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The map label for this gene is purU [H]

Identifier: 92113130

GI number: 92113130

Start: 1129873

End: 1130739

Strand: Direct

Name: purU [H]

Synonym: Csal_1002

Alternate gene names: 92113130

Gene position: 1129873-1130739 (Clockwise)

Preceding gene: 92113129

Following gene: 92113131

Centisome position: 30.56

GC content: 61.82

Gene sequence:

>867_bases
ATGAGGCGTAAAGATGCCACCTGGATCCTCACCGCTCAGTGCCCCAGCCGCCTGGGGACTGTCGACGTGGTCACGCGTTT
CTTGAAGGAGTGTCGCTGCTACATCACCGAGCAGCAATCCTTCGATGACCGCCACGGTGAGCGCTTTTTCATCCGCACGG
CTTTCCAGCCCGAGGAGACGGATTTCGACGCCGATACCTTCCAGGCGGCGTTCGCCGCCCGCGCGGCCGAGTTCGACATG
GTCTTCGAGCTGACCGCGCCGGAGGCGCGCATGCCGGTGGTGATCATGGTGTCCAAGGCGGATCATTGCTTGAACGACCT
GCTATACCGCTATCGCACGGGGCAACTGCCGGTGACGATTCGTGCGGTGATTTCCAATCATCCGGATCTCGAGCCGCTGG
TCGCTTGGCACGATTTGCCTTATTACCACCTGCCGATCACGCCCGAGACCAAGGCCGAGCAGGAAGCCGAGGTGTGGCGA
GTGATCGAGTCCACCGGGGCGGAGCTGGTTATCCTGGCGCGTTACATGCAGGTGCTTTCCAGCGAGCTGTGCGAGAAACT
CACGGGCAAGGCGATCAACATCCACCATTCGCTGCTGCCAGGGTTCAAGGGCGCCAAACCTTACCATCAGGCGTTCGAGA
AAGGCGTCAAGCTGGTCGGTGCCACCGCGCACTACATCAACGACGATCTCGATGAGGGGCCGATCATCACCCAGGGCGTG
GAGCCTGTCAGCCATGCCGATGACCCCGAGGACCTGGTCGCCAAGGGACGCGATATCGAATGCTTGACCCTGGCGCGGGC
GGTATCGCTTCATCTGGAGCGACGGGTGTTTCTGCATGCCAGGCGTACCGTGGTCTTCGCGCGCTGA

Upstream 100 bases:

>100_bases
TGGGAAATCATCGTGCGGCGCAGTTTTGCCGACTACGTATACCGCTGGCTGCTCGATGCCGGCGAGGAGTATGGTATCGG
TGTCACACGAGAAAGCCATC

Downstream 100 bases:

>100_bases
CGGAGGCGAAAGCGGGAATTTTTCCAGCGTTCTCCTAATCTAAGGATGACGGGACGGCAAACGACAACACCGCCCAACGA
CGGCCGAGTACCCACAACCA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRRKDATWILTAQCPSRLGTVDVVTRFLKECRCYITEQQSFDDRHGERFFIRTAFQPEETDFDADTFQAAFAARAAEFDM
VFELTAPEARMPVVIMVSKADHCLNDLLYRYRTGQLPVTIRAVISNHPDLEPLVAWHDLPYYHLPITPETKAEQEAEVWR
VIESTGAELVILARYMQVLSSELCEKLTGKAINIHHSLLPGFKGAKPYHQAFEKGVKLVGATAHYINDDLDEGPIITQGV
EPVSHADDPEDLVAKGRDIECLTLARAVSLHLERRVFLHARRTVVFAR

Sequences:

>Translated_288_residues
MRRKDATWILTAQCPSRLGTVDVVTRFLKECRCYITEQQSFDDRHGERFFIRTAFQPEETDFDADTFQAAFAARAAEFDM
VFELTAPEARMPVVIMVSKADHCLNDLLYRYRTGQLPVTIRAVISNHPDLEPLVAWHDLPYYHLPITPETKAEQEAEVWR
VIESTGAELVILARYMQVLSSELCEKLTGKAINIHHSLLPGFKGAKPYHQAFEKGVKLVGATAHYINDDLDEGPIITQGV
EPVSHADDPEDLVAKGRDIECLTLARAVSLHLERRVFLHARRTVVFAR
>Mature_288_residues
MRRKDATWILTAQCPSRLGTVDVVTRFLKECRCYITEQQSFDDRHGERFFIRTAFQPEETDFDADTFQAAFAARAAEFDM
VFELTAPEARMPVVIMVSKADHCLNDLLYRYRTGQLPVTIRAVISNHPDLEPLVAWHDLPYYHLPITPETKAEQEAEVWR
VIESTGAELVILARYMQVLSSELCEKLTGKAINIHHSLLPGFKGAKPYHQAFEKGVKLVGATAHYINDDLDEGPIITQGV
EPVSHADDPEDLVAKGRDIECLTLARAVSLHLERRVFLHARRTVVFAR

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=177, Percent_Identity=32.2033898305085, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI209869995, Length=177, Percent_Identity=32.2033898305085, Blast_Score=82, Evalue=6e-16,
Organism=Homo sapiens, GI209869993, Length=177, Percent_Identity=32.2033898305085, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1787483, Length=278, Percent_Identity=40.6474820143885, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1788846, Length=196, Percent_Identity=28.0612244897959, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17567511, Length=156, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24582400, Length=116, Percent_Identity=33.6206896551724, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32702; Mature: 32702

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKDATWILTAQCPSRLGTVDVVTRFLKECRCYITEQQSFDDRHGERFFIRTAFQPEET
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCC
DFDADTFQAAFAARAAEFDMVFELTAPEARMPVVIMVSKADHCLNDLLYRYRTGQLPVTI
CCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEE
RAVISNHPDLEPLVAWHDLPYYHLPITPETKAEQEAEVWRVIESTGAELVILARYMQVLS
EEECCCCCCCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SELCEKLTGKAINIHHSLLPGFKGAKPYHQAFEKGVKLVGATAHYINDDLDEGPIITQGV
HHHHHHHCCCEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
EPVSHADDPEDLVAKGRDIECLTLARAVSLHLERRVFLHARRTVVFAR
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCEEEEEC
>Mature Secondary Structure
MRRKDATWILTAQCPSRLGTVDVVTRFLKECRCYITEQQSFDDRHGERFFIRTAFQPEET
CCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCC
DFDADTFQAAFAARAAEFDMVFELTAPEARMPVVIMVSKADHCLNDLLYRYRTGQLPVTI
CCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHCCCCCCEEE
RAVISNHPDLEPLVAWHDLPYYHLPITPETKAEQEAEVWRVIESTGAELVILARYMQVLS
EEECCCCCCCCHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SELCEKLTGKAINIHHSLLPGFKGAKPYHQAFEKGVKLVGATAHYINDDLDEGPIITQGV
HHHHHHHCCCEEEEHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCC
EPVSHADDPEDLVAKGRDIECLTLARAVSLHLERRVFLHARRTVVFAR
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7543100 [H]