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The map label for this gene is aglA [H]

Identifier: 92112396

GI number: 92112396

Start: 297950

End: 299563

Strand: Direct

Name: aglA [H]

Synonym: Csal_0261

Alternate gene names: 92112396

Gene position: 297950-299563 (Clockwise)

Preceding gene: 92112395

Following gene: 92112397

Centisome position: 8.06

GC content: 59.23

Gene sequence:

>1614_bases
ATGAAGGACAACACTTTCTGGTGGCGCGGTGGTGTCATCTACCAAATCTATCCGCGCAGCTTTCTGGACAGCAACGGCGA
CGGTATCGGCGACCTGCCGGGCATCATCGAAAAGCTCGATTACGTGGCGTCGCTCGGTGTCGATGGCATCTGGCTGTCGC
CTTTCTTTACATCTCCGATGGCGGATTTTGGCTACGACATCAGCGATTATCGCGACGTCGACCCCATGTTCGGCACCCTG
GACGACTTCAAGTCACTGCTCAGTCACGCCCATGCTCTGGGGCTCAAGGTGATCATCGATCAAGTGATCAGCCATACGTC
GGACCAGCATCCCTGGTTTCAGGAAAGTCGTCGCGACCGACAAAATGCTCGTGCCGATTGGTACGTATGGGCCGACCCCA
AGCCCGACGGCACGCCGCCCAACAACTGGCTGTCTATCTTTGGCGGTTCGGCGTGGACATTCGACTCGCGACGTCGTCAG
TATTACCTGCATAATTTCCTGATCGAGCAGCCGGATCTTAATTTTCATAATCCGGCCGTGCGTGACGCTCAGCTCGACAA
CATGCGCTTTTGGCTGGAACTTGGTGTCGATGGCTTTCGCCTGGATACCGTCAACTTCTATTTCCATGATGCCGAACTGC
GCGACAACCCGGCTATCGAGCCCGGCGATACAAAGACGCTGGGTGCTCCGGAGGCCAACCCTTACACGTGGCAACGCCAT
GTCCATGACATAAGCCGCCCCGAAAACCTCGACTTCCTCAAACGATTACGCGCGTTGATGGATGAGTTTCCGGGCACGGC
GACGGTCGGTGAGATCAGCGAAGATAGTCCACTCGAGCGCATGGCGGAGTACACCGCCAATGGTGACAAGCTGCACATGG
CCTACACCTTCGATCTGCTTAATGCACCGCGCTCCCCGGCCTACATTCATGAGGTGATTGAACGCTTCCAGCGACTGGCG
GGCGATGCCTGGCCTTGCTGGGCACTGTCCAACCACGATGTGATGAGAAGCGCCACGCGCTGGGGAGCCGAGGAAGACGC
CGAGGCGTATCCCAAGGTCACGTTGGCCATGCTCTTGTCATTGCGCGGCAGCGTTTGCCTTTATCAAGGTGAGGAACTCG
GTCTGCCCGAGGCCGAAGTGCCTTTCGAGCGTATTCAGGACCCCTACGGCAAGGTCCTTTGGCCTGACTTCAAGGGACGT
GACGGCTGCCGGACGCCGATGCCCTGGCATGACGGTATTCATGGCGGTTTTTCGCGTGTCGAGCCTTGGTTGCCGGTGGA
TGCCCGCCACCTGCAGCGTGCTGTGTCGCGTCAGCAGGCGAGTCTCGATTCCATGCTCAACAGCGTGCGGGCGCTGATCG
CATTTCGTCACGACCACCCGGCGCTGTTCGACGGTGACCTGCGATTGCTCGATGTCGGCGATGCCCTGCTTGGCTTCGTT
CGTGAGCATGCTCAGGAACGCCTTGTGTGCGTGTTCAACCTGACTGACCAGGCGCAGGCGTGTTGCCTGCCCGAGCCAGG
GCACCTGCTAGACGCTCCCGGGTTCACGGCGCACGTGGAAAACGACCGCTTGACCCTGCCGGCCTATCAGGCGGCCTATC
TGCGCCTTGACTGA

Upstream 100 bases:

>100_bases
TGCCGTGTAAACTCCGCGTTTTCGCCTCCTTTTGCCTTGTCTGGTCATCGCTCGCTGCGTTTTCGCACCTGATTCCACTC
TCTTTATCAAGGAATCCAAG

Downstream 100 bases:

>100_bases
ATTTCTTTGGACGATACATGCCGCTGGCGCCTTGTTGGCGCCGGCCAGGAGAACTTTCCATGGCAAGCCTTACGCTCAAC
AAGCTCAACAAGTCGTTTGG

Product: alpha amylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 537; Mature: 537

Protein sequence:

>537_residues
MKDNTFWWRGGVIYQIYPRSFLDSNGDGIGDLPGIIEKLDYVASLGVDGIWLSPFFTSPMADFGYDISDYRDVDPMFGTL
DDFKSLLSHAHALGLKVIIDQVISHTSDQHPWFQESRRDRQNARADWYVWADPKPDGTPPNNWLSIFGGSAWTFDSRRRQ
YYLHNFLIEQPDLNFHNPAVRDAQLDNMRFWLELGVDGFRLDTVNFYFHDAELRDNPAIEPGDTKTLGAPEANPYTWQRH
VHDISRPENLDFLKRLRALMDEFPGTATVGEISEDSPLERMAEYTANGDKLHMAYTFDLLNAPRSPAYIHEVIERFQRLA
GDAWPCWALSNHDVMRSATRWGAEEDAEAYPKVTLAMLLSLRGSVCLYQGEELGLPEAEVPFERIQDPYGKVLWPDFKGR
DGCRTPMPWHDGIHGGFSRVEPWLPVDARHLQRAVSRQQASLDSMLNSVRALIAFRHDHPALFDGDLRLLDVGDALLGFV
REHAQERLVCVFNLTDQAQACCLPEPGHLLDAPGFTAHVENDRLTLPAYQAAYLRLD

Sequences:

>Translated_537_residues
MKDNTFWWRGGVIYQIYPRSFLDSNGDGIGDLPGIIEKLDYVASLGVDGIWLSPFFTSPMADFGYDISDYRDVDPMFGTL
DDFKSLLSHAHALGLKVIIDQVISHTSDQHPWFQESRRDRQNARADWYVWADPKPDGTPPNNWLSIFGGSAWTFDSRRRQ
YYLHNFLIEQPDLNFHNPAVRDAQLDNMRFWLELGVDGFRLDTVNFYFHDAELRDNPAIEPGDTKTLGAPEANPYTWQRH
VHDISRPENLDFLKRLRALMDEFPGTATVGEISEDSPLERMAEYTANGDKLHMAYTFDLLNAPRSPAYIHEVIERFQRLA
GDAWPCWALSNHDVMRSATRWGAEEDAEAYPKVTLAMLLSLRGSVCLYQGEELGLPEAEVPFERIQDPYGKVLWPDFKGR
DGCRTPMPWHDGIHGGFSRVEPWLPVDARHLQRAVSRQQASLDSMLNSVRALIAFRHDHPALFDGDLRLLDVGDALLGFV
REHAQERLVCVFNLTDQAQACCLPEPGHLLDAPGFTAHVENDRLTLPAYQAAYLRLD
>Mature_537_residues
MKDNTFWWRGGVIYQIYPRSFLDSNGDGIGDLPGIIEKLDYVASLGVDGIWLSPFFTSPMADFGYDISDYRDVDPMFGTL
DDFKSLLSHAHALGLKVIIDQVISHTSDQHPWFQESRRDRQNARADWYVWADPKPDGTPPNNWLSIFGGSAWTFDSRRRQ
YYLHNFLIEQPDLNFHNPAVRDAQLDNMRFWLELGVDGFRLDTVNFYFHDAELRDNPAIEPGDTKTLGAPEANPYTWQRH
VHDISRPENLDFLKRLRALMDEFPGTATVGEISEDSPLERMAEYTANGDKLHMAYTFDLLNAPRSPAYIHEVIERFQRLA
GDAWPCWALSNHDVMRSATRWGAEEDAEAYPKVTLAMLLSLRGSVCLYQGEELGLPEAEVPFERIQDPYGKVLWPDFKGR
DGCRTPMPWHDGIHGGFSRVEPWLPVDARHLQRAVSRQQASLDSMLNSVRALIAFRHDHPALFDGDLRLLDVGDALLGFV
REHAQERLVCVFNLTDQAQACCLPEPGHLLDAPGFTAHVENDRLTLPAYQAAYLRLD

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI187423904, Length=453, Percent_Identity=34.2163355408389, Blast_Score=263, Evalue=4e-70,
Organism=Escherichia coli, GI1790687, Length=528, Percent_Identity=36.5530303030303, Blast_Score=288, Evalue=6e-79,
Organism=Escherichia coli, GI1786604, Length=518, Percent_Identity=24.5173745173745, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32565753, Length=383, Percent_Identity=27.6762402088773, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25147709, Length=501, Percent_Identity=23.5528942115768, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6322245, Length=560, Percent_Identity=32.6785714285714, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6321731, Length=557, Percent_Identity=33.9317773788151, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6319776, Length=557, Percent_Identity=33.9317773788151, Blast_Score=277, Evalue=4e-75,
Organism=Saccharomyces cerevisiae, GI6321726, Length=548, Percent_Identity=32.4817518248175, Blast_Score=269, Evalue=7e-73,
Organism=Saccharomyces cerevisiae, GI6324416, Length=554, Percent_Identity=32.8519855595668, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322241, Length=549, Percent_Identity=32.9690346083789, Blast_Score=267, Evalue=3e-72,
Organism=Saccharomyces cerevisiae, GI6322021, Length=549, Percent_Identity=32.9690346083789, Blast_Score=267, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24583747, Length=523, Percent_Identity=37.4760994263862, Blast_Score=308, Evalue=8e-84,
Organism=Drosophila melanogaster, GI24583749, Length=523, Percent_Identity=37.2848948374761, Blast_Score=308, Evalue=9e-84,
Organism=Drosophila melanogaster, GI24586589, Length=516, Percent_Identity=37.015503875969, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24586591, Length=520, Percent_Identity=35.9615384615385, Blast_Score=303, Evalue=2e-82,
Organism=Drosophila melanogaster, GI24586597, Length=535, Percent_Identity=35.8878504672897, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI45549022, Length=522, Percent_Identity=34.0996168582375, Blast_Score=302, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24583745, Length=553, Percent_Identity=35.0813743218806, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24586599, Length=520, Percent_Identity=34.2307692307692, Blast_Score=300, Evalue=2e-81,
Organism=Drosophila melanogaster, GI24586587, Length=562, Percent_Identity=33.4519572953737, Blast_Score=298, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24586593, Length=517, Percent_Identity=34.6228239845261, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI221330053, Length=520, Percent_Identity=32.8846153846154, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI281360393, Length=464, Percent_Identity=31.25, Blast_Score=211, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.20 [H]

Molecular weight: Translated: 61246; Mature: 61246

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNTFWWRGGVIYQIYPRSFLDSNGDGIGDLPGIIEKLDYVASLGVDGIWLSPFFTSPM
CCCCCEEEECCEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHCCCH
ADFGYDISDYRDVDPMFGTLDDFKSLLSHAHALGLKVIIDQVISHTSDQHPWFQESRRDR
HHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QNARADWYVWADPKPDGTPPNNWLSIFGGSAWTFDSRRRQYYLHNFLIEQPDLNFHNPAV
HCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCC
RDAQLDNMRFWLELGVDGFRLDTVNFYFHDAELRDNPAIEPGDTKTLGAPEANPYTWQRH
CCCCCCCEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VHDISRPENLDFLKRLRALMDEFPGTATVGEISEDSPLERMAEYTANGDKLHMAYTFDLL
HHHCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEHH
NAPRSPAYIHEVIERFQRLAGDAWPCWALSNHDVMRSATRWGAEEDAEAYPKVTLAMLLS
CCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LRGSVCLYQGEELGLPEAEVPFERIQDPYGKVLWPDFKGRDGCRTPMPWHDGIHGGFSRV
HCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHC
EPWLPVDARHLQRAVSRQQASLDSMLNSVRALIAFRHDHPALFDGDLRLLDVGDALLGFV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEHHHHHHHHH
REHAQERLVCVFNLTDQAQACCLPEPGHLLDAPGFTAHVENDRLTLPAYQAAYLRLD
HHHHHCCEEEEEECCCCCCCCCCCCCCCEECCCCCEEEECCCEEECCCHHEEEEECC
>Mature Secondary Structure
MKDNTFWWRGGVIYQIYPRSFLDSNGDGIGDLPGIIEKLDYVASLGVDGIWLSPFFTSPM
CCCCCEEEECCEEEEECCHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCHHCCCH
ADFGYDISDYRDVDPMFGTLDDFKSLLSHAHALGLKVIIDQVISHTSDQHPWFQESRRDR
HHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QNARADWYVWADPKPDGTPPNNWLSIFGGSAWTFDSRRRQYYLHNFLIEQPDLNFHNPAV
HCCCCCEEEECCCCCCCCCCCCCEEEECCCEEEEHHHHHHHHHHHHHCCCCCCCCCCCCC
RDAQLDNMRFWLELGVDGFRLDTVNFYFHDAELRDNPAIEPGDTKTLGAPEANPYTWQRH
CCCCCCCEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
VHDISRPENLDFLKRLRALMDEFPGTATVGEISEDSPLERMAEYTANGDKLHMAYTFDLL
HHHCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHCCCCCEEEEEEEEEHH
NAPRSPAYIHEVIERFQRLAGDAWPCWALSNHDVMRSATRWGAEEDAEAYPKVTLAMLLS
CCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHH
LRGSVCLYQGEELGLPEAEVPFERIQDPYGKVLWPDFKGRDGCRTPMPWHDGIHGGFSRV
HCCCEEEEECCCCCCCCCCCCHHHHCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHC
EPWLPVDARHLQRAVSRQQASLDSMLNSVRALIAFRHDHPALFDGDLRLLDVGDALLGFV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEEEHHHHHHHHH
REHAQERLVCVFNLTDQAQACCLPEPGHLLDAPGFTAHVENDRLTLPAYQAAYLRLD
HHHHHCCEEEEEECCCCCCCCCCCCCCCEECCCCCEEEECCCEEECCCHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10400573; 11481430 [H]