYQMLSQFTDTRGRATLAEAIDVPDIYPAGRLDYDSEGLLLLTGDGALAHRIS
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHC
HPRHKQPKTYWVQVEGEPEPAALQALRQGVTLRDGPTRPAKVRRLESPDVAPRHPPVRHR
CCCCCCCCEEEEEEECCCCHHHHHHHHCCCEECCCCCCHHHHHCCCCCCCCCCCCCCCCH
EAVPTAWLELTLSEGRNRQVRRMTAHVGHPTLRLIRVAIGSWHLAGLAPGEWRRETLHAP
HCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHCCC
RAQAAPASSHKRRRSRR
CCCCCCCHHHHHHHCCC
>Mature Secondary Structure
MNATGRSGSLNVRPAARGCKAGAAKKHAPRYPASMALRPGCGVGFAPCAPPSTGPRPPMS
CCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCHH
SLYLLHKPYQMLSQFTDTRGRATLAEAIDVPDIYPAGRLDYDSEGLLLLTGDGALAHRIS
HHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCHHHHHHC
HPRHKQPKTYWVQVEGEPEPAALQALRQGVTLRDGPTRPAKVRRLESPDVAPRHPPVRHR
CCCCCCCCEEEEEEECCCCHHHHHHHHCCCEECCCCCCHHHHHCCCCCCCCCCCCCCCCH
EAVPTAWLELTLSEGRNRQVRRMTAHVGHPTLRLIRVAIGSWHLAGLAPGEWRRETLHAP
HCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCEEECCCCCCHHHHHHHCCC
RAQAAPASSHKRRRSRR
CCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]