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The map label for this gene is rpoD [H]

Identifier: 92113104

GI number: 92113104

Start: 1093279

End: 1095123

Strand: Direct

Name: rpoD [H]

Synonym: Csal_0976

Alternate gene names: 92113104

Gene position: 1093279-1095123 (Clockwise)

Preceding gene: 92113103

Following gene: 92113106

Centisome position: 29.57

GC content: 62.93

Gene sequence:

>1845_bases
ATGGCTGGAAATACGCAGCAGTCACGTCTGAAGGAGTTGATCGCCCAGGGTAAGGAACAGGGCTTCCTGACCTATGCGGA
GGTCAACGACCACCTTCCCGAGGATATCGCCGATCCCGATCAGGTGGAAGACATCATCGCGATGATCAACGACATGGGTA
TCAGCGTCGTCGAGGAAGCGCCTGACGAAGATACCCTGATGATGTCCGATCAATCCGCCGACGAATCGGCGGCGGAAGAA
GCCGTCGCCGCGCTGGCCGCGGTGGAAAGCGATGTCGGCCGCACCACCGATCCCGTGCGCATGTACATGCGCGAGATGGG
TACGGTGGAATTGTTGACGCGCGAGGGCGAGATCGAAATCGCCAAGCGTATCGAGGAAGGCACCCGTGAGGTGATGTCGT
CGCTGGCCTATTTGCCTGGCGCCGTGGACTCCATTCTCAGCGCTTACGATGCCACCCAGGATGAAGAAGCGCCGGGCCGG
CTGTCCGACCTGTTTTCCGGCTTCATCGACCCCGACGAAGGGATTCCCGGCGTGGCCGAGGCGGAGGTGCCCGAGCCCGA
GCCCGAGCCGAGCGCCGCGGACGGCGATGTCTCCGAGGACGATGACGAGGCCGAGGAAGAGGAAACCGGTGGTGGTCCGG
ACCCCGAAGAAGCGCGGGCACGCTTCGAGCAGATCCGTGAGCAGAACGAGCGCGCCAAGGCCGCGATCGAAGAGCACGGG
CTCGGCTCGAAGGAAGCCAGCGCCGAGCAGGCCCGTCTGGCCGAGCTGTTCTCGCCGATCAAGCTGGTGCCCAAGCATTT
CGAGCGTCTGGTGGGCCAGGTGCGGATCAGCGTCGAGCAGATCCGCGGCCAGGAAAAGGCCATCATGCAGATCTTCGTGA
AGCAGGCCAAGGTGCCGCGCAAGACCTTCATCGGGTCGTTTCCGGGCGCCGAGTCCGACCTCGAGTGGCTGGACCGGTTC
ATGGCCAAGCACACCAAGTTCGCCGATCGTCTCGAGCCGTTCCGCGCCGACATCCAGCGTGCCCAGCGCAAGATCGGCTT
CGAGGAAGAGATGGTCCTGCTGCCGGTCTCGCACATCAAGGAAGTCAATCGCCGCCTGTCGATCGGCGAGGCCAAGGCGC
GTCGCGCCAAGAAGGAAATGGTCGAGGCCAACCTGCGTCTGGTCATCTCGATCGCCAAGAAGTACACCAACCGTGGCCTG
CAGTTCCTGGACCTCATCCAGGAAGGCAACATCGGCCTGATGAAGGCGGTGGACAAGTTCGAGTATCGTCGTGGCTACAA
GTTCTCGACCTATGCCACCTGGTGGATTCGTCAGGCGATCACGCGGTCGATCGCCGACCAGGCGCGCACCATCCGTATTC
CGGTGCACATGATCGAGACCATCAACAAGCTCAACCGCGTGTCGCGGCAGATGCTGCAGGAGATGGGGCGCGAGCCGACA
CCGGAAGAATTGGGCGAGCGTCTCGAGATGCCCGAGGACAAGGTGCGCAAGGTGCTCAAGATCGCCAAGGAACCGATCTC
CATGGAGACTCCGATCGGCGACGACGACGACTCGCATCTCGGCGATTTCATCGAGGATGGCACGATGCTGTTGCCGATCG
ACTCCGCGACCGGCGAAGGCTTGATCGAGGCGACCCGCAACGTGCTGGGCGGTCTGACCGCGCGCGAGGCCAAGGTGCTG
CGCATGCGCTTCGGCATCGACATGAACACCGACCACACGCTGGAAGAGGTCGGCAAGCAGTTCGATGTCACCCGCGAGCG
TATCCGTCAGATCGAGGCCAAGGCACTGCGCAAGTTGCGTCACCCCTCACGCTCCGAGCCGCTGCGCACCTTCCTCGACG
AGTAA

Upstream 100 bases:

>100_bases
CGACACTGGCAGTAAAATGCCCATAGCGGCTATACTGGCAGGCTTAGCAGCGTTTCGATCCGTTCACCTCCAGGTGCTCC
ATTGTCGAGATAGGGTTTCT

Downstream 100 bases:

>100_bases
GTCGGGCAAGGGCGCAAGCCGGATATGAAAAACCCGCGAGGTCCTCCTCGCGGGTTTTTTTGGTCGGTCGTGGCGTTGTC
GTCAGGCGGCGCGGGCGCGA

Product: sigma 70 (RpoD)

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 614; Mature: 613

Protein sequence:

>614_residues
MAGNTQQSRLKELIAQGKEQGFLTYAEVNDHLPEDIADPDQVEDIIAMINDMGISVVEEAPDEDTLMMSDQSADESAAEE
AVAALAAVESDVGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEEGTREVMSSLAYLPGAVDSILSAYDATQDEEAPGR
LSDLFSGFIDPDEGIPGVAEAEVPEPEPEPSAADGDVSEDDDEAEEEETGGGPDPEEARARFEQIREQNERAKAAIEEHG
LGSKEASAEQARLAELFSPIKLVPKHFERLVGQVRISVEQIRGQEKAIMQIFVKQAKVPRKTFIGSFPGAESDLEWLDRF
MAKHTKFADRLEPFRADIQRAQRKIGFEEEMVLLPVSHIKEVNRRLSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGL
QFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRVSRQMLQEMGREPT
PEELGERLEMPEDKVRKVLKIAKEPISMETPIGDDDDSHLGDFIEDGTMLLPIDSATGEGLIEATRNVLGGLTAREAKVL
RMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEPLRTFLDE

Sequences:

>Translated_614_residues
MAGNTQQSRLKELIAQGKEQGFLTYAEVNDHLPEDIADPDQVEDIIAMINDMGISVVEEAPDEDTLMMSDQSADESAAEE
AVAALAAVESDVGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEEGTREVMSSLAYLPGAVDSILSAYDATQDEEAPGR
LSDLFSGFIDPDEGIPGVAEAEVPEPEPEPSAADGDVSEDDDEAEEEETGGGPDPEEARARFEQIREQNERAKAAIEEHG
LGSKEASAEQARLAELFSPIKLVPKHFERLVGQVRISVEQIRGQEKAIMQIFVKQAKVPRKTFIGSFPGAESDLEWLDRF
MAKHTKFADRLEPFRADIQRAQRKIGFEEEMVLLPVSHIKEVNRRLSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGL
QFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRVSRQMLQEMGREPT
PEELGERLEMPEDKVRKVLKIAKEPISMETPIGDDDDSHLGDFIEDGTMLLPIDSATGEGLIEATRNVLGGLTAREAKVL
RMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEPLRTFLDE
>Mature_613_residues
AGNTQQSRLKELIAQGKEQGFLTYAEVNDHLPEDIADPDQVEDIIAMINDMGISVVEEAPDEDTLMMSDQSADESAAEEA
VAALAAVESDVGRTTDPVRMYMREMGTVELLTREGEIEIAKRIEEGTREVMSSLAYLPGAVDSILSAYDATQDEEAPGRL
SDLFSGFIDPDEGIPGVAEAEVPEPEPEPSAADGDVSEDDDEAEEEETGGGPDPEEARARFEQIREQNERAKAAIEEHGL
GSKEASAEQARLAELFSPIKLVPKHFERLVGQVRISVEQIRGQEKAIMQIFVKQAKVPRKTFIGSFPGAESDLEWLDRFM
AKHTKFADRLEPFRADIQRAQRKIGFEEEMVLLPVSHIKEVNRRLSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQ
FLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRVSRQMLQEMGREPTP
EELGERLEMPEDKVRKVLKIAKEPISMETPIGDDDDSHLGDFIEDGTMLLPIDSATGEGLIEATRNVLGGLTAREAKVLR
MRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEPLRTFLDE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=610, Percent_Identity=65.0819672131148, Blast_Score=765, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=245, Percent_Identity=42.0408163265306, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=28.5123966942149, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 68999; Mature: 68868

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGNTQQSRLKELIAQGKEQGFLTYAEVNDHLPEDIADPDQVEDIIAMINDMGISVVEEA
CCCCCHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCC
PDEDTLMMSDQSADESAAEEAVAALAAVESDVGRTTDPVRMYMREMGTVELLTREGEIEI
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCHHHH
AKRIEEGTREVMSSLAYLPGAVDSILSAYDATQDEEAPGRLSDLFSGFIDPDEGIPGVAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCC
AEVPEPEPEPSAADGDVSEDDDEAEEEETGGGPDPEEARARFEQIREQNERAKAAIEEHG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LGSKEASAEQARLAELFSPIKLVPKHFERLVGQVRISVEQIRGQEKAIMQIFVKQAKVPR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
KTFIGSFPGAESDLEWLDRFMAKHTKFADRLEPFRADIQRAQRKIGFEEEMVLLPVSHIK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHH
EVNRRLSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKF
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHH
EYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRVSRQMLQEMGREPT
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PEELGERLEMPEDKVRKVLKIAKEPISMETPIGDDDDSHLGDFIEDGTMLLPIDSATGEG
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCH
LIEATRNVLGGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLR
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPSRSEPLRTFLDE
CCCCCCHHHHHHCC
>Mature Secondary Structure 
AGNTQQSRLKELIAQGKEQGFLTYAEVNDHLPEDIADPDQVEDIIAMINDMGISVVEEA
CCCCHHHHHHHHHHCCCCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHHCCCHHHHCC
PDEDTLMMSDQSADESAAEEAVAALAAVESDVGRTTDPVRMYMREMGTVELLTREGEIEI
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHCCCHHHH
AKRIEEGTREVMSSLAYLPGAVDSILSAYDATQDEEAPGRLSDLFSGFIDPDEGIPGVAE
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCC
AEVPEPEPEPSAADGDVSEDDDEAEEEETGGGPDPEEARARFEQIREQNERAKAAIEEHG
CCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LGSKEASAEQARLAELFSPIKLVPKHFERLVGQVRISVEQIRGQEKAIMQIFVKQAKVPR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCH
KTFIGSFPGAESDLEWLDRFMAKHTKFADRLEPFRADIQRAQRKIGFEEEMVLLPVSHIK
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHH
EVNRRLSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKF
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHH
EYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRVSRQMLQEMGREPT
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PEELGERLEMPEDKVRKVLKIAKEPISMETPIGDDDDSHLGDFIEDGTMLLPIDSATGEG
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCH
LIEATRNVLGGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLR
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HPSRSEPLRTFLDE
CCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1902749; 10984043 [H]