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The map label for this gene is tuf1 [H]

Identifier: 91785467

GI number: 91785467

Start: 4514871

End: 4516061

Strand: Reverse

Name: tuf1 [H]

Synonym: Bxe_A0312

Alternate gene names: 91785467

Gene position: 4516061-4514871 (Counterclockwise)

Preceding gene: 91785468

Following gene: 91785466

Centisome position: 92.24

GC content: 61.29

Gene sequence:

>1191_bases
ATGGCTAAAGGTAAATTCGAACGGACCAAGCCGCACGTGAACGTCGGCACGATCGGTCACGTTGACCACGGCAAGACCAC
GCTGACGGCAGCGATCACGACGGTTCTGACGCAGAAGTTTGGCGGCGAAGCGAAGGCATACGACCAGATCGACGCGGCGC
CGGAAGAGAAGGCGCGTGGTATCACGATCAACACGGCACACGTCGAGTACGAAACGGCTAACCGCCACTACGCACACGTC
GACTGCCCGGGCCACGCTGACTATGTGAAGAACATGATCACGGGCGCAGCGCAGATGGACGGCGCGATCCTGGTGTGCTC
GGCCGCTGACGGCCCGATGCCGCAAACGCGCGAGCACATCCTGCTGGCGCGTCAGGTTGGCGTGCCGTACATCATCGTGT
TCCTGAACAAGTGCGACATGGTGGACGACGCTGAGCTGCTGGAGCTGGTCGAGATGGAAGTTCGCGAACTTCTGTCGAAG
TACGACTTCCCGGGCGACGAGACGCCGATCATCAAGGGTTCGGCGAAGCTGGCGCTGGAAGGCGACAAGGGCGAGCTGGG
CGAAGTGGCGATCATGAATCTGGCCGACGCGCTGGACACGTACATCCCGACGCCGGAGCGCGCAGTTGACGGTGCGTTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCGGGTCGCGGTACGGTGGTGACGGGTCGCGTTGAGCGCGGCGTCGTGAAG
GTCGGCGAGGAAATCGAAATTGTCGGTATCAAGCCGACGGTGAAGACGACCTGCACGGGCGTGGAAATGTTCCGCAAGCT
GCTCGATCAAGGTCAGGCGGGCGACAACGTGGGTATCCTGCTGCGCGGCACGAAGCGTGAAGACGTGGAGCGTGGCCAGG
TGCTGGCCAAGCCGGGTTCGATCAACCCGCACACGCACTTCACGGCTGAAGTGTACGTGCTGAGCAAGGACGAAGGTGGC
CGTCACACGCCGTTCTTCAACAACTATCGTCCGCAGTTCTACTTCCGTACGACGGACGTGACGGGCTCGATCGAGTTGCC
GAAGGACAAGGAAATGGTCATGCCGGGCGACAACGTGTCGATCACGGTGAAGCTGATCAACCCGATCGCGATGGAAGAAG
GTCTGCGTTTCGCAATCCGCGAAGGCGGCCGTACGGTCGGCGCAGGTGTGGTTGCCAAGATTCTCGAGTAA

Upstream 100 bases:

>100_bases
CCGAAGCACCGCGTAACGTGTCCGAAGCGATCATCAACGCGAAGTCGAAGTAATCGCGCGGCAAGTCATTCAACGATTAA
CTTTTTGAAAGAAGAGAAAC

Downstream 100 bases:

>100_bases
AATCGCGGCTTGACGTATTTGCTGCAAGTGGTGCCCGGAGCCGGGCGCCGGCCCTCAGCGGCCGCCCGGCTCTACGTTCT
TTACTGTTTGGCGGTGCAAT

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTQKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDETPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVVK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSINPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKILE

Sequences:

>Translated_396_residues
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTQKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDETPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVVK
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSINPHTHFTAEVYVLSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKILE
>Mature_395_residues
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTQKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKY
DFPGDETPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVVKV
GEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGSINPHTHFTAEVYVLSKDEGGR
HTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVSITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKILE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.6142131979695, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=28.125, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI223555963, Length=431, Percent_Identity=29.6983758700696, Blast_Score=155, Evalue=9e-38,
Organism=Homo sapiens, GI5729864, Length=431, Percent_Identity=29.6983758700696, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=29.6380090497738, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI46094014, Length=439, Percent_Identity=27.1070615034169, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=27.8801843317972, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=27.8801843317972, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=27.8801843317972, Blast_Score=118, Evalue=8e-27,
Organism=Homo sapiens, GI54607086, Length=330, Percent_Identity=28.7878787878788, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=28.7313432835821, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=31.1111111111111, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI157426893, Length=285, Percent_Identity=27.0175438596491, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=80.5063291139241, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=80.5063291139241, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=338, Percent_Identity=28.9940828402367, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI48994988, Length=277, Percent_Identity=30.6859205776173, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789108, Length=316, Percent_Identity=27.8481012658228, Blast_Score=82, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17556456, Length=403, Percent_Identity=53.1017369727047, Blast_Score=412, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=44.702842377261, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17552884, Length=449, Percent_Identity=30.0668151447661, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17569207, Length=449, Percent_Identity=30.0668151447661, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI32566303, Length=436, Percent_Identity=28.6697247706422, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17506081, Length=275, Percent_Identity=32.3636363636364, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI115532067, Length=431, Percent_Identity=26.9141531322506, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=436, Percent_Identity=26.605504587156, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=352, Percent_Identity=28.9772727272727, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=37.4149659863946, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=295, Percent_Identity=26.7796610169492, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=27.8481012658228, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.1616161616162, Blast_Score=535, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=445, Percent_Identity=30.7865168539326, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=445, Percent_Identity=30.7865168539326, Blast_Score=153, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=29.7619047619048, Blast_Score=94, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6322937, Length=358, Percent_Identity=25.9776536312849, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=28.3018867924528, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323098, Length=192, Percent_Identity=29.6875, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=58.7786259541985, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=58.7786259541985, Blast_Score=488, Evalue=1e-138,
Organism=Drosophila melanogaster, GI19921738, Length=388, Percent_Identity=51.8041237113402, Blast_Score=413, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652838, Length=448, Percent_Identity=29.4642857142857, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17137572, Length=448, Percent_Identity=29.4642857142857, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI45553807, Length=448, Percent_Identity=29.4642857142857, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45553816, Length=448, Percent_Identity=29.4642857142857, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24651721, Length=448, Percent_Identity=29.4642857142857, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17864154, Length=448, Percent_Identity=29.4642857142857, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17137380, Length=337, Percent_Identity=30.8605341246291, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI45550900, Length=436, Percent_Identity=27.9816513761468, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI19922690, Length=307, Percent_Identity=29.9674267100977, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=28.996282527881, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.996282527881, Blast_Score=92, Evalue=9e-19,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43059; Mature: 42928

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTQKFGGEAKAYDQIDAAPEEKARG
CCCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDETPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVVK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCHHCCCEE
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
INPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKILE
EEEEEECCHHHHHCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKGKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLTQKFGGEAKAYDQIDAAPEEKARG
CCCCCCCCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCCCCHHHCCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIIVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDETPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGAFLMPVEDVFSISGRGTVVTGRVERGVVK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEECCHHCCCEE
VGEEIEIVGIKPTVKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLAKPGS
CCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEEEECCCC
INPHTHFTAEVYVLSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPKDKEMVMPGDNVS
CCCCCEEEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCCCCEEECCCCCE
ITVKLINPIAMEEGLRFAIREGGRTVGAGVVAKILE
EEEEEECCHHHHHCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA