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The map label for this gene is ubiF [H]

Identifier: 92112509

GI number: 92112509

Start: 420193

End: 421428

Strand: Direct

Name: ubiF [H]

Synonym: Csal_0376

Alternate gene names: 92112509

Gene position: 420193-421428 (Clockwise)

Preceding gene: 92112508

Following gene: 92112510

Centisome position: 11.37

GC content: 70.31

Gene sequence:

>1236_bases
ATGCCGCAACCTGCCGGAGGTATCGCGATCAAGGATGTCGTTGACCAGGATGTCGTTGCCGAGAACGTCGCGACCCGGGA
TGTCGCCATCGTCGGCGCGGGGCCGGTCGGACTGTGCCTGGCGCTGGAGCTGGCGGCGCTTGGCCTGACGGTGACGGTGA
TCGATCGGGCGCCCTGGAAGGCGCTGGCGTCGCCCGATGACGATGGACGCGAGATCGCCCTGACGCATGCCTCGCGCAGG
CAGCTGGCGGCGGCGGGCATCGCCATTCCCGAGGACCGCATGGCGCCGATTCGCGAGGCGCGCCTCTACAATGGCGATCC
GCCCCATGGCATGACGCTGGCCAGTGCGCGGGGCGAGGCGCAGGACGGCATCGGCCAGCTCGTCGCCAATCACCTCATTC
GACGTGCCGCGTTCGAGGCCGTGCGAGACGTCGACCGTATCGAGCTCATCACCGGGCACGGCGTGCAAGCGCTGAACGGT
GGCGCCGACTGGCGTAGCGTACGGCTCGATGACCAGCGCGACATTCCGGCGCGCCTGGTGGTGGCCGCGGACGGGCGCCT
CTCGGTCACCCGCCGTCTGGCGGGACTTTCCGCGCAGATTCACGATACCGGTCAACGCCTGCTGGTGCGTCGCATGGCGC
ATGAGCGTTCCCATCGGCAGACGACATGGTCATGGTTCCACGGCGAACGCGGCCTGGCATTGCTGCCGCTGGCAGGGGAC
ACCGCGTCGTTGGTGATGACGCTGCCCCCGGAGGATGCCGAGCGTCTCGAGGCCATGTCGCCGACGGCGTTCGCGGCCGA
GGCGACACGGCTTTATCGGGGCTGTGTGGGCGAGATGACGTGCCTCGATGCGCTGCACGATTATCCGCTCACGGTCAGCC
ACGCCGACCGCCTGGTGGCCGATCGCGTGGCCCTGGTGGGCGACGCGGCCCTGGGCCTGCACCCCGTCACGGCACACGGC
TTCAATGTGGGGCTGGCCAGCGTACGTCGCCTGGTCGAGGCGCTGGCGAGTCACGCCGGGACGACGGCAGATGTCGGCGC
AGCGGCGGGCCTGGCGAGCTATGCGCGTCACCACCAGCGCGCGATCGTGCCGCTGTGGCATGCCTCGCGTGCTCTGGTGG
CGCTGTACGGCGATACGCGGCCGCCGGCGCGTCTGGCGCGTCACCTGGCCCTGGGCGCCGCGCGTCGGATCGCGCCGCTG
CAGTGGCTGATGGAAGGCTATCTGGCGCGGCACTGA

Upstream 100 bases:

>100_bases
AAGGTCGCGACCCATTGTCACGCCTGTGTCGCGCGAGCGTCGTGCCGATTATGATGGCGGCGTGTCCGATCGGGGCACGC
TCATTCGATGCGGAGGGCGC

Downstream 100 bases:

>100_bases
GGCATCTCACCCACCTGTCGGGCGCCTGGTTGACGACGGGACGTGACGCGTACCGAATTCTCGCTCCGTCGTCAATATCT
ATGCGAACCTCCCAGTTTGT

Product: hypothetical protein

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 410

Protein sequence:

>411_residues
MPQPAGGIAIKDVVDQDVVAENVATRDVAIVGAGPVGLCLALELAALGLTVTVIDRAPWKALASPDDDGREIALTHASRR
QLAAAGIAIPEDRMAPIREARLYNGDPPHGMTLASARGEAQDGIGQLVANHLIRRAAFEAVRDVDRIELITGHGVQALNG
GADWRSVRLDDQRDIPARLVVAADGRLSVTRRLAGLSAQIHDTGQRLLVRRMAHERSHRQTTWSWFHGERGLALLPLAGD
TASLVMTLPPEDAERLEAMSPTAFAAEATRLYRGCVGEMTCLDALHDYPLTVSHADRLVADRVALVGDAALGLHPVTAHG
FNVGLASVRRLVEALASHAGTTADVGAAAGLASYARHHQRAIVPLWHASRALVALYGDTRPPARLARHLALGAARRIAPL
QWLMEGYLARH

Sequences:

>Translated_411_residues
MPQPAGGIAIKDVVDQDVVAENVATRDVAIVGAGPVGLCLALELAALGLTVTVIDRAPWKALASPDDDGREIALTHASRR
QLAAAGIAIPEDRMAPIREARLYNGDPPHGMTLASARGEAQDGIGQLVANHLIRRAAFEAVRDVDRIELITGHGVQALNG
GADWRSVRLDDQRDIPARLVVAADGRLSVTRRLAGLSAQIHDTGQRLLVRRMAHERSHRQTTWSWFHGERGLALLPLAGD
TASLVMTLPPEDAERLEAMSPTAFAAEATRLYRGCVGEMTCLDALHDYPLTVSHADRLVADRVALVGDAALGLHPVTAHG
FNVGLASVRRLVEALASHAGTTADVGAAAGLASYARHHQRAIVPLWHASRALVALYGDTRPPARLARHLALGAARRIAPL
QWLMEGYLARH
>Mature_410_residues
PQPAGGIAIKDVVDQDVVAENVATRDVAIVGAGPVGLCLALELAALGLTVTVIDRAPWKALASPDDDGREIALTHASRRQ
LAAAGIAIPEDRMAPIREARLYNGDPPHGMTLASARGEAQDGIGQLVANHLIRRAAFEAVRDVDRIELITGHGVQALNGG
ADWRSVRLDDQRDIPARLVVAADGRLSVTRRLAGLSAQIHDTGQRLLVRRMAHERSHRQTTWSWFHGERGLALLPLAGDT
ASLVMTLPPEDAERLEAMSPTAFAAEATRLYRGCVGEMTCLDALHDYPLTVSHADRLVADRVALVGDAALGLHPVTAHGF
NVGLASVRRLVEALASHAGTTADVGAAAGLASYARHHQRAIVPLWHASRALVALYGDTRPPARLARHLALGAARRIAPLQ
WLMEGYLARH

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=323, Percent_Identity=24.7678018575851, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI32967307, Length=323, Percent_Identity=24.7678018575851, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786883, Length=378, Percent_Identity=29.1005291005291, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789273, Length=377, Percent_Identity=28.9124668435013, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789274, Length=379, Percent_Identity=27.4406332453826, Blast_Score=103, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321694, Length=447, Percent_Identity=25.2796420581655, Blast_Score=80, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 43899; Mature: 43768

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQPAGGIAIKDVVDQDVVAENVATRDVAIVGAGPVGLCLALELAALGLTVTVIDRAPWK
CCCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCEEEEEECCCCCH
ALASPDDDGREIALTHASRRQLAAAGIAIPEDRMAPIREARLYNGDPPHGMTLASARGEA
HCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHCCHHHHHHCCCCCCCCCEEEECCCCCH
QDGIGQLVANHLIRRAAFEAVRDVDRIELITGHGVQALNGGADWRSVRLDDQRDIPARLV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCEEEECCCCCCCCEEEE
VAADGRLSVTRRLAGLSAQIHDTGQRLLVRRMAHERSHRQTTWSWFHGERGLALLPLAGD
EEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCEEEEECCC
TASLVMTLPPEDAERLEAMSPTAFAAEATRLYRGCVGEMTCLDALHDYPLTVSHADRLVA
CEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DRVALVGDAALGLHPVTAHGFNVGLASVRRLVEALASHAGTTADVGAAAGLASYARHHQR
HHHHHHCCHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
AIVPLWHASRALVALYGDTRPPARLARHLALGAARRIAPLQWLMEGYLARH
EEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PQPAGGIAIKDVVDQDVVAENVATRDVAIVGAGPVGLCLALELAALGLTVTVIDRAPWK
CCCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHCEEEEEECCCCCH
ALASPDDDGREIALTHASRRQLAAAGIAIPEDRMAPIREARLYNGDPPHGMTLASARGEA
HCCCCCCCCCEEEEEHHHHHHHHHHCCCCCCHHCCHHHHHHCCCCCCCCCEEEECCCCCH
QDGIGQLVANHLIRRAAFEAVRDVDRIELITGHGVQALNGGADWRSVRLDDQRDIPARLV
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEECCCCCCCEEEECCCCCCCCEEEE
VAADGRLSVTRRLAGLSAQIHDTGQRLLVRRMAHERSHRQTTWSWFHGERGLALLPLAGD
EEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHEEECCCCCEEEEECCC
TASLVMTLPPEDAERLEAMSPTAFAAEATRLYRGCVGEMTCLDALHDYPLTVSHADRLVA
CEEEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DRVALVGDAALGLHPVTAHGFNVGLASVRRLVEALASHAGTTADVGAAAGLASYARHHQR
HHHHHHCCHHHCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCC
AIVPLWHASRALVALYGDTRPPARLARHLALGAARRIAPLQWLMEGYLARH
EEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]