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The map label for this gene is mnmG [H]

Identifier: 91785745

GI number: 91785745

Start: 4815874

End: 4817832

Strand: Reverse

Name: mnmG [H]

Synonym: Bxe_A0028

Alternate gene names: 91785745

Gene position: 4817832-4815874 (Counterclockwise)

Preceding gene: 91785746

Following gene: 91785744

Centisome position: 98.41

GC content: 61.15

Gene sequence:

>1959_bases
ATGCTCTTTCCCACAGAATTTGACGTAATCGTCGTCGGCGGCGGTCATGCCGGCACGGAAGCCGCGTTGGCTTCAGCTCG
CATGGGCAATACGACGCTCCTGCTGACCCACAACATCGAAACCCTCGGCCAGATGAGCTGCAATCCGTCGATTGGCGGCA
TTGGCAAAGGCCATCTGGTCAAGGAAGTCGACGCGCTCGGCGGCGCAATGGCGGCCGCGACGGACGAGGGCGGCATCCAG
TTCCGCATCCTGAATTCGTCGAAAGGCCCGGCTGTACGTGCAACGCGCGCTCAGGCTGACCGTCTGCTGTACAAGCAGGC
TATCCGGCACCGGCTCGAAAATCAGCCAAACCTGTGGCTGTTCCAGCAAGCGGTTGACGATCTGATGGTGGAGGGCGACC
GCGTCGTCGGCGCTGTGACGCAGGTGGGAATTCGTTTCCGCGGGCGCGCCGTGGTTTTGACGGCAGGCACGTTCCTCGAC
GGCAAAATTCACGTGGGGTTGAACAACTATACCGGCGGCCGCGCCGGTGATCCGGCGGCGGTTTCGTTGTCGGCGCGTCT
GAAGGAATTGAAGTTGCCGCAAGGCCGTCTCAAGACGGGGACGCCGCCGCGTATCGATGGCCGCACGATCGATTTTTCGC
AACTCGAAGAGCAGCCGGGCGATCTGGATCCGGTGCCGGTGTTCTCGTTCCTCGGGCGCGTCGAGCAGCATCCGCGGCAG
GTGCCGTGCTGGGTTACGCACACGAACGCGCGGACGCACGACATCATCCGTGGCGGACTGGACCGTTCGCCTATGTACAC
CGGGGTGATCGAGGGGGTGGGGCCACGCTATTGCCCATCGATCGAGGACAAGATTCACCGATTCGCTTCAAAGGAGTCAC
ATCAGATCTTCCTCGAGCCAGAAGGGCTGACCACGAACGAGTTCTATCCGAATGGAATCTCCACGAGTCTGCCGTTCGAC
GTTCAACTGGAACTGGTCCGCTCGATGCGAGGTTTGGAGCATGCGCACATCCTGAGACCGGGCTATGCGATCGAATACGA
CTACTTCGATCCGCGTGGGTTGAAGGCTTCGCTCGAAACCAAGGTTATCAGTGGGCTCTTTTTTGCCGGCCAGATCAACG
GCACGACCGGCTATGAAGAGGCAGCAGCGCAGGGTCTGCTGGCCGGCATCAACGCGGGCCTGTATGTGCAGGGCAAGGAA
GCATGGTGCCCGCGCCGCGATCAGGCTTACCTTGGCGTCTTGGTCGACGATTTGGTGACGCGCGGCGTGTCTGAACCGTA
TCGGATGTTCACGAGCCGTGCCGAATATCGTCTGAGTCTGCGCGAAGACAATGCGGATATGCGTCTCACCGAGATTGGCC
GTGAGTTGGGTGTGGTCGACGACGTTCGCTGGGATGCCTTCAGCCGTAAGCGCGACGCTGTTTCACGTGAAACAGAGCGG
CTTCGCACGACATGGGTCAATCCTAAGACGTTATCCGCCGACGAGGCCACAGCGCTGCTCGGCAAGCCAATCGATCACGA
ATATAGTCTGGCGGATCTGCTGCGCCGCCCGGGTGTGTCGTACGACGGCGTTTGCGCGTTGCGTGCCGGCGCGTGCGCCG
CGCCTGAAACCTTGGCCGAAGACGACGTGCTGCTCGCGCAGATCAAGGAACAGATCGAGATTGGCATCAAATATCAGGGC
TACATCGACCGCCAGGCGGGTGAAATCGAGCGAAATGAGGCGCACGAAAGTACGCGTCTGCCCGAAGGGCTGGATTACGC
GGAAGTCAGAGGGCTCTCGTTCGAAGCACGGCAAAAATTGACGCAGTTTCGCCCCGAAACCATCGGCCAGGCGTCGCGTA
TTTCGGGTATTACGCCGGCGGCCATTTCCTTGCTGATGGTCCATCTGAAGCGCGGCTTGGGTCGTCGCCCGACGAAGCCC
GCCGAGTCGGGCACCGACAGCGCGCCGGTGACGCAATGA

Upstream 100 bases:

>100_bases
TGGCCATTCGGACGAAATTCGGGCCTAAACCGAACCCGTTATAATTCGCCCATACATTTCCTTTGAAAGGCTCACGCGAT
GACCTGGCGTGAGATCCGCG

Downstream 100 bases:

>100_bases
CGGTAAGCCAGAAGGGAAGCGGCAGGGAGGCGTTGGAGCCACTGCTGGCGGACGGTGTTCGCGAACTCGGCCTCGCCCTG
AGCGATGCGCAACTCGGCAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 652; Mature: 652

Protein sequence:

>652_residues
MLFPTEFDVIVVGGGHAGTEAALASARMGNTTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDALGGAMAAATDEGGIQ
FRILNSSKGPAVRATRAQADRLLYKQAIRHRLENQPNLWLFQQAVDDLMVEGDRVVGAVTQVGIRFRGRAVVLTAGTFLD
GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSQLEEQPGDLDPVPVFSFLGRVEQHPRQ
VPCWVTHTNARTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEPEGLTTNEFYPNGISTSLPFD
VQLELVRSMRGLEHAHILRPGYAIEYDYFDPRGLKASLETKVISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQGKE
AWCPRRDQAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGVVDDVRWDAFSRKRDAVSRETER
LRTTWVNPKTLSADEATALLGKPIDHEYSLADLLRRPGVSYDGVCALRAGACAAPETLAEDDVLLAQIKEQIEIGIKYQG
YIDRQAGEIERNEAHESTRLPEGLDYAEVRGLSFEARQKLTQFRPETIGQASRISGITPAAISLLMVHLKRGLGRRPTKP
AESGTDSAPVTQ

Sequences:

>Translated_652_residues
MLFPTEFDVIVVGGGHAGTEAALASARMGNTTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDALGGAMAAATDEGGIQ
FRILNSSKGPAVRATRAQADRLLYKQAIRHRLENQPNLWLFQQAVDDLMVEGDRVVGAVTQVGIRFRGRAVVLTAGTFLD
GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSQLEEQPGDLDPVPVFSFLGRVEQHPRQ
VPCWVTHTNARTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEPEGLTTNEFYPNGISTSLPFD
VQLELVRSMRGLEHAHILRPGYAIEYDYFDPRGLKASLETKVISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQGKE
AWCPRRDQAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGVVDDVRWDAFSRKRDAVSRETER
LRTTWVNPKTLSADEATALLGKPIDHEYSLADLLRRPGVSYDGVCALRAGACAAPETLAEDDVLLAQIKEQIEIGIKYQG
YIDRQAGEIERNEAHESTRLPEGLDYAEVRGLSFEARQKLTQFRPETIGQASRISGITPAAISLLMVHLKRGLGRRPTKP
AESGTDSAPVTQ
>Mature_652_residues
MLFPTEFDVIVVGGGHAGTEAALASARMGNTTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDALGGAMAAATDEGGIQ
FRILNSSKGPAVRATRAQADRLLYKQAIRHRLENQPNLWLFQQAVDDLMVEGDRVVGAVTQVGIRFRGRAVVLTAGTFLD
GKIHVGLNNYTGGRAGDPAAVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSQLEEQPGDLDPVPVFSFLGRVEQHPRQ
VPCWVTHTNARTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEPEGLTTNEFYPNGISTSLPFD
VQLELVRSMRGLEHAHILRPGYAIEYDYFDPRGLKASLETKVISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQGKE
AWCPRRDQAYLGVLVDDLVTRGVSEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGVVDDVRWDAFSRKRDAVSRETER
LRTTWVNPKTLSADEATALLGKPIDHEYSLADLLRRPGVSYDGVCALRAGACAAPETLAEDDVLLAQIKEQIEIGIKYQG
YIDRQAGEIERNEAHESTRLPEGLDYAEVRGLSFEARQKLTQFRPETIGQASRISGITPAAISLLMVHLKRGLGRRPTKP
AESGTDSAPVTQ

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=658, Percent_Identity=44.6808510638298, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI19882217, Length=683, Percent_Identity=43.0453879941435, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI183227703, Length=698, Percent_Identity=42.1203438395415, Blast_Score=468, Evalue=1e-131,
Organism=Escherichia coli, GI2367273, Length=637, Percent_Identity=68.1318681318681, Blast_Score=886, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=642, Percent_Identity=40.6542056074766, Blast_Score=470, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321202, Length=633, Percent_Identity=45.1816745655608, Blast_Score=501, Evalue=1e-142,
Organism=Drosophila melanogaster, GI24658174, Length=640, Percent_Identity=46.09375, Blast_Score=495, Evalue=1e-140,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 71430; Mature: 71430

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFPTEFDVIVVGGGHAGTEAALASARMGNTTLLLTHNIETLGQMSCNPSIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHH
KEVDALGGAMAAATDEGGIQFRILNSSKGPAVRATRAQADRLLYKQAIRHRLENQPNLWL
HHHHHHCCHHEECCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQAVDDLMVEGDRVVGAVTQVGIRFRGRAVVLTAGTFLDGKIHVGLNNYTGGRAGDPAA
HHHHHHHHHHCCCHHHHHHHHHCEEECCCEEEEEECEEECCEEEEEECCCCCCCCCCCCE
VSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSQLEEQPGDLDPVPVFSFLGRVEQHPRQ
EEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCC
VPCWVTHTNARTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEP
CCEEEEECCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECC
EGLTTNEFYPNGISTSLPFDVQLELVRSMRGLEHAHILRPGYAIEYDYFDPRGLKASLET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEECCCEEEEECCCCCCCCCHHHHH
KVISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQGKEAWCPRRDQAYLGVLVDDLVT
HHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
RGVSEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGVVDDVRWDAFSRKRDAVSRETER
HCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LRTTWVNPKTLSADEATALLGKPIDHEYSLADLLRRPGVSYDGVCALRAGACAAPETLAE
HHHEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHCC
DDVLLAQIKEQIEIGIKYQGYIDRQAGEIERNEAHESTRLPEGLDYAEVRGLSFEARQKL
CHHHHHHHHHHHHCCEEEECEECCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHH
TQFRPETIGQASRISGITPAAISLLMVHLKRGLGRRPTKPAESGTDSAPVTQ
HHCCCHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLFPTEFDVIVVGGGHAGTEAALASARMGNTTLLLTHNIETLGQMSCNPSIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEEEEECHHHHHCCCCCCCCCCCCCCHHH
KEVDALGGAMAAATDEGGIQFRILNSSKGPAVRATRAQADRLLYKQAIRHRLENQPNLWL
HHHHHHCCHHEECCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEE
FQQAVDDLMVEGDRVVGAVTQVGIRFRGRAVVLTAGTFLDGKIHVGLNNYTGGRAGDPAA
HHHHHHHHHHCCCHHHHHHHHHCEEECCCEEEEEECEEECCEEEEEECCCCCCCCCCCCE
VSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSQLEEQPGDLDPVPVFSFLGRVEQHPRQ
EEHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHCCCC
VPCWVTHTNARTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKESHQIFLEP
CCEEEEECCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECC
EGLTTNEFYPNGISTSLPFDVQLELVRSMRGLEHAHILRPGYAIEYDYFDPRGLKASLET
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHEEECCCEEEEECCCCCCCCCHHHHH
KVISGLFFAGQINGTTGYEEAAAQGLLAGINAGLYVQGKEAWCPRRDQAYLGVLVDDLVT
HHHHHEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHH
RGVSEPYRMFTSRAEYRLSLREDNADMRLTEIGRELGVVDDVRWDAFSRKRDAVSRETER
HCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LRTTWVNPKTLSADEATALLGKPIDHEYSLADLLRRPGVSYDGVCALRAGACAAPETLAE
HHHEECCCCCCCCCHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHCC
DDVLLAQIKEQIEIGIKYQGYIDRQAGEIERNEAHESTRLPEGLDYAEVRGLSFEARQKL
CHHHHHHHHHHHHCCEEEECEECCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHH
TQFRPETIGQASRISGITPAAISLLMVHLKRGLGRRPTKPAESGTDSAPVTQ
HHCCCHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA