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The map label for this gene is rep [H]

Identifier: 91785713

GI number: 91785713

Start: 4774793

End: 4776883

Strand: Reverse

Name: rep [H]

Synonym: Bxe_A0060

Alternate gene names: 91785713

Gene position: 4776883-4774793 (Counterclockwise)

Preceding gene: 91785716

Following gene: 91785710

Centisome position: 97.57

GC content: 62.98

Gene sequence:

>2091_bases
ATGTCCGCAGGCCTGAACCCCGCTCAAAATGAAGCGGTCCGTTATCTCGACGGTCCGTGTCTCGTGCTCGCCGGCGCAGG
CAGCGGCAAGACGCGCGTGATCACGCAGAAAATCGCGCACCTGATCGAGGCCAAGGGCTTCGAGCCGCGCCACATCGCCG
CCGTCACGTTCACGAACAAGGCCGCGCTGGAAATGCGCGAGCGCGTGGGCAAGCTGCTGGAAGGCAAGACGCTCACCACG
CCAGGCAAGGAAGGCCGCAAAGTGCCCGTCAACCAGTTGACGGTGTGCACCTTCCACTCGCTCGGCGTGCAGATTCTGCG
GCAGGAGGCGGAACACGTCGGCCTGAAGCCGCAGTTCTCGATCATGGATTCGGACGACTGCTTCGGCATGATCCAGGAGC
AGGTCGGCTCGACGGACAAGGGTTTCATCCGCAAGATCCAGTCGATCATCTCGCTGTGGAAGAATGGCATGATCATGCCC
GAGCAGGCGATTGCGATTGCGGCGAACGAGGACGAGCATCAGGCCGCGATCGTCTACCGCAATTACGTGGCCACGCTGCA
CGCGTACCAGGCGGTGGATTTCGACGATCTGATCCGCCTGCCCGCCGAGCTCTTCGCGAAGAACGAGCAGGTGCGCGACC
GCTGGCAGAACAAGCTGCGCTATCTGCTGATCGACGAGTATCAGGACACCAACGCCTGCCAGTACGAACTGGTGAAACTG
CTGGCCGGCAAGCGCGCTGCGTTCACGGCGGTGGGCGACGACGATCAGGCCATTTACGGCTGGCGCGGCGCGACGCTCGA
AAATCTCGGGCAGCTCAGCAAGGACTTTCCGAAGCTGCATCTGATCAAGCTGGAGCAGAACTACCGCTCGACGGTGCGCA
TTCTGACCGCCGCGAACAATGTGATCGCGAACAATCCGAAGCTGTTCGAAAAGAAGCTGTGGTCCGAACACGGCATGGGC
GACACGATCACCGTCACGCCCTGCAACGACGAAGAACACGAGGCGGAGTCCGTGGTGTTTCGTCTGTCGGCGCACAAGTT
CGAGCGGCGCGCGAATTTCCGCGACTACGCGATCCTCTATCGCGGCAATTTCCAGGCGCGCATCTTCGAACAGGTGCTGC
GGCGTGAGCGGATTCCGTATGTGCTGTCCGGCGGCCAGTCGTTCTTCGACAAGGCCGAAATCAAGGACATCTGCGCCTAT
CTGCGCCTGATCGCCAACGCGAACGACGACCCCGCGTTCATCCGCGCGATCACCACGCCGCGCCGCGGCGTCGGCAATAC
GACGCTCGAGGCGCTCGGCTCGTTCGCGGGTCAGGCCAAGGTGTCGCTGTTCGAAGCCGTGTATATGGGCGGCATCGAGG
CGCGCCTGTCGCCGCGGCAGATCGAACCCATGCGCGTGTTCTGCGACTTCATGCAGCGCCTCACCGATCGCGCCGAGAAA
GACGCCGCCGGCACGCTGCTCGACGAACTGATGGACGCCATCCACTACGAAGCCTACCTGTACGACGCGTTCGACGAACG
TCAGGCGCAGTCCAAGTGGCAGAACGTGCTGGAGTTCATGGAGTGGTTGAAACGCAAAGGCACCAAGGCTGAGCCGACGG
GCGCCGCGGCGAACGAAGCCACCGGCTACGACACCGCCGATGGTCTCGGCGACACCGGCAAAAGTCTGCTGGGTCTGATC
CAGACCGTCGCGCTGATGTCGATGCTCGAAGGCCGCGAGGAAGATCCCGACGCGGTGCGGCTCTCGACGGTGCACGCGTC
GAAGGGGCTCGAGTATCCGCACGTGTTTCTGGTCGGAGTGGAAGAAGGCATCATGCCGCATCGCGGCGGTCCCGACGACG
AGCCGATCGACGACGCGCGCATCGAAGAAGAGCGCCGGCTCATGTATGTGGCGATCACGCGTGCCCAGCGCAGTTTGCAT
CTGAACTGGTGCAAGAAGCGCAAACGCGCGCGCGAAACGGTGGTGTGCGAGCCGTCGCGCTTCATTCCCGAAATGCTGCT
CGACGACGCTCCGCCGCCGACGCCTGAAGAAGCGCCGATGTCGCCGAAAGACCGGCTCGCGAGTTTGAAGGCGCTGTTGC
AGAAGCCTTGA

Upstream 100 bases:

>100_bases
GGCAGCCGGCGCCGCGCAGCATGCCCCCGGGCCTTCGGCCGGCGTTTCGCGCCGCCGCGCCCTCAACCGCCGCTTTAACC
GCATCACCCATCCACAGACG

Downstream 100 bases:

>100_bases
GCGGTGAAGCCGCTGATCTGGCTATAGCGCCCATCTACGGTCGATAAAAAAATCCCTGCTCCGCGTTTCCGCGGGCAGGG
ATTTTTTGCGACGTGGCTCA

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAALEMRERVGKLLEGKTLTT
PGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQVGSTDKGFIRKIQSIISLWKNGMIMP
EQAIAIAANEDEHQAAIVYRNYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKL
LAGKRAAFTAVGDDDQAIYGWRGATLENLGQLSKDFPKLHLIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMG
DTITVTPCNDEEHEAESVVFRLSAHKFERRANFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDICAY
LRLIANANDDPAFIRAITTPRRGVGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSPRQIEPMRVFCDFMQRLTDRAEK
DAAGTLLDELMDAIHYEAYLYDAFDERQAQSKWQNVLEFMEWLKRKGTKAEPTGAAANEATGYDTADGLGDTGKSLLGLI
QTVALMSMLEGREEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGPDDEPIDDARIEEERRLMYVAITRAQRSLH
LNWCKKRKRARETVVCEPSRFIPEMLLDDAPPPTPEEAPMSPKDRLASLKALLQKP

Sequences:

>Translated_696_residues
MSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAALEMRERVGKLLEGKTLTT
PGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQVGSTDKGFIRKIQSIISLWKNGMIMP
EQAIAIAANEDEHQAAIVYRNYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKL
LAGKRAAFTAVGDDDQAIYGWRGATLENLGQLSKDFPKLHLIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMG
DTITVTPCNDEEHEAESVVFRLSAHKFERRANFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDICAY
LRLIANANDDPAFIRAITTPRRGVGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSPRQIEPMRVFCDFMQRLTDRAEK
DAAGTLLDELMDAIHYEAYLYDAFDERQAQSKWQNVLEFMEWLKRKGTKAEPTGAAANEATGYDTADGLGDTGKSLLGLI
QTVALMSMLEGREEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGPDDEPIDDARIEEERRLMYVAITRAQRSLH
LNWCKKRKRARETVVCEPSRFIPEMLLDDAPPPTPEEAPMSPKDRLASLKALLQKP
>Mature_695_residues
SAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAALEMRERVGKLLEGKTLTTP
GKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQVGSTDKGFIRKIQSIISLWKNGMIMPE
QAIAIAANEDEHQAAIVYRNYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKLL
AGKRAAFTAVGDDDQAIYGWRGATLENLGQLSKDFPKLHLIKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGD
TITVTPCNDEEHEAESVVFRLSAHKFERRANFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDICAYL
RLIANANDDPAFIRAITTPRRGVGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSPRQIEPMRVFCDFMQRLTDRAEKD
AAGTLLDELMDAIHYEAYLYDAFDERQAQSKWQNVLEFMEWLKRKGTKAEPTGAAANEATGYDTADGLGDTGKSLLGLIQ
TVALMSMLEGREEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGPDDEPIDDARIEEERRLMYVAITRAQRSLHL
NWCKKRKRARETVVCEPSRFIPEMLLDDAPPPTPEEAPMSPKDRLASLKALLQKP

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=701, Percent_Identity=46.2196861626248, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI2367296, Length=665, Percent_Identity=35.3383458646617, Blast_Score=352, Evalue=4e-98,
Organism=Saccharomyces cerevisiae, GI6322369, Length=723, Percent_Identity=28.0774550484094, Blast_Score=202, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6324477, Length=659, Percent_Identity=23.3687405159332, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78192; Mature: 78061

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNK
CCCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
AALEMRERVGKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFS
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQVGSTDKGFIRKIQSIISLWKNGMIMPEQAIAIAANEDEHQAAIVYR
ECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEHHHH
NYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKL
HHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
LAGKRAAFTAVGDDDQAIYGWRGATLENLGQLSKDFPKLHLIKLEQNYRSTVRILTAANN
HHCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHEEEHHHCCC
VIANNPKLFEKKLWSEHGMGDTITVTPCNDEEHEAESVVFRLSAHKFERRANFRDYAILY
HHCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
RGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANANDDPAFIRAITTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
RRGVGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSPRQIEPMRVFCDFMQRLTDRAEK
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
DAAGTLLDELMDAIHYEAYLYDAFDERQAQSKWQNVLEFMEWLKRKGTKAEPTGAAANEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TGYDTADGLGDTGKSLLGLIQTVALMSMLEGREEDPDAVRLSTVHASKGLEYPHVFLVGV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEE
EEGIMPHRGGPDDEPIDDARIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSR
CCCCCCCCCCCCCCCCCCHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCHH
FIPEMLLDDAPPPTPEEAPMSPKDRLASLKALLQKP
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNK
CCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
AALEMRERVGKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFS
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQVGSTDKGFIRKIQSIISLWKNGMIMPEQAIAIAANEDEHQAAIVYR
ECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCEEHHHH
NYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELVKL
HHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
LAGKRAAFTAVGDDDQAIYGWRGATLENLGQLSKDFPKLHLIKLEQNYRSTVRILTAANN
HHCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCEEEEEECCCHHHHEEEHHHCCC
VIANNPKLFEKKLWSEHGMGDTITVTPCNDEEHEAESVVFRLSAHKFERRANFRDYAILY
HHCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
RGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANANDDPAFIRAITTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
RRGVGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSPRQIEPMRVFCDFMQRLTDRAEK
CCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHH
DAAGTLLDELMDAIHYEAYLYDAFDERQAQSKWQNVLEFMEWLKRKGTKAEPTGAAANEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TGYDTADGLGDTGKSLLGLIQTVALMSMLEGREEDPDAVRLSTVHASKGLEYPHVFLVGV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEE
EEGIMPHRGGPDDEPIDDARIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSR
CCCCCCCCCCCCCCCCCCHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCHH
FIPEMLLDDAPPPTPEEAPMSPKDRLASLKALLQKP
HHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]