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The map label for this gene is fliJ [H]

Identifier: 92114082

GI number: 92114082

Start: 2216584

End: 2217081

Strand: Direct

Name: fliJ [H]

Synonym: Csal_1959

Alternate gene names: 92114082

Gene position: 2216584-2217081 (Clockwise)

Preceding gene: 92114081

Following gene: 92114083

Centisome position: 59.96

GC content: 62.85

Gene sequence:

>498_bases
ATGAACGCACGCTCCCCGCTGACCACACTCAAGGATCTTGCGCAGGATACCTGCGACCAGGCCGCGCAATCGCTGGGGCA
TTCACGGCGCAAGCTGCAGGAAGCCCAGCAGCGCCTGGATCAGTTGCTGACCTATCGGCACGAATATCGCCAGCGCCTGG
AAAACAGCATGCAGCAGGGCATCGAGCCTGCCAACTGGCACAACTATCAACAGTTTCTGGCCTCGCTCGACCAGGCGATC
GCCGGTCAGCGCCAGGCCGTCAACGATCAGCAGCGCCACCTCGACCGCCAACAGCAGGCATGGCAGGGTGAGCGTCGCAA
GCTCAACACCTACGACACGCTGATCACGCGCCGTGACAACGCCGAGCAACAACGCGCGGCGCGGCGCGAGCAGCAACTCT
TCGACGAAATGTCGGCGCGCATGACACGCCGCCAAGCGAACGACCGTGGCCTCGATGTCACAACGACCGATTCGTCCGCT
TCCACTACCCGCTTTTGA

Upstream 100 bases:

>100_bases
GACGAACGCGCCGATGTCGACTCGACGCTCGACGCCCTGGCCTCGCTGTTTCCGGAGACGCGCTGATGCCATTCACCCTG
CATTGCGGTAAGGAGGTCTC

Downstream 100 bases:

>100_bases
CGAGGCATGCACATGGATATTGCCCTGCTCAACACCCTTCAAGGCGGTAACCAAGCCTCCGCCACCACGCCCGGCAGCGC
GGGAAGTCAGCGCGGCGGCA

Product: flagellar export FliJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MNARSPLTTLKDLAQDTCDQAAQSLGHSRRKLQEAQQRLDQLLTYRHEYRQRLENSMQQGIEPANWHNYQQFLASLDQAI
AGQRQAVNDQQRHLDRQQQAWQGERRKLNTYDTLITRRDNAEQQRAARREQQLFDEMSARMTRRQANDRGLDVTTTDSSA
STTRF

Sequences:

>Translated_165_residues
MNARSPLTTLKDLAQDTCDQAAQSLGHSRRKLQEAQQRLDQLLTYRHEYRQRLENSMQQGIEPANWHNYQQFLASLDQAI
AGQRQAVNDQQRHLDRQQQAWQGERRKLNTYDTLITRRDNAEQQRAARREQQLFDEMSARMTRRQANDRGLDVTTTDSSA
STTRF
>Mature_165_residues
MNARSPLTTLKDLAQDTCDQAAQSLGHSRRKLQEAQQRLDQLLTYRHEYRQRLENSMQQGIEPANWHNYQQFLASLDQAI
AGQRQAVNDQQRHLDRQQQAWQGERRKLNTYDTLITRRDNAEQQRAARREQQLFDEMSARMTRRQANDRGLDVTTTDSSA
STTRF

Specific function: The fliJ protein is a flagellar protein that affects chemotactic events. Mutations in fliJ results in failure to respond to chemotactic stimuli [H]

COG id: COG2882

COG function: function code NUO; Flagellar biosynthesis chaperone

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliJ family [H]

Homologues:

Organism=Escherichia coli, GI1788252, Length=145, Percent_Identity=35.1724137931034, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018006
- InterPro:   IPR012823 [H]

Pfam domain/function: PF02050 FliJ [H]

EC number: NA

Molecular weight: Translated: 19390; Mature: 19390

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARSPLTTLKDLAQDTCDQAAQSLGHSRRKLQEAQQRLDQLLTYRHEYRQRLENSMQQG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IEPANWHNYQQFLASLDQAIAGQRQAVNDQQRHLDRQQQAWQGERRKLNTYDTLITRRDN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEQQRAARREQQLFDEMSARMTRRQANDRGLDVTTTDSSASTTRF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MNARSPLTTLKDLAQDTCDQAAQSLGHSRRKLQEAQQRLDQLLTYRHEYRQRLENSMQQG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
IEPANWHNYQQFLASLDQAIAGQRQAVNDQQRHLDRQQQAWQGERRKLNTYDTLITRRDN
CCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AEQQRAARREQQLFDEMSARMTRRQANDRGLDVTTTDSSASTTRF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8631687 [H]