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The map label for this gene is metF [H]

Identifier: 92112529

GI number: 92112529

Start: 442287

End: 443135

Strand: Direct

Name: metF [H]

Synonym: Csal_0396

Alternate gene names: 92112529

Gene position: 442287-443135 (Clockwise)

Preceding gene: 92112528

Following gene: 92112530

Centisome position: 11.96

GC content: 66.67

Gene sequence:

>849_bases
ATGAGCACTCGCGAGCATCCGCTGGGCGTCAGCTTCGAATTCTTTCCACCCAGCACCGAGGCGGGACGGGAAAAGCTGAA
AGGCGTGCGCGACACGCTGGCCGAGCGGCGTCCGCGTTTCTTTTCGGTGACCTACGGCGCCGGTGGCTCGACGCAGGCAC
GCACCTTCGAGACCGTGCAGACGGTGCGCCAGAGCGGCATCGACACCGCGCCGCACCTGTCCTGCATCGGCAGCGACAAG
GGCGAGTTGCGCGAGCGCCTGGCGAGTTTTCGCGAGGCGGGCATCGACAGCCTGGTGGCGCTGCGCGGCGACTTGCCTTC
CGGCATGGGGGGGATGGGCGAGCTGCGCTATGCCAACGAGCTGGTCGAGTTCATCCGCGAGGAAACCGGTGACCATTTCC
AGATCGCGGTCGCGGCCTATCCCGAAGCCCACCCCCAGGCCCCCAGCTACGAGCACGACCTGCAGAACTTCGTGCGCAAG
ATGCAGGCCGGGGCCGATCTGGCCATCACCCAGTATTTCTTCAGCGCCGACGCCTACTTCCACTTCGTCGAGCGTGCCCG
GGCACTGGGCGTCGAGGCGCCGATCGTGCCGGGCATCATGCCGATCACCAATTACACCAAGCTGGCGCGCTTTTCCGACG
CGTGCGGCGCGGACATTCCCCGCTGGATCCGCAAGCAGCTGGAGGCCTACGGCGATGACAGCGAGAGCATCCAGGCGTTC
GGCAGCGAGGTCGTCACGCGCCTCTGCCAGCGGTTGCTCGATGGCGGTGCGCCGGGGTTGCACTTCTATACACTGAACCA
GGCGACGCCGGCGCTGCGGGTGCTCGACAACCTGCGCTGCGATACCTGA

Upstream 100 bases:

>100_bases
CTTTCATTGGTCAGCGCGGGTGGGGCACGGCAGAATGCGCGGCATCCATTAGTCACGTGACCCGATGCCGGTCGCGCCAT
GTCACGAAGAGGGTTGTACG

Downstream 100 bases:

>100_bases
GTCATGGCGCCGTTCGCAGCTGTCGTCTAGCCTGACACACGTAGACGTCTCACTCATGGACAGGAGAAAGGCATGCTACG
TATCTCGCAGATGGCACTCG

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MSTREHPLGVSFEFFPPSTEAGREKLKGVRDTLAERRPRFFSVTYGAGGSTQARTFETVQTVRQSGIDTAPHLSCIGSDK
GELRERLASFREAGIDSLVALRGDLPSGMGGMGELRYANELVEFIREETGDHFQIAVAAYPEAHPQAPSYEHDLQNFVRK
MQAGADLAITQYFFSADAYFHFVERARALGVEAPIVPGIMPITNYTKLARFSDACGADIPRWIRKQLEAYGDDSESIQAF
GSEVVTRLCQRLLDGGAPGLHFYTLNQATPALRVLDNLRCDT

Sequences:

>Translated_282_residues
MSTREHPLGVSFEFFPPSTEAGREKLKGVRDTLAERRPRFFSVTYGAGGSTQARTFETVQTVRQSGIDTAPHLSCIGSDK
GELRERLASFREAGIDSLVALRGDLPSGMGGMGELRYANELVEFIREETGDHFQIAVAAYPEAHPQAPSYEHDLQNFVRK
MQAGADLAITQYFFSADAYFHFVERARALGVEAPIVPGIMPITNYTKLARFSDACGADIPRWIRKQLEAYGDDSESIQAF
GSEVVTRLCQRLLDGGAPGLHFYTLNQATPALRVLDNLRCDT
>Mature_281_residues
STREHPLGVSFEFFPPSTEAGREKLKGVRDTLAERRPRFFSVTYGAGGSTQARTFETVQTVRQSGIDTAPHLSCIGSDKG
ELRERLASFREAGIDSLVALRGDLPSGMGGMGELRYANELVEFIREETGDHFQIAVAAYPEAHPQAPSYEHDLQNFVRKM
QAGADLAITQYFFSADAYFHFVERARALGVEAPIVPGIMPITNYTKLARFSDACGADIPRWIRKQLEAYGDDSESIQAFG
SEVVTRLCQRLLDGGAPGLHFYTLNQATPALRVLDNLRCDT

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=277, Percent_Identity=38.2671480144404, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1790377, Length=262, Percent_Identity=39.6946564885496, Blast_Score=208, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI25147436, Length=277, Percent_Identity=35.0180505415162, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI25147441, Length=277, Percent_Identity=35.0180505415162, Blast_Score=178, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6321313, Length=278, Percent_Identity=33.0935251798561, Blast_Score=171, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6325234, Length=277, Percent_Identity=33.5740072202166, Blast_Score=149, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24662658, Length=229, Percent_Identity=27.5109170305677, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 31157; Mature: 31026

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTREHPLGVSFEFFPPSTEAGREKLKGVRDTLAERRPRFFSVTYGAGGSTQARTFETVQ
CCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
TVRQSGIDTAPHLSCIGSDKGELRERLASFREAGIDSLVALRGDLPSGMGGMGELRYANE
HHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHH
LVEFIREETGDHFQIAVAAYPEAHPQAPSYEHDLQNFVRKMQAGADLAITQYFFSADAYF
HHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
HFVERARALGVEAPIVPGIMPITNYTKLARFSDACGADIPRWIRKQLEAYGDDSESIQAF
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
GSEVVTRLCQRLLDGGAPGLHFYTLNQATPALRVLDNLRCDT
HHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
STREHPLGVSFEFFPPSTEAGREKLKGVRDTLAERRPRFFSVTYGAGGSTQARTFETVQ
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHH
TVRQSGIDTAPHLSCIGSDKGELRERLASFREAGIDSLVALRGDLPSGMGGMGELRYANE
HHHHCCCCCCCCHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHHHHHH
LVEFIREETGDHFQIAVAAYPEAHPQAPSYEHDLQNFVRKMQAGADLAITQYFFSADAYF
HHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
HFVERARALGVEAPIVPGIMPITNYTKLARFSDACGADIPRWIRKQLEAYGDDSESIQAF
HHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHH
GSEVVTRLCQRLLDGGAPGLHFYTLNQATPALRVLDNLRCDT
HHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]