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The map label for this gene is araF [H]

Identifier: 91785233

GI number: 91785233

Start: 4280132

End: 4281130

Strand: Reverse

Name: araF [H]

Synonym: Bxe_A0546

Alternate gene names: 91785233

Gene position: 4281130-4280132 (Counterclockwise)

Preceding gene: 91785234

Following gene: 91785232

Centisome position: 87.44

GC content: 64.36

Gene sequence:

>999_bases
ATGAAACGTAGAATTTTCCTCACGCTGGCAGCAGCGGCGGCGGGTGTGCTCTTCAACGCACCGGTCGCGCAGGCCGCTGA
TCCGGTGAAGATCGGTTTCCTCGTGAAGCAGCCGGAAGAGCCGTGGTTCCAGGACGAGTGGAAGTTCGCCGAGATCGCCG
CCAAAGAGAAGGGCTTCACGCTGGTGAAGATCGGCGCGCCGTCGGGCGAGAAGGTGATGAGCGCCATCGACAACCTGGCG
GCGCAGAAGGCGCAAGGCTTCGTGATCTGCACGCCTGACGTCAAGCTCGGACCGGGCATCGTCGCGAAAGCGAAAGCCGA
CGGCCTGAAGATGATGACGGTGGACGACCGTCTGGTCGACGGCGCGGGCAAGCCGATCGCGTCGGTGCCGCATATGGGCA
TCTCGGCGTACAACATCGGCAAGCAGGTCGGCGACGGCCTGGCTGCCGAAATCAAGAAGCGCGGCTGGGACATGAAGGAC
GTGGGCGCGATCGACGTGACCTACGAACAGTTGCCCACCGCGCATGACCGCACCAGCGGCGCAACCGACGCACTGGTCGC
CGCCGGCTTCCCGAAAGCGAACGTGATCGCCGCGCCGCAAGCGAAGACCGACACGGAAAACGCGTTCAACGCGGCCAACA
TCGCGCTGACGAAGAATCCGAACTTCAAGCACTGGGTCGCCTACGGTCTGAACGACGAAGCCGTGCTCGGCGCGGTGCGC
GCAGCGGAAGGGCGCGGCTTCAAGGCGGACAACATGATCGGTATCGGCATCGGCGGTTCGGACTCGGCACTCAACGAGTT
CAAGAAGCCGTCGCCGACGGGCTTCTACGGCACGGTGATCATCAGCCCGAAGCGCCACGGCGAGGAAACCTCCACGCTGA
TGTACGACTGGATCACGCAAGGCAAGGCACCGCCGCCGCTGACCCTGACCACCGGCATGCTGGCCACGCGTGACAACGTC
GCGGATGTGCGCCAGAAGATGGGCCTCGCGGCCAACTAA

Upstream 100 bases:

>100_bases
ACGAGTACACCGGACGACGCGCTGGCCGCGTGTGTCACACCCGGTACAAGAAGACGCGGCCGATACCTTCTCGTTATCAA
TTAGTCAGGAGACACGCATT

Downstream 100 bases:

>100_bases
GCGGTTTGGTTGGGACGCGGGACATGCAGTGAACTGCCGGCGCGTGTTTCTCTTTCGCGGAGAGAGTGCGCGCGCCGGCA
GCAGTATTAAAAGATCAGCA

Product: ABC sugar(arabinose) transporter, periplasmic ligand binding protein

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKRRIFLTLAAAAAGVLFNAPVAQAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKEKGFTLVKIGAPSGEKVMSAIDNLA
AQKAQGFVICTPDVKLGPGIVAKAKADGLKMMTVDDRLVDGAGKPIASVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKD
VGAIDVTYEQLPTAHDRTSGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWVAYGLNDEAVLGAVR
AAEGRGFKADNMIGIGIGGSDSALNEFKKPSPTGFYGTVIISPKRHGEETSTLMYDWITQGKAPPPLTLTTGMLATRDNV
ADVRQKMGLAAN

Sequences:

>Translated_332_residues
MKRRIFLTLAAAAAGVLFNAPVAQAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKEKGFTLVKIGAPSGEKVMSAIDNLA
AQKAQGFVICTPDVKLGPGIVAKAKADGLKMMTVDDRLVDGAGKPIASVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKD
VGAIDVTYEQLPTAHDRTSGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWVAYGLNDEAVLGAVR
AAEGRGFKADNMIGIGIGGSDSALNEFKKPSPTGFYGTVIISPKRHGEETSTLMYDWITQGKAPPPLTLTTGMLATRDNV
ADVRQKMGLAAN
>Mature_332_residues
MKRRIFLTLAAAAAGVLFNAPVAQAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKEKGFTLVKIGAPSGEKVMSAIDNLA
AQKAQGFVICTPDVKLGPGIVAKAKADGLKMMTVDDRLVDGAGKPIASVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKD
VGAIDVTYEQLPTAHDRTSGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWVAYGLNDEAVLGAVR
AAEGRGFKADNMIGIGIGGSDSALNEFKKPSPTGFYGTVIISPKRHGEETSTLMYDWITQGKAPPPLTLTTGMLATRDNV
ADVRQKMGLAAN

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=324, Percent_Identity=48.1481481481481, Blast_Score=309, Evalue=1e-85,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35062; Mature: 35062

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRIFLTLAAAAAGVLFNAPVAQAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKEKGFT
CCEEEEEEEHHHHHHHHCCCCHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCE
LVKIGAPSGEKVMSAIDNLAAQKAQGFVICTPDVKLGPGIVAKAKADGLKMMTVDDRLVD
EEEEECCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEECCCCEEEEEECCHHHC
GAGKPIASVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKDVGAIDVTYEQLPTAHDRTSG
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEHHHCCCCCCCCCC
ATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWVAYGLNDEAVLGAVR
CCHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHH
AAEGRGFKADNMIGIGIGGSDSALNEFKKPSPTGFYGTVIISPKRHGEETSTLMYDWITQ
HCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHEEEEHHHC
GKAPPPLTLTTGMLATRDNVADVRQKMGLAAN
CCCCCCEEEECCCEECCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MKRRIFLTLAAAAAGVLFNAPVAQAADPVKIGFLVKQPEEPWFQDEWKFAEIAAKEKGFT
CCEEEEEEEHHHHHHHHCCCCHHHCCCCEEEEEEEECCCCCCCCCCCHHHHHHHCCCCCE
LVKIGAPSGEKVMSAIDNLAAQKAQGFVICTPDVKLGPGIVAKAKADGLKMMTVDDRLVD
EEEEECCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEECCCCEEEEEECCHHHC
GAGKPIASVPHMGISAYNIGKQVGDGLAAEIKKRGWDMKDVGAIDVTYEQLPTAHDRTSG
CCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEHHHCCCCCCCCCC
ATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPNFKHWVAYGLNDEAVLGAVR
CCHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHHHHH
AAEGRGFKADNMIGIGIGGSDSALNEFKKPSPTGFYGTVIISPKRHGEETSTLMYDWITQ
HCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHEEEEHHHC
GKAPPPLTLTTGMLATRDNVADVRQKMGLAAN
CCCCCCEEEECCCEECCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]