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The map label for this gene is nfi [H]

Identifier: 92113278

GI number: 92113278

Start: 1318932

End: 1319645

Strand: Direct

Name: nfi [H]

Synonym: Csal_1151

Alternate gene names: 92113278

Gene position: 1318932-1319645 (Clockwise)

Preceding gene: 92113275

Following gene: 92113280

Centisome position: 35.68

GC content: 69.19

Gene sequence:

>714_bases
ATGGCCAGGCAGGCCGCATTGCATGAGTGGAGTCTTTCCGCCGAGGAGGCCGTGGCGTTGCAGCGTCGCCTGGCGCCGCG
CGTGGTCAGGCACGGCAGACCTCAAGCGGTGAGCTGCATCGCGGGCGTGGATATCGGCTTCGAGGACGATGGCGAGACCA
CGCGCGCGGCGGTGGTGGTGCTCGACTGGCCGTCGCTTGCGGTGTGCGAGTCGGTGGTTCACCGCGAACCCACGCGGATG
CCCTACATTCCCGGCTTGCTGTCGTTCCGCGAGATTCCCGCCGCGCTGGGCGCCTTCGAACGACTGGCAGCACATCCCGA
TCTGGTCATGGTCGACGGTCAGGGCATCGCGCATCCCAGGCGGCTCGGCGTGGCGTCGCACCTGGGCTTGTGGCTGGACG
TGCCGACCCTCGGCGTGGCCAAGAAGGTGCTTTGCGGTCGTCATGGCGAAGTGCCGACCACGCGCGGTGCATGGACGCCG
CTCACCGACCGTCGGCGTGCCGAGGACCCCGCCGACGTCGAGGTCGACGCCGAAGGGCGCGTGGTCATCGGCGCGATGCT
GCGTTCCCGCGAGAACGTCAAGCCGGTGATCGTCTCTCCCGGGCATCGGCTGGATCAGGCCACGGCGCTGGAATGGGCGA
TTGCCTGCCTGGGCAGGACCAAGCTGCCCGAACCGACCCGGCTGGCGGATCGCCTGGCCTCGCGAAGAGGTTAG

Upstream 100 bases:

>100_bases
CTCCTATACCGGCCCTTCAGTCTACCGGTTGACGCGGAACGGCCCAATGGCCGTGCCAAGGGGCCGGGCCGGGAGGCGTT
CAAGACAAGGAGGAAGCGTT

Downstream 100 bases:

>100_bases
GTCGTCAGTGCCAGCCAGTGCTCGCCGAGCCAGCGTCCGGCGCGCTCGATCTCGGCTTCCGGGGCACCGGCGTAGCCGAG
AACGAGGCCTGGCCGGCCGG

Product: endonuclease V

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MARQAALHEWSLSAEEAVALQRRLAPRVVRHGRPQAVSCIAGVDIGFEDDGETTRAAVVVLDWPSLAVCESVVHREPTRM
PYIPGLLSFREIPAALGAFERLAAHPDLVMVDGQGIAHPRRLGVASHLGLWLDVPTLGVAKKVLCGRHGEVPTTRGAWTP
LTDRRRAEDPADVEVDAEGRVVIGAMLRSRENVKPVIVSPGHRLDQATALEWAIACLGRTKLPEPTRLADRLASRRG

Sequences:

>Translated_237_residues
MARQAALHEWSLSAEEAVALQRRLAPRVVRHGRPQAVSCIAGVDIGFEDDGETTRAAVVVLDWPSLAVCESVVHREPTRM
PYIPGLLSFREIPAALGAFERLAAHPDLVMVDGQGIAHPRRLGVASHLGLWLDVPTLGVAKKVLCGRHGEVPTTRGAWTP
LTDRRRAEDPADVEVDAEGRVVIGAMLRSRENVKPVIVSPGHRLDQATALEWAIACLGRTKLPEPTRLADRLASRRG
>Mature_236_residues
ARQAALHEWSLSAEEAVALQRRLAPRVVRHGRPQAVSCIAGVDIGFEDDGETTRAAVVVLDWPSLAVCESVVHREPTRMP
YIPGLLSFREIPAALGAFERLAAHPDLVMVDGQGIAHPRRLGVASHLGLWLDVPTLGVAKKVLCGRHGEVPTTRGAWTPL
TDRRRAEDPADVEVDAEGRVVIGAMLRSRENVKPVIVSPGHRLDQATALEWAIACLGRTKLPEPTRLADRLASRRG

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Homo sapiens, GI31542775, Length=208, Percent_Identity=33.1730769230769, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI257467548, Length=135, Percent_Identity=34.0740740740741, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI257467550, Length=135, Percent_Identity=34.0740740740741, Blast_Score=64, Evalue=8e-11,
Organism=Escherichia coli, GI87082357, Length=220, Percent_Identity=47.7272727272727, Blast_Score=187, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 25769; Mature: 25638

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQAALHEWSLSAEEAVALQRRLAPRVVRHGRPQAVSCIAGVDIGFEDDGETTRAAVVV
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEHEECCCCCCCCCCCCCEEEEEE
LDWPSLAVCESVVHREPTRMPYIPGLLSFREIPAALGAFERLAAHPDLVMVDGQGIAHPR
ECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHH
RLGVASHLGLWLDVPTLGVAKKVLCGRHGEVPTTRGAWTPLTDRRRAEDPADVEVDAEGR
HHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCC
VVIGAMLRSRENVKPVIVSPGHRLDQATALEWAIACLGRTKLPEPTRLADRLASRRG
EEEHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
ARQAALHEWSLSAEEAVALQRRLAPRVVRHGRPQAVSCIAGVDIGFEDDGETTRAAVVV
CCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEHEECCCCCCCCCCCCCEEEEEE
LDWPSLAVCESVVHREPTRMPYIPGLLSFREIPAALGAFERLAAHPDLVMVDGQGIAHPR
ECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHH
RLGVASHLGLWLDVPTLGVAKKVLCGRHGEVPTTRGAWTPLTDRRRAEDPADVEVDAEGR
HHHHHHHCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCC
VVIGAMLRSRENVKPVIVSPGHRLDQATALEWAIACLGRTKLPEPTRLADRLASRRG
EEEHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA