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The map label for this gene is lpdG [H]

Identifier: 92113345

GI number: 92113345

Start: 1396665

End: 1398104

Strand: Direct

Name: lpdG [H]

Synonym: Csal_1219

Alternate gene names: 92113345

Gene position: 1396665-1398104 (Clockwise)

Preceding gene: 92113344

Following gene: 92113346

Centisome position: 37.78

GC content: 62.71

Gene sequence:

>1440_bases
ATGGCCGATAAATTCGATGTGATCGTTATTGGCGCCGGGCCTGGCGGGTACGTGGCAGCCATTCGTGCCGCGCAGCTGGG
GTTGAAGACCGCCTGTGTCGAGAAGTGGGTCAACAAGGAAGGCAAGACCGTGCACGGCGGTACTTGTCTGAATGTGGGCT
GCATTCCCTCGAAGGCGTTGCTGGAAACGTCCCACAAGTTCGCCGAGGCTCGTGATCACTTCGCCGAGATTGGCATCGAC
GCCGGCGAGCCGACCCCGAACATTGCCAAGATGCTCGAGTTCAAGGACGGTGTGATCGCCAAGAACGTGGGCGGCATCAG
CGCACTGTTCAAGGCCAATGGCGTCACTGCGCTGGAAGGCACCGGCAAGGTCGTGTCCAGCAAGCAGGTCGAGGTGACCG
GCCACGACGGCGAGACCGCTTCCTACGAGGCCGAGAACATCGTCATTGCCTCCGGCTCGGTGCCGGTGGAAATTCCGCCG
ACGCCGCTGACCGAAGGATTGATCGTCGATTCCAGCGGTGCCCTCAAGTTCGATGAGGTGCCCAAGCGCCTCGGCGTCAT
CGGCGCCGGCGTCATCGGCCTCGAGCTGGGCAGCGTATGGAGCCGCCTGGGCAGCGATGTCACGGTCCTCGAAGCGATGG
ATTCATTCCTGCCGATGGTCGATAAGGACGTCGCCAAGGAAGCGCAGAAACTGTTCAAGAAGCAGGGCCTGGACATCAAG
CTGGGAGCACGGGTCACCGGTTCCGAGGTCAAGGACAAGGAAGTCGTGGTCAAGTACACCGACGCCAACGGCGAACAGGA
ACAGACCTTCGACAAGCTGATCGTCTGCGTGGGGCGTCGTCCCTACACGGAAGGCGTGCTCAGCGACGACGTGGGCGTCA
AGCTCGACGAGCGCGGGTCCGTGTTCGTCGACGACGAATGCCGCACGAGCGTCCCGGGCGTTTACGCCATCGGCGATGTG
GTGCGCGGGCCGATGCTGGCGCACAAGGCCTCCGAGGAAGGCATGATGGTTGCCGATATCATCGCCGGTCATAAGGCCGA
GATGAACTACGACGCCATCCCCAGCGTGATCTATACCGCACCCGAAGTCGCCTGGGTCGGCATGAACGAGCAGGACGCCA
AAGAAGCCGGAATCGAGGTCAAGACCGGCTCCTTCCCGTTCTCCGCCAACGGTCGTGCGCTGGCCAACAATGCGCCGGAG
GGCATGGCCAAGATCATCGCCGATGCCGAGACCGACCGTGTCCTGGGGCTGCATATCATCAGTCAGCATGCCGGGGAGCT
CATCGCGCAAGGCGTCATCGCCATGGAGTTCGGCTCCAGCGCCGAGGACCTGGCCCTGACATGCTACGCGCACCCGAGCA
CCTCGGAAGCCGTGCACGAAGCGGCCCTGGCGGTGGATGGTCACGCCATTCACATGGCCAACCGCAAGAAGCGCAAGTAA

Upstream 100 bases:

>100_bases
GGAATTGCTGGAAGATCCCGCGCGCTTCTTGCTCGACGTGTGATGGCCGTCGCCGAATGATGCGTGCGACTTTTAAACTG
AATGCGAACAGGATGATTTC

Downstream 100 bases:

>100_bases
GCGTTTCGATCTGCGTTTCAGTGTAGAGACGCGTCGCTCCCGCCTCGGCGGGAGCGAACAGGGGTGACGGCGACCTCGTG
GGTGGCCGCCACCATTCGAG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MADKFDVIVIGAGPGGYVAAIRAAQLGLKTACVEKWVNKEGKTVHGGTCLNVGCIPSKALLETSHKFAEARDHFAEIGID
AGEPTPNIAKMLEFKDGVIAKNVGGISALFKANGVTALEGTGKVVSSKQVEVTGHDGETASYEAENIVIASGSVPVEIPP
TPLTEGLIVDSSGALKFDEVPKRLGVIGAGVIGLELGSVWSRLGSDVTVLEAMDSFLPMVDKDVAKEAQKLFKKQGLDIK
LGARVTGSEVKDKEVVVKYTDANGEQEQTFDKLIVCVGRRPYTEGVLSDDVGVKLDERGSVFVDDECRTSVPGVYAIGDV
VRGPMLAHKASEEGMMVADIIAGHKAEMNYDAIPSVIYTAPEVAWVGMNEQDAKEAGIEVKTGSFPFSANGRALANNAPE
GMAKIIADAETDRVLGLHIISQHAGELIAQGVIAMEFGSSAEDLALTCYAHPSTSEAVHEAALAVDGHAIHMANRKKRK

Sequences:

>Translated_479_residues
MADKFDVIVIGAGPGGYVAAIRAAQLGLKTACVEKWVNKEGKTVHGGTCLNVGCIPSKALLETSHKFAEARDHFAEIGID
AGEPTPNIAKMLEFKDGVIAKNVGGISALFKANGVTALEGTGKVVSSKQVEVTGHDGETASYEAENIVIASGSVPVEIPP
TPLTEGLIVDSSGALKFDEVPKRLGVIGAGVIGLELGSVWSRLGSDVTVLEAMDSFLPMVDKDVAKEAQKLFKKQGLDIK
LGARVTGSEVKDKEVVVKYTDANGEQEQTFDKLIVCVGRRPYTEGVLSDDVGVKLDERGSVFVDDECRTSVPGVYAIGDV
VRGPMLAHKASEEGMMVADIIAGHKAEMNYDAIPSVIYTAPEVAWVGMNEQDAKEAGIEVKTGSFPFSANGRALANNAPE
GMAKIIADAETDRVLGLHIISQHAGELIAQGVIAMEFGSSAEDLALTCYAHPSTSEAVHEAALAVDGHAIHMANRKKRK
>Mature_478_residues
ADKFDVIVIGAGPGGYVAAIRAAQLGLKTACVEKWVNKEGKTVHGGTCLNVGCIPSKALLETSHKFAEARDHFAEIGIDA
GEPTPNIAKMLEFKDGVIAKNVGGISALFKANGVTALEGTGKVVSSKQVEVTGHDGETASYEAENIVIASGSVPVEIPPT
PLTEGLIVDSSGALKFDEVPKRLGVIGAGVIGLELGSVWSRLGSDVTVLEAMDSFLPMVDKDVAKEAQKLFKKQGLDIKL
GARVTGSEVKDKEVVVKYTDANGEQEQTFDKLIVCVGRRPYTEGVLSDDVGVKLDERGSVFVDDECRTSVPGVYAIGDVV
RGPMLAHKASEEGMMVADIIAGHKAEMNYDAIPSVIYTAPEVAWVGMNEQDAKEAGIEVKTGSFPFSANGRALANNAPEG
MAKIIADAETDRVLGLHIISQHAGELIAQGVIAMEFGSSAEDLALTCYAHPSTSEAVHEAALAVDGHAIHMANRKKRK

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=465, Percent_Identity=48.1720430107527, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI50301238, Length=484, Percent_Identity=27.4793388429752, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI33519430, Length=453, Percent_Identity=26.9315673289183, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI33519428, Length=453, Percent_Identity=26.9315673289183, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI33519426, Length=453, Percent_Identity=26.9315673289183, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI148277065, Length=453, Percent_Identity=26.9315673289183, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI148277071, Length=453, Percent_Identity=26.9315673289183, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI291045266, Length=458, Percent_Identity=26.6375545851528, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI22035672, Length=464, Percent_Identity=27.801724137931, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI291045268, Length=452, Percent_Identity=25.4424778761062, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI1786307, Length=477, Percent_Identity=42.1383647798742, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI87081717, Length=466, Percent_Identity=27.8969957081545, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=28.5714285714286, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=26.4236902050114, Blast_Score=144, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=462, Percent_Identity=49.1341991341991, Blast_Score=431, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=28.4518828451883, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71983419, Length=462, Percent_Identity=28.1385281385281, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=462, Percent_Identity=28.1385281385281, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI71982272, Length=480, Percent_Identity=26.0416666666667, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=222, Percent_Identity=27.4774774774775, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=477, Percent_Identity=46.9601677148847, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6325240, Length=476, Percent_Identity=30.8823529411765, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6325166, Length=469, Percent_Identity=28.1449893390192, Blast_Score=165, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21358499, Length=463, Percent_Identity=49.244060475162, Blast_Score=429, Evalue=1e-120,
Organism=Drosophila melanogaster, GI17737741, Length=471, Percent_Identity=27.1762208067941, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640549, Length=478, Percent_Identity=28.0334728033473, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640553, Length=478, Percent_Identity=28.0334728033473, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640551, Length=478, Percent_Identity=28.0334728033473, Blast_Score=126, Evalue=4e-29,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50432; Mature: 50301

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKFDVIVIGAGPGGYVAAIRAAQLGLKTACVEKWVNKEGKTVHGGTCLNVGCIPSKAL
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCHHH
LETSHKFAEARDHFAEIGIDAGEPTPNIAKMLEFKDGVIAKNVGGISALFKANGVTALEG
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHCCCEEEECC
TGKVVSSKQVEVTGHDGETASYEAENIVIASGSVPVEIPPTPLTEGLIVDSSGALKFDEV
CCCEECCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCEEECCCCCEEHHHH
PKRLGVIGAGVIGLELGSVWSRLGSDVTVLEAMDSFLPMVDKDVAKEAQKLFKKQGLDIK
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LGARVTGSEVKDKEVVVKYTDANGEQEQTFDKLIVCVGRRPYTEGVLSDDVGVKLDERGS
ECCEECCCCCCCCEEEEEEECCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCEEECCCCC
VFVDDECRTSVPGVYAIGDVVRGPMLAHKASEEGMMVADIIAGHKAEMNYDAIPSVIYTA
EEECCCCCCCCCCEEEHHHHHCCCHHHHCCCCCCCEEEHHHCCCCCCCCCCCCCHHHCCC
PEVAWVGMNEQDAKEAGIEVKTGSFPFSANGRALANNAPEGMAKIIADAETDRVLGLHII
CCEEEECCCHHHHHHCCCEEECCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCEEEHHHH
SQHAGELIAQGVIAMEFGSSAEDLALTCYAHPSTSEAVHEAALAVDGHAIHMANRKKRK
HHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHEECCCEEEECCCCCCC
>Mature Secondary Structure 
ADKFDVIVIGAGPGGYVAAIRAAQLGLKTACVEKWVNKEGKTVHGGTCLNVGCIPSKAL
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCHHH
LETSHKFAEARDHFAEIGIDAGEPTPNIAKMLEFKDGVIAKNVGGISALFKANGVTALEG
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHCCCEEEECC
TGKVVSSKQVEVTGHDGETASYEAENIVIASGSVPVEIPPTPLTEGLIVDSSGALKFDEV
CCCEECCCEEEEECCCCCCCCCCCCEEEEECCCEEEEECCCCCCCCEEECCCCCEEHHHH
PKRLGVIGAGVIGLELGSVWSRLGSDVTVLEAMDSFLPMVDKDVAKEAQKLFKKQGLDIK
HHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
LGARVTGSEVKDKEVVVKYTDANGEQEQTFDKLIVCVGRRPYTEGVLSDDVGVKLDERGS
ECCEECCCCCCCCEEEEEEECCCCCHHHHHHHHHEEECCCCCCCCCCCCCCCEEECCCCC
VFVDDECRTSVPGVYAIGDVVRGPMLAHKASEEGMMVADIIAGHKAEMNYDAIPSVIYTA
EEECCCCCCCCCCEEEHHHHHCCCHHHHCCCCCCCEEEHHHCCCCCCCCCCCCCHHHCCC
PEVAWVGMNEQDAKEAGIEVKTGSFPFSANGRALANNAPEGMAKIIADAETDRVLGLHII
CCEEEECCCHHHHHHCCCEEECCCCCCCCCCCEECCCCCHHHHHHHHCCCCCCEEEHHHH
SQHAGELIAQGVIAMEFGSSAEDLALTCYAHPSTSEAVHEAALAVDGHAIHMANRKKRK
HHHHHHHHHCCEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHEECCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]