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The map label for this gene is proP [H]

Identifier: 92113286

GI number: 92113286

Start: 1325439

End: 1326746

Strand: Direct

Name: proP [H]

Synonym: Csal_1159

Alternate gene names: 92113286

Gene position: 1325439-1326746 (Clockwise)

Preceding gene: 92113285

Following gene: 92113295

Centisome position: 35.86

GC content: 63.61

Gene sequence:

>1308_bases
ATGGCTACTTCGCAAGAGACCGACGCGAGTGCGTCGCGCCCGCTGAATCGTCGCGACGTCAAGGTGCTGTCGCTGTCCGC
GCTCGGCGGCGCCCTGGAATTCTACGACTTCATCATTTTCGTGTACTTCGCGACGGTGGTGGGGCAACTGTTCTTTCCCC
CTGAAATGCCCGAATGGCTGCGTCAGATCCAGACCTTCGGGATCTTCGCCGCCGGCTACCTGGCACGCCCGCTGGGCGGC
ATCATCATGGCGCATTTCGGCGATCTGCTGGGGCGCAAGAAGATGTTCACCCTGTCGATCTTCCTGATGTCGGTGCCGAC
GCTGCTGATCGGCGTGATGCCCACCTACGACACCCTGGGGTATGCGGCGCCCTTGCTGCTGGTGGCGCTGCGCATCCTGC
AAGGGGCCGCCGTAGGCGGTGAAGTCCCCGGCGCCTGGGTGTTCGTCACCGAGCACGTCAAGCGCCACCATGTGGGCTTC
GCCTGCGGCACGCTGTCCGCCGGCCTGGTATCGGGCATTCTCATCGGCTCGCTGATGTCGGCGTTCATCAAGACCACCTA
CAGTGATGCGGAGCTGGCCGCGTATGCCTGGCGGATTCCGTTCTTGATCGGCGGGGTGTTCGGATTGCTGGCCGTGTATC
TGCGGCGCTGGCTGCACGAGACGCCGGTCTTCGCCGAAATGCAGCAGAAAAAGGCGCTGGCCGAGGAACTGCCGGTCAAG
AGCGTCTTGCGCAACCACTTGCCGAGCGTGGTGCTGTCGATGGGCGTGACCTGGATTCTCACCGCCGCCATCGTGGTGGT
GATCCTGATGACGCCGAGTCTGCTCGAGACGCGTTATGGCCTGGATGCCTCGCTGGCCAATGTGTATGCCATTCTCGGTG
TCGTGGTGGGGAGTCTGGCTTCCGGCTGGTGCGCGGATCGCCTCGGCAGCGGCCCGACCATCGCCTTTTGGGGCGTGCTG
CTGGCCATCAGCTACTGGGTGATGATGACCACCGTGAGCACGCACCCCGAATGGCTGACGCCGCTCTATATCCTGAGCGG
CTTCGCGGTGGGGATCGTCGGTGTGGTACCCACCATCGCCGTCAAGTCGTTTCCGGCGGTGGTGCGTTTCACGGGACTGT
CGTTTTCCTATAACGTCGCCTACGCCATCTTCGGTGGCTTCACGCCCATCGTGGTGTCGGTATTGATGACCGTGCATCCG
CTGTTCCCTGCGGTCTACGTCGCCGCGCTGGGCGGGCTGGGCGTGATCATCGGCGTGTACTTGATGCAAACGTCCAGCGG
GCGCCGACTGGCGGTCATGCCATCGTGA

Upstream 100 bases:

>100_bases
GCGGTCGTCGGCCGGTACCTCCCGCCTTGTTTGGGTGCCGCGACGCTGGACGCTGACATAACACGATCACCGATCATTCG
AACGGAAGACCACATCCATC

Downstream 100 bases:

>100_bases
CGCATTGACGCGCGACCTCGCCATGCCACGACGCCCCGCCGATGTGCGGGGCGTCGTCTTTCGGGCTCTTCTAGGAGCGT
CGCGCGGCGTCGACGAGGCC

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MATSQETDASASRPLNRRDVKVLSLSALGGALEFYDFIIFVYFATVVGQLFFPPEMPEWLRQIQTFGIFAAGYLARPLGG
IIMAHFGDLLGRKKMFTLSIFLMSVPTLLIGVMPTYDTLGYAAPLLLVALRILQGAAVGGEVPGAWVFVTEHVKRHHVGF
ACGTLSAGLVSGILIGSLMSAFIKTTYSDAELAAYAWRIPFLIGGVFGLLAVYLRRWLHETPVFAEMQQKKALAEELPVK
SVLRNHLPSVVLSMGVTWILTAAIVVVILMTPSLLETRYGLDASLANVYAILGVVVGSLASGWCADRLGSGPTIAFWGVL
LAISYWVMMTTVSTHPEWLTPLYILSGFAVGIVGVVPTIAVKSFPAVVRFTGLSFSYNVAYAIFGGFTPIVVSVLMTVHP
LFPAVYVAALGGLGVIIGVYLMQTSSGRRLAVMPS

Sequences:

>Translated_435_residues
MATSQETDASASRPLNRRDVKVLSLSALGGALEFYDFIIFVYFATVVGQLFFPPEMPEWLRQIQTFGIFAAGYLARPLGG
IIMAHFGDLLGRKKMFTLSIFLMSVPTLLIGVMPTYDTLGYAAPLLLVALRILQGAAVGGEVPGAWVFVTEHVKRHHVGF
ACGTLSAGLVSGILIGSLMSAFIKTTYSDAELAAYAWRIPFLIGGVFGLLAVYLRRWLHETPVFAEMQQKKALAEELPVK
SVLRNHLPSVVLSMGVTWILTAAIVVVILMTPSLLETRYGLDASLANVYAILGVVVGSLASGWCADRLGSGPTIAFWGVL
LAISYWVMMTTVSTHPEWLTPLYILSGFAVGIVGVVPTIAVKSFPAVVRFTGLSFSYNVAYAIFGGFTPIVVSVLMTVHP
LFPAVYVAALGGLGVIIGVYLMQTSSGRRLAVMPS
>Mature_434_residues
ATSQETDASASRPLNRRDVKVLSLSALGGALEFYDFIIFVYFATVVGQLFFPPEMPEWLRQIQTFGIFAAGYLARPLGGI
IMAHFGDLLGRKKMFTLSIFLMSVPTLLIGVMPTYDTLGYAAPLLLVALRILQGAAVGGEVPGAWVFVTEHVKRHHVGFA
CGTLSAGLVSGILIGSLMSAFIKTTYSDAELAAYAWRIPFLIGGVFGLLAVYLRRWLHETPVFAEMQQKKALAEELPVKS
VLRNHLPSVVLSMGVTWILTAAIVVVILMTPSLLETRYGLDASLANVYAILGVVVGSLASGWCADRLGSGPTIAFWGVLL
AISYWVMMTTVSTHPEWLTPLYILSGFAVGIVGVVPTIAVKSFPAVVRFTGLSFSYNVAYAIFGGFTPIVVSVLMTVHPL
FPAVYVAALGGLGVIIGVYLMQTSSGRRLAVMPS

Specific function: May be a proton symporter invoved in the uptake of osmolytes such as proline and glycine betaine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=439, Percent_Identity=27.7904328018223, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI1788292, Length=406, Percent_Identity=31.5270935960591, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1788942, Length=414, Percent_Identity=29.2270531400966, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789941, Length=435, Percent_Identity=29.1954022988506, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87082231, Length=441, Percent_Identity=23.3560090702948, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082404, Length=374, Percent_Identity=24.5989304812834, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46778; Mature: 46647

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHCCCCCCEEEECCC
>Mature Secondary Structure 
ATSQETDASASRPLNRRDVKVLSLSALGGALEFYDFIIFVYFATVVGQLFFPPEMPEWL
CCCCCCCCHHCCCCCCCCHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH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HHCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA