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The map label for this gene is kefB [H]

Identifier: 92113072

GI number: 92113072

Start: 1058672

End: 1060504

Strand: Direct

Name: kefB [H]

Synonym: Csal_0944

Alternate gene names: 92113072

Gene position: 1058672-1060504 (Clockwise)

Preceding gene: 92113071

Following gene: 92113073

Centisome position: 28.64

GC content: 64.76

Gene sequence:

>1833_bases
ATGACGTTCTTTGCCGAGGCGGCGGTGTTGCTGGGAGCGGCGATCATCGCCGTGCCATTGTTTCAACGCCTGGGACTGGG
CTCGATCCTGGGCTATCTCGCCGTGGGGCTGCTGCTGGGGCCCTCATTGATCGGCTTCATCAGCGAACCGGAGGAAGTAC
TGCATTTCTCCGAATTCGGCGTCGTGATGTTGCTGTTCATCATCGGGCTGGAGCTGGAACCGTCGCGCCTGTGGGGGATG
CGGAAACGCCTGTTCGGACTGGGCCTGCTTCAATTGTTCGGCTGCGGCGTGCTGCTGACGCCGATCGGCCTGGCGTTGGG
CCTGAGGTGGGAGCTGGCGGTACTGCTGGGATTGATTCTGGCCTTGTCCTCCACGGCCTTTGCCCTGCAGGTGCTCAACG
AGCAGCGTCGCCTGGCCACACCGCACGGGCAGACGGCGTTCGCGATCCTGCTCTTCCAGGATCTGGCGGTGATTCCCTTG
CTGGCCTTGCTGCCGCTGTTCGCGGGTGGGGTGAGCGCACAGGGCGGCGACATGTGGCTCGACATCGGCAAGGGCGTGGG
CATGGTGCTGGCCGCGATCGTGTTCGGTCGTCTGGTGTTTCCCCGCGTGCTGGCGCTCATCGCGCGTAGCGGCGTGCATG
AGGTCTTCACCGCCGCGGCCCTGTTCCTGGTGCTGACCCTGGCCTTGGTGATGGATTGGGCGGGCCTGTCCATGGCGCTG
GGCGCCTTCCTGGGAGGCGTCATGCTGGCGGACACGATTTATCGCCATGAACTGGAAGCCAACATCGAGCCGTTCAAGGG
CATTCTGCTGGGCCTGTTCTTCATGGCCGTGGGCATGTCGGTCAACCTGGCGCTGGTCTTCGACAATGTCTTCTGGGTGC
TGGGAATCACCCTGGTGCTGTTGGCGGCCAAGCTCGTCATCATCGCCCTGGTGGGCATGGCGGCACGCTTGCCGCGACAA
GAGATTCCGCGCCTGGCAACGGTGATCGCGCAAGGAGGGGAGTTCGATTTCGTGCTGCTCAGCGCGGCGGTCAGCGCCGG
CGTGTTCGATCAGCGCATCGCCAGCCTGTGCATCGCCGCGGTGACCTTGAGCATGGCCTTGACGCCGTTCTTGTATCGTG
GCGGCAACCACCTCGCCGACCGCATGCGGGAGACCCGCCCTTACGACAACGATTTCGGGGACGAGACGCCTCCCGTGCTG
ATCGCGGGGCTGGGGCGTTTCGGCCAGATGGTGGCCCGTGTGCTCAAGCTGCAGGACATCAGCTTTACCGCGCTCGACCC
CAACATCACGCAAGTGGAATTCATACGGCGCTTCGGCTCGCGTATCTACTACGCCGACGCCACGCGGCTGGATGTGCTGC
GCTCGGCGGGACTGGAACACGCCCGCGTGCTGGTGATCGCCGTCGACGACGAGGAGGCGGCGCTGACCATCGCCCGGCAG
GTGCGTACTCACTATCGCCAGGTCAAGGTCTTCGCGCGTGCGCGCAACCGCCACCATGCCTGGCAGCTCATGGAAGCCGG
CGTCGATGCCGTGGTGCGCGAGACCTTTGCCTCGAGCATGGAGATGAGCGTCGAGGTGCTCAAGGGACTCGGCTACCCGG
GATCCAATGCCCTGGATGCCGTGCGCACGTTCCGCGACTTCGACAATCAGCTGTTGGCCAGGACCTTCATGCATCGGCAT
GATTCGGAAGCGTTGATGGCGACGGAGCGCGCGGCCATGGCGGAGTTCCAGACACTGTTCCAGCAGGAACTGGGCCAAGG
CCGAGGGCAGGAGGAGGCCGCCGAGCCCTCGGACGTGTCGGTCGAAACGTCGACCGAGGACGTTCGGCGGTAG

Upstream 100 bases:

>100_bases
AGTGAGCCCGCGACGTATTCGGCGTGCGTGGCATGGCCTACCTCAATAGACCCTGTCTCATGGCACCGGCCTCGAGACGT
GACGTAAGGAAAACAGGGAC

Downstream 100 bases:

>100_bases
ATGGCGGTCGCGTCGCCGCGCCGGTCTTTCACAGACGAAAACGAGGAGAGCCGACATGGAGCAGTCACTGAGCACGTTGC
TGGCGTCTTTGGAGATCGTC

Product: potassium efflux system protein

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: K(+)/H(+) antiporter; NEM-activatable K(+)/H(+) antiporter [H]

Number of amino acids: Translated: 610; Mature: 609

Protein sequence:

>610_residues
MTFFAEAAVLLGAAIIAVPLFQRLGLGSILGYLAVGLLLGPSLIGFISEPEEVLHFSEFGVVMLLFIIGLELEPSRLWGM
RKRLFGLGLLQLFGCGVLLTPIGLALGLRWELAVLLGLILALSSTAFALQVLNEQRRLATPHGQTAFAILLFQDLAVIPL
LALLPLFAGGVSAQGGDMWLDIGKGVGMVLAAIVFGRLVFPRVLALIARSGVHEVFTAAALFLVLTLALVMDWAGLSMAL
GAFLGGVMLADTIYRHELEANIEPFKGILLGLFFMAVGMSVNLALVFDNVFWVLGITLVLLAAKLVIIALVGMAARLPRQ
EIPRLATVIAQGGEFDFVLLSAAVSAGVFDQRIASLCIAAVTLSMALTPFLYRGGNHLADRMRETRPYDNDFGDETPPVL
IAGLGRFGQMVARVLKLQDISFTALDPNITQVEFIRRFGSRIYYADATRLDVLRSAGLEHARVLVIAVDDEEAALTIARQ
VRTHYRQVKVFARARNRHHAWQLMEAGVDAVVRETFASSMEMSVEVLKGLGYPGSNALDAVRTFRDFDNQLLARTFMHRH
DSEALMATERAAMAEFQTLFQQELGQGRGQEEAAEPSDVSVETSTEDVRR

Sequences:

>Translated_610_residues
MTFFAEAAVLLGAAIIAVPLFQRLGLGSILGYLAVGLLLGPSLIGFISEPEEVLHFSEFGVVMLLFIIGLELEPSRLWGM
RKRLFGLGLLQLFGCGVLLTPIGLALGLRWELAVLLGLILALSSTAFALQVLNEQRRLATPHGQTAFAILLFQDLAVIPL
LALLPLFAGGVSAQGGDMWLDIGKGVGMVLAAIVFGRLVFPRVLALIARSGVHEVFTAAALFLVLTLALVMDWAGLSMAL
GAFLGGVMLADTIYRHELEANIEPFKGILLGLFFMAVGMSVNLALVFDNVFWVLGITLVLLAAKLVIIALVGMAARLPRQ
EIPRLATVIAQGGEFDFVLLSAAVSAGVFDQRIASLCIAAVTLSMALTPFLYRGGNHLADRMRETRPYDNDFGDETPPVL
IAGLGRFGQMVARVLKLQDISFTALDPNITQVEFIRRFGSRIYYADATRLDVLRSAGLEHARVLVIAVDDEEAALTIARQ
VRTHYRQVKVFARARNRHHAWQLMEAGVDAVVRETFASSMEMSVEVLKGLGYPGSNALDAVRTFRDFDNQLLARTFMHRH
DSEALMATERAAMAEFQTLFQQELGQGRGQEEAAEPSDVSVETSTEDVRR
>Mature_609_residues
TFFAEAAVLLGAAIIAVPLFQRLGLGSILGYLAVGLLLGPSLIGFISEPEEVLHFSEFGVVMLLFIIGLELEPSRLWGMR
KRLFGLGLLQLFGCGVLLTPIGLALGLRWELAVLLGLILALSSTAFALQVLNEQRRLATPHGQTAFAILLFQDLAVIPLL
ALLPLFAGGVSAQGGDMWLDIGKGVGMVLAAIVFGRLVFPRVLALIARSGVHEVFTAAALFLVLTLALVMDWAGLSMALG
AFLGGVMLADTIYRHELEANIEPFKGILLGLFFMAVGMSVNLALVFDNVFWVLGITLVLLAAKLVIIALVGMAARLPRQE
IPRLATVIAQGGEFDFVLLSAAVSAGVFDQRIASLCIAAVTLSMALTPFLYRGGNHLADRMRETRPYDNDFGDETPPVLI
AGLGRFGQMVARVLKLQDISFTALDPNITQVEFIRRFGSRIYYADATRLDVLRSAGLEHARVLVIAVDDEEAALTIARQV
RTHYRQVKVFARARNRHHAWQLMEAGVDAVVRETFASSMEMSVEVLKGLGYPGSNALDAVRTFRDFDNQLLARTFMHRHD
SEALMATERAAMAEFQTLFQQELGQGRGQEEAAEPSDVSVETSTEDVRR

Specific function: Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RCK N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI157388921, Length=382, Percent_Identity=29.0575916230367, Blast_Score=92, Evalue=2e-18,
Organism=Escherichia coli, GI1786232, Length=610, Percent_Identity=40.327868852459, Blast_Score=430, Evalue=1e-121,
Organism=Escherichia coli, GI1789749, Length=594, Percent_Identity=42.2558922558923, Blast_Score=417, Evalue=1e-117,
Organism=Escherichia coli, GI1786685, Length=531, Percent_Identity=26.5536723163842, Blast_Score=142, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153
- InterPro:   IPR020884
- InterPro:   IPR004771
- InterPro:   IPR016040
- InterPro:   IPR003148 [H]

Pfam domain/function: PF00999 Na_H_Exchanger; PF02254 TrkA_N [H]

EC number: NA

Molecular weight: Translated: 66376; Mature: 66245

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFFAEAAVLLGAAIIAVPLFQRLGLGSILGYLAVGLLLGPSLIGFISEPEEVLHFSEFG
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VVMLLFIIGLELEPSRLWGMRKRLFGLGLLQLFGCGVLLTPIGLALGLRWELAVLLGLIL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALSSTAFALQVLNEQRRLATPHGQTAFAILLFQDLAVIPLLALLPLFAGGVSAQGGDMWL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
DIGKGVGMVLAAIVFGRLVFPRVLALIARSGVHEVFTAAALFLVLTLALVMDWAGLSMAL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAFLGGVMLADTIYRHELEANIEPFKGILLGLFFMAVGMSVNLALVFDNVFWVLGITLVL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
LAAKLVIIALVGMAARLPRQEIPRLATVIAQGGEFDFVLLSAAVSAGVFDQRIASLCIAA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VTLSMALTPFLYRGGNHLADRMRETRPYDNDFGDETPPVLIAGLGRFGQMVARVLKLQDI
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SFTALDPNITQVEFIRRFGSRIYYADATRLDVLRSAGLEHARVLVIAVDDEEAALTIARQ
EEEECCCCCHHHHHHHHHCCEEEEECHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHH
VRTHYRQVKVFARARNRHHAWQLMEAGVDAVVRETFASSMEMSVEVLKGLGYPGSNALDA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
VRTFRDFDNQLLARTFMHRHDSEALMATERAAMAEFQTLFQQELGQGRGQEEAAEPSDVS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
VETSTEDVRR
EECCHHHHCC
>Mature Secondary Structure 
TFFAEAAVLLGAAIIAVPLFQRLGLGSILGYLAVGLLLGPSLIGFISEPEEVLHFSEFG
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VVMLLFIIGLELEPSRLWGMRKRLFGLGLLQLFGCGVLLTPIGLALGLRWELAVLLGLIL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALSSTAFALQVLNEQRRLATPHGQTAFAILLFQDLAVIPLLALLPLFAGGVSAQGGDMWL
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEE
DIGKGVGMVLAAIVFGRLVFPRVLALIARSGVHEVFTAAALFLVLTLALVMDWAGLSMAL
ECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GAFLGGVMLADTIYRHELEANIEPFKGILLGLFFMAVGMSVNLALVFDNVFWVLGITLVL
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHH
LAAKLVIIALVGMAARLPRQEIPRLATVIAQGGEFDFVLLSAAVSAGVFDQRIASLCIAA
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VTLSMALTPFLYRGGNHLADRMRETRPYDNDFGDETPPVLIAGLGRFGQMVARVLKLQDI
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
SFTALDPNITQVEFIRRFGSRIYYADATRLDVLRSAGLEHARVLVIAVDDEEAALTIARQ
EEEECCCCCHHHHHHHHHCCEEEEECHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHH
VRTHYRQVKVFARARNRHHAWQLMEAGVDAVVRETFASSMEMSVEVLKGLGYPGSNALDA
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
VRTFRDFDNQLLARTFMHRHDSEALMATERAAMAEFQTLFQQELGQGRGQEEAAEPSDVS
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
VETSTEDVRR
EECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA