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The map label for this gene is cca [H]

Identifier: 92113052

GI number: 92113052

Start: 1037355

End: 1038509

Strand: Direct

Name: cca [H]

Synonym: Csal_0924

Alternate gene names: 92113052

Gene position: 1037355-1038509 (Clockwise)

Preceding gene: 92113051

Following gene: 92113054

Centisome position: 28.06

GC content: 68.83

Gene sequence:

>1155_bases
GTGTCGAGAGCTGAGACCGTGAAGCCCGATGATGCCCTCGAAGGACTCGACGTCTATCGCGTGGGCGGCGCGGTGCGTGA
CGCGCGCCTGGGGTGGCCGGTCACCGACGAGGACTGGGTCGTGGTCGGCGCCACGCCGGAGGCGCTCACGGCGCGGGGGT
TCGTCCCCGTCGGGCGTGACTTCCCGGTGTTCCTGCATCCCGTCACCCATGAGGAATATGCCCTGGCGCGCACCGAGCGC
AAGCAGGGTCATGGCTATACCGGTTTCGTGGTCCATGCCAGCCCCGAGGTGACGCTGGAAGAGGATCTGGCGCGCCGCGA
CCTGACCATCAACGCCATGGCCGAGGATCGCGACGGCCGGCTGGTCGATCCGTTCGATGGCCGGCAGGACCTCGATGCCC
GCGTGCTGCGCCATGTCTCCGAGGCCTTCGTCGAAGACCCCTTGCGTGTGTTGCGCACGGCGCGTTTCTGGGCACGCTAC
CGTGAGTTGGGGTTCGTCATTGCCGACGATACCAAGCGTCTGATGCGGGGGCTGTGCGACAACGGTGAAATCGCTCACCT
GGTCGCCGAGCGCGTGTGGCAGGAGACCGTCAAGGCCCTGGGCGAGCCTCACCCCATGGCCTACTTCCAGGCGCTGCACG
AGTGCGGTGCTCTTGCCGTGTGGATGCCGGAACTGGATGCCTCCGACATGCTCGCGCGTGGCGAAGCGGCCATGGCGAGA
CTGCCCGCCGGCGGCGAGCCTTCGCAGTGGCGCTGGGCGCGCCTGCTCGAAGCACTGGAGGACGAGGCGCGCGAGGCCTT
GTGCGAACGCCTGCGCGTGCCCAATGCATGGCGGGATCTGGCCCGCCAGGTGGCACGTACCCGCGCGCTGCAGCGGCAGC
ACGGCGATGGCGGGTTGGAGGGCTCCGACGTGATGGCCTGGTTCGATGCCATCGATGCCTGGCGGCGGTCCGAGCGTGTC
GCGCCGCTGCAGCGTTACCTGGCGATGGAAACGCCGGCGCTGGCCGCATGCGTCGATGAGGCCTGGCAGGCCGCGAGTGC
GATCCAGCCGCAGGCGTTGCTCGAGGAGGGCTATCGCGGCAAGGCACTGGGCGACGCGCTGGCACAGCGGCGTCAGGCCC
GTATCGATGAGGTGGTCGGGCAGTGTAACGTGTAA

Upstream 100 bases:

>100_bases
TCCAGTCAGCAGGCGGCCAATTGGCTGGCGGGGCAGGGGTTCAGCGACGTCTATAGCCTGGATGGCGGCTTCACCGACTG
GGCCGCGCGCCATCCCGATC

Downstream 100 bases:

>100_bases
GCCCTGGCCGTGATCGCGCTGCGTGCCACGGAAAGAGACACACCGCGATATGCAAACGCAGAAGGGGCCATCGAGGCCCC
TTCTGCGTTCTGCGTTTCAT

Product: polynucleotide adenylyltransferase region

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSRAETVKPDDALEGLDVYRVGGAVRDARLGWPVTDEDWVVVGATPEALTARGFVPVGRDFPVFLHPVTHEEYALARTER
KQGHGYTGFVVHASPEVTLEEDLARRDLTINAMAEDRDGRLVDPFDGRQDLDARVLRHVSEAFVEDPLRVLRTARFWARY
RELGFVIADDTKRLMRGLCDNGEIAHLVAERVWQETVKALGEPHPMAYFQALHECGALAVWMPELDASDMLARGEAAMAR
LPAGGEPSQWRWARLLEALEDEAREALCERLRVPNAWRDLARQVARTRALQRQHGDGGLEGSDVMAWFDAIDAWRRSERV
APLQRYLAMETPALAACVDEAWQAASAIQPQALLEEGYRGKALGDALAQRRQARIDEVVGQCNV

Sequences:

>Translated_384_residues
MSRAETVKPDDALEGLDVYRVGGAVRDARLGWPVTDEDWVVVGATPEALTARGFVPVGRDFPVFLHPVTHEEYALARTER
KQGHGYTGFVVHASPEVTLEEDLARRDLTINAMAEDRDGRLVDPFDGRQDLDARVLRHVSEAFVEDPLRVLRTARFWARY
RELGFVIADDTKRLMRGLCDNGEIAHLVAERVWQETVKALGEPHPMAYFQALHECGALAVWMPELDASDMLARGEAAMAR
LPAGGEPSQWRWARLLEALEDEAREALCERLRVPNAWRDLARQVARTRALQRQHGDGGLEGSDVMAWFDAIDAWRRSERV
APLQRYLAMETPALAACVDEAWQAASAIQPQALLEEGYRGKALGDALAQRRQARIDEVVGQCNV
>Mature_383_residues
SRAETVKPDDALEGLDVYRVGGAVRDARLGWPVTDEDWVVVGATPEALTARGFVPVGRDFPVFLHPVTHEEYALARTERK
QGHGYTGFVVHASPEVTLEEDLARRDLTINAMAEDRDGRLVDPFDGRQDLDARVLRHVSEAFVEDPLRVLRTARFWARYR
ELGFVIADDTKRLMRGLCDNGEIAHLVAERVWQETVKALGEPHPMAYFQALHECGALAVWMPELDASDMLARGEAAMARL
PAGGEPSQWRWARLLEALEDEAREALCERLRVPNAWRDLARQVARTRALQRQHGDGGLEGSDVMAWFDAIDAWRRSERVA
PLQRYLAMETPALAACVDEAWQAASAIQPQALLEEGYRGKALGDALAQRRQARIDEVVGQCNV

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789436, Length=403, Percent_Identity=44.9131513647643, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 42991; Mature: 42860

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAETVKPDDALEGLDVYRVGGAVRDARLGWPVTDEDWVVVGATPEALTARGFVPVGRD
CCCCCCCCCCHHHCCCHHHHHCCCHHHCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCC
FPVFLHPVTHEEYALARTERKQGHGYTGFVVHASPEVTLEEDLARRDLTINAMAEDRDGR
CCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCCC
LVDPFDGRQDLDARVLRHVSEAFVEDPLRVLRTARFWARYRELGFVIADDTKRLMRGLCD
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHCCC
NGEIAHLVAERVWQETVKALGEPHPMAYFQALHECGALAVWMPELDASDMLARGEAAMAR
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCHHHHH
LPAGGEPSQWRWARLLEALEDEAREALCERLRVPNAWRDLARQVARTRALQRQHGDGGLE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GSDVMAWFDAIDAWRRSERVAPLQRYLAMETPALAACVDEAWQAASAIQPQALLEEGYRG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCC
KALGDALAQRRQARIDEVVGQCNV
CHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRAETVKPDDALEGLDVYRVGGAVRDARLGWPVTDEDWVVVGATPEALTARGFVPVGRD
CCCCCCCCCHHHCCCHHHHHCCCHHHCCCCCCCCCCCEEEEECCCHHHHHCCCCCCCCC
FPVFLHPVTHEEYALARTERKQGHGYTGFVVHASPEVTLEEDLARRDLTINAMAEDRDGR
CCEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEECCCCCC
LVDPFDGRQDLDARVLRHVSEAFVEDPLRVLRTARFWARYRELGFVIADDTKRLMRGLCD
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCHHHHHHHCCC
NGEIAHLVAERVWQETVKALGEPHPMAYFQALHECGALAVWMPELDASDMLARGEAAMAR
CCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCHHHHHHHCCHHHHH
LPAGGEPSQWRWARLLEALEDEAREALCERLRVPNAWRDLARQVARTRALQRQHGDGGLE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
GSDVMAWFDAIDAWRRSERVAPLQRYLAMETPALAACVDEAWQAASAIQPQALLEEGYRG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCC
KALGDALAQRRQARIDEVVGQCNV
CHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA