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The map label for this gene is narY [H]

Identifier: 92113457

GI number: 92113457

Start: 1523775

End: 1525334

Strand: Direct

Name: narY [H]

Synonym: Csal_1331

Alternate gene names: 92113457

Gene position: 1523775-1525334 (Clockwise)

Preceding gene: 92113456

Following gene: 92113458

Centisome position: 41.22

GC content: 62.95

Gene sequence:

>1560_bases
ATGAAGATTCGTTCCCAGGTCGGCATGGTCCTCAACCTCGACAAGTGCATCGGCTGCCATACCTGCTCGGTCACCTGCAA
GAACGTCTGGACCAGTCGCGAGGGCGTCGAGTACGCCTGGTTCAACAACGTCGAGACCAAGCCCGGCATCGGTTATCCCA
AGGAGTGGGAGAACCAGCACAGATGGAAGGGCGGCTGGCTGCGCCGCAAGGATGGCCATATCGAGCCGCGTATCGGCGGC
AAGTGGCGGGTGCTGGCGAATATCTTCGCCAACCCCGACTTGCCCGAGATCGACGACTACTACGAGCCATTCACCTTCGA
TTATGAACATTTGCATACTGCCAGGCAGGGTGAACATCAGCCCACGGCCCGGCCGCGTTCGCTGATCTCCGGCCAGCGCA
TGAACAAGATCGAGTGGGGACCCAACTGGGAAGAGATTCTCGGTACCGAGTTCGCCAAGCGCCGTCGTGACGCCAACTTC
GAGCAGGTGCAGGCCGATATCTACGGCCAGTTCGAGAACACCTTCATGATGTACCTGCCGCGGCTCTGCGAGCATTGCCT
GAACCCGACCTGCGTGGCGTCCTGTCCGAGCGGGGCGATCTACAAGCGCGAGGAAGACGGCATCGTGCTGATCGACCAGG
ACAAGTGCCGCGGCTGGCGGATGTGCATCTCCGGCTGCCCGTACAAGAAGATCTATTACAACTGGAAGAGCGGCAAGTCC
GAGAAGTGCATCTTCTGCTACCCGCGCATCGAGGCCGGTCAGCCGACCATCTGCTCCGAGACCTGCGTGGGCCGTATCCG
CTACCTGGGGGTGTTGCTCTATGACGCCGACCGCATCGAGGAAGTGGCAAGCTCGCCCGACGAGCAGGATCTCTACCATC
GCCAGCGCGAGATCTTTCTCGATCCCCACGATCCCGAGGTGATCGCCCAGGCCCGCAAGGACGGCGTCGCCGACAGCGTG
ATCACCGCCGCCCAGCAGAGCCCGGTCTACAAGCTGGCCATGGAATGGGGCCTGGCGCTGCCGCTGCACCCGGAGTATCG
CACCCTGCCGATGGTCTGGTACGTGCCGCCGCTGTCGCCGATCCAGTCTGCCGCCGAGGCCGGCCAGGTCGAGTACGACG
GCGTGCTGCCGAAGGTCGAGTCGCTGCGCATCCCGGTGAAGTATCTGGCCAACCTGCTGACCGCCGGCGAGGAGGCCCCT
GTGGTGCTGGCGCTCAAGCGTCTGATGGCGATGCGGATTTACATGCGCGGCAAGCATGTGGAAGGCCAGGTGGATACCTC
CGTGCTGGACGAGGTCGAGCTGAGCGAAGCTCAGGTAGAAGACATGTATCGCTATCTGGCCATCGCCAACTATGAGGATC
GCTTCGTGATCCCCACCAGCCACCGCGAGATGGCCGTCGATGCCTTCCCCGAGCGCGGCGGCTGCGGCTTCAGCTTCGGC
AACGGCTGCCACGGTGGTGACAACGACGCCAGCCTGTTCGGAGGCCGCAAGCAGACCACCACCCTGGTTCGACCGGTGGA
AACCTATGATCCACAAGCCGAGGAGGCCCGTCATGGCTGA

Upstream 100 bases:

>100_bases
CCGTCGGCTCCAACCGCGACGAGTTCGTCATCGTGCGCAAGATGAAGAAGATCGACTGGCTGGATGGCGAAGGCAACGAC
TACGAGCAGGAGGCCGTGAA

Downstream 100 bases:

>100_bases
TACCGCTCCCGATATGGCGTCGCTCGGTGGCATGCGTAGCCTGCGCGTGCTGGCACGGCTGCTGGATTATCCCTGCGAAG
CGCTCCAGGCCGCGGCCGAC

Product: respiratory nitrate reductase beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQHRWKGGWLRRKDGHIEPRIGG
KWRVLANIFANPDLPEIDDYYEPFTFDYEHLHTARQGEHQPTARPRSLISGQRMNKIEWGPNWEEILGTEFAKRRRDANF
EQVQADIYGQFENTFMMYLPRLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
EKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYDADRIEEVASSPDEQDLYHRQREIFLDPHDPEVIAQARKDGVADSV
ITAAQQSPVYKLAMEWGLALPLHPEYRTLPMVWYVPPLSPIQSAAEAGQVEYDGVLPKVESLRIPVKYLANLLTAGEEAP
VVLALKRLMAMRIYMRGKHVEGQVDTSVLDEVELSEAQVEDMYRYLAIANYEDRFVIPTSHREMAVDAFPERGGCGFSFG
NGCHGGDNDASLFGGRKQTTTLVRPVETYDPQAEEARHG

Sequences:

>Translated_519_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQHRWKGGWLRRKDGHIEPRIGG
KWRVLANIFANPDLPEIDDYYEPFTFDYEHLHTARQGEHQPTARPRSLISGQRMNKIEWGPNWEEILGTEFAKRRRDANF
EQVQADIYGQFENTFMMYLPRLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
EKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYDADRIEEVASSPDEQDLYHRQREIFLDPHDPEVIAQARKDGVADSV
ITAAQQSPVYKLAMEWGLALPLHPEYRTLPMVWYVPPLSPIQSAAEAGQVEYDGVLPKVESLRIPVKYLANLLTAGEEAP
VVLALKRLMAMRIYMRGKHVEGQVDTSVLDEVELSEAQVEDMYRYLAIANYEDRFVIPTSHREMAVDAFPERGGCGFSFG
NGCHGGDNDASLFGGRKQTTTLVRPVETYDPQAEEARHG
>Mature_519_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQHRWKGGWLRRKDGHIEPRIGG
KWRVLANIFANPDLPEIDDYYEPFTFDYEHLHTARQGEHQPTARPRSLISGQRMNKIEWGPNWEEILGTEFAKRRRDANF
EQVQADIYGQFENTFMMYLPRLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
EKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYDADRIEEVASSPDEQDLYHRQREIFLDPHDPEVIAQARKDGVADSV
ITAAQQSPVYKLAMEWGLALPLHPEYRTLPMVWYVPPLSPIQSAAEAGQVEYDGVLPKVESLRIPVKYLANLLTAGEEAP
VVLALKRLMAMRIYMRGKHVEGQVDTSVLDEVELSEAQVEDMYRYLAIANYEDRFVIPTSHREMAVDAFPERGGCGFSFG
NGCHGGDNDASLFGGRKQTTTLVRPVETYDPQAEEARHG

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=498, Percent_Identity=76.7068273092369, Blast_Score=822, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=498, Percent_Identity=74.6987951807229, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=44.4444444444444, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787122, Length=101, Percent_Identity=43.5643564356436, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=29.7297297297297, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1789370, Length=177, Percent_Identity=31.0734463276836, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=37.2093023255814, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 59238; Mature: 59238

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQH
CCCHHHCCCEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHCCCC
RWKGGWLRRKDGHIEPRIGGKWRVLANIFANPDLPEIDDYYEPFTFDYEHLHTARQGEHQ
CCCCCCEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCHHCCCCCCCCHHHHHHHHCCCCC
PTARPRSLISGQRMNKIEWGPNWEEILGTEFAKRRRDANFEQVQADIYGQFENTFMMYLP
CCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
HHHHHHCCCEEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECCCCC
EKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYDADRIEEVASSPDEQDLYHRQREIFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHCCCCHHHHHHHHHHEEC
DPHDPEVIAQARKDGVADSVITAAQQSPVYKLAMEWGLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHH
IQSAAEAGQVEYDGVLPKVESLRIPVKYLANLLTAGEEAPVVLALKRLMAMRIYMRGKHV
HHHHHHCCCEEECCCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
EGQVDTSVLDEVELSEAQVEDMYRYLAIANYEDRFVIPTSHREMAVDAFPERGGCGFSFG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHCCCCCCCCCCCCCC
NGCHGGDNDASLFGGRKQTTTLVRPVETYDPQAEEARHG
CCCCCCCCCHHHCCCCCCCCEEECCHHHCCCCHHHCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNVETKPGIGYPKEWENQH
CCCHHHCCCEEEHHHHCCCCEEEEEHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHCCCC
RWKGGWLRRKDGHIEPRIGGKWRVLANIFANPDLPEIDDYYEPFTFDYEHLHTARQGEHQ
CCCCCCEECCCCCCCCCCCCCEEEEEHHHCCCCCCCCHHCCCCCCCCHHHHHHHHCCCCC
PTARPRSLISGQRMNKIEWGPNWEEILGTEFAKRRRDANFEQVQADIYGQFENTFMMYLP
CCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVASCPSGAIYKREEDGIVLIDQDKCRGWRMCISGCPYKKIYYNWKSGKS
HHHHHHCCCEEEECCCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCCCEEEEECCCCC
EKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYDADRIEEVASSPDEQDLYHRQREIFL
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHCCCCHHHHHHHHHHEEC
DPHDPEVIAQARKDGVADSVITAAQQSPVYKLAMEWGLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHH
IQSAAEAGQVEYDGVLPKVESLRIPVKYLANLLTAGEEAPVVLALKRLMAMRIYMRGKHV
HHHHHHCCCEEECCCCCCHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
EGQVDTSVLDEVELSEAQVEDMYRYLAIANYEDRFVIPTSHREMAVDAFPERGGCGFSFG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHCCCCCCCCCCCCCC
NGCHGGDNDASLFGGRKQTTTLVRPVETYDPQAEEARHG
CCCCCCCCCHHHCCCCCCCCEEECCHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]