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The map label for this gene is gcvA [H]

Identifier: 92113315

GI number: 92113315

Start: 1363066

End: 1363977

Strand: Direct

Name: gcvA [H]

Synonym: Csal_1188

Alternate gene names: 92113315

Gene position: 1363066-1363977 (Clockwise)

Preceding gene: 92113314

Following gene: 92113316

Centisome position: 36.87

GC content: 70.83

Gene sequence:

>912_bases
ATGCGCCGATTGCCCTCGTTGACCGCGCTGCGCACCTTCGAGTGCGCCGCCCGCCATGCCCACTTCGGCCGCGCGGCGGC
GGAGCTGTGCGTCACCGACAGCGCCGTCAGCCATCAGGTCCGCCAGTTGGAAGAGCAGCTCGGGGCGACGCTGTTCGTGC
GCCGCGGGCGCCACCTGCAGCCCACGCCTCAGGCCCGACGCCTGCTGCACAGCCTGCAGCAGGCCTTCGAACGAGTCGGC
GAGGCCTGCGAGACGATCGCCGCCCCGGATACCCAGGCCGCGCTGCAAGTGGCTGTGCCCGCCGAACTGGCTCAGAAGTG
GCTGGTGGGACGGCTGGACGACTTCTGCCGTCGCTACCCGCATATCGACCTGCATCTGCATGCCCGCGCCTACGACGTCC
AGGACGTCGGCCCCGACCTCGACCTGGCGATCATCTACGGCGCCGGACCGGCGGACGCCCATGAGCTTTTCGTGCGTCCC
TTGCCGGCGCTGCACTTCTTCCCGGTGTGCAGCCCGGGGCTGTTCAACGCGGGTGGCCTGACGTGTCCCGCCGACCTGGC
GGGTTGCCGATTGCTGCACGACGACCAGGACGGCGGAACCTGGGCCGCCTGGCTGGCCACCCACGCCGGCGACGCCCGGG
CCGGTCGCAACCTTTACTTCGAGCACGCCGGCCTGGCCCTGGAAGCCGCCGCGAACGGCCTGGGGGTGGCGCTCGGGGAT
GTGCTGACGGCCTCGGAGGACCTGACGCGGGGGCGGCTGGTGCGCCCCTTCGCCGCCAGCGTGGGTTCGCTCGGCCAGTA
CGCGCTGGTCTGCGATCGTCAGCGTCTCGAGCGGCCGGCGGTCGCGCACTTCATCGACTGGTGCGTCGCGCTGCTCGAGA
CTCCTGAATTCCATTCAAGGGTGGCGCAATAA

Upstream 100 bases:

>100_bases
GCCGGCCCTCGGGCCGGTCTTGTGAAGGGCGCGTCCGGCGAGGGGGGATCGCGTATCCTGTCGTCCAACCGTCGAGAGAT
CGCCCCAGGAGACTCCCGCC

Downstream 100 bases:

>100_bases
TCGTCGCTGGTGCGTCGCGCGAGGCCGGGCCTAGCATCGGAATACCCCAACAACGAAAACGAGGTGTTCCATGGCGCGCT
TGCTGACCTCCCTGCCCAAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MRRLPSLTALRTFECAARHAHFGRAAAELCVTDSAVSHQVRQLEEQLGATLFVRRGRHLQPTPQARRLLHSLQQAFERVG
EACETIAAPDTQAALQVAVPAELAQKWLVGRLDDFCRRYPHIDLHLHARAYDVQDVGPDLDLAIIYGAGPADAHELFVRP
LPALHFFPVCSPGLFNAGGLTCPADLAGCRLLHDDQDGGTWAAWLATHAGDARAGRNLYFEHAGLALEAAANGLGVALGD
VLTASEDLTRGRLVRPFAASVGSLGQYALVCDRQRLERPAVAHFIDWCVALLETPEFHSRVAQ

Sequences:

>Translated_303_residues
MRRLPSLTALRTFECAARHAHFGRAAAELCVTDSAVSHQVRQLEEQLGATLFVRRGRHLQPTPQARRLLHSLQQAFERVG
EACETIAAPDTQAALQVAVPAELAQKWLVGRLDDFCRRYPHIDLHLHARAYDVQDVGPDLDLAIIYGAGPADAHELFVRP
LPALHFFPVCSPGLFNAGGLTCPADLAGCRLLHDDQDGGTWAAWLATHAGDARAGRNLYFEHAGLALEAAANGLGVALGD
VLTASEDLTRGRLVRPFAASVGSLGQYALVCDRQRLERPAVAHFIDWCVALLETPEFHSRVAQ
>Mature_303_residues
MRRLPSLTALRTFECAARHAHFGRAAAELCVTDSAVSHQVRQLEEQLGATLFVRRGRHLQPTPQARRLLHSLQQAFERVG
EACETIAAPDTQAALQVAVPAELAQKWLVGRLDDFCRRYPHIDLHLHARAYDVQDVGPDLDLAIIYGAGPADAHELFVRP
LPALHFFPVCSPGLFNAGGLTCPADLAGCRLLHDDQDGGTWAAWLATHAGDARAGRNLYFEHAGLALEAAANGLGVALGD
VLTASEDLTRGRLVRPFAASVGSLGQYALVCDRQRLERPAVAHFIDWCVALLETPEFHSRVAQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=38.013698630137, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788706, Length=295, Percent_Identity=33.2203389830508, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=31.8181818181818, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI145693193, Length=294, Percent_Identity=24.4897959183673, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI157672245, Length=157, Percent_Identity=31.8471337579618, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1790262, Length=161, Percent_Identity=27.3291925465839, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI87081978, Length=263, Percent_Identity=25.0950570342205, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32977; Mature: 32977

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS50931 HTH_LYSR ; PS00290 IG_MHC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLPSLTALRTFECAARHAHFGRAAAELCVTDSAVSHQVRQLEEQLGATLFVRRGRHLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCC
PTPQARRLLHSLQQAFERVGEACETIAAPDTQAALQVAVPAELAQKWLVGRLDDFCRRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCHHHHHHHHHHHHHHHHHHCC
HIDLHLHARAYDVQDVGPDLDLAIIYGAGPADAHELFVRPLPALHFFPVCSPGLFNAGGL
CCEEEEEEEEEEHHHCCCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCCCCCCCCCCCCC
TCPADLAGCRLLHDDQDGGTWAAWLATHAGDARAGRNLYFEHAGLALEAAANGLGVALGD
CCCHHHHCCEEEECCCCCCEEHHHHHHCCCCCCCCCCEEEHHCCEEHHHHCCCCHHHHHH
VLTASEDLTRGRLVRPFAASVGSLGQYALVCDRQRLERPAVAHFIDWCVALLETPEFHSR
HHHCCHHHHCCCCHHHHHHHHCCCCCHHHHHCHHHHCCHHHHHHHHHHHHHHCCCHHHHH
VAQ
HCC
>Mature Secondary Structure
MRRLPSLTALRTFECAARHAHFGRAAAELCVTDSAVSHQVRQLEEQLGATLFVRRGRHLQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCC
PTPQARRLLHSLQQAFERVGEACETIAAPDTQAALQVAVPAELAQKWLVGRLDDFCRRYP
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCHHHHHHHHHHHHHHHHHHCC
HIDLHLHARAYDVQDVGPDLDLAIIYGAGPADAHELFVRPLPALHFFPVCSPGLFNAGGL
CCEEEEEEEEEEHHHCCCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCCCCCCCCCCCCC
TCPADLAGCRLLHDDQDGGTWAAWLATHAGDARAGRNLYFEHAGLALEAAANGLGVALGD
CCCHHHHCCEEEECCCCCCEEHHHHHHCCCCCCCCCCEEEHHCCEEHHHHCCCCHHHHHH
VLTASEDLTRGRLVRPFAASVGSLGQYALVCDRQRLERPAVAHFIDWCVALLETPEFHSR
HHHCCHHHHCCCCHHHHHHHHCCCCCHHHHHCHHHHCCHHHHHHHHHHHHHHCCCHHHHH
VAQ
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]