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The map label for this gene is sucB [H]

Identifier: 92113344

GI number: 92113344

Start: 1395024

End: 1396607

Strand: Direct

Name: sucB [H]

Synonym: Csal_1218

Alternate gene names: 92113344

Gene position: 1395024-1396607 (Clockwise)

Preceding gene: 92113343

Following gene: 92113345

Centisome position: 37.74

GC content: 63.64

Gene sequence:

>1584_bases
ATGGCTACCGAGATCAAAGCGCCAAATTTTCCAGAATCCGTCGCCGAGGGCACCATCGCCACTTGGCACAAGAAGGTCGG
TGACAGCGTCGAACGTGATGAACTGATCGTCGAGATCGAGACGGACAAGGTCGTCCTCGAGGTCGTCGCCCCGGAAGCCG
GTACCGTGACCGAGATCAAGGTCGAGGAAGGCGACACTTGCGACTCCGAGCAGGTTCTGGGGATGCTGGGCGCGGCCAGC
GAAGGCAGCAACGACAAGGCCGCCTCTCAGGAAAGCGCGGAAAAGAGCGACGACAGCGGCCAGGACACTGCACCGGCCAA
GGCCGATGCCAAGCCTGCCGCCTCCGGCAAGCAGCATGACGTGAAGGCGCCGAGCTTCCCTGAGTCCGTGCAGGAAGGCA
CCGTCGCCACCTGGCACAAGCAGGTCGGCGAAGCGGTGAAGCGTGACGAGATTCTCGCGGATATCGAGACCGACAAGGTC
GTGCTCGAAGTGGTGGCGCCGGCCGATGGTGCCCTCAGCGAGATCAAGGTGCAGGAAGGTGAGCAGGTCGAGTCCGAAGC
CGTGCTCGCGGTCTTTGCCGAAGGCGCCGGCGGCGAGGCGAGCGGCGGTGACGCCGCCGCTTCGAAGGATGCGAGCGAAC
CCGCTGCGGCAGACGGCGGCAGCGACGAAAAGGTCGGCGACAAGATCCTGGCGCCCGCGGCACGCAAGCTGGTGGCCGAG
CATGATCTGGACGTCAACAAGATCGAAGGCACTGGCAAGGGCGGTCGCATCCTGAAAGAGGACGTGCAGAAAGCCGTCAA
GGACGGCTCCGCCAAGAAGAGCGCCAAGCCGTCAGGCGGTGACGTCAAGGCCGCTGCTGCGCCGGCGCCGGCCGTCGAGG
GCGAGCGCCCCGAGCAGCGTGTGCCGATGAGCCGTCTGCGCCAGACCATTGCCAAGCGCCTGGTCCAGGCACAGCAAACG
GCCGCCATGCTCACCACCTACAATGAGGTGGACATGAGCGCGGTGATGGCGCTGCGCAGTCAGTACAAGGACAGCTTCCA
GAAGGCGCACGACGTCAAGCTCGGCTTCATGGGCTTCTTCGTCAAGGCGGCGACCGAGGCGCTCAAGCGCTTCCCGGACG
TCAACGCTTCCATCGACGGCACCGACATTGTCTACCACGGCTACCAGGACATCGGTGTCGCCGTTTCCACGCCGCGTGGT
CTGGTGGTACCGGTGCTTCGCGATACCGACAGCATGAAGCTGGCCGATGTCGAGAAAACGATCGGTGACTTCGGCCAGCG
TGGCCGTGAAGGCAAGCTCGGCATCGAAGACATGCAGGGCGGGACCTTCACCATCACCAATGGCGGTATCTTCGGCTCGC
TGATGTCGACGCCGATCCTGAACCCGCCGCAGACCGCGATTCTGGGGATGCACAAGATTCAGGAACGCCCGATGGCCGTG
AACGGGAAGGTCGAGATCCGTCCGATGATGTATCTCGCCGTCTCCTACGACCACCGCATGATCGACGGCAAGGATGCGGT
ACAGTTCCTGGTCACGATCAAGGAATTGCTGGAAGATCCCGCGCGCTTCTTGCTCGACGTGTGA

Upstream 100 bases:

>100_bases
ATATGTCCGTCCATGTTGAACAGCAGCGCCAGCTGGTAGAAGACGCCATTACTGGGTGAGGCGTTTCATCGGGATCGAGA
GAAAACACTAAGGAAACGAC

Downstream 100 bases:

>100_bases
TGGCCGTCGCCGAATGATGCGTGCGACTTTTAAACTGAATGCGAACAGGATGATTTCATGGCCGATAAATTCGATGTGAT
CGTTATTGGCGCCGGGCCTG

Product: 2-oxoglutarate dehydrogenase E2 component

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MATEIKAPNFPESVAEGTIATWHKKVGDSVERDELIVEIETDKVVLEVVAPEAGTVTEIKVEEGDTCDSEQVLGMLGAAS
EGSNDKAASQESAEKSDDSGQDTAPAKADAKPAASGKQHDVKAPSFPESVQEGTVATWHKQVGEAVKRDEILADIETDKV
VLEVVAPADGALSEIKVQEGEQVESEAVLAVFAEGAGGEASGGDAAASKDASEPAAADGGSDEKVGDKILAPAARKLVAE
HDLDVNKIEGTGKGGRILKEDVQKAVKDGSAKKSAKPSGGDVKAAAAPAPAVEGERPEQRVPMSRLRQTIAKRLVQAQQT
AAMLTTYNEVDMSAVMALRSQYKDSFQKAHDVKLGFMGFFVKAATEALKRFPDVNASIDGTDIVYHGYQDIGVAVSTPRG
LVVPVLRDTDSMKLADVEKTIGDFGQRGREGKLGIEDMQGGTFTITNGGIFGSLMSTPILNPPQTAILGMHKIQERPMAV
NGKVEIRPMMYLAVSYDHRMIDGKDAVQFLVTIKELLEDPARFLLDV

Sequences:

>Translated_527_residues
MATEIKAPNFPESVAEGTIATWHKKVGDSVERDELIVEIETDKVVLEVVAPEAGTVTEIKVEEGDTCDSEQVLGMLGAAS
EGSNDKAASQESAEKSDDSGQDTAPAKADAKPAASGKQHDVKAPSFPESVQEGTVATWHKQVGEAVKRDEILADIETDKV
VLEVVAPADGALSEIKVQEGEQVESEAVLAVFAEGAGGEASGGDAAASKDASEPAAADGGSDEKVGDKILAPAARKLVAE
HDLDVNKIEGTGKGGRILKEDVQKAVKDGSAKKSAKPSGGDVKAAAAPAPAVEGERPEQRVPMSRLRQTIAKRLVQAQQT
AAMLTTYNEVDMSAVMALRSQYKDSFQKAHDVKLGFMGFFVKAATEALKRFPDVNASIDGTDIVYHGYQDIGVAVSTPRG
LVVPVLRDTDSMKLADVEKTIGDFGQRGREGKLGIEDMQGGTFTITNGGIFGSLMSTPILNPPQTAILGMHKIQERPMAV
NGKVEIRPMMYLAVSYDHRMIDGKDAVQFLVTIKELLEDPARFLLDV
>Mature_526_residues
ATEIKAPNFPESVAEGTIATWHKKVGDSVERDELIVEIETDKVVLEVVAPEAGTVTEIKVEEGDTCDSEQVLGMLGAASE
GSNDKAASQESAEKSDDSGQDTAPAKADAKPAASGKQHDVKAPSFPESVQEGTVATWHKQVGEAVKRDEILADIETDKVV
LEVVAPADGALSEIKVQEGEQVESEAVLAVFAEGAGGEASGGDAAASKDASEPAAADGGSDEKVGDKILAPAARKLVAEH
DLDVNKIEGTGKGGRILKEDVQKAVKDGSAKKSAKPSGGDVKAAAAPAPAVEGERPEQRVPMSRLRQTIAKRLVQAQQTA
AMLTTYNEVDMSAVMALRSQYKDSFQKAHDVKLGFMGFFVKAATEALKRFPDVNASIDGTDIVYHGYQDIGVAVSTPRGL
VVPVLRDTDSMKLADVEKTIGDFGQRGREGKLGIEDMQGGTFTITNGGIFGSLMSTPILNPPQTAILGMHKIQERPMAVN
GKVEIRPMMYLAVSYDHRMIDGKDAVQFLVTIKELLEDPARFLLDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=253, Percent_Identity=56.9169960474308, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI31711992, Length=566, Percent_Identity=28.0918727915194, Blast_Score=196, Evalue=5e-50,
Organism=Homo sapiens, GI203098816, Length=448, Percent_Identity=28.7946428571429, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI203098753, Length=448, Percent_Identity=28.7946428571429, Blast_Score=169, Evalue=6e-42,
Organism=Homo sapiens, GI110671329, Length=448, Percent_Identity=27.6785714285714, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=34.1614906832298, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=410, Percent_Identity=57.8048780487805, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1786305, Length=524, Percent_Identity=31.1068702290076, Blast_Score=200, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI25146366, Length=413, Percent_Identity=44.0677966101695, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=31.0267857142857, Blast_Score=193, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=413, Percent_Identity=26.634382566586, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=302, Percent_Identity=32.7814569536424, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6320352, Length=418, Percent_Identity=43.7799043062201, Blast_Score=329, Evalue=9e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=28.0087527352298, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI24645909, Length=230, Percent_Identity=59.1304347826087, Blast_Score=289, Evalue=4e-78,
Organism=Drosophila melanogaster, GI18859875, Length=416, Percent_Identity=28.8461538461538, Blast_Score=167, Evalue=2e-41,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 55832; Mature: 55701

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEIKAPNFPESVAEGTIATWHKKVGDSVERDELIVEIETDKVVLEVVAPEAGTVTEIK
CCCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCEEEEEEECCCCCCEEEEE
VEEGDTCDSEQVLGMLGAASEGSNDKAASQESAEKSDDSGQDTAPAKADAKPAASGKQHD
ECCCCCCCHHHHHHHHHCCCCCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VKAPSFPESVQEGTVATWHKQVGEAVKRDEILADIETDKVVLEVVAPADGALSEIKVQEG
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCC
EQVESEAVLAVFAEGAGGEASGGDAAASKDASEPAAADGGSDEKVGDKILAPAARKLVAE
CCHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HDLDVNKIEGTGKGGRILKEDVQKAVKDGSAKKSAKPSGGDVKAAAAPAPAVEGERPEQR
CCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHC
VPMSRLRQTIAKRLVQAQQTAAMLTTYNEVDMSAVMALRSQYKDSFQKAHDVKLGFMGFF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VKAATEALKRFPDVNASIDGTDIVYHGYQDIGVAVSTPRGLVVPVLRDTDSMKLADVEKT
HHHHHHHHHHCCCCCCCCCCCEEEEECCHHCCEEEECCCCEEEEEECCCCCCCHHHHHHH
IGDFGQRGREGKLGIEDMQGGTFTITNGGIFGSLMSTPILNPPQTAILGMHKIQERPMAV
HHHHHHCCCCCCCCCEECCCCEEEEECCCEEHHHHHCCCCCCCHHHHHHHHHHHCCCCEE
NGKVEIRPMMYLAVSYDHRMIDGKDAVQFLVTIKELLEDPARFLLDV
CCEEEEEEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATEIKAPNFPESVAEGTIATWHKKVGDSVERDELIVEIETDKVVLEVVAPEAGTVTEIK
CCCCCCCCCCHHHHCCCHHHHHHHHCCCCCCCCEEEEEECCEEEEEEECCCCCCEEEEE
VEEGDTCDSEQVLGMLGAASEGSNDKAASQESAEKSDDSGQDTAPAKADAKPAASGKQHD
ECCCCCCCHHHHHHHHHCCCCCCCCCHHCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
VKAPSFPESVQEGTVATWHKQVGEAVKRDEILADIETDKVVLEVVAPADGALSEIKVQEG
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHCCCCC
EQVESEAVLAVFAEGAGGEASGGDAAASKDASEPAAADGGSDEKVGDKILAPAARKLVAE
CCHHHCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HDLDVNKIEGTGKGGRILKEDVQKAVKDGSAKKSAKPSGGDVKAAAAPAPAVEGERPEQR
CCCCCEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHC
VPMSRLRQTIAKRLVQAQQTAAMLTTYNEVDMSAVMALRSQYKDSFQKAHDVKLGFMGFF
CCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VKAATEALKRFPDVNASIDGTDIVYHGYQDIGVAVSTPRGLVVPVLRDTDSMKLADVEKT
HHHHHHHHHHCCCCCCCCCCCEEEEECCHHCCEEEECCCCEEEEEECCCCCCCHHHHHHH
IGDFGQRGREGKLGIEDMQGGTFTITNGGIFGSLMSTPILNPPQTAILGMHKIQERPMAV
HHHHHHCCCCCCCCCEECCCCEEEEECCCEEHHHHHCCCCCCCHHHHHHHHHHHCCCCEE
NGKVEIRPMMYLAVSYDHRMIDGKDAVQFLVTIKELLEDPARFLLDV
CCEEEEEEEEEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]