Click here to switch to the map view.

The map label for this gene is dnaG [H]

Identifier: 92113103

GI number: 92113103

Start: 1091219

End: 1093060

Strand: Direct

Name: dnaG [H]

Synonym: Csal_0975

Alternate gene names: 92113103

Gene position: 1091219-1093060 (Clockwise)

Preceding gene: 92113102

Following gene: 92113104

Centisome position: 29.52

GC content: 65.58

Gene sequence:

>1842_bases
ATGGCTGGACAGATCCCCCAACGTTTCATCGATGATTTGCTGGCTCGTACCGATGTGGTCGAGATCGTCGGCGAGCGCGT
TCAGCTCAAGAAGGCCGGTCGCAACTACAGCGGTCTATGCCCCTTCCACCAGGAAAAGTCGCCCTCGTTCACCGTCAGCG
CCGAAAAACAGTTCTACCACTGCTTCGGATGTGGCGTGCACGGCAATGCGCTACGCTTTCTGATGGAATTCGATCATTTG
CGCTTTCCGGAGGCGGTCGAGCAGCTCGCCTCGCGGCTGGGGCTGGACGTGCCGCGCGAGGGGGCTGACGACCCTCAGGC
TCAGCAGCGCGAACGGCGGCGCGAGGAGGCGGTTAACCTGCTCGAACTGTCGGCGAGCTTTTTCCGCGAGCGCCTGGCGA
TGTCGGAAGGTGGCGATGCACGCGATTACCTCGAGCGTCGCGGGCTTTCCGCCGATATCGTGCGTGAGTTCGGGATTGGT
TTCGCCCCGGACAAGTGGGAAGCGCTCAAGCGGCACCTGACCGAGCGCGGCATCAGTGATGCGGTGCAGATCGAATACGG
CTTGTTGGTGCACCGCGAGGATTCCGGGCGCAGCTACGACCGTTTTCGCAACCGGGTGATGTTTCCGATCCGCGACTGGA
AAGGGCGCACCATTGCGTTCGGCGGTCGCGTGCTCGGGGACGCCAAACCCAAGTACCTGAACTCGCCCGAGACTCCGGTC
TTCCACAAGGGGCGCGAGCTGTACGGGCTTTACGAGGCGCGTCAGGCCAATGCGCGCCTGGAGCGGATGCTGGTCGTCGA
AGGCTACATGGATGTCGTGGCCCTGGCCCAGTTCGGGATCCGCAATGCCTGCGCCACGCTGGGCACTTCGCTCAGCGAGG
ACCATCTGCAACGCCTGTTTCGGCTGGTCGGCGAAGTGGTGTTCTGCTTCGACGGCGATCGCGCCGGGCGACAGGCGGCG
CGCCGAGCGCTGGAGACGGTGTTGCCGTCGATGATCGATGGGCGCCAGGTGCGGTTTCTGTTTCTGCCGGAGGGCGATGA
TCCGGATAGCCTGGTGCGTCGCGAGGGCGCGGAAGCGTTCGAGAATCGCGTGCTGTGCGCCGCGCCGCTTTCGGAGTTTC
TTTTCGACCAGGCCGCGGAAGGGCGCGATCTCCAGCGGATCGAGGACCGGGAGCGCTTTGCCACGGCGGTGTTGCGTGCT
CTGGAGCGCTTGCCCGAGGGCGTGTTGAAATCCTTGCTGCTGCGCGAGCTTTCGCAGCGTACGGGCGTTGAGCAGGCCAA
TTTTCAGGCCTTGCTGGCGCGTGCCGACACGTCACAGGCGCCGCATGACGAGGGTGCCGATGAGGCCTCGAGCGCTCCTG
AGGACGCAGGCGAGACGCCCGCGAGGAAGGCGGGAGCGCGTGATGGCGCCTTGGGGCTCGTCGGTCGTCTGCTGCACCTG
CTGGTGCATGAGCCCGCTCTGGTGAGTCGCTTGCCCGAGTCGCTCGACTGGTGTCCCGACGGTGTGGGTGACGGGGCGCT
ATGTCATGAGGTAGTGCGTCTGATCCGCGCCGGCGGTTATCGCAACGCGCAGGTCCTGCTGGCGCATTTCCATGGCACGC
CCGAGGGGGAGCGGCTGGCCTGGCTGGCGCGTCGTGAACTCTTGATGCCCCGCGAGGCACGAGGGGCCGAGGTGGAGGGC
CTGGTCGACGGGTTGCGTCGACGCCACTCGCAGCGCTCGCCACAGGAGGAGTATGACGCCCTGCTGGCACGGACGCAGGC
GGGGGAGTGTCTGTCCAGGGAAGAGCGTCAGCGGCTGAACGAGTTGCTGATGGAGCTCAAGGGAGGCGCTCGGCCGCCAT
GA

Upstream 100 bases:

>100_bases
CATCGGGTGGCCGCTTGTTTTTCCCGGCCCACCGTTTCCGCGATGCCTGGCTCCGCGATTCCCGCGATACAGACAACGTT
TCGTCGAGCGCCTCCCCGCC

Downstream 100 bases:

>100_bases
AACGGCTTGAACGGATGCCTGGCGCGACGGGTTGAATTGCCGCAAAACGTCGCCATTTACTAGGCAATATCGAAAAGGTA
GCCGAACACACTAGCACACC

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 613; Mature: 612

Protein sequence:

>613_residues
MAGQIPQRFIDDLLARTDVVEIVGERVQLKKAGRNYSGLCPFHQEKSPSFTVSAEKQFYHCFGCGVHGNALRFLMEFDHL
RFPEAVEQLASRLGLDVPREGADDPQAQQRERRREEAVNLLELSASFFRERLAMSEGGDARDYLERRGLSADIVREFGIG
FAPDKWEALKRHLTERGISDAVQIEYGLLVHREDSGRSYDRFRNRVMFPIRDWKGRTIAFGGRVLGDAKPKYLNSPETPV
FHKGRELYGLYEARQANARLERMLVVEGYMDVVALAQFGIRNACATLGTSLSEDHLQRLFRLVGEVVFCFDGDRAGRQAA
RRALETVLPSMIDGRQVRFLFLPEGDDPDSLVRREGAEAFENRVLCAAPLSEFLFDQAAEGRDLQRIEDRERFATAVLRA
LERLPEGVLKSLLLRELSQRTGVEQANFQALLARADTSQAPHDEGADEASSAPEDAGETPARKAGARDGALGLVGRLLHL
LVHEPALVSRLPESLDWCPDGVGDGALCHEVVRLIRAGGYRNAQVLLAHFHGTPEGERLAWLARRELLMPREARGAEVEG
LVDGLRRRHSQRSPQEEYDALLARTQAGECLSREERQRLNELLMELKGGARPP

Sequences:

>Translated_613_residues
MAGQIPQRFIDDLLARTDVVEIVGERVQLKKAGRNYSGLCPFHQEKSPSFTVSAEKQFYHCFGCGVHGNALRFLMEFDHL
RFPEAVEQLASRLGLDVPREGADDPQAQQRERRREEAVNLLELSASFFRERLAMSEGGDARDYLERRGLSADIVREFGIG
FAPDKWEALKRHLTERGISDAVQIEYGLLVHREDSGRSYDRFRNRVMFPIRDWKGRTIAFGGRVLGDAKPKYLNSPETPV
FHKGRELYGLYEARQANARLERMLVVEGYMDVVALAQFGIRNACATLGTSLSEDHLQRLFRLVGEVVFCFDGDRAGRQAA
RRALETVLPSMIDGRQVRFLFLPEGDDPDSLVRREGAEAFENRVLCAAPLSEFLFDQAAEGRDLQRIEDRERFATAVLRA
LERLPEGVLKSLLLRELSQRTGVEQANFQALLARADTSQAPHDEGADEASSAPEDAGETPARKAGARDGALGLVGRLLHL
LVHEPALVSRLPESLDWCPDGVGDGALCHEVVRLIRAGGYRNAQVLLAHFHGTPEGERLAWLARRELLMPREARGAEVEG
LVDGLRRRHSQRSPQEEYDALLARTQAGECLSREERQRLNELLMELKGGARPP
>Mature_612_residues
AGQIPQRFIDDLLARTDVVEIVGERVQLKKAGRNYSGLCPFHQEKSPSFTVSAEKQFYHCFGCGVHGNALRFLMEFDHLR
FPEAVEQLASRLGLDVPREGADDPQAQQRERRREEAVNLLELSASFFRERLAMSEGGDARDYLERRGLSADIVREFGIGF
APDKWEALKRHLTERGISDAVQIEYGLLVHREDSGRSYDRFRNRVMFPIRDWKGRTIAFGGRVLGDAKPKYLNSPETPVF
HKGRELYGLYEARQANARLERMLVVEGYMDVVALAQFGIRNACATLGTSLSEDHLQRLFRLVGEVVFCFDGDRAGRQAAR
RALETVLPSMIDGRQVRFLFLPEGDDPDSLVRREGAEAFENRVLCAAPLSEFLFDQAAEGRDLQRIEDRERFATAVLRAL
ERLPEGVLKSLLLRELSQRTGVEQANFQALLARADTSQAPHDEGADEASSAPEDAGETPARKAGARDGALGLVGRLLHLL
VHEPALVSRLPESLDWCPDGVGDGALCHEVVRLIRAGGYRNAQVLLAHFHGTPEGERLAWLARRELLMPREARGAEVEGL
VDGLRRRHSQRSPQEEYDALLARTQAGECLSREERQRLNELLMELKGGARPP

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=544, Percent_Identity=47.0588235294118, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68790; Mature: 68659

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGQIPQRFIDDLLARTDVVEIVGERVQLKKAGRNYSGLCPFHQEKSPSFTVSAEKQFYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHEEH
CFGCGVHGNALRFLMEFDHLRFPEAVEQLASRLGLDVPREGADDPQAQQRERRREEAVNL
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LELSASFFRERLAMSEGGDARDYLERRGLSADIVREFGIGFAPDKWEALKRHLTERGISD
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCH
AVQIEYGLLVHREDSGRSYDRFRNRVMFPIRDWKGRTIAFGGRVLGDAKPKYLNSPETPV
HHEEEEEEEEEECCCCCHHHHHHCCEECEEECCCCCEEEECCEEECCCCCCCCCCCCCCH
FHKGRELYGLYEARQANARLERMLVVEGYMDVVALAQFGIRNACATLGTSLSEDHLQRLF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RLVGEVVFCFDGDRAGRQAARRALETVLPSMIDGRQVRFLFLPEGDDPDSLVRREGAEAF
HHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHH
ENRVLCAAPLSEFLFDQAAEGRDLQRIEDRERFATAVLRALERLPEGVLKSLLLRELSQR
HCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVEQANFQALLARADTSQAPHDEGADEASSAPEDAGETPARKAGARDGALGLVGRLLHL
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHH
LVHEPALVSRLPESLDWCPDGVGDGALCHEVVRLIRAGGYRNAQVLLAHFHGTPEGERLA
HHCCCHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCHHHH
WLARRELLMPREARGAEVEGLVDGLRRRHSQRSPQEEYDALLARTQAGECLSREERQRLN
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLMELKGGARPP
HHHHHHCCCCCCC
>Mature Secondary Structure 
AGQIPQRFIDDLLARTDVVEIVGERVQLKKAGRNYSGLCPFHQEKSPSFTVSAEKQFYH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEHHHHEEH
CFGCGVHGNALRFLMEFDHLRFPEAVEQLASRLGLDVPREGADDPQAQQRERRREEAVNL
HHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
LELSASFFRERLAMSEGGDARDYLERRGLSADIVREFGIGFAPDKWEALKRHLTERGISD
HHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHCCCCH
AVQIEYGLLVHREDSGRSYDRFRNRVMFPIRDWKGRTIAFGGRVLGDAKPKYLNSPETPV
HHEEEEEEEEEECCCCCHHHHHHCCEECEEECCCCCEEEECCEEECCCCCCCCCCCCCCH
FHKGRELYGLYEARQANARLERMLVVEGYMDVVALAQFGIRNACATLGTSLSEDHLQRLF
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
RLVGEVVFCFDGDRAGRQAARRALETVLPSMIDGRQVRFLFLPEGDDPDSLVRREGAEAF
HHHHHEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHCCHHHH
ENRVLCAAPLSEFLFDQAAEGRDLQRIEDRERFATAVLRALERLPEGVLKSLLLRELSQR
HCCEEEHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGVEQANFQALLARADTSQAPHDEGADEASSAPEDAGETPARKAGARDGALGLVGRLLHL
CCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHH
LVHEPALVSRLPESLDWCPDGVGDGALCHEVVRLIRAGGYRNAQVLLAHFHGTPEGERLA
HHCCCHHHHHCHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEEEECCCCCCCHHHH
WLARRELLMPREARGAEVEGLVDGLRRRHSQRSPQEEYDALLARTQAGECLSREERQRLN
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ELLMELKGGARPP
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]