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The map label for this gene is metG

Identifier: 92114242

GI number: 92114242

Start: 2388920

End: 2390965

Strand: Direct

Name: metG

Synonym: Csal_2120

Alternate gene names: 92114242

Gene position: 2388920-2390965 (Clockwise)

Preceding gene: 92114241

Following gene: 92114243

Centisome position: 64.62

GC content: 64.17

Gene sequence:

>2046_bases
ATGCCGACTACCGCCAACGCCAAGCGCAAGATCCTGGTCACCAGTGCCCTGCCTTATGCCAACGGCTCGATCCATCTGGG
CCACCTGCTCGAGTACATCCAGACCGATATCTGGGTGCGCTTCCAGAAGAGCCGCGGCCACGAGTGTCATTACGTGTGTG
CGGATGACGCCCATGGCACCGCGATCATGCTGCGCGCGGAGCAAGAAGGCATCACTCCGGAACAACTGATCAGCAAGGTC
AGCGCCGAACACCAGGCCGACTTCGCCCGCTTCGGGGTGGCATTCGACAATTACTATTCCACCCACTCGGAAGAGAACCG
TCAGCACAGCGAGCGCATCTACACGGCGCTGCGTGACGCCGGCCATATCTCGACGCGTGACATCGAGCAGATGTACGACC
CGGTGAAGGGCCTGTTCCTCGCCGACCGTTTCATCAAGGGCACCTGCCCCAAATGCGGGGCGGGCGATCAGTACGGCGAC
AATTGCGAGGCGTGCGGAGCCACCTATACGCCGGCCGAGCTGATCGATCCGGTCTCGGCGATCAGCGGTGCCACGCCCGA
GGTGCGCAGCTCGACGCACTTCTTCTTCAAGCTGCCCGACTTCGCCGACTTCATGCAGGGCTGGATCGACGGCGGCCACG
TCCAGCCGCAGATCCGCAACAAGTTGCTCGAATGGTTCGAATCGGGCTTCAACGAGTGGGACATCTCGCGCGACGCCCCC
TATTTCGGCTTCGAGATCCCCGACGCGCCGGGCAAGTATTTCTATGTCTGGCTGGACGCGCCGATCGGCTACCTGGCCAG
CTTCCAGAACCTCTGCGAGCGTGAGGGCATCGACTTCGACAGCTACTGGCGCCGCGACTCGGACGCGGAGGTCTATCACT
TCATCGGCAAGGACATCGTCTACTTCCACGCCCTGTTCTGGCCGGCCATGCTCGAGGGCGCTGGGCTGCGCACGCCGACC
GGCGTCAACTGCCACGGCTTCGTCACCGTCAACGGCGCCAAGATGTCCAAGTCCCGCGGGACCTTCATCAAGGCGCAGAC
CTATGCCGAATATCTCAACCCCGAGTACCTGCGCTACTACTTCGCCGCCAAGCTCACCGCCGGCGTCGACGATCTGGACC
TCAACCTCGAGGACTTCGCCTCGCGCGTGAACGCGGATCTGGTCGGCAAGGTCGTCAACATCGCCAGTCGCTGTGCCGGC
TTCGTCAAGAAGCTGGGTGCCGGCCGACTCGCCGCGCACTGTGCCGAGCCCGAGCTGGTGACCCGCTTCGTGGAGGCCGG
CGAGGCCATCGCCGCCGATTACGAGGCGCGCGAATTCGCCCGCGCCATGCGCAAGGTCATGGACCTGGCCGACGAAGCCA
ATGCCTACATCGCCGACAAGGCGCCCTGGGTGCTGGCCAAGGAAGAAGGCCGCGAGCAGGAGGTGCTCGACATCTGCTCG
GTGGGCATCAACCTGTTCCGCATCCTGATGGTCTACCTGCAGCCCGTGGTGCCGGCGATGGCCGAGCAGGCACGCGACTT
CCTGCAGATCGAGTCGCTCGACTGGGAAAGCCGCCGCACCTTGCTCGAGGACCACGCGATCGCCAAGTTCAAGCCGCTGA
TGACACGTGTCGATACCGACAAGATCGCGTCGATGACCGAGGCGTCCAAGGAAGTCCTCGCCGAAGAGCAGAAGCTCAAG
GAACAGGCCAAGGGCCCCCTCGCCGACGATCCGATCGCCGACGAGATCGGCTTCGACGATTTTGCCAAGCTCGACCTGCG
CATCGTGCGCATCGCCAAGGCCGAATACGTCGAGGGCGCCAAGAAGCTCCTCAAGCTGACGCTCGACCTGGGCGGCGAAA
CGCGGACGGTCTTCTCGGGCATTCGCGCCGTCTATGCGCCCGAGGCGCTGGAAGGCCGCCTGACCGTCATGGTCGCCAAT
CTGGCACCGCGCAAGATGCGTTTCGGCGTTTCGGAAGGCATGGTGCTGGCCGCCGGCGACAAGGAGGGCGGCATCTATCT
GCTGTCACCGGACAACGGGGCGGAGCCCGGCCAGCGGGTGTCCTGA

Upstream 100 bases:

>100_bases
CCACGGATGCAGCCAAGCGCCAAGCGGGCTACAATCGCCTTTTCGTCGATGTCGTTCGAGACACCTTTTCTTGTTCCCGA
TTTCAGTCACTAGAGCCGCC

Downstream 100 bases:

>100_bases
GGAGGCATGGTGTCCGCTAACGCCGCGCTCATCGAGACGCTGGATGCCGCGCTGCCGCAGACCCAATGCGGCAAGTGCGG
GCATCCCGGCTGTCGTCCCT

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 681; Mature: 680

Protein sequence:

>681_residues
MPTTANAKRKILVTSALPYANGSIHLGHLLEYIQTDIWVRFQKSRGHECHYVCADDAHGTAIMLRAEQEGITPEQLISKV
SAEHQADFARFGVAFDNYYSTHSEENRQHSERIYTALRDAGHISTRDIEQMYDPVKGLFLADRFIKGTCPKCGAGDQYGD
NCEACGATYTPAELIDPVSAISGATPEVRSSTHFFFKLPDFADFMQGWIDGGHVQPQIRNKLLEWFESGFNEWDISRDAP
YFGFEIPDAPGKYFYVWLDAPIGYLASFQNLCEREGIDFDSYWRRDSDAEVYHFIGKDIVYFHALFWPAMLEGAGLRTPT
GVNCHGFVTVNGAKMSKSRGTFIKAQTYAEYLNPEYLRYYFAAKLTAGVDDLDLNLEDFASRVNADLVGKVVNIASRCAG
FVKKLGAGRLAAHCAEPELVTRFVEAGEAIAADYEAREFARAMRKVMDLADEANAYIADKAPWVLAKEEGREQEVLDICS
VGINLFRILMVYLQPVVPAMAEQARDFLQIESLDWESRRTLLEDHAIAKFKPLMTRVDTDKIASMTEASKEVLAEEQKLK
EQAKGPLADDPIADEIGFDDFAKLDLRIVRIAKAEYVEGAKKLLKLTLDLGGETRTVFSGIRAVYAPEALEGRLTVMVAN
LAPRKMRFGVSEGMVLAAGDKEGGIYLLSPDNGAEPGQRVS

Sequences:

>Translated_681_residues
MPTTANAKRKILVTSALPYANGSIHLGHLLEYIQTDIWVRFQKSRGHECHYVCADDAHGTAIMLRAEQEGITPEQLISKV
SAEHQADFARFGVAFDNYYSTHSEENRQHSERIYTALRDAGHISTRDIEQMYDPVKGLFLADRFIKGTCPKCGAGDQYGD
NCEACGATYTPAELIDPVSAISGATPEVRSSTHFFFKLPDFADFMQGWIDGGHVQPQIRNKLLEWFESGFNEWDISRDAP
YFGFEIPDAPGKYFYVWLDAPIGYLASFQNLCEREGIDFDSYWRRDSDAEVYHFIGKDIVYFHALFWPAMLEGAGLRTPT
GVNCHGFVTVNGAKMSKSRGTFIKAQTYAEYLNPEYLRYYFAAKLTAGVDDLDLNLEDFASRVNADLVGKVVNIASRCAG
FVKKLGAGRLAAHCAEPELVTRFVEAGEAIAADYEAREFARAMRKVMDLADEANAYIADKAPWVLAKEEGREQEVLDICS
VGINLFRILMVYLQPVVPAMAEQARDFLQIESLDWESRRTLLEDHAIAKFKPLMTRVDTDKIASMTEASKEVLAEEQKLK
EQAKGPLADDPIADEIGFDDFAKLDLRIVRIAKAEYVEGAKKLLKLTLDLGGETRTVFSGIRAVYAPEALEGRLTVMVAN
LAPRKMRFGVSEGMVLAAGDKEGGIYLLSPDNGAEPGQRVS
>Mature_680_residues
PTTANAKRKILVTSALPYANGSIHLGHLLEYIQTDIWVRFQKSRGHECHYVCADDAHGTAIMLRAEQEGITPEQLISKVS
AEHQADFARFGVAFDNYYSTHSEENRQHSERIYTALRDAGHISTRDIEQMYDPVKGLFLADRFIKGTCPKCGAGDQYGDN
CEACGATYTPAELIDPVSAISGATPEVRSSTHFFFKLPDFADFMQGWIDGGHVQPQIRNKLLEWFESGFNEWDISRDAPY
FGFEIPDAPGKYFYVWLDAPIGYLASFQNLCEREGIDFDSYWRRDSDAEVYHFIGKDIVYFHALFWPAMLEGAGLRTPTG
VNCHGFVTVNGAKMSKSRGTFIKAQTYAEYLNPEYLRYYFAAKLTAGVDDLDLNLEDFASRVNADLVGKVVNIASRCAGF
VKKLGAGRLAAHCAEPELVTRFVEAGEAIAADYEAREFARAMRKVMDLADEANAYIADKAPWVLAKEEGREQEVLDICSV
GINLFRILMVYLQPVVPAMAEQARDFLQIESLDWESRRTLLEDHAIAKFKPLMTRVDTDKIASMTEASKEVLAEEQKLKE
QAKGPLADDPIADEIGFDDFAKLDLRIVRIAKAEYVEGAKKLLKLTLDLGGETRTVFSGIRAVYAPEALEGRLTVMVANL
APRKMRFGVSEGMVLAAGDKEGGIYLLSPDNGAEPGQRVS

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=563, Percent_Identity=26.4653641207815, Blast_Score=226, Evalue=6e-59,
Organism=Homo sapiens, GI24308436, Length=548, Percent_Identity=25.3649635036496, Blast_Score=162, Evalue=7e-40,
Organism=Escherichia coli, GI1788432, Length=675, Percent_Identity=61.4814814814815, Blast_Score=900, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=626, Percent_Identity=27.6357827476038, Blast_Score=239, Evalue=3e-63,
Organism=Caenorhabditis elegans, GI212645096, Length=519, Percent_Identity=24.6628131021195, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=343, Percent_Identity=27.1137026239067, Blast_Score=108, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6321704, Length=559, Percent_Identity=28.4436493738819, Blast_Score=241, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6321610, Length=547, Percent_Identity=22.4862888482633, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24655311, Length=565, Percent_Identity=27.787610619469, Blast_Score=231, Evalue=2e-60,
Organism=Drosophila melanogaster, GI21358483, Length=523, Percent_Identity=25.0478011472275, Blast_Score=152, Evalue=1e-36,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_CHRSD (Q1QVN7)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_574170.1
- ProteinModelPortal:   Q1QVN7
- STRING:   Q1QVN7
- GeneID:   4029265
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_2120
- NMPDR:   fig|290398.4.peg.2254
- eggNOG:   COG0143
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- BioCyc:   CSAL290398:CSAL_2120-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398
- TIGRFAMs:   TIGR00399

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 75914; Mature: 75783

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 337-337

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTTANAKRKILVTSALPYANGSIHLGHLLEYIQTDIWVRFQKSRGHECHYVCADDAHGT
CCCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCC
AIMLRAEQEGITPEQLISKVSAEHQADFARFGVAFDNYYSTHSEENRQHSERIYTALRDA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHC
GHISTRDIEQMYDPVKGLFLADRFIKGTCPKCGAGDQYGDNCEACGATYTPAELIDPVSA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCHHHHCHHHH
ISGATPEVRSSTHFFFKLPDFADFMQGWIDGGHVQPQIRNKLLEWFESGFNEWDISRDAP
HCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
YFGFEIPDAPGKYFYVWLDAPIGYLASFQNLCEREGIDFDSYWRRDSDAEVYHFIGKDIV
CCCEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHH
YFHALFWPAMLEGAGLRTPTGVNCHGFVTVNGAKMSKSRGTFIKAQTYAEYLNPEYLRYY
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEECCCCCCEEEHHHHHHHCCHHHHHHH
FAAKLTAGVDDLDLNLEDFASRVNADLVGKVVNIASRCAGFVKKLGAGRLAAHCAEPELV
HHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHH
TRFVEAGEAIAADYEAREFARAMRKVMDLADEANAYIADKAPWVLAKEEGREQEVLDICS
HHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCHHHHHHHHH
VGINLFRILMVYLQPVVPAMAEQARDFLQIESLDWESRRTLLEDHAIAKFKPLMTRVDTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
KIASMTEASKEVLAEEQKLKEQAKGPLADDPIADEIGFDDFAKLDLRIVRIAKAEYVEGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KKLLKLTLDLGGETRTVFSGIRAVYAPEALEGRLTVMVANLAPRKMRFGVSEGMVLAAGD
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCEEEEEEECCCCHHHHCCCCCCEEEEECC
KEGGIYLLSPDNGAEPGQRVS
CCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
PTTANAKRKILVTSALPYANGSIHLGHLLEYIQTDIWVRFQKSRGHECHYVCADDAHGT
CCCCCCCEEEEEEECCCCCCCCEEHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCCCC
AIMLRAEQEGITPEQLISKVSAEHQADFARFGVAFDNYYSTHSEENRQHSERIYTALRDA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHHHHHHC
GHISTRDIEQMYDPVKGLFLADRFIKGTCPKCGAGDQYGDNCEACGATYTPAELIDPVSA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCHHHHCHHHH
ISGATPEVRSSTHFFFKLPDFADFMQGWIDGGHVQPQIRNKLLEWFESGFNEWDISRDAP
HCCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
YFGFEIPDAPGKYFYVWLDAPIGYLASFQNLCEREGIDFDSYWRRDSDAEVYHFIGKDIV
CCCEECCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHH
YFHALFWPAMLEGAGLRTPTGVNCHGFVTVNGAKMSKSRGTFIKAQTYAEYLNPEYLRYY
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCEECCCCCCEEEHHHHHHHCCHHHHHHH
FAAKLTAGVDDLDLNLEDFASRVNADLVGKVVNIASRCAGFVKKLGAGRLAAHCAEPELV
HHHHHHCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHH
TRFVEAGEAIAADYEAREFARAMRKVMDLADEANAYIADKAPWVLAKEEGREQEVLDICS
HHHHHCCCHHHCCHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEECCCCCHHHHHHHHH
VGINLFRILMVYLQPVVPAMAEQARDFLQIESLDWESRRTLLEDHAIAKFKPLMTRVDTD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHH
KIASMTEASKEVLAEEQKLKEQAKGPLADDPIADEIGFDDFAKLDLRIVRIAKAEYVEGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KKLLKLTLDLGGETRTVFSGIRAVYAPEALEGRLTVMVANLAPRKMRFGVSEGMVLAAGD
HHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCEEEEEEECCCCHHHHCCCCCCEEEEECC
KEGGIYLLSPDNGAEPGQRVS
CCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA