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The map label for this gene is iciA [H]

Identifier: 92114551

GI number: 92114551

Start: 2736803

End: 2737711

Strand: Direct

Name: iciA [H]

Synonym: Csal_2433

Alternate gene names: 92114551

Gene position: 2736803-2737711 (Clockwise)

Preceding gene: 92114547

Following gene: 92114555

Centisome position: 74.03

GC content: 66.78

Gene sequence:

>909_bases
ATGCTCGACTACAAACTTCTCGAAGCACTGGCCGTCGTGGTCGATCAGGGGGGATTCGAACGCGGCGCGTATTACCTGGG
GCTCACTCAGTCGGCCGTCTCGCAGCGCATCAAATTGCTCGAAGCTCGCCTGGGGCAACCGGTGCTGGTACGCACGCCCA
AGCTCGCCCCGACGCCCCTGGGGCGCCGGCTGCTCAACCACGTTCAGCAGGTACGCTTGCTCGAACATGACTTGCTCGAT
CAGATCCCTGCCCTCGGCGAGCGCGAACAGCGGCTGCGTCTGGCCATCAACGCCGATAGCCTGGCCACGTGGTGGTCGCC
GACGACCGGCGAGTTCTGCCGTCGCCAAGGGGTGCTGCTGGACTTGATCGTCGAGGATCAGGACGTGGCGCTCAAGCGCA
TGCGCGACGGCGAGGTGGCCGGCTGCATCTGCGCCACACCGCGCCCCGTGCAAGGCGCCCGCGCGCATGCCCTGGGCAGC
ATGCGCTACCGTGCCATCGCCAGCCCCAGCTACGTCTCACGTCATTTCCATGACGGCTTGACGCACGATGCCTTGCGCCA
GGCGCCGGCCATCGTGTTCGGCCCCGACGACCGCCTCCAGCATCGCTACCTGGAGATGCGCGGCTACAAGGAATCCTTTC
CCCACCACCTTTGCCCTTCCTCGGAAGGCTTCGTGCATCTTGCCCTGGCCGGCATGGGCTATGGCCTGATTCCCGAGCGC
CAGGTGGCGCGCGAGCTGGAGGAAGGCCGCCTGGTGGACCTCGATCCGGACAGTCACATCGACGTGCCCCTTTACTGGCA
CCACTGGCGGCACGGCGGCGACACCCTGGATGCCTTGACGGCCCATCTGCTCGACATGAGCAGTGCCTGGCTCACGCCGC
CGGCGCCGACGGCGGCGTCGCTGACATAG

Upstream 100 bases:

>100_bases
TTCTCCCTTTCAGCCGGGATACGTTGATAAGTGGCGCAGAATACGCTTGACTACAGCAATAAGGTAAATTAATTCAAGGA
ATGGGTGATAACGTTTACTG

Downstream 100 bases:

>100_bases
CACGCGTCGCCGGGTCAGCGATCAGGCGTCGCCCAACCGGCGCGCCACGCTGGCCACCTTGTCGTCCATGTGCTGTTCGC
GATCGGTCCGCGTGCCCATC

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MLDYKLLEALAVVVDQGGFERGAYYLGLTQSAVSQRIKLLEARLGQPVLVRTPKLAPTPLGRRLLNHVQQVRLLEHDLLD
QIPALGEREQRLRLAINADSLATWWSPTTGEFCRRQGVLLDLIVEDQDVALKRMRDGEVAGCICATPRPVQGARAHALGS
MRYRAIASPSYVSRHFHDGLTHDALRQAPAIVFGPDDRLQHRYLEMRGYKESFPHHLCPSSEGFVHLALAGMGYGLIPER
QVARELEEGRLVDLDPDSHIDVPLYWHHWRHGGDTLDALTAHLLDMSSAWLTPPAPTAASLT

Sequences:

>Translated_302_residues
MLDYKLLEALAVVVDQGGFERGAYYLGLTQSAVSQRIKLLEARLGQPVLVRTPKLAPTPLGRRLLNHVQQVRLLEHDLLD
QIPALGEREQRLRLAINADSLATWWSPTTGEFCRRQGVLLDLIVEDQDVALKRMRDGEVAGCICATPRPVQGARAHALGS
MRYRAIASPSYVSRHFHDGLTHDALRQAPAIVFGPDDRLQHRYLEMRGYKESFPHHLCPSSEGFVHLALAGMGYGLIPER
QVARELEEGRLVDLDPDSHIDVPLYWHHWRHGGDTLDALTAHLLDMSSAWLTPPAPTAASLT
>Mature_302_residues
MLDYKLLEALAVVVDQGGFERGAYYLGLTQSAVSQRIKLLEARLGQPVLVRTPKLAPTPLGRRLLNHVQQVRLLEHDLLD
QIPALGEREQRLRLAINADSLATWWSPTTGEFCRRQGVLLDLIVEDQDVALKRMRDGEVAGCICATPRPVQGARAHALGS
MRYRAIASPSYVSRHFHDGLTHDALRQAPAIVFGPDDRLQHRYLEMRGYKESFPHHLCPSSEGFVHLALAGMGYGLIPER
QVARELEEGRLVDLDPDSHIDVPLYWHHWRHGGDTLDALTAHLLDMSSAWLTPPAPTAASLT

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=285, Percent_Identity=38.2456140350877, Blast_Score=175, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33710; Mature: 33710

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYKLLEALAVVVDQGGFERGAYYLGLTQSAVSQRIKLLEARLGQPVLVRTPKLAPTPL
CCHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCHH
GRRLLNHVQQVRLLEHDLLDQIPALGEREQRLRLAINADSLATWWSPTTGEFCRRQGVLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHCCCEE
DLIVEDQDVALKRMRDGEVAGCICATPRPVQGARAHALGSMRYRAIASPSYVSRHFHDGL
EEEECCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCC
THDALRQAPAIVFGPDDRLQHRYLEMRGYKESFPHHLCPSSEGFVHLALAGMGYGLIPER
CHHHHHHCCEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEECCCCCCCCHH
QVARELEEGRLVDLDPDSHIDVPLYWHHWRHGGDTLDALTAHLLDMSSAWLTPPAPTAAS
HHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
LT
CC
>Mature Secondary Structure
MLDYKLLEALAVVVDQGGFERGAYYLGLTQSAVSQRIKLLEARLGQPVLVRTPKLAPTPL
CCHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHHHHHHCCCCEEEECCCCCCCHH
GRRLLNHVQQVRLLEHDLLDQIPALGEREQRLRLAINADSLATWWSPTTGEFCRRQGVLL
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHCCCEE
DLIVEDQDVALKRMRDGEVAGCICATPRPVQGARAHALGSMRYRAIASPSYVSRHFHDGL
EEEECCHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCC
THDALRQAPAIVFGPDDRLQHRYLEMRGYKESFPHHLCPSSEGFVHLALAGMGYGLIPER
CHHHHHHCCEEEECCCHHHHHHHHHHCCCHHHCCCCCCCCCCCEEEEEEECCCCCCCCHH
QVARELEEGRLVDLDPDSHIDVPLYWHHWRHGGDTLDALTAHLLDMSSAWLTPPAPTAAS
HHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
LT
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA