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The map label for this gene is ywiE [H]

Identifier: 92112990

GI number: 92112990

Start: 966228

End: 967340

Strand: Direct

Name: ywiE [H]

Synonym: Csal_0862

Alternate gene names: 92112990

Gene position: 966228-967340 (Clockwise)

Preceding gene: 92112988

Following gene: 92112992

Centisome position: 26.14

GC content: 66.31

Gene sequence:

>1113_bases
ATGCGCGGACGTTGGCAGGGAGGCAACCGGTTCACGTTGCTGCCGGAGGGGAAGCGATTTCTCCCCGAATTGTTTGCCGC
GATCGAATCGGCGCAGCGCACGCTGTATCTCGAGCTGTACCTCATGGAATCCGGCACCCTGGCCACGCGCATGGTCGATG
CCTTGTGCGCGGCGGCGCACCGTGGGGTCACGGTGCGCGTGCTGCTCGACGGCTACGGATCGATGAAACTCTCCCACGCC
GATCGCCAGCGCCTGCGCGATGCCGGGGTGGCGCTGCGCTTCTTCAATCCCCTGAGCTGGCATCGCCTGGGGGGCAATTT
GTCGCGCGATCACCGCAAGCTGCTGGTCGTCGACGACCGGCTGGCCTTCACGGGCGGTTTCGGTGCCGTGGATGCCTTCG
TCGATGCCTGGTATGAAGTGGCCGTGCGCATCGAAGGGCCGGTGGTCGCGGACTGGATCGGCCTGTTCGCGCAGCTCTGG
GAATCGCCCCTGACCCGTGGGCGCGCGGCGCGGTGTCGCTGGCCCGAACCCGCCCCCATCGCGGCGCTGCCGGACGACAT
GCGCGGGCGCATGATCAGCGGGCGGGGCCACCGGTATCAGGCGATTCGCCTATCGTTGCATCAGCGCATCGCCACCGCCG
AACGTCGGCTGTGGTTATGTACGCCGTATTTCATGCCGACGTTCAGCCTGCGCCGTCGGCTGGTGCGGGCGGCGCGGCTG
GGCGTCGATGTGCGTCTGCTGCTGCCGGGCTCGCGTCACGATCATCCCGGCATCCGTTATGCCGGGCAGCGTTTCTATGC
GCGATTGCTGCGTGCGGGCGTGCGCATCTACGAGTTCCAGCCCACCTTCATCCATGCCAAGTTCGTGCTGGCCGACGACT
GGACCTCGATCGGTTCGTGCAATTTCGATCACTGGAGCCTGCAGTGGAATCTGGAGGCCAATCAGGAAGTCGAGGACGTA
CGCTTCGCGCGCGACGTGGCAGCGCTTTTCGAACGCAACTTCGCCGCGAGCGAAGAGATCCATTGGGCGCAATGGCGTCG
CCGTCCCTGGATGCAGCGCTGCCGCGAGTGGGTCTTCGGCACGCTCAACGGGTGGATGACGCGGCTGCGCTGA

Upstream 100 bases:

>100_bases
GAGATCGACTCGCCCAACGGCGCCGCTTTTCAAGGGCATGGCAAACGTGCGTCAATACTGAATCACGCGAGCCGCCGAGA
TCGGTCACCAAGGAGATACC

Downstream 100 bases:

>100_bases
CGATCGCCTAGCGGCGCCCCTTGCCGCCCGCGCCCCCGGCGGGTTTTTTCTTGCGCGGGGTGGGCTTGGGTTTTTCCTCG
GGGACCCGGTAGTGCAGGTA

Product: phospholipase D/transphosphatidylase

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase 1 [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MRGRWQGGNRFTLLPEGKRFLPELFAAIESAQRTLYLELYLMESGTLATRMVDALCAAAHRGVTVRVLLDGYGSMKLSHA
DRQRLRDAGVALRFFNPLSWHRLGGNLSRDHRKLLVVDDRLAFTGGFGAVDAFVDAWYEVAVRIEGPVVADWIGLFAQLW
ESPLTRGRAARCRWPEPAPIAALPDDMRGRMISGRGHRYQAIRLSLHQRIATAERRLWLCTPYFMPTFSLRRRLVRAARL
GVDVRLLLPGSRHDHPGIRYAGQRFYARLLRAGVRIYEFQPTFIHAKFVLADDWTSIGSCNFDHWSLQWNLEANQEVEDV
RFARDVAALFERNFAASEEIHWAQWRRRPWMQRCREWVFGTLNGWMTRLR

Sequences:

>Translated_370_residues
MRGRWQGGNRFTLLPEGKRFLPELFAAIESAQRTLYLELYLMESGTLATRMVDALCAAAHRGVTVRVLLDGYGSMKLSHA
DRQRLRDAGVALRFFNPLSWHRLGGNLSRDHRKLLVVDDRLAFTGGFGAVDAFVDAWYEVAVRIEGPVVADWIGLFAQLW
ESPLTRGRAARCRWPEPAPIAALPDDMRGRMISGRGHRYQAIRLSLHQRIATAERRLWLCTPYFMPTFSLRRRLVRAARL
GVDVRLLLPGSRHDHPGIRYAGQRFYARLLRAGVRIYEFQPTFIHAKFVLADDWTSIGSCNFDHWSLQWNLEANQEVEDV
RFARDVAALFERNFAASEEIHWAQWRRRPWMQRCREWVFGTLNGWMTRLR
>Mature_370_residues
MRGRWQGGNRFTLLPEGKRFLPELFAAIESAQRTLYLELYLMESGTLATRMVDALCAAAHRGVTVRVLLDGYGSMKLSHA
DRQRLRDAGVALRFFNPLSWHRLGGNLSRDHRKLLVVDDRLAFTGGFGAVDAFVDAWYEVAVRIEGPVVADWIGLFAQLW
ESPLTRGRAARCRWPEPAPIAALPDDMRGRMISGRGHRYQAIRLSLHQRIATAERRLWLCTPYFMPTFSLRRRLVRAARL
GVDVRLLLPGSRHDHPGIRYAGQRFYARLLRAGVRIYEFQPTFIHAKFVLADDWTSIGSCNFDHWSLQWNLEANQEVEDV
RFARDVAALFERNFAASEEIHWAQWRRRPWMQRCREWVFGTLNGWMTRLR

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=337, Percent_Identity=33.5311572700297, Blast_Score=151, Evalue=8e-38,
Organism=Escherichia coli, GI1787502, Length=368, Percent_Identity=29.0760869565217, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 42892; Mature: 42892

Theoretical pI: Translated: 11.00; Mature: 11.00

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGRWQGGNRFTLLPEGKRFLPELFAAIESAQRTLYLELYLMESGTLATRMVDALCAAAH
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
RGVTVRVLLDGYGSMKLSHADRQRLRDAGVALRFFNPLSWHRLGGNLSRDHRKLLVVDDR
CCCEEEEEECCCCCEEECHHHHHHHHHHCEEEEECCCCHHHHCCCCCCCCCCEEEEEECC
LAFTGGFGAVDAFVDAWYEVAVRIEGPVVADWIGLFAQLWESPLTRGRAARCRWPEPAPI
EEEECCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AALPDDMRGRMISGRGHRYQAIRLSLHQRIATAERRLWLCTPYFMPTFSLRRRLVRAARL
CCCCCHHCCCEECCCCCEEHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHC
GVDVRLLLPGSRHDHPGIRYAGQRFYARLLRAGVRIYEFQPTFIHAKFVLADDWTSIGSC
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEECCCCCCCCC
NFDHWSLQWNLEANQEVEDVRFARDVAALFERNFAASEEIHWAQWRRRPWMQRCREWVFG
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHH
TLNGWMTRLR
HHHHHHHHCC
>Mature Secondary Structure
MRGRWQGGNRFTLLPEGKRFLPELFAAIESAQRTLYLELYLMESGTLATRMVDALCAAAH
CCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCHHHHHHHHHHHHHH
RGVTVRVLLDGYGSMKLSHADRQRLRDAGVALRFFNPLSWHRLGGNLSRDHRKLLVVDDR
CCCEEEEEECCCCCEEECHHHHHHHHHHCEEEEECCCCHHHHCCCCCCCCCCEEEEEECC
LAFTGGFGAVDAFVDAWYEVAVRIEGPVVADWIGLFAQLWESPLTRGRAARCRWPEPAPI
EEEECCCHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
AALPDDMRGRMISGRGHRYQAIRLSLHQRIATAERRLWLCTPYFMPTFSLRRRLVRAARL
CCCCCHHCCCEECCCCCEEHHHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHC
GVDVRLLLPGSRHDHPGIRYAGQRFYARLLRAGVRIYEFQPTFIHAKFVLADDWTSIGSC
CCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCEEEEEEEEEECCCCCCCCC
NFDHWSLQWNLEANQEVEDVRFARDVAALFERNFAASEEIHWAQWRRRPWMQRCREWVFG
CCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHHHHHHHHHHHH
TLNGWMTRLR
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377 [H]