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The map label for this gene is metF [H]

Identifier: 91785597

GI number: 91785597

Start: 4654619

End: 4655449

Strand: Reverse

Name: metF [H]

Synonym: Bxe_A0180

Alternate gene names: 91785597

Gene position: 4655449-4654619 (Counterclockwise)

Preceding gene: 91785598

Following gene: 91785596

Centisome position: 95.09

GC content: 62.82

Gene sequence:

>831_bases
ATGAACCCGATCGAACTTTCATTCGAATTCTTCCCGCCGAAAACGCAGGAAGGCGTGGACAAGCTGCGCGCCACGCGCGC
CCAGCTCGCCACGCTCAAGCCCAAGTTCGTGTCCGTCACGTTCGGCGCCGGCGGCTCGACGCAACAGGGCACGCTCGACA
CCGTCGTCGATATGGCGAAGGAAGGGCTCGAAGCGGCGCCGCACGTGTCGTGCATCGGCTCGTCGAAAGAGAGCCTGCGC
GCCATTCTCAACGAGTACCGCGCACATGGCATCCGCCATATCGTCGCGCTGCGCGGCGATCTGCCGTCCGGCATGGGCGA
AGTCGGCGAGCTGCGCTATGCGTCGGAACTGGTGAGCTTTATCCGCGCCGAATTCGGCGACTGGTTCTGCATCGAGGTGG
CCGGCTATCCGGAATACCACCCGCAGTCGCGCTCGCCGCGTCAGGATCTGGAAAACTTCGCCCGCAAGGTGAAGGCCGGC
GCCAATTCGGCGATCACACAGTACTTCTTCAATGCAGACGCGTATTTCCGTTTCGTCGACGACGCGAGAAAGCTCGGCGT
GGACGTGCCGATCGTGCCGGGCATCATGCCGATCACGAACTTCTCGCAGCTGATGCGTTTCTCGGAGATGTGCGGCGCTG
AAGTGCCACGCTGGATCGCGCGCCGGCTGGAAAGCTTCGGCGACGATCGCGAGTCAATTCGCGCGTTCGGGCTGGATGTG
GTGACGGACCTGTGCAGGCGTCTGATCGATGCGAAGGTGCCGGGCCTGCACTTCTACACGCTAAACGGCGCAGCGGCGAC
CAAGGCGATCTGCGAACGGTTGAACGTTTAA

Upstream 100 bases:

>100_bases
GTTCTTCGGCTCGATTCTGTACAGCATCGTTTCGTGGCTGCTGTCCGCGCTTATTTTCGGCAACCGCAGTCTCGGTTGAC
CGACGATTGACTAAAGAATC

Downstream 100 bases:

>100_bases
GCTCGAATCGGTCTGTCGAGACGAAACCGCGCGGCTGAGAAACAGTCGCGCGGTTTTTTTTGCACCGCGAATAGCGTACA
GGCTGCCACGCGGATGCCAC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MNPIELSFEFFPPKTQEGVDKLRATRAQLATLKPKFVSVTFGAGGSTQQGTLDTVVDMAKEGLEAAPHVSCIGSSKESLR
AILNEYRAHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEFGDWFCIEVAGYPEYHPQSRSPRQDLENFARKVKAG
ANSAITQYFFNADAYFRFVDDARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDRESIRAFGLDV
VTDLCRRLIDAKVPGLHFYTLNGAAATKAICERLNV

Sequences:

>Translated_276_residues
MNPIELSFEFFPPKTQEGVDKLRATRAQLATLKPKFVSVTFGAGGSTQQGTLDTVVDMAKEGLEAAPHVSCIGSSKESLR
AILNEYRAHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEFGDWFCIEVAGYPEYHPQSRSPRQDLENFARKVKAG
ANSAITQYFFNADAYFRFVDDARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDRESIRAFGLDV
VTDLCRRLIDAKVPGLHFYTLNGAAATKAICERLNV
>Mature_276_residues
MNPIELSFEFFPPKTQEGVDKLRATRAQLATLKPKFVSVTFGAGGSTQQGTLDTVVDMAKEGLEAAPHVSCIGSSKESLR
AILNEYRAHGIRHIVALRGDLPSGMGEVGELRYASELVSFIRAEFGDWFCIEVAGYPEYHPQSRSPRQDLENFARKVKAG
ANSAITQYFFNADAYFRFVDDARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDRESIRAFGLDV
VTDLCRRLIDAKVPGLHFYTLNGAAATKAICERLNV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=280, Percent_Identity=39.2857142857143, Blast_Score=190, Evalue=1e-48,
Organism=Escherichia coli, GI1790377, Length=274, Percent_Identity=45.6204379562044, Blast_Score=243, Evalue=8e-66,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=32.9787234042553, Blast_Score=171, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=32.9787234042553, Blast_Score=171, Evalue=4e-43,
Organism=Saccharomyces cerevisiae, GI6321313, Length=281, Percent_Identity=35.5871886120996, Blast_Score=177, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6325234, Length=287, Percent_Identity=32.7526132404181, Blast_Score=161, Evalue=9e-41,
Organism=Drosophila melanogaster, GI24662658, Length=224, Percent_Identity=26.7857142857143, Blast_Score=80, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 30602; Mature: 30602

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIELSFEFFPPKTQEGVDKLRATRAQLATLKPKFVSVTFGAGGSTQQGTLDTVVDMAK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
EGLEAAPHVSCIGSSKESLRAILNEYRAHGIRHIVALRGDLPSGMGEVGELRYASELVSF
HCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
IRAEFGDWFCIEVAGYPEYHPQSRSPRQDLENFARKVKAGANSAITQYFFNADAYFRFVD
HHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDRESIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
VTDLCRRLIDAKVPGLHFYTLNGAAATKAICERLNV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MNPIELSFEFFPPKTQEGVDKLRATRAQLATLKPKFVSVTFGAGGSTQQGTLDTVVDMAK
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHH
EGLEAAPHVSCIGSSKESLRAILNEYRAHGIRHIVALRGDLPSGMGEVGELRYASELVSF
HCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHH
IRAEFGDWFCIEVAGYPEYHPQSRSPRQDLENFARKVKAGANSAITQYFFNADAYFRFVD
HHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHH
DARKLGVDVPIVPGIMPITNFSQLMRFSEMCGAEVPRWIARRLESFGDDRESIRAFGLDV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHH
VTDLCRRLIDAKVPGLHFYTLNGAAATKAICERLNV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]