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The map label for this gene is livK [C]

Identifier: 91784959

GI number: 91784959

Start: 3969679

End: 3970878

Strand: Reverse

Name: livK [C]

Synonym: Bxe_A0821

Alternate gene names: 91784959

Gene position: 3970878-3969679 (Counterclockwise)

Preceding gene: 91784960

Following gene: 91784958

Centisome position: 81.11

GC content: 61.0

Gene sequence:

>1200_bases
ATGCGAGTCAAGTTTGCTTACGCCGTGTCCATCGCGGCCGCGGTTGCGATGCTGACCGCGTGCGGCAAGAAACAGGATGG
CGAGGCGGGAGCGGGTGCATCCGCGGCTGTGGCCGCAGCGGCACCGGCGAGTGAAGCGACTATCGTGAAGATCGGTCATG
CGGCCCCTTTGACGGGCGGCATAGCGCATCTGGGCAAGGACAACGAAAACGGCGCGCGTCTGGCGGTCGAGGAAATCAAT
ACGCAGGGTTTGACCATCGACGGTCACAAGATCCAGTTGGAGCTGGATGCGCAAGACGACGCAGCGGACCCGAAAACGGG
CACGGCGGTCGCGCAGAAGCTGGTCGACGATCATGTGGTGGCGGTGGTGGGGCACCTGAATTCGGGCGTGTCGATTCCGG
CTTCGAAGATCTATAGCGATGCGAGCATCGTGCAGATCTCGCCGTCGTCGACGAATCCGGCGTACACGCTGCAGGGTTTC
AAGACGACCTACCGGGTCGTCGCCACCGACGCGCAACAAGGTCCGGCGCTCGCCGATTACGCCACGAAGGCGTTGGGCGC
CAAACGCATCGCCATAGTGGACGATGCAACCGCCTACGGTAAGGGGCTCGCGGACGAATTCGCGAAGACCGTACAGGCGA
GCGGAGCGAAGATCGTCGCGCGGGAAGCGACGAACGACAGGGCCACGGATTTCCGGGCCATCCTCACGAAGATCAAGAGT
GTTCAGCCGGACGTGATCATGTTCGGCGGCATGGACGCGACGGGCGGGCCGTTTACCAAACAGGCGGCGGCGCTCGGTAT
CAGGGCAAAAATCCTTGGCGGCGACGGTGTGTGTACCGACAAGGTGGGTGAGCTGGCAGGAACCGCCGTGCAAAACCTGG
TCTGTTCGGAGGCGGGACTCGCGCTTTCGAAAATGGACAAGGGAGCGGACTTCGAAAAGAAGTACGTGGACCGCTTCCAC
ACACCGGTGCAGATTTACGCGCCGTTCACGTATGACGCTGTGTACGTGATTGTCGATGCAATGAAGCGCGCTAATTCGAT
CGAGGCGCCCAAGGTGCTGGCTGCGATGCCGTCGACCGATTACAACGGGGTAATCGGTCACATCGCGTTCGACGACAAGG
GTGATTTGAAAGAGGGCGCCATTACGCTTTACGACTTCAAGGACGGCAAGAAAGCGGTCCTCGACGTCGTAAAGATGTGA

Upstream 100 bases:

>100_bases
ACAATATTGCCCGTCGCAAAATTGGAGTTACAATGCGCGCGTTCTCAAGGCCTCAGGGTCTCCGAACAATAGATTTTGCA
AGGCGCAGGAGACCTATTTA

Downstream 100 bases:

>100_bases
TGACGGGACGTTCAGCCTGACGGCACCGAAACCACCCAACGGTGCCGTTTTTGCATCCGCCCAAGGCGAGCCCGCGACTG
TCATCCAGTCGCCAAGGCGA

Product: branched chain amino acid ABC transporter periplasmic ligand-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MRVKFAYAVSIAAAVAMLTACGKKQDGEAGAGASAAVAAAAPASEATIVKIGHAAPLTGGIAHLGKDNENGARLAVEEIN
TQGLTIDGHKIQLELDAQDDAADPKTGTAVAQKLVDDHVVAVVGHLNSGVSIPASKIYSDASIVQISPSSTNPAYTLQGF
KTTYRVVATDAQQGPALADYATKALGAKRIAIVDDATAYGKGLADEFAKTVQASGAKIVAREATNDRATDFRAILTKIKS
VQPDVIMFGGMDATGGPFTKQAAALGIRAKILGGDGVCTDKVGELAGTAVQNLVCSEAGLALSKMDKGADFEKKYVDRFH
TPVQIYAPFTYDAVYVIVDAMKRANSIEAPKVLAAMPSTDYNGVIGHIAFDDKGDLKEGAITLYDFKDGKKAVLDVVKM

Sequences:

>Translated_399_residues
MRVKFAYAVSIAAAVAMLTACGKKQDGEAGAGASAAVAAAAPASEATIVKIGHAAPLTGGIAHLGKDNENGARLAVEEIN
TQGLTIDGHKIQLELDAQDDAADPKTGTAVAQKLVDDHVVAVVGHLNSGVSIPASKIYSDASIVQISPSSTNPAYTLQGF
KTTYRVVATDAQQGPALADYATKALGAKRIAIVDDATAYGKGLADEFAKTVQASGAKIVAREATNDRATDFRAILTKIKS
VQPDVIMFGGMDATGGPFTKQAAALGIRAKILGGDGVCTDKVGELAGTAVQNLVCSEAGLALSKMDKGADFEKKYVDRFH
TPVQIYAPFTYDAVYVIVDAMKRANSIEAPKVLAAMPSTDYNGVIGHIAFDDKGDLKEGAITLYDFKDGKKAVLDVVKM
>Mature_399_residues
MRVKFAYAVSIAAAVAMLTACGKKQDGEAGAGASAAVAAAAPASEATIVKIGHAAPLTGGIAHLGKDNENGARLAVEEIN
TQGLTIDGHKIQLELDAQDDAADPKTGTAVAQKLVDDHVVAVVGHLNSGVSIPASKIYSDASIVQISPSSTNPAYTLQGF
KTTYRVVATDAQQGPALADYATKALGAKRIAIVDDATAYGKGLADEFAKTVQASGAKIVAREATNDRATDFRAILTKIKS
VQPDVIMFGGMDATGGPFTKQAAALGIRAKILGGDGVCTDKVGELAGTAVQNLVCSEAGLALSKMDKGADFEKKYVDRFH
TPVQIYAPFTYDAVYVIVDAMKRANSIEAPKVLAAMPSTDYNGVIGHIAFDDKGDLKEGAITLYDFKDGKKAVLDVVKM

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=334, Percent_Identity=32.6347305389222, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI48994941, Length=342, Percent_Identity=28.9473684210526, Blast_Score=151, Evalue=8e-38,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41347; Mature: 41347

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVKFAYAVSIAAAVAMLTACGKKQDGEAGAGASAAVAAAAPASEATIVKIGHAAPLTGG
CEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCCEEEEECCCCCCCCC
IAHLGKDNENGARLAVEEINTQGLTIDGHKIQLELDAQDDAADPKTGTAVAQKLVDDHVV
HHHCCCCCCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHE
AVVGHLNSGVSIPASKIYSDASIVQISPSSTNPAYTLQGFKTTYRVVATDAQQGPALADY
EEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHH
ATKALGAKRIAIVDDATAYGKGLADEFAKTVQASGAKIVAREATNDRATDFRAILTKIKS
HHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHC
VQPDVIMFGGMDATGGPFTKQAAALGIRAKILGGDGVCTDKVGELAGTAVQNLVCSEAGL
CCCCEEEEECCCCCCCCCHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
ALSKMDKGADFEKKYVDRFHTPVQIYAPFTYDAVYVIVDAMKRANSIEAPKVLAAMPSTD
CHHHHCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
YNGVIGHIAFDDKGDLKEGAITLYDFKDGKKAVLDVVKM
CCCEEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHCC
>Mature Secondary Structure
MRVKFAYAVSIAAAVAMLTACGKKQDGEAGAGASAAVAAAAPASEATIVKIGHAAPLTGG
CEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEECCCCCCCEEEEECCCCCCCCC
IAHLGKDNENGARLAVEEINTQGLTIDGHKIQLELDAQDDAADPKTGTAVAQKLVDDHVV
HHHCCCCCCCCCEEEEEECCCCCEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHE
AVVGHLNSGVSIPASKIYSDASIVQISPSSTNPAYTLQGFKTTYRVVATDAQQGPALADY
EEEECCCCCCCCCHHHHCCCCEEEEECCCCCCCCEEEECCCEEEEEEEECCCCCCCHHHH
ATKALGAKRIAIVDDATAYGKGLADEFAKTVQASGAKIVAREATNDRATDFRAILTKIKS
HHHHCCCCEEEEEECCHHHCCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHC
VQPDVIMFGGMDATGGPFTKQAAALGIRAKILGGDGVCTDKVGELAGTAVQNLVCSEAGL
CCCCEEEEECCCCCCCCCHHHHHHHCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCC
ALSKMDKGADFEKKYVDRFHTPVQIYAPFTYDAVYVIVDAMKRANSIEAPKVLAAMPSTD
CHHHHCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCC
YNGVIGHIAFDDKGDLKEGAITLYDFKDGKKAVLDVVKM
CCCEEEEEEECCCCCCCCCCEEEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA