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The map label for this gene is pcaB [H]

Identifier: 92112432

GI number: 92112432

Start: 336726

End: 338060

Strand: Direct

Name: pcaB [H]

Synonym: Csal_0298

Alternate gene names: 92112432

Gene position: 336726-338060 (Clockwise)

Preceding gene: 92112428

Following gene: 92112433

Centisome position: 9.11

GC content: 62.47

Gene sequence:

>1335_bases
GTGTACGAGAAACTTTTACGCCACCCGTTTACCAGCGCCACCGGCCGAGAAAAGTGCAGTGCCCAGGATCTCGTCGTGGC
AATGCTCGACGTGGAACTGGCGCTAGCCGCCGAGCAAGAGCGTCTAGATATGCTGCCCAAGGGCACTACCGAGACGCTTG
GTGAGTATCTGACCACTGAGGTCTTCGATATCGAGGCACTAGCGGACGACACCGCCAAGGGCGGCAATGCGGCGATTCCC
TTCGTCAAGCAGGCCAAGGCGGCCCTTCCCGATACATTGAAGCCGTATTTCCATCGTGGGGCAACCAGCCAGGACATCGT
CGATAGCGCCTTGATGCTCTTGCTCAAGCCGCGGCTGGCACGCTGCCGGCGTCTGCTGACGGCGTCCATCGACGATGGTC
GGTCGTTGATGGTGGCGCATCGGGATACGACGATGATCGGGCGCACGCTGATGCAGCAGGCGTTGCCCATCACCTTCGGA
GCCAAGGTAGGGCAATGGGCGTGGGGGCTACACCAGGCGGGTGAACGCTTGGACGATGTGTTGACCGGTGGGCTCTATGT
GCAGTTCGGAGGGGCCGTGGGCGTGCATTCAGGCCTGGATGACGCCGGGGTCGCGATCATGACTGGCATGGCGAGTCGTT
TGGGGCTCGCTGAGCCATGGCTACCTTGGCATACCGATCGCCAGCCGATTCATTCGTTGGGGTGTGCGATCGATGCCGTA
GCCGTGGCCGCCGAGAAGGTCGCCCTCGACGTCGCGCTTCTCACCCAGACGGAGATTGGTGAGCTGGCTGAGCCTGCCGA
GGCAGGCGTTGGTGAATCGTCGTCTATGCCGCATAAGCGCAACCCGGTGGGCTGCGCCCGTATCCGTGCGGCGGCTCGCC
AGATTCATGCCGCGCTGAGCGTGTTGACCAATGCCGGTGCTCAGCCACTCGAACGTGGACTGGGAGAGTGGCATGCCGAG
TGGGCTCCGATGCTGGATGCGGCATTGCTGCTCGAGGGAGCTCTCGAGACCTTGCAACATCTATTGAGCGGGCTTGAGGT
TAACGATCAAGTGATGGCGCGCAATCTGGGCGTGACAGGCGGTGCGATCATGGCCGAGCCAGCCGCGCGTGTTCTGCAGA
GTATTCTGTCCAACGACGAAGCCCGTCGTGTCGCCCGCGAGGCCTCGGAAAACGCGCGTCGCGCTGGTGTGGACTATGCC
GAGGCACTGCTGGCCCACGAGGACGTCGCGGGGAGTGCCATTGACGCCGACGCCCTTCGCCAGGCGGTGCGTCCTGAATC
ATACATTGGTAGCAGCCGTTGTCTGGTTGAGCGTGTGATTGAACGCCTGAGATGA

Upstream 100 bases:

>100_bases
TTGTATTGCTCGGTCACTTGGCTGACTCTAGCATAGAGTTCGAATTGCGAACATATGTCCGCTAATGCGTCATTGAGGAT
AAAAACGAGGGAGATGACCA

Downstream 100 bases:

>100_bases
AAGTAAATTATTGTTTTAAAAGTTTTTTATGAAATTTTAGCTGATTTTTCAGCTGAGCGCTGAAACGTCATGCGGTTTGA
CGCGGTAGGCCTACGCAATT

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MYEKLLRHPFTSATGREKCSAQDLVVAMLDVELALAAEQERLDMLPKGTTETLGEYLTTEVFDIEALADDTAKGGNAAIP
FVKQAKAALPDTLKPYFHRGATSQDIVDSALMLLLKPRLARCRRLLTASIDDGRSLMVAHRDTTMIGRTLMQQALPITFG
AKVGQWAWGLHQAGERLDDVLTGGLYVQFGGAVGVHSGLDDAGVAIMTGMASRLGLAEPWLPWHTDRQPIHSLGCAIDAV
AVAAEKVALDVALLTQTEIGELAEPAEAGVGESSSMPHKRNPVGCARIRAAARQIHAALSVLTNAGAQPLERGLGEWHAE
WAPMLDAALLLEGALETLQHLLSGLEVNDQVMARNLGVTGGAIMAEPAARVLQSILSNDEARRVAREASENARRAGVDYA
EALLAHEDVAGSAIDADALRQAVRPESYIGSSRCLVERVIERLR

Sequences:

>Translated_444_residues
MYEKLLRHPFTSATGREKCSAQDLVVAMLDVELALAAEQERLDMLPKGTTETLGEYLTTEVFDIEALADDTAKGGNAAIP
FVKQAKAALPDTLKPYFHRGATSQDIVDSALMLLLKPRLARCRRLLTASIDDGRSLMVAHRDTTMIGRTLMQQALPITFG
AKVGQWAWGLHQAGERLDDVLTGGLYVQFGGAVGVHSGLDDAGVAIMTGMASRLGLAEPWLPWHTDRQPIHSLGCAIDAV
AVAAEKVALDVALLTQTEIGELAEPAEAGVGESSSMPHKRNPVGCARIRAAARQIHAALSVLTNAGAQPLERGLGEWHAE
WAPMLDAALLLEGALETLQHLLSGLEVNDQVMARNLGVTGGAIMAEPAARVLQSILSNDEARRVAREASENARRAGVDYA
EALLAHEDVAGSAIDADALRQAVRPESYIGSSRCLVERVIERLR
>Mature_444_residues
MYEKLLRHPFTSATGREKCSAQDLVVAMLDVELALAAEQERLDMLPKGTTETLGEYLTTEVFDIEALADDTAKGGNAAIP
FVKQAKAALPDTLKPYFHRGATSQDIVDSALMLLLKPRLARCRRLLTASIDDGRSLMVAHRDTTMIGRTLMQQALPITFG
AKVGQWAWGLHQAGERLDDVLTGGLYVQFGGAVGVHSGLDDAGVAIMTGMASRLGLAEPWLPWHTDRQPIHSLGCAIDAV
AVAAEKVALDVALLTQTEIGELAEPAEAGVGESSSMPHKRNPVGCARIRAAARQIHAALSVLTNAGAQPLERGLGEWHAE
WAPMLDAALLLEGALETLQHLLSGLEVNDQVMARNLGVTGGAIMAEPAARVLQSILSNDEARRVAREASENARRAGVDYA
EALLAHEDVAGSAIDADALRQAVRPESYIGSSRCLVERVIERLR

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI17508577, Length=264, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI32564234, Length=256, Percent_Identity=26.953125, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323391, Length=370, Percent_Identity=24.3243243243243, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24662684, Length=395, Percent_Identity=24.5569620253165, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 47542; Mature: 47542

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYEKLLRHPFTSATGREKCSAQDLVVAMLDVELALAAEQERLDMLPKGTTETLGEYLTTE
CCHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VFDIEALADDTAKGGNAAIPFVKQAKAALPDTLKPYFHRGATSQDIVDSALMLLLKPRLA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RCRRLLTASIDDGRSLMVAHRDTTMIGRTLMQQALPITFGAKVGQWAWGLHQAGERLDDV
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LTGGLYVQFGGAVGVHSGLDDAGVAIMTGMASRLGLAEPWLPWHTDRQPIHSLGCAIDAV
HHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
AVAAEKVALDVALLTQTEIGELAEPAEAGVGESSSMPHKRNPVGCARIRAAARQIHAALS
HHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VLTNAGAQPLERGLGEWHAEWAPMLDAALLLEGALETLQHLLSGLEVNDQVMARNLGVTG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
GAIMAEPAARVLQSILSNDEARRVAREASENARRAGVDYAEALLAHEDVAGSAIDADALR
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
QAVRPESYIGSSRCLVERVIERLR
HHHCCHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MYEKLLRHPFTSATGREKCSAQDLVVAMLDVELALAAEQERLDMLPKGTTETLGEYLTTE
CCHHHHCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
VFDIEALADDTAKGGNAAIPFVKQAKAALPDTLKPYFHRGATSQDIVDSALMLLLKPRLA
HHHHHHHHCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
RCRRLLTASIDDGRSLMVAHRDTTMIGRTLMQQALPITFGAKVGQWAWGLHQAGERLDDV
HHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LTGGLYVQFGGAVGVHSGLDDAGVAIMTGMASRLGLAEPWLPWHTDRQPIHSLGCAIDAV
HHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
AVAAEKVALDVALLTQTEIGELAEPAEAGVGESSSMPHKRNPVGCARIRAAARQIHAALS
HHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
VLTNAGAQPLERGLGEWHAEWAPMLDAALLLEGALETLQHLLSGLEVNDQVMARNLGVTG
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCC
GAIMAEPAARVLQSILSNDEARRVAREASENARRAGVDYAEALLAHEDVAGSAIDADALR
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHH
QAVRPESYIGSSRCLVERVIERLR
HHHCCHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063101 [H]