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The map label for this gene is mdlB [H]

Identifier: 92113203

GI number: 92113203

Start: 1214439

End: 1215638

Strand: Direct

Name: mdlB [H]

Synonym: Csal_1075

Alternate gene names: 92113203

Gene position: 1214439-1215638 (Clockwise)

Preceding gene: 92113202

Following gene: 92113204

Centisome position: 32.85

GC content: 64.75

Gene sequence:

>1200_bases
ATGAAAAGAAGCATGCCGCGCAGACGGCGCTACGATCAGGCACTGAGCATCGCGGAGCTTGCCGAGATCGCGCGCCGACG
GCTTCCGAACTTCGTCTATGAATACATTCATGGCGGCGCCGACGACGAAGTCAGCCTGCGCAACAACCGGGCCGTCTTCA
ACCGCTACCGGTTCACGCCCAAGACGCTGACCGATGTCAGTCAGCGGGATCTCGGCCGAGACCTGCTGGGCCACCGGGTG
AGCATGCCGGTCGTGATCGGGCCGACGGGGTTCAACGGCATGATCACCCAGGACGGAGACTCCAAATTGGCCCGCGCAGC
GGCGGACCGGGGGATTCCGTTCACGCTGAGCAATGCCTCGACCGAGCCGCTGGAAGAGATCGCCAAGGTCCCCGGTGGAT
GGCCATGGATGCAGATCTACTTCTATCGCGACCATGACTATGTGAAGAACCTGGTCGACCGCTGCCGGGCGTCGGGCTAC
GACACCATCGTCGTGACCACGGACAGCGCCATCTACGGCAACCGGGAGTGGGACACGCGCAATTACGCGCGCCCCTTCGT
GCTCAACTGGCGAAACAAGCTGCATGTGTTGAGCAGGCCCCGCTGGATGAAGGATGTGCTGTACCCGCATGGCGTGCCGA
CCTTCAAGAACCTGGGGGACCTCCTGCCGCCCGAGGATTCCTCCGTGCAGGGCGCGGCCGCCGAGATTGGCAAGCACTTG
ATGCCCTCCCTGAACTGGGAGGACATTCGCTGGCTGCGGGACAACTGGTCCGGCAACCTGCTGATCAAGGGCATCCTGTC
GGTGGAAGAAGCCCGCATGGCGGTGGAATACGGCATCGACGGCATCGTGCTGTCCAACCACGGCGGACGCCAACTGGACA
GCTCCGTCTCGCCGATGGAGATCCTCCCCGAGGTCAGGGCCGCCGTCGGCGATGCGCTCACTATCTTGCTGGACGGCGGC
TTCAGGCGCGGCAGCGACATTCTCAAGGCGGTGCTGCTGGGGGCGGACGCGGTGCTGCTCGGCCGCACGACCCTCTACGG
GCTCGGCGCGGGCGGCCAGGCGGGCGTCGAGCATGCACTTGGCTTACTGCACAAGGAGATGGACCGCACCCTGGGGCTTC
TCGGATGCAGCAACCTGCAGGAACTCGACCGCTCGTTGATACGCGATGCCTGCGCGCCGCAAACGGTTAGCCCCGCCTAG

Upstream 100 bases:

>100_bases
TGTTGATGTACGTGGCGATCCTGTTTGCACTGATTCTCTTCCCCGAGATCGTGACCTATCCCTTAAGTATTCTGCACAAC
GGTTGAATGATGGAGCTGAC

Downstream 100 bases:

>100_bases
GGCGGCAGGGTAGGATGGGCGGTAACAGAGCGTAATCTGCCGGGTCAGCCAGGAGCCAAAAGCATGATGAAAGGGGTGCA
GGAGCGGTCGTCGAGTGATC

Product: (S)-2-hydroxy-acid oxidase

Products: NA

Alternate protein names: L(+)-mandelate dehydrogenase; MDH [H]

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MKRSMPRRRRYDQALSIAELAEIARRRLPNFVYEYIHGGADDEVSLRNNRAVFNRYRFTPKTLTDVSQRDLGRDLLGHRV
SMPVVIGPTGFNGMITQDGDSKLARAAADRGIPFTLSNASTEPLEEIAKVPGGWPWMQIYFYRDHDYVKNLVDRCRASGY
DTIVVTTDSAIYGNREWDTRNYARPFVLNWRNKLHVLSRPRWMKDVLYPHGVPTFKNLGDLLPPEDSSVQGAAAEIGKHL
MPSLNWEDIRWLRDNWSGNLLIKGILSVEEARMAVEYGIDGIVLSNHGGRQLDSSVSPMEILPEVRAAVGDALTILLDGG
FRRGSDILKAVLLGADAVLLGRTTLYGLGAGGQAGVEHALGLLHKEMDRTLGLLGCSNLQELDRSLIRDACAPQTVSPA

Sequences:

>Translated_399_residues
MKRSMPRRRRYDQALSIAELAEIARRRLPNFVYEYIHGGADDEVSLRNNRAVFNRYRFTPKTLTDVSQRDLGRDLLGHRV
SMPVVIGPTGFNGMITQDGDSKLARAAADRGIPFTLSNASTEPLEEIAKVPGGWPWMQIYFYRDHDYVKNLVDRCRASGY
DTIVVTTDSAIYGNREWDTRNYARPFVLNWRNKLHVLSRPRWMKDVLYPHGVPTFKNLGDLLPPEDSSVQGAAAEIGKHL
MPSLNWEDIRWLRDNWSGNLLIKGILSVEEARMAVEYGIDGIVLSNHGGRQLDSSVSPMEILPEVRAAVGDALTILLDGG
FRRGSDILKAVLLGADAVLLGRTTLYGLGAGGQAGVEHALGLLHKEMDRTLGLLGCSNLQELDRSLIRDACAPQTVSPA
>Mature_399_residues
MKRSMPRRRRYDQALSIAELAEIARRRLPNFVYEYIHGGADDEVSLRNNRAVFNRYRFTPKTLTDVSQRDLGRDLLGHRV
SMPVVIGPTGFNGMITQDGDSKLARAAADRGIPFTLSNASTEPLEEIAKVPGGWPWMQIYFYRDHDYVKNLVDRCRASGY
DTIVVTTDSAIYGNREWDTRNYARPFVLNWRNKLHVLSRPRWMKDVLYPHGVPTFKNLGDLLPPEDSSVQGAAAEIGKHL
MPSLNWEDIRWLRDNWSGNLLIKGILSVEEARMAVEYGIDGIVLSNHGGRQLDSSVSPMEILPEVRAAVGDALTILLDGG
FRRGSDILKAVLLGADAVLLGRTTLYGLGAGGQAGVEHALGLLHKEMDRTLGLLGCSNLQELDRSLIRDACAPQTVSPA

Specific function: Reduction of (S)-mandelate to benzoylformate [H]

COG id: COG1304

COG function: function code C; L-lactate dehydrogenase (FMN-dependent) and related alpha-hydroxy acid dehydrogenases

Gene ontology:

Cell location: Membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FMN hydroxy acid dehydrogenase domain [H]

Homologues:

Organism=Homo sapiens, GI11068137, Length=377, Percent_Identity=37.9310344827586, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI54234014, Length=367, Percent_Identity=35.149863760218, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI7705393, Length=367, Percent_Identity=35.149863760218, Blast_Score=229, Evalue=4e-60,
Organism=Escherichia coli, GI1790033, Length=368, Percent_Identity=38.0434782608696, Blast_Score=246, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI193208036, Length=372, Percent_Identity=34.4086021505376, Blast_Score=214, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI32566217, Length=312, Percent_Identity=29.8076923076923, Blast_Score=145, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6323587, Length=387, Percent_Identity=32.5581395348837, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI281363140, Length=375, Percent_Identity=33.3333333333333, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI78707190, Length=375, Percent_Identity=33.3333333333333, Blast_Score=212, Evalue=3e-55,
Organism=Drosophila melanogaster, GI78707188, Length=375, Percent_Identity=33.3333333333333, Blast_Score=212, Evalue=3e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012133
- InterPro:   IPR000262
- InterPro:   IPR008259 [H]

Pfam domain/function: PF01070 FMN_dh [H]

EC number: =1.1.99.31 [H]

Molecular weight: Translated: 44405; Mature: 44405

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00557 FMN_HYDROXY_ACID_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSMPRRRRYDQALSIAELAEIARRRLPNFVYEYIHGGADDEVSLRNNRAVFNRYRFTP
CCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEECCCHHHHHHHCCCC
KTLTDVSQRDLGRDLLGHRVSMPVVIGPTGFNGMITQDGDSKLARAAADRGIPFTLSNAS
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEECCCHHHHHHHHHCCCCCEEECCCC
TEPLEEIAKVPGGWPWMQIYFYRDHDYVKNLVDRCRASGYDTIVVTTDSAIYGNREWDTR
CHHHHHHHCCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCC
NYARPFVLNWRNKLHVLSRPRWMKDVLYPHGVPTFKNLGDLLPPEDSSVQGAAAEIGKHL
CCCCCEEEECCCHHHHHCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHH
MPSLNWEDIRWLRDNWSGNLLIKGILSVEEARMAVEYGIDGIVLSNHGGRQLDSSVSPME
CCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHH
ILPEVRAAVGDALTILLDGGFRRGSDILKAVLLGADAVLLGRTTLYGLGAGGQAGVEHAL
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHHHHHH
GLLHKEMDRTLGLLGCSNLQELDRSLIRDACAPQTVSPA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKRSMPRRRRYDQALSIAELAEIARRRLPNFVYEYIHGGADDEVSLRNNRAVFNRYRFTP
CCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCEEECCCHHHHHHHCCCC
KTLTDVSQRDLGRDLLGHRVSMPVVIGPTGFNGMITQDGDSKLARAAADRGIPFTLSNAS
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCEECCCHHHHHHHHHCCCCCEEECCCC
TEPLEEIAKVPGGWPWMQIYFYRDHDYVKNLVDRCRASGYDTIVVTTDSAIYGNREWDTR
CHHHHHHHCCCCCCCEEEEEEEECHHHHHHHHHHHHCCCCCEEEEEECCEEECCCCCCCC
NYARPFVLNWRNKLHVLSRPRWMKDVLYPHGVPTFKNLGDLLPPEDSSVQGAAAEIGKHL
CCCCCEEEECCCHHHHHCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHH
MPSLNWEDIRWLRDNWSGNLLIKGILSVEEARMAVEYGIDGIVLSNHGGRQLDSSVSPME
CCCCCHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHH
ILPEVRAAVGDALTILLDGGFRRGSDILKAVLLGADAVLLGRTTLYGLGAGGQAGVEHAL
HHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCEEECCCEEEEECCCCHHHHHHHH
GLLHKEMDRTLGLLGCSNLQELDRSLIRDACAPQTVSPA
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2271624 [H]