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The map label for this gene is narI [H]

Identifier: 92113459

GI number: 92113459

Start: 1526103

End: 1526780

Strand: Direct

Name: narI [H]

Synonym: Csal_1333

Alternate gene names: 92113459

Gene position: 1526103-1526780 (Clockwise)

Preceding gene: 92113458

Following gene: 92113460

Centisome position: 41.28

GC content: 60.32

Gene sequence:

>678_bases
ATGAATTATCTCAACGAACTGTTGTTTGGCCTCTATCCCTATGTGGCGGGCACCGTCTTCCTGATCGGCAGTCTGCTGCG
CTTCGATCACGGCCAATACACCTGGAAGGCCGGTTCCAGCCAACTGCTGGACCGAAAGCGAATGCGTCTGGGCAGCAACC
TGTTCCATATCGGCATACTCGTCATCCTTGTCGGTCACCTGGTCGGCCTGCTGACTCCGCATTGGGTTTACGCGCCCTTC
ATCTCGGCCGGACACAAACAGGTTCTCGCCATTCTGGTCGGCGGCATCGCCGGGGTGATGTGCCTGGCGGGAGGCATGAT
CCTGCTTCACCGTCGTCTGACCAATCCGCGTGTGCGCGCTTCGTCGAGCCGTGCCGACATCTTCATCCTGGCGCTGTTGA
TGGTGCAGGTTAGCCTGGGGCTCTTGACCATTCCCTTCGCCATGCACCATCTCGACGGCGGCGTGATGCTGCTGCTGGCG
GATTGGGCGCAGTCGATCGTGTTCTTCAAGGGAGGCGCCGCCGAGCATCTCGCCGAGGTCGGGGGGATCTACAAACTGCA
CATCCTGGTGGGACTGACCATCATTCTGGTCTTCCCCTTCACTCGCCTGGTGCATGTCTGGAGTGCACCACTGGGGTATC
TCGGACGGCGTTATCAGGTCGTCCGCAAGCGCGGCTGA

Upstream 100 bases:

>100_bases
CCGCAGAGGGACGCAGACTCAACGAGCGCAAGAAGATGACGCAAAGCGAAGCGGTGAGGGTCTTGGATCCTGTCGCCCGT
CAGGCGCGAGGAGATTCGCC

Downstream 100 bases:

>100_bases
AGCGGGCCTTGCCAACGGAAACGCTCCCGATCGTCGGAACGGTTGAGAAGAACTTCAAGGATTAAGCCATGCAGAAGATT
GCTATCGAGCAGATTCCGGG

Product: respiratory nitrate reductase gamma subunit

Products: NA

Alternate protein names: Cytochrome B-NR; Nitrate reductase A subunit gamma; Quinol-nitrate oxidoreductase subunit gamma [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MNYLNELLFGLYPYVAGTVFLIGSLLRFDHGQYTWKAGSSQLLDRKRMRLGSNLFHIGILVILVGHLVGLLTPHWVYAPF
ISAGHKQVLAILVGGIAGVMCLAGGMILLHRRLTNPRVRASSSRADIFILALLMVQVSLGLLTIPFAMHHLDGGVMLLLA
DWAQSIVFFKGGAAEHLAEVGGIYKLHILVGLTIILVFPFTRLVHVWSAPLGYLGRRYQVVRKRG

Sequences:

>Translated_225_residues
MNYLNELLFGLYPYVAGTVFLIGSLLRFDHGQYTWKAGSSQLLDRKRMRLGSNLFHIGILVILVGHLVGLLTPHWVYAPF
ISAGHKQVLAILVGGIAGVMCLAGGMILLHRRLTNPRVRASSSRADIFILALLMVQVSLGLLTIPFAMHHLDGGVMLLLA
DWAQSIVFFKGGAAEHLAEVGGIYKLHILVGLTIILVFPFTRLVHVWSAPLGYLGRRYQVVRKRG
>Mature_225_residues
MNYLNELLFGLYPYVAGTVFLIGSLLRFDHGQYTWKAGSSQLLDRKRMRLGSNLFHIGILVILVGHLVGLLTPHWVYAPF
ISAGHKQVLAILVGGIAGVMCLAGGMILLHRRLTNPRVRASSSRADIFILALLMVQVSLGLLTIPFAMHHLDGGVMLLLA
DWAQSIVFFKGGAAEHLAEVGGIYKLHILVGLTIILVFPFTRLVHVWSAPLGYLGRRYQVVRKRG

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit [

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787480, Length=224, Percent_Identity=58.0357142857143, Blast_Score=281, Evalue=2e-77,
Organism=Escherichia coli, GI1787738, Length=224, Percent_Identity=56.25, Blast_Score=269, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 24817; Mature: 24817

Theoretical pI: Translated: 10.81; Mature: 10.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLNELLFGLYPYVAGTVFLIGSLLRFDHGQYTWKAGSSQLLDRKRMRLGSNLFHIGIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHH
VILVGHLVGLLTPHWVYAPFISAGHKQVLAILVGGIAGVMCLAGGMILLHRRLTNPRVRA
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SSSRADIFILALLMVQVSLGLLTIPFAMHHLDGGVMLLLADWAQSIVFFKGGAAEHLAEV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
GGIYKLHILVGLTIILVFPFTRLVHVWSAPLGYLGRRYQVVRKRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNYLNELLFGLYPYVAGTVFLIGSLLRFDHGQYTWKAGSSQLLDRKRMRLGSNLFHIGIL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHCCCHHHHHHH
VILVGHLVGLLTPHWVYAPFISAGHKQVLAILVGGIAGVMCLAGGMILLHRRLTNPRVRA
HHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
SSSRADIFILALLMVQVSLGLLTIPFAMHHLDGGVMLLLADWAQSIVFFKGGAAEHLAEV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
GGIYKLHILVGLTIILVFPFTRLVHVWSAPLGYLGRRYQVVRKRG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2832376; 8905232; 9278503; 3053688; 12910261 [H]