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The map label for this gene is gcvA [H]

Identifier: 92112797

GI number: 92112797

Start: 743600

End: 744514

Strand: Direct

Name: gcvA [H]

Synonym: Csal_0665

Alternate gene names: 92112797

Gene position: 743600-744514 (Clockwise)

Preceding gene: 92112795

Following gene: 92112801

Centisome position: 20.12

GC content: 65.57

Gene sequence:

>915_bases
ATGACGGCACGCCACCTGCCCTCGACCATCACCATGCAATGCTTCGAGGCGGCTGCACGGCATATGAGTTTCACACGTGC
CGCCGACGAACTGAGCATTACCCAGAGCGCCATCAGCAAGCAAGTGGCGCAACTCGAAGCGTATCTGCAGCACAAGCTGT
TCCGGCGTGTGCGCCGCACCCTGGTGCTGACGCCGGAGGGCGCGCTGTACCTTACTGAAGTACGCAAGATTCTGGCCCGG
ATCGAGATGTCAGCGAACGCGATCATGAGCTACAGCGGGCAGGGTGAGGTGCTGCGGGTGGCCTGCCTGCCGACCTTCGG
GGCCCGCTGGCTGGCCCGCCAGTTGCCCGACTTCCTGAACGCCAACCCGCACATCGACCTCAGCGTTAGCGACCATGTCG
AGCCCTTCGACCTGGATCAGGCAGGTATCGATGTGGCTGTCTTCCACGGGCATGGTCGCTGGCCGAAACTGGAGTGCCAC
AAGCTGTTCGACGAGGAGGTGGTCGCGGTCGGCGCACCGGACTACCTGGCCAGACGGCAACCGACGAGCGCCGCCGACCT
GGCCGAGTGCCGCCTGCTGCACCTCTCCACGCGCCCGGATGCCTGGCACCGCTGGTTCGCCGACCAGGGCATCACCACCG
AGCGCAGTTACCATGGCACTCGCTTCGAGACCTTCCAGATGCTGATCCGCACGGCGATGAGCGGCGGTGGTCTCGCCCTG
GTACCGCGTTTCTTCGTCGACACCGAGCTGGCCGAAGGCCGCCTGTCCCTGGCCTGGCCGCACGTCCTCAAGGGACCCGA
CGCCTACTACCTGGTGCACCCCGAGCATCTCGGCGAACTCGTGCGAGTGCGCCGTTTCGTCACTCATGTGCTGACGTGTG
CCGAGGTGGATGCGCCAGCCGGGGCGTCGTGCTAG

Upstream 100 bases:

>100_bases
TTCCTTTAACTCATGTATTGCGCTTGAATGCTTTAAATAAAGCCTGGGCATGAGATTTCAGCATGAGCGTTGCGAATTCA
TTCCGTCGCGGAGAAGACGC

Downstream 100 bases:

>100_bases
TGCCTGCCGCCGACGCTTCCGGCGGCAGGCACCACGGCTTTCAATCAGCGCTCACTTCGGCGGTTCGACGTGGCCGCCGA
AACCGAAGCGCATGGCGGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTARHLPSTITMQCFEAAARHMSFTRAADELSITQSAISKQVAQLEAYLQHKLFRRVRRTLVLTPEGALYLTEVRKILAR
IEMSANAIMSYSGQGEVLRVACLPTFGARWLARQLPDFLNANPHIDLSVSDHVEPFDLDQAGIDVAVFHGHGRWPKLECH
KLFDEEVVAVGAPDYLARRQPTSAADLAECRLLHLSTRPDAWHRWFADQGITTERSYHGTRFETFQMLIRTAMSGGGLAL
VPRFFVDTELAEGRLSLAWPHVLKGPDAYYLVHPEHLGELVRVRRFVTHVLTCAEVDAPAGASC

Sequences:

>Translated_304_residues
MTARHLPSTITMQCFEAAARHMSFTRAADELSITQSAISKQVAQLEAYLQHKLFRRVRRTLVLTPEGALYLTEVRKILAR
IEMSANAIMSYSGQGEVLRVACLPTFGARWLARQLPDFLNANPHIDLSVSDHVEPFDLDQAGIDVAVFHGHGRWPKLECH
KLFDEEVVAVGAPDYLARRQPTSAADLAECRLLHLSTRPDAWHRWFADQGITTERSYHGTRFETFQMLIRTAMSGGGLAL
VPRFFVDTELAEGRLSLAWPHVLKGPDAYYLVHPEHLGELVRVRRFVTHVLTCAEVDAPAGASC
>Mature_303_residues
TARHLPSTITMQCFEAAARHMSFTRAADELSITQSAISKQVAQLEAYLQHKLFRRVRRTLVLTPEGALYLTEVRKILARI
EMSANAIMSYSGQGEVLRVACLPTFGARWLARQLPDFLNANPHIDLSVSDHVEPFDLDQAGIDVAVFHGHGRWPKLECHK
LFDEEVVAVGAPDYLARRQPTSAADLAECRLLHLSTRPDAWHRWFADQGITTERSYHGTRFETFQMLIRTAMSGGGLALV
PRFFVDTELAEGRLSLAWPHVLKGPDAYYLVHPEHLGELVRVRRFVTHVLTCAEVDAPAGASC

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=34.8122866894198, Blast_Score=171, Evalue=4e-44,
Organism=Escherichia coli, GI1788706, Length=251, Percent_Identity=32.2709163346614, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI1786448, Length=270, Percent_Identity=30, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789639, Length=269, Percent_Identity=28.996282527881, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI145693193, Length=280, Percent_Identity=27.5, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786401, Length=299, Percent_Identity=26.7558528428094, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787589, Length=270, Percent_Identity=26.2962962962963, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI157672245, Length=201, Percent_Identity=29.8507462686567, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790262, Length=251, Percent_Identity=27.4900398406374, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790399, Length=245, Percent_Identity=26.1224489795918, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34080; Mature: 33949

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARHLPSTITMQCFEAAARHMSFTRAADELSITQSAISKQVAQLEAYLQHKLFRRVRRT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLTPEGALYLTEVRKILARIEMSANAIMSYSGQGEVLRVACLPTFGARWLARQLPDFLN
EEECCCCCCHHHHHHHHHHHHHHCHHHHEEECCCCCEEEEEECCCHHHHHHHHHCHHHHC
ANPHIDLSVSDHVEPFDLDQAGIDVAVFHGHGRWPKLECHKLFDEEVVAVGAPDYLARRQ
CCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCHHEEEECCCHHHHCCC
PTSAADLAECRLLHLSTRPDAWHRWFADQGITTERSYHGTRFETFQMLIRTAMSGGGLAL
CCCHHHHHHHHEEEECCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
VPRFFVDTELAEGRLSLAWPHVLKGPDAYYLVHPEHLGELVRVRRFVTHVLTCAEVDAPA
HHHHHHHHHHCCCEEEEECCHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCC
GASC
CCCC
>Mature Secondary Structure 
TARHLPSTITMQCFEAAARHMSFTRAADELSITQSAISKQVAQLEAYLQHKLFRRVRRT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVLTPEGALYLTEVRKILARIEMSANAIMSYSGQGEVLRVACLPTFGARWLARQLPDFLN
EEECCCCCCHHHHHHHHHHHHHHCHHHHEEECCCCCEEEEEECCCHHHHHHHHHCHHHHC
ANPHIDLSVSDHVEPFDLDQAGIDVAVFHGHGRWPKLECHKLFDEEVVAVGAPDYLARRQ
CCCCEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCHHEEEECCCHHHHCCC
PTSAADLAECRLLHLSTRPDAWHRWFADQGITTERSYHGTRFETFQMLIRTAMSGGGLAL
CCCHHHHHHHHEEEECCCCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEE
VPRFFVDTELAEGRLSLAWPHVLKGPDAYYLVHPEHLGELVRVRRFVTHVLTCAEVDAPA
HHHHHHHHHHCCCEEEEECCHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCC
GASC
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]