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The map label for this gene is miaB [H]

Identifier: 92114455

GI number: 92114455

Start: 2623851

End: 2625230

Strand: Direct

Name: miaB [H]

Synonym: Csal_2334

Alternate gene names: 92114455

Gene position: 2623851-2625230 (Clockwise)

Preceding gene: 92114452

Following gene: 92114456

Centisome position: 70.98

GC content: 63.62

Gene sequence:

>1380_bases
ATGTCGGGCGCTCGACGCACCGACCGTATCGGTATGGCGAAGAAACTCTTCATCAAGACCCATGGCTGCCAGATGAACGA
GTACGATTCCGCGCGCATGGCGGATCTACTCGGTGAATCGCACGCACTCGAGCTGACCGACGACGAGCGGGACGCCGACG
TCATCCTGCTCAACACCTGCTCGATTCGCGAAAAGGCCCAAGAGAAGGTGTTCCACCAGCTCGGTCGCTGGAAGAAGCTC
AAGGCCGCCAATCCCGACCTCGTGATCGGCGTCGGGGGCTGCGTGGCCAGCCAGGAAGGCGAGCACATCAAGAAGCGCGC
GCCGCACGTCGACATGGTCTTCGGTCCGCAGACACTGCACCGCCTGCCGACCATGCTCGACTCGCGTCAGGGCGCGGAGA
CCATTTCCATGGTCGACGTCACCTTCCCCGAGATCGAGAAGTTCGACCACCTGCCCAAGCCCACGTCGGACGGCGCCACG
GCCTTCGTCTCGGTGATGGAAGGCTGCTCGAAATACTGCACCTTCTGCGTGGTGCCTTATACCCGTGGCGAGGAAGTCTC
GCGGCCCTTCGAGTCGGTGATGGAGGAAGTCATCCATCTGGCCGACCAGGGCGTGCGCGAGATCAACCTGCTGGGCCAGA
ACGTCAACGCCTATCGCGGCGAGAATCAGCTCGGCGACGAGATCGACCTCGCCGAGCTGATCGGCTGCGTCGCCGCCGTG
GAAGGCATCGACCGGATCCGTTTCACCACCTCGCACCCCGTGGAATTTTCCGACAGCCTGATCGAGGCCTACGGCGAGAT
TCCCGAGCTGGTCAGCCATCTGCACCTGCCCGTTCAGGCGGGGTCGGACCGTATCCTGGCGGCCATGAAGCGCGGTCACA
CCGTCGAGGAATACGTCGACAAGCTGGAGCGTATCCGCGCCCTGCGCCCGGACATCAGCTTCTCCTCGGACTTCATCATC
GGCTTCCCCGGCGAGACCGAGGAAGACTTCATGGACACCATGAACCTCATCCAGCGCATCGGATTCGACGCCTCGTTCAG
CTTCGTCTACTCCCCGCGCCCTGGGACGCCGGCGGCGAACCTGGAAGACGAGACGCCGGAAGCGACCAAGAAGCAGCGTC
TGGCGATTCTCCAGGAGCGTCTCAACCAGCAGACCATGCAGATCTCGCGGCGCATGGTCGGCAACACCGAGCGCATTCTG
GTCACCGGCTTCTCGCCCAAGGATCCCGGCCAGCTTTCCGGCCGCACCGAGAACAACCGGGTGGTGAATTTCCGCGCGCC
CAACCCCACCGAACTCATCGGCTATTTCGTCGACGTCGAGATCACCGAGGCCTTGCCGAACTCGCTGCGCGGTGACCTGG
CGTCGCCCGCGCGCTACTGA

Upstream 100 bases:

>100_bases
CCCAGGCTGCCGGCAATCCGTATAATGCAATGTCTTATCCTGGACAAGCGGCTGGGCCGCATTTCAAGGCCACGCTTTTA
CTCACCCCCAATCACGGACA

Downstream 100 bases:

>100_bases
CGTGCCATTCACCGCTCGGTGAAACCTTGCGCAAGATCATTGCCCCGGCGTTGTCCGGGGCGCTAAGCTGAATTCACACT
TCCAACCCATGGATCCCCTC

Product: tRNA-i(6)A37 modification protein MiaB

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSGARRTDRIGMAKKLFIKTHGCQMNEYDSARMADLLGESHALELTDDERDADVILLNTCSIREKAQEKVFHQLGRWKKL
KAANPDLVIGVGGCVASQEGEHIKKRAPHVDMVFGPQTLHRLPTMLDSRQGAETISMVDVTFPEIEKFDHLPKPTSDGAT
AFVSVMEGCSKYCTFCVVPYTRGEEVSRPFESVMEEVIHLADQGVREINLLGQNVNAYRGENQLGDEIDLAELIGCVAAV
EGIDRIRFTTSHPVEFSDSLIEAYGEIPELVSHLHLPVQAGSDRILAAMKRGHTVEEYVDKLERIRALRPDISFSSDFII
GFPGETEEDFMDTMNLIQRIGFDASFSFVYSPRPGTPAANLEDETPEATKKQRLAILQERLNQQTMQISRRMVGNTERIL
VTGFSPKDPGQLSGRTENNRVVNFRAPNPTELIGYFVDVEITEALPNSLRGDLASPARY

Sequences:

>Translated_459_residues
MSGARRTDRIGMAKKLFIKTHGCQMNEYDSARMADLLGESHALELTDDERDADVILLNTCSIREKAQEKVFHQLGRWKKL
KAANPDLVIGVGGCVASQEGEHIKKRAPHVDMVFGPQTLHRLPTMLDSRQGAETISMVDVTFPEIEKFDHLPKPTSDGAT
AFVSVMEGCSKYCTFCVVPYTRGEEVSRPFESVMEEVIHLADQGVREINLLGQNVNAYRGENQLGDEIDLAELIGCVAAV
EGIDRIRFTTSHPVEFSDSLIEAYGEIPELVSHLHLPVQAGSDRILAAMKRGHTVEEYVDKLERIRALRPDISFSSDFII
GFPGETEEDFMDTMNLIQRIGFDASFSFVYSPRPGTPAANLEDETPEATKKQRLAILQERLNQQTMQISRRMVGNTERIL
VTGFSPKDPGQLSGRTENNRVVNFRAPNPTELIGYFVDVEITEALPNSLRGDLASPARY
>Mature_458_residues
SGARRTDRIGMAKKLFIKTHGCQMNEYDSARMADLLGESHALELTDDERDADVILLNTCSIREKAQEKVFHQLGRWKKLK
AANPDLVIGVGGCVASQEGEHIKKRAPHVDMVFGPQTLHRLPTMLDSRQGAETISMVDVTFPEIEKFDHLPKPTSDGATA
FVSVMEGCSKYCTFCVVPYTRGEEVSRPFESVMEEVIHLADQGVREINLLGQNVNAYRGENQLGDEIDLAELIGCVAAVE
GIDRIRFTTSHPVEFSDSLIEAYGEIPELVSHLHLPVQAGSDRILAAMKRGHTVEEYVDKLERIRALRPDISFSSDFIIG
FPGETEEDFMDTMNLIQRIGFDASFSFVYSPRPGTPAANLEDETPEATKKQRLAILQERLNQQTMQISRRMVGNTERILV
TGFSPKDPGQLSGRTENNRVVNFRAPNPTELIGYFVDVEITEALPNSLRGDLASPARY

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=483, Percent_Identity=37.2670807453416, Blast_Score=296, Evalue=4e-80,
Organism=Homo sapiens, GI28872782, Length=483, Percent_Identity=37.2670807453416, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI93277076, Length=463, Percent_Identity=26.7818574514039, Blast_Score=156, Evalue=5e-38,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=66.1399548532731, Blast_Score=611, Evalue=1e-176,
Organism=Escherichia coli, GI1787057, Length=386, Percent_Identity=28.4974093264249, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=471, Percent_Identity=32.2717622080679, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=310, Percent_Identity=30.3225806451613, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI21356207, Length=437, Percent_Identity=34.7826086956522, Blast_Score=265, Evalue=7e-71,
Organism=Drosophila melanogaster, GI19922432, Length=468, Percent_Identity=28.8461538461538, Blast_Score=145, Evalue=7e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 51185; Mature: 51053

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGARRTDRIGMAKKLFIKTHGCQMNEYDSARMADLLGESHALELTDDERDADVILLNTC
CCCCCCCHHHCHHHHHEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCEEEEECC
SIREKAQEKVFHQLGRWKKLKAANPDLVIGVGGCVASQEGEHIKKRAPHVDMVFGPQTLH
HHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCHHCCCCCHHHHHHCCCCEEEECHHHHH
RLPTMLDSRQGAETISMVDVTFPEIEKFDHLPKPTSDGATAFVSVMEGCSKYCTFCVVPY
HHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEC
TRGEEVSRPFESVMEEVIHLADQGVREINLLGQNVNAYRGENQLGDEIDLAELIGCVAAV
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHH
EGIDRIRFTTSHPVEFSDSLIEAYGEIPELVSHLHLPVQAGSDRILAAMKRGHTVEEYVD
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
KLERIRALRPDISFSSDFIIGFPGETEEDFMDTMNLIQRIGFDASFSFVYSPRPGTPAAN
HHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
LEDETPEATKKQRLAILQERLNQQTMQISRRMVGNTERILVTGFSPKDPGQLSGRTENNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCE
VVNFRAPNPTELIGYFVDVEITEALPNSLRGDLASPARY
EEEEECCCHHHHEEEEEEEEEHHHCCHHHCCCCCCCCCC
>Mature Secondary Structure 
SGARRTDRIGMAKKLFIKTHGCQMNEYDSARMADLLGESHALELTDDERDADVILLNTC
CCCCCCHHHCHHHHHEEEECCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCEEEEECC
SIREKAQEKVFHQLGRWKKLKAANPDLVIGVGGCVASQEGEHIKKRAPHVDMVFGPQTLH
HHHHHHHHHHHHHHCCCHHHCCCCCCEEEECCCHHCCCCCHHHHHHCCCCEEEECHHHHH
RLPTMLDSRQGAETISMVDVTFPEIEKFDHLPKPTSDGATAFVSVMEGCSKYCTFCVVPY
HHHHHHHCCCCCCEEEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEEEEC
TRGEEVSRPFESVMEEVIHLADQGVREINLLGQNVNAYRGENQLGDEIDLAELIGCVAAV
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHCCCCCCCCCCCHHHHHHHHHHH
EGIDRIRFTTSHPVEFSDSLIEAYGEIPELVSHLHLPVQAGSDRILAAMKRGHTVEEYVD
HHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
KLERIRALRPDISFSSDFIIGFPGETEEDFMDTMNLIQRIGFDASFSFVYSPRPGTPAAN
HHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCC
LEDETPEATKKQRLAILQERLNQQTMQISRRMVGNTERILVTGFSPKDPGQLSGRTENNR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCE
VVNFRAPNPTELIGYFVDVEITEALPNSLRGDLASPARY
EEEEECCCHHHHEEEEEEEEEHHHCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA