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The map label for this gene is fghA [C]

Identifier: 91785066

GI number: 91785066

Start: 4091121

End: 4091978

Strand: Reverse

Name: fghA [C]

Synonym: Bxe_A0714

Alternate gene names: 91785066

Gene position: 4091978-4091121 (Counterclockwise)

Preceding gene: 91785067

Following gene: 91785061

Centisome position: 83.58

GC content: 66.2

Gene sequence:

>858_bases
ATGGCGATGCTTGAACTTCTGTCGTCGCACGCCTGTCACGGCGGCGAGCAGCGCATCTATCGGCACGACTCGCAGACCAT
CGGCCTGCCGATGCGCTTCTCCATCTATCTACCGCCGCAGGCCTTGCAGGCCAATGCGAACGTGCCCGCGCTGTTCTACC
TCGCGGGTCTCACGTGCACGGAAGAGACGTTTCCGGTCAAAGCCGGCGCGCAGCGCTTCGCGGCGCAGCACGGCATTGCG
CTCATCGCGCCGGACACCAGTCCGCGCGGTGCGGGCGTGCCGGGTGAAAGCGCGGCGTGGGACTTCGGCGTGGGCGCGGG
CTTTTACGTCGACGCCACGCAGCAGCCGTGGGCGCAGCACTATCGCATGTACTCGTACGTGCGTGACGAACTGCGCGAAA
CGGTGCTCGCGAATCTGCCGGTAGACGGCGCGCGTCTCGGCATCTTCGGGCATTCGATGGGCGGTCACGGCGCGTTGATG
CTGGCGCTGCGCAATCCGGAGATCTATCGGTCGGTATCGGCGTTCGCGCCGATCGCCGCGCCGACGCGTTGCCCGTGGGG
TGAGAAAGCGTTCAGCGGTTATCTGGGCGCAGACCGCGAAGCGTGGAAGCAGTACGACGCGAGCGAGCTGGTCGCGCAGG
CGTCGCGCAAGTTTGCGGAGGGGATTCTGGTGGATCAGGGCCTCGCGGATCAGTTCCTCGCGGAGCAACTCAATCCGGAC
GTGTTCGAAGCCGCCTGCCAGGCCGCGGGTCAGCCGCTGACGCTGCGCCGGCACGCGGGGTACGACCACGGCTACTATTT
CATCTCCACGTTCATCGAGGATCATCTCGCGCATCACGCGAAGGTGCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
TGCCGCTCGAACGCATCAACGAAGGCTTCGATCTGATGAAGAAGGGCGAGTCGATCCGCTCGGTCGTGCTGTACTAATCC
AATGCGGCACAACGAGGAGC

Downstream 100 bases:

>100_bases
AGTCTTTCGCAGGGAGAAGGCGGCGTGCGGATGCTCCGCCGCCTTCGTCCTCGTTTTCGTCTTGCTTCAACGCCGTTTCA
ACAAACTCCCGCCGTACACC

Product: S-formylglutathione hydrolase (FghA)

Products: NA

Alternate protein names: FGH [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MAMLELLSSHACHGGEQRIYRHDSQTIGLPMRFSIYLPPQALQANANVPALFYLAGLTCTEETFPVKAGAQRFAAQHGIA
LIAPDTSPRGAGVPGESAAWDFGVGAGFYVDATQQPWAQHYRMYSYVRDELRETVLANLPVDGARLGIFGHSMGGHGALM
LALRNPEIYRSVSAFAPIAAPTRCPWGEKAFSGYLGADREAWKQYDASELVAQASRKFAEGILVDQGLADQFLAEQLNPD
VFEAACQAAGQPLTLRRHAGYDHGYYFISTFIEDHLAHHAKVLLG

Sequences:

>Translated_285_residues
MAMLELLSSHACHGGEQRIYRHDSQTIGLPMRFSIYLPPQALQANANVPALFYLAGLTCTEETFPVKAGAQRFAAQHGIA
LIAPDTSPRGAGVPGESAAWDFGVGAGFYVDATQQPWAQHYRMYSYVRDELRETVLANLPVDGARLGIFGHSMGGHGALM
LALRNPEIYRSVSAFAPIAAPTRCPWGEKAFSGYLGADREAWKQYDASELVAQASRKFAEGILVDQGLADQFLAEQLNPD
VFEAACQAAGQPLTLRRHAGYDHGYYFISTFIEDHLAHHAKVLLG
>Mature_284_residues
AMLELLSSHACHGGEQRIYRHDSQTIGLPMRFSIYLPPQALQANANVPALFYLAGLTCTEETFPVKAGAQRFAAQHGIAL
IAPDTSPRGAGVPGESAAWDFGVGAGFYVDATQQPWAQHYRMYSYVRDELRETVLANLPVDGARLGIFGHSMGGHGALML
ALRNPEIYRSVSAFAPIAAPTRCPWGEKAFSGYLGADREAWKQYDASELVAQASRKFAEGILVDQGLADQFLAEQLNPDV
FEAACQAAGQPLTLRRHAGYDHGYYFISTFIEDHLAHHAKVLLG

Specific function: Serine hydrolase involved in the detoxification of formaldehyde. Hydrolyzes S-formylglutathione to glutathione and formate [H]

COG id: COG0627

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the esterase D family [H]

Homologues:

Organism=Homo sapiens, GI33413400, Length=281, Percent_Identity=55.8718861209964, Blast_Score=335, Evalue=3e-92,
Organism=Escherichia coli, GI1786551, Length=280, Percent_Identity=50.3571428571429, Blast_Score=295, Evalue=2e-81,
Organism=Escherichia coli, GI1788477, Length=281, Percent_Identity=53.3807829181495, Blast_Score=292, Evalue=2e-80,
Organism=Caenorhabditis elegans, GI17510185, Length=280, Percent_Identity=50.3571428571429, Blast_Score=286, Evalue=7e-78,
Organism=Saccharomyces cerevisiae, GI6322393, Length=296, Percent_Identity=38.8513513513513, Blast_Score=216, Evalue=3e-57,
Organism=Drosophila melanogaster, GI45551932, Length=282, Percent_Identity=52.8368794326241, Blast_Score=307, Evalue=6e-84,
Organism=Drosophila melanogaster, GI24648347, Length=282, Percent_Identity=52.8368794326241, Blast_Score=306, Evalue=8e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000801
- InterPro:   IPR014186 [H]

Pfam domain/function: PF00756 Esterase [H]

EC number: =3.1.2.12 [H]

Molecular weight: Translated: 31066; Mature: 30935

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMLELLSSHACHGGEQRIYRHDSQTIGLPMRFSIYLPPQALQANANVPALFYLAGLTCT
CHHHHHHHHHCCCCCHHHHEECCCCEECCCEEEEEEECHHHHHCCCCCCEEEEEECCEEC
EETFPVKAGAQRFAAQHGIALIAPDTSPRGAGVPGESAAWDFGVGAGFYVDATQQPWAQH
CCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEECCCCCCEEEECCCCHHHHH
YRMYSYVRDELRETVLANLPVDGARLGIFGHSMGGHGALMLALRNPEIYRSVSAFAPIAA
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCC
PTRCPWGEKAFSGYLGADREAWKQYDASELVAQASRKFAEGILVDQGLADQFLAEQLNPD
CCCCCCCHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHH
VFEAACQAAGQPLTLRRHAGYDHGYYFISTFIEDHLAHHAKVLLG
HHHHHHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AMLELLSSHACHGGEQRIYRHDSQTIGLPMRFSIYLPPQALQANANVPALFYLAGLTCT
HHHHHHHHHCCCCCHHHHEECCCCEECCCEEEEEEECHHHHHCCCCCCEEEEEECCEEC
EETFPVKAGAQRFAAQHGIALIAPDTSPRGAGVPGESAAWDFGVGAGFYVDATQQPWAQH
CCCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCEECCCCCCEEEECCCCHHHHH
YRMYSYVRDELRETVLANLPVDGARLGIFGHSMGGHGALMLALRNPEIYRSVSAFAPIAA
HHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEEECCCHHHHHHHHHCCCCC
PTRCPWGEKAFSGYLGADREAWKQYDASELVAQASRKFAEGILVDQGLADQFLAEQLNPD
CCCCCCCHHHHHCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHH
VFEAACQAAGQPLTLRRHAGYDHGYYFISTFIEDHLAHHAKVLLG
HHHHHHHHCCCCEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]