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The map label for this gene is gltD [H]

Identifier: 92112749

GI number: 92112749

Start: 689118

End: 690536

Strand: Direct

Name: gltD [H]

Synonym: Csal_0616

Alternate gene names: 92112749

Gene position: 689118-690536 (Clockwise)

Preceding gene: 92112748

Following gene: 92112750

Centisome position: 18.64

GC content: 65.33

Gene sequence:

>1419_bases
ATGGCAAACCGTCTGAATAACGATTTCCAGTTCATCGATGTCGGCCGTCAGGACCCGGAGAAGAAGCAAGCGCGTGCCCG
CGCCCGCGAATTCGCCGAGATCTACGAGCCCTACAAGCCCAGCGAGGCGGCCAGCCAGGCGCATCGCTGCCTGCACTGCG
GCAATCCGTACTGCGAGTGGAAGTGCCCGGTGCACAACTACATTCCCAACTGGCTCAAGCTGGTCAGCGAGGGCAACATC
CTGGAAGCGGCGGAGATGTCTCACCGCACCAACTCGCTGCCCGAGGTCTGCGGGCGGGTGTGCCCTCAGGACCGTCTCTG
CGAGGGCGACTGCACGCTCAACGACGGTTTCGGTGCGGTCACCATCGGTTCGGTGGAGAAATACATCACCGACACGGCCT
TCGCCATGGGGTGGCGGCCGGACATGTCCAAGGTCGAGTGGACCGACAAGCGCGTCGCGGTGATCGGCGCCGGTCCGGCA
GGGCTGGGGTGCGCCGACATTCTGGTGCGCAACGGCGTCAAGCCGGTGGTGTTCGACAAGCACCCCGAGATCGGCGGATT
GCTGACCTTCGGGATTCCCGAGTTCAAGCTCGAGAAGCACGTCATGAAGCGCCGCCGCGCGGTGTTCGAGGAGATGGGCG
TCGAGTTCCGCCTGGGCGTGGAGATCGGGCGCGACGTCACGCATGACGCGCTGCTCGACGAGTTCGATGCCGTCTTCATG
GGCATGGGCACCTACAAGTACATGGAAGGCGGTTTTCCCGGCGAGGATCTGCCCGGTGTGCACAAGGCGCTGGATTTCCT
GATCGCCAACGTCAATCACTGCCTGGGCTTCGAGACGAATCCCGACGACTACGTCTCGCTCGAGGGCAAGCGCGTCGTGG
TCCTGGGCGGCGGCGATACGGCGATGGACTGCAACCGCACCTCGATTCGTCAGGGCGCGACCAGTGTGACCTGTGCCTAC
CGCCGCGACGAGGAGAACATGCCGGGCTCCAAGCGCGAGGTGGCCAACGCCCGCGAAGAGGGTGTGGAGTTCCTGTTCAA
TCGTCAGCCGGTGGCCATCGTCGGCGAAGACAAGGTCGAAGGCGTCAAGCTGGTGCGCACGCGGCTCGGCGAGCCGGATG
CCAACGGCCGTCAGCGGCCCGAGGTGGTGCCGGGTTCGGAGGAAGTGTTCGCGGCCGACGCCGTGATCATCGCCTTCGGC
TTCCAGCCCAGCCCCGCCGACTGGTTCGACACGGTGGGCATCGACCTCGACGAGCGCGGGCGCGTCAAGGCACCGGCCCA
GGCAAGTTTCGCCTTCCAGACCAGCCATGAAAAGATCTTCGCCGGTGGCGACATGGTGCGTGGCTCCGACCTGGTGGTCA
CCGCGGTCTATGAAGGCCGCCAGGCGGGCGAGGGCATCCTCGACTACCTCGGCGTCTGA

Upstream 100 bases:

>100_bases
TGGCTGGTGAAGCCCAAGGCGGCGAGCCTCAACGGCCTGCTGGCCGAATCCCGTCGGCGGCCCGAATGAGCCGGACTTCC
AGGTACCAGGAGAGACGATC

Downstream 100 bases:

>100_bases
GTCTCGAGCTTCGGCTGTCGGGCAGGACGGAGACATCATCCTGCCCGCAGCCCGCAGCCCGCAGCCCGCAGCCCGCAGCC
CGCAGCCCGCAGCCCGCAGC

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MANRLNNDFQFIDVGRQDPEKKQARARAREFAEIYEPYKPSEAASQAHRCLHCGNPYCEWKCPVHNYIPNWLKLVSEGNI
LEAAEMSHRTNSLPEVCGRVCPQDRLCEGDCTLNDGFGAVTIGSVEKYITDTAFAMGWRPDMSKVEWTDKRVAVIGAGPA
GLGCADILVRNGVKPVVFDKHPEIGGLLTFGIPEFKLEKHVMKRRRAVFEEMGVEFRLGVEIGRDVTHDALLDEFDAVFM
GMGTYKYMEGGFPGEDLPGVHKALDFLIANVNHCLGFETNPDDYVSLEGKRVVVLGGGDTAMDCNRTSIRQGATSVTCAY
RRDEENMPGSKREVANAREEGVEFLFNRQPVAIVGEDKVEGVKLVRTRLGEPDANGRQRPEVVPGSEEVFAADAVIIAFG
FQPSPADWFDTVGIDLDERGRVKAPAQASFAFQTSHEKIFAGGDMVRGSDLVVTAVYEGRQAGEGILDYLGV

Sequences:

>Translated_472_residues
MANRLNNDFQFIDVGRQDPEKKQARARAREFAEIYEPYKPSEAASQAHRCLHCGNPYCEWKCPVHNYIPNWLKLVSEGNI
LEAAEMSHRTNSLPEVCGRVCPQDRLCEGDCTLNDGFGAVTIGSVEKYITDTAFAMGWRPDMSKVEWTDKRVAVIGAGPA
GLGCADILVRNGVKPVVFDKHPEIGGLLTFGIPEFKLEKHVMKRRRAVFEEMGVEFRLGVEIGRDVTHDALLDEFDAVFM
GMGTYKYMEGGFPGEDLPGVHKALDFLIANVNHCLGFETNPDDYVSLEGKRVVVLGGGDTAMDCNRTSIRQGATSVTCAY
RRDEENMPGSKREVANAREEGVEFLFNRQPVAIVGEDKVEGVKLVRTRLGEPDANGRQRPEVVPGSEEVFAADAVIIAFG
FQPSPADWFDTVGIDLDERGRVKAPAQASFAFQTSHEKIFAGGDMVRGSDLVVTAVYEGRQAGEGILDYLGV
>Mature_471_residues
ANRLNNDFQFIDVGRQDPEKKQARARAREFAEIYEPYKPSEAASQAHRCLHCGNPYCEWKCPVHNYIPNWLKLVSEGNIL
EAAEMSHRTNSLPEVCGRVCPQDRLCEGDCTLNDGFGAVTIGSVEKYITDTAFAMGWRPDMSKVEWTDKRVAVIGAGPAG
LGCADILVRNGVKPVVFDKHPEIGGLLTFGIPEFKLEKHVMKRRRAVFEEMGVEFRLGVEIGRDVTHDALLDEFDAVFMG
MGTYKYMEGGFPGEDLPGVHKALDFLIANVNHCLGFETNPDDYVSLEGKRVVVLGGGDTAMDCNRTSIRQGATSVTCAYR
RDEENMPGSKREVANAREEGVEFLFNRQPVAIVGEDKVEGVKLVRTRLGEPDANGRQRPEVVPGSEEVFAADAVIIAFGF
QPSPADWFDTVGIDLDERGRVKAPAQASFAFQTSHEKIFAGGDMVRGSDLVVTAVYEGRQAGEGILDYLGV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=462, Percent_Identity=28.5714285714286, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI111118983, Length=249, Percent_Identity=28.1124497991968, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI111118981, Length=158, Percent_Identity=31.6455696202532, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1789606, Length=470, Percent_Identity=67.2340425531915, Blast_Score=657, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=462, Percent_Identity=58.2251082251082, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI87082180, Length=456, Percent_Identity=55.7017543859649, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI1788468, Length=441, Percent_Identity=30.6122448979592, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789243, Length=420, Percent_Identity=31.6666666666667, Blast_Score=142, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17570289, Length=490, Percent_Identity=33.0612244897959, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI71984108, Length=459, Percent_Identity=29.8474945533769, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17543860, Length=160, Percent_Identity=32.5, Blast_Score=78, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320030, Length=493, Percent_Identity=31.237322515213, Blast_Score=216, Evalue=9e-57,
Organism=Drosophila melanogaster, GI28574881, Length=482, Percent_Identity=36.7219917012448, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24665539, Length=482, Percent_Identity=36.7219917012448, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI24665547, Length=482, Percent_Identity=36.7219917012448, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24665543, Length=482, Percent_Identity=36.7219917012448, Blast_Score=278, Evalue=5e-75,
Organism=Drosophila melanogaster, GI24640763, Length=459, Percent_Identity=28.1045751633987, Blast_Score=145, Evalue=6e-35,
Organism=Drosophila melanogaster, GI18858217, Length=459, Percent_Identity=28.1045751633987, Blast_Score=145, Evalue=6e-35,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52020; Mature: 51889

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLNNDFQFIDVGRQDPEKKQARARAREFAEIYEPYKPSEAASQAHRCLHCGNPYCEW
CCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEE
KCPVHNYIPNWLKLVSEGNILEAAEMSHRTNSLPEVCGRVCPQDRLCEGDCTLNDGFGAV
ECCHHHHCHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEE
TIGSVEKYITDTAFAMGWRPDMSKVEWTDKRVAVIGAGPAGLGCADILVRNGVKPVVFDK
EECCHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCCEEECC
HPEIGGLLTFGIPEFKLEKHVMKRRRAVFEEMGVEFRLGVEIGRDVTHDALLDEFDAVFM
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
GMGTYKYMEGGFPGEDLPGVHKALDFLIANVNHCLGFETNPDDYVSLEGKRVVVLGGGDT
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCC
AMDCNRTSIRQGATSVTCAYRRDEENMPGSKREVANAREEGVEFLFNRQPVAIVGEDKVE
CCCCCHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
GVKLVRTRLGEPDANGRQRPEVVPGSEEVFAADAVIIAFGFQPSPADWFDTVGIDLDERG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCHHHHCCCCCCCCC
RVKAPAQASFAFQTSHEKIFAGGDMVRGSDLVVTAVYEGRQAGEGILDYLGV
CCCCCCCCCEEEECCCCEEEECCCEECCCCEEEEEEECCCCCCCHHHHHHCC
>Mature Secondary Structure 
ANRLNNDFQFIDVGRQDPEKKQARARAREFAEIYEPYKPSEAASQAHRCLHCGNPYCEW
CCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEE
KCPVHNYIPNWLKLVSEGNILEAAEMSHRTNSLPEVCGRVCPQDRLCEGDCTLNDGFGAV
ECCHHHHCHHHHHHHCCCCEEHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCEECCCCCEE
TIGSVEKYITDTAFAMGWRPDMSKVEWTDKRVAVIGAGPAGLGCADILVRNGVKPVVFDK
EECCHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCCCCCHHHHHHHCCCCCEEECC
HPEIGGLLTFGIPEFKLEKHVMKRRRAVFEEMGVEFRLGVEIGRDVTHDALLDEFDAVFM
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
GMGTYKYMEGGFPGEDLPGVHKALDFLIANVNHCLGFETNPDDYVSLEGKRVVVLGGGDT
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCEEEEECCCCC
AMDCNRTSIRQGATSVTCAYRRDEENMPGSKREVANAREEGVEFLFNRQPVAIVGEDKVE
CCCCCHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
GVKLVRTRLGEPDANGRQRPEVVPGSEEVFAADAVIIAFGFQPSPADWFDTVGIDLDERG
HHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCEEEEEECCCCCCCCHHHHCCCCCCCCC
RVKAPAQASFAFQTSHEKIFAGGDMVRGSDLVVTAVYEGRQAGEGILDYLGV
CCCCCCCCCEEEECCCCEEEECCCEECCCCEEEEEEECCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]