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The map label for this gene is stcD [H]

Identifier: 92113118

GI number: 92113118

Start: 1110394

End: 1112478

Strand: Direct

Name: stcD [H]

Synonym: Csal_0990

Alternate gene names: 92113118

Gene position: 1110394-1112478 (Clockwise)

Preceding gene: 92113117

Following gene: 92113119

Centisome position: 30.04

GC content: 65.37

Gene sequence:

>2085_bases
ATGGCATTCGATGCGTTGTTCAAGCCGATCGAGATCGGCAATCTCACCATACGCAACCGCGTGGTCAGCACCGCCCATGC
CGAGGTTCACGCGACCGATGGCGGCATGACCACCGAACGTTACGTCAAGTACTACGAGGAGAAGGCCAAGGGCGGCTGCG
GATTGTGCATCTGCGGCGGCTCCTCGCCGGTATCGATCGACAGCCCCCAGGCCTGGTGGAGTTCGGTCAACATTTCCACC
GACCGGATCATTCCGCACTTCCAGAACCTGGCGGACGCCGTGCACAAGCATGGCGGCAAGATCATGATCCAGATCACCCA
CATGGGCCGCCGTTCGCGCTGGGACGGTTTCGACTGGCCGACACTGCTGTCGCCGTCGGGCATTCGCGAGCCGGTGCACC
GCTCGACTTGCAAGACCATCGAGGAAGAGGAGATCTGGCGCGTCATCGACGATTTCGCCCAGGGCGCGCGTCGCGTCAAG
GAGGGCGGCCTCGACGGCATCGAGCTGTCGGCCGTGCACCAGCACCTGATCGATCAGTTCTGGAGTCCGCGCGTCAACAA
GCGTGAAGACCAGTGGGGCGGCAGCTTCGAGAATCGCATGCGCTTCGGCCTGGAAGTCCTCAAGGCGGTACGCGCCGAAG
TCGGCGACGACTTCGTGGTCGGCCTGCGCATCTGCGGCGACGAGTTCCATCCCGACGGCCTGACCCACGACGACATGAAG
CGGATCGCCGCCTACTACGATGCCACCGGCATGGTCGATTTCTTCGGCGTGGTCGGCTCGGGATGCGACACGCACAATAC
CCTGGCCAACGTGATTCCCAACATGTCCTACCCGCCGGAGCCGTTCCTGCACCTGGCGGCGGGCGTCAAGGACGTGGTCA
GCGTGCCGGTGATCCATGCCCAGAACATCAAGGACCCCAACCAGGCCAGTCGTATTCTCGAGGGCGGCTACGTCGACCTG
GTGGGCATGACCCGCGCGCACATCGCCGACCCGCATCTGATCGCCAAGATCCAGATGGACCAGACCGACCGGATCCGGCA
GTGCGTGGGCGCCAACTACTGTATCGATCGCCAATACCAGGGGCTCGACGTGCTGTGTATCCAGAACGCCGCCACCTCGC
GCGAGTACCTGGGCCTGCCCCACGAGATCGCCCCCAGCGACGGGCCGAAGCGCAAGGTAGTGGTGGTCGGCGGCGGTCCG
GGCGGCATGGAGGCGGCGCGCGTCGCGGCCGAGCGCGGCCACGAGGTCACGCTGTTCGAGGCCGCGGACCAGATCGGCGG
TCAGATCACGCTCGCCGCCAAGGCACCTCAGCGCGACCAGATCGCCGGCATCACCCGCTGGTACCAGCTCGAGCTGGCAC
GACTCAAGGTCGACCTGCGCCTGGGTACCCGTGCCGACGAGGCGACCATCGCCGACCTGCGGCCGGACATCGTGGTGCTC
GCCACCGGCGGCCAACCCTTCCTGTCGCAAGTGCCCGAATGGGGCTATGACGAGGATCGCGAACGGAGCCTGGTCGTCAG
CACCTGGGACATTCTCAGCGGCGCCGTCGAGCCCGGCAAGAACGTGCTGATCTTCGATTCCATCTGCGAGTTCGCCGGCG
TCTCGGCGGCGGATTACCTGGCCGACAAGGGGGCGACGGTCGAGATCGTCACCGACGACATCAAGCCGGGCGCGGCGGTC
GGCGGCACCACCTTCCCGACCTATTACCGCAGCCTCTACGAGAAGGAGGTGATCATGACCTCCGACCTGGCGCTGCACAG
GGTCTATCGCGAGGGCGATGCCCTGGTCGCGGTACTCGAGAACGAGTACACCGGCGCCCAGGAAGAGCGGGTGGTCGACC
AGGTGGTGGTCGAAAATGGCGTGCGACCCGACGAGGCGCTCTATTACGCCCTCAAGGATCAGTCGCGCAACAAGGGGCAG
GTCGACATCGAAGCGCTCTATGCCATCCAGCCCCAGCCCGCGTTGGCGACGCTGGACGACGATGCCGATGCCGGCTTCGT
GCTGTTCCGACTGGGCGATTGCACGGCGCCGCGCAATACGCATGCGGCGATCTATGATGCCCTGCGGATCTGCAAGGACT
TCTGA

Upstream 100 bases:

>100_bases
ACCGTCGCCGAGCAGACCCAGAGCGCCGCCGAGGAAGGCTGCGACGTCGGCCGCTTCACGGTCGTTCCCCGTCATATCCT
GGCAGGCTGAGGTTACCGAG

Downstream 100 bases:

>100_bases
GGAAAGCTATTGCGCTCGGCCAGGCGTTGTTGAAGGTCGACTCGTCATGCTCATGGAACACCCAGTCCACTGCGCTGACT
CGTCGACCTTCGCCTAACCT

Product: NADH:flavin oxidoreductase/NADH oxidase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: Stachydrine utilization protein stcD [H]

Number of amino acids: Translated: 694; Mature: 693

Protein sequence:

>694_residues
MAFDALFKPIEIGNLTIRNRVVSTAHAEVHATDGGMTTERYVKYYEEKAKGGCGLCICGGSSPVSIDSPQAWWSSVNIST
DRIIPHFQNLADAVHKHGGKIMIQITHMGRRSRWDGFDWPTLLSPSGIREPVHRSTCKTIEEEEIWRVIDDFAQGARRVK
EGGLDGIELSAVHQHLIDQFWSPRVNKREDQWGGSFENRMRFGLEVLKAVRAEVGDDFVVGLRICGDEFHPDGLTHDDMK
RIAAYYDATGMVDFFGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGVKDVVSVPVIHAQNIKDPNQASRILEGGYVDL
VGMTRAHIADPHLIAKIQMDQTDRIRQCVGANYCIDRQYQGLDVLCIQNAATSREYLGLPHEIAPSDGPKRKVVVVGGGP
GGMEAARVAAERGHEVTLFEAADQIGGQITLAAKAPQRDQIAGITRWYQLELARLKVDLRLGTRADEATIADLRPDIVVL
ATGGQPFLSQVPEWGYDEDRERSLVVSTWDILSGAVEPGKNVLIFDSICEFAGVSAADYLADKGATVEIVTDDIKPGAAV
GGTTFPTYYRSLYEKEVIMTSDLALHRVYREGDALVAVLENEYTGAQEERVVDQVVVENGVRPDEALYYALKDQSRNKGQ
VDIEALYAIQPQPALATLDDDADAGFVLFRLGDCTAPRNTHAAIYDALRICKDF

Sequences:

>Translated_694_residues
MAFDALFKPIEIGNLTIRNRVVSTAHAEVHATDGGMTTERYVKYYEEKAKGGCGLCICGGSSPVSIDSPQAWWSSVNIST
DRIIPHFQNLADAVHKHGGKIMIQITHMGRRSRWDGFDWPTLLSPSGIREPVHRSTCKTIEEEEIWRVIDDFAQGARRVK
EGGLDGIELSAVHQHLIDQFWSPRVNKREDQWGGSFENRMRFGLEVLKAVRAEVGDDFVVGLRICGDEFHPDGLTHDDMK
RIAAYYDATGMVDFFGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGVKDVVSVPVIHAQNIKDPNQASRILEGGYVDL
VGMTRAHIADPHLIAKIQMDQTDRIRQCVGANYCIDRQYQGLDVLCIQNAATSREYLGLPHEIAPSDGPKRKVVVVGGGP
GGMEAARVAAERGHEVTLFEAADQIGGQITLAAKAPQRDQIAGITRWYQLELARLKVDLRLGTRADEATIADLRPDIVVL
ATGGQPFLSQVPEWGYDEDRERSLVVSTWDILSGAVEPGKNVLIFDSICEFAGVSAADYLADKGATVEIVTDDIKPGAAV
GGTTFPTYYRSLYEKEVIMTSDLALHRVYREGDALVAVLENEYTGAQEERVVDQVVVENGVRPDEALYYALKDQSRNKGQ
VDIEALYAIQPQPALATLDDDADAGFVLFRLGDCTAPRNTHAAIYDALRICKDF
>Mature_693_residues
AFDALFKPIEIGNLTIRNRVVSTAHAEVHATDGGMTTERYVKYYEEKAKGGCGLCICGGSSPVSIDSPQAWWSSVNISTD
RIIPHFQNLADAVHKHGGKIMIQITHMGRRSRWDGFDWPTLLSPSGIREPVHRSTCKTIEEEEIWRVIDDFAQGARRVKE
GGLDGIELSAVHQHLIDQFWSPRVNKREDQWGGSFENRMRFGLEVLKAVRAEVGDDFVVGLRICGDEFHPDGLTHDDMKR
IAAYYDATGMVDFFGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGVKDVVSVPVIHAQNIKDPNQASRILEGGYVDLV
GMTRAHIADPHLIAKIQMDQTDRIRQCVGANYCIDRQYQGLDVLCIQNAATSREYLGLPHEIAPSDGPKRKVVVVGGGPG
GMEAARVAAERGHEVTLFEAADQIGGQITLAAKAPQRDQIAGITRWYQLELARLKVDLRLGTRADEATIADLRPDIVVLA
TGGQPFLSQVPEWGYDEDRERSLVVSTWDILSGAVEPGKNVLIFDSICEFAGVSAADYLADKGATVEIVTDDIKPGAAVG
GTTFPTYYRSLYEKEVIMTSDLALHRVYREGDALVAVLENEYTGAQEERVVDQVVVENGVRPDEALYYALKDQSRNKGQV
DIEALYAIQPQPALATLDDDADAGFVLFRLGDCTAPRNTHAAIYDALRICKDF

Specific function: Possible NADH-dependent oxidase, functions as a demethylase that converts N-methylproline to proline [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789463, Length=679, Percent_Identity=30.0441826215022, Blast_Score=272, Evalue=6e-74,
Organism=Escherichia coli, GI1787939, Length=359, Percent_Identity=27.0194986072423, Blast_Score=102, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17565138, Length=383, Percent_Identity=24.2819843342037, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=269, Percent_Identity=30.8550185873606, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17564188, Length=188, Percent_Identity=34.0425531914894, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17559804, Length=273, Percent_Identity=28.5714285714286, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17540738, Length=267, Percent_Identity=26.9662921348315, Blast_Score=98, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=274, Percent_Identity=28.1021897810219, Blast_Score=96, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17566914, Length=250, Percent_Identity=26.4, Blast_Score=89, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321973, Length=241, Percent_Identity=29.4605809128631, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325086, Length=241, Percent_Identity=28.2157676348548, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 76345; Mature: 76214

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFDALFKPIEIGNLTIRNRVVSTAHAEVHATDGGMTTERYVKYYEEKAKGGCGLCICGG
CCHHHHCCCEECCCEEEHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECC
SSPVSIDSPQAWWSSVNISTDRIIPHFQNLADAVHKHGGKIMIQITHMGRRSRWDGFDWP
CCCCCCCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
TLLSPSGIREPVHRSTCKTIEEEEIWRVIDDFAQGARRVKEGGLDGIELSAVHQHLIDQF
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHH
WSPRVNKREDQWGGSFENRMRFGLEVLKAVRAEVGDDFVVGLRICGDEFHPDGLTHDDMK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHH
RIAAYYDATGMVDFFGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGVKDVVSVPVIHA
HHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHCCCEEE
QNIKDPNQASRILEGGYVDLVGMTRAHIADPHLIAKIQMDQTDRIRQCVGANYCIDRQYQ
CCCCCHHHHHHHHCCCCEEEEECHHHHCCCCCEEEEEECCCHHHHHHHHCCCCEECCCCC
GLDVLCIQNAATSREYLGLPHEIAPSDGPKRKVVVVGGGPGGMEAARVAAERGHEVTLFE
CEEEEEECCCCCCHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEE
AADQIGGQITLAAKAPQRDQIAGITRWYQLELARLKVDLRLGTRADEATIADLRPDIVVL
CHHHHCCEEEEEECCCCCHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCHHCCCCCEEEE
ATGGQPFLSQVPEWGYDEDRERSLVVSTWDILSGAVEPGKNVLIFDSICEFAGVSAADYL
ECCCCHHHHHCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCEEEEHHHHHHHCCCHHHHH
ADKGATVEIVTDDIKPGAAVGGTTFPTYYRSLYEKEVIMTSDLALHRVYREGDALVAVLE
HCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHEECHHHHHHHHHCCCEEEEEEE
NEYTGAQEERVVDQVVVENGVRPDEALYYALKDQSRNKGQVDIEALYAIQPQPALATLDD
CCCCCCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCCEEEEEEEEECCCCCEEEECC
DADAGFVLFRLGDCTAPRNTHAAIYDALRICKDF
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFDALFKPIEIGNLTIRNRVVSTAHAEVHATDGGMTTERYVKYYEEKAKGGCGLCICGG
CHHHHCCCEECCCEEEHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECC
SSPVSIDSPQAWWSSVNISTDRIIPHFQNLADAVHKHGGKIMIQITHMGRRSRWDGFDWP
CCCCCCCCCHHHHHCCCCCHHHCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCC
TLLSPSGIREPVHRSTCKTIEEEEIWRVIDDFAQGARRVKEGGLDGIELSAVHQHLIDQF
CCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHH
WSPRVNKREDQWGGSFENRMRFGLEVLKAVRAEVGDDFVVGLRICGDEFHPDGLTHDDMK
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHH
RIAAYYDATGMVDFFGVVGSGCDTHNTLANVIPNMSYPPEPFLHLAAGVKDVVSVPVIHA
HHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHCHHHHHHCCCEEE
QNIKDPNQASRILEGGYVDLVGMTRAHIADPHLIAKIQMDQTDRIRQCVGANYCIDRQYQ
CCCCCHHHHHHHHCCCCEEEEECHHHHCCCCCEEEEEECCCHHHHHHHHCCCCEECCCCC
GLDVLCIQNAATSREYLGLPHEIAPSDGPKRKVVVVGGGPGGMEAARVAAERGHEVTLFE
CEEEEEECCCCCCHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEE
AADQIGGQITLAAKAPQRDQIAGITRWYQLELARLKVDLRLGTRADEATIADLRPDIVVL
CHHHHCCEEEEEECCCCCHHHHHHHHHHEEEEEEEEEEEEECCCCCCCCHHCCCCCEEEE
ATGGQPFLSQVPEWGYDEDRERSLVVSTWDILSGAVEPGKNVLIFDSICEFAGVSAADYL
ECCCCHHHHHCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCEEEEHHHHHHHCCCHHHHH
ADKGATVEIVTDDIKPGAAVGGTTFPTYYRSLYEKEVIMTSDLALHRVYREGDALVAVLE
HCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHEECHHHHHHHHHCCCEEEEEEE
NEYTGAQEERVVDQVVVENGVRPDEALYYALKDQSRNKGQVDIEALYAIQPQPALATLDD
CCCCCCHHHHHHHHHHHHCCCCCCHHEEEEECCCCCCCCCEEEEEEEEECCCCCEEEECC
DADAGFVLFRLGDCTAPRNTHAAIYDALRICKDF
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9758825; 11481431 [H]