Click here to switch to the map view.

The map label for this gene is spoT [H]

Identifier: 91784884

GI number: 91784884

Start: 3885517

End: 3887874

Strand: Reverse

Name: spoT [H]

Synonym: Bxe_A0907

Alternate gene names: 91784884

Gene position: 3887874-3885517 (Counterclockwise)

Preceding gene: 91784885

Following gene: 91784883

Centisome position: 79.41

GC content: 63.53

Gene sequence:

>2358_bases
ATGAGCACGACCCCGCCCACCGCCACGGAAGTGGAACACGACGCCGACTCGCCCTCGTCTGCACGCAAGTACATCGACGC
GGTCCTCGAACAGTCGTTTCGCCATCTGTTCGGCCCCACCGCCACGCCGGAGCAACCGCGCCGGCATGACGTAGTCTCCA
TTGCCAAGCTGACCTCCGCCTTATCCGGCTATCTGCAGCCGGAAGAGATCAAGGAAGTCAAGGCGGCCTTCCATTTCAGC
GACGAAGCCCACCTCGGCCAGTACCGCCAGAGCGGCGAGCCTTACATCACGCATCCGGTTGCCGTCGCGGAAATCTGCGC
CGGCTGGAATCTCGACGCCCAGTCGATCATGGCGGCGCTGCTGCATGACGTGATGGAAGACCAGGGCGTGACCAAGGCCG
AACTCGCCGAGCGTTTCGGCGCGAAGGTCGCGGAACTGGTCGACGGCTTGTCCAAGCTCGACAAGATGGAGTTTCGCAAT
CGCGAGGAAGCGCAGGCGGAAAACTTCCGCAAGATGCTGCTCGCAATGGCGCGCGACGTCCGCGTGATTCTGGTGAAGCT
CGCCGACCGGCTGCACAACATGCGCACGCTGGGTGCCGTGCCGGCCGAAAAACGCCGCCGCGTGGCGCGCGAAACGCTGG
ATATCTATGCGCCGATCGCTCACCGGCTGGGCCTGAACAATACGTATCGCGAGTTGCAGGACCTGAGCTTCGCGAACTTC
AATCCGCACCGGTACGCCACGCTCGAAAAAGCCGTCAAGGCGGCGCGCGGCAACCGGCGCGAAGTGGTCGGCAAGATTCT
CGAGTCGGTGCAGCGCGCGATTGCCGACGCGAAGCTCGACGCCGAAGTCACCGGCCGCGAAAAAACCATCTTCAGCATCT
ACAAGAAGATGCGCGACAAGCAGTTGTCGTTCTCGCAGGTGCTCGACGTGTATGGCTTTCGCGTCGTGGTGGAAAGCGCG
CTGGAGTGCTACACCTGCATCGGCGCGCTGCATGCGTTGTACAAGCCGGTGCCGGGCAAGTTCAAGGATTACATCGCCAT
CCCGAAGGTGAACGGCTACCAGTCGCTGCACACCACGCTGGTCGGCCCGTTCGGCGCGCCGATCGAGTTTCAGGTGCGCA
CGCGCAAGATGCACGAAATCGCCGAAGCGGGCGTGGCCGCGCACTGGCTGTACAAGAACGGCGGCGCCGATCTGAACGAC
GTGCAGAAGCGCGCGCACCAATGGCTGAAGTCGCTGCTCGACATTCAGAGCGAAGCAGGCGATTCGAGCGAATTCCTCGA
GCACGTCAAGATCGATCTGTTCCCGGACGCGGTGTACGTGTTCACGCCGAAGTCGAAGATCATGGCGCTGCCGCGCGGCG
CCACGGCGCTCGACTTCGCGTATTCGATCCACAGCGACCTGGGCAACCAGTGTGTCGCGGTGAAGATAAACAATGAACTG
CTGCCGCTGCGCACCGAGCTGAAGAGCGGCGATATCGTCGAAGTCATCACGGCGCCGTATTCGAAGCCGAATCCAGCGTG
GCTCGGATTCGTGCGCACCGGCAAGGCGCGGTCGGCGATCCGCCATTATCTGAAGACCATGCGCCTGAACGAGTCCGTTC
AGCTTGGTGAGCGTCTGGTCGATCAGGCGCTGAAGGGTTATGGGCTGGCGCTCTCGGACGTCACGCCGGAAGCGTGGGAA
AAGCTCGTGCAGTGGACGGGCAACAAGAACCGCCAGGAAATTTTCGCGGACATCGGCCTTGGCCGGCGTGTCGCGGCGGT
CATGGCCAAGCGCATCGAAGTGCTGATGAGCGGCCGCGACGCCGACGACGACGGCTCGCGCTCCGACAGCAACGCGCCGC
ATGCGCCGCCGGTGGTCATCACCGGTACGGAGGGCATGTCGGTGCAACTGTCCGCATGCTGCCGTCCGATTCCGGGCGAC
GACATCATGGGCTATATCGGCATCGGCCTCGGCATGGCCATTCACACCACGGATTGCCGCGTCGCGCAGCGTATCCACCG
GCGCGATCCGGGCCGCTGGATCGACGTTGCCTGGGCGCCGCAACCGGGGCGTCTGTTCGACGTCGCCGTGAAGGTGCTGG
TCAAGAACACCAAGGGCGTGTTTGCGCGCGTGGCGGCGGATATTACGTCGGCGGATGCCAACATCGTCCACATTGCAATG
GACGAAGATCTGTCGCAGGAATCCACGGTGTTGCGCTTCGTGATTCAGGTCAGCGACCGCGTCCATCTGGCCAACGTGAT
GCGCCGTGTGCGCACGAACCCGGACGTCATGCGCATTGCACGCGAACGGCCGAGCGAGGAAGGCCATCACCGTCACGAGG
GCGGGATGCGGATCGACCGCGAGCGGGCGGACTACTGA

Upstream 100 bases:

>100_bases
GTTTAAGGCGCACTGTTCCGTTTTCGATTGCGCATTTGCCACACCTGTAATTGATCAACGTGCAGACCGGCGTCCAACCC
TAACCGATACGGAGGCGAAC

Downstream 100 bases:

>100_bases
TCGGCTGCGGCGGCCCTGAGTCAGCCTCGGGGCGTTTCTTATCTGTTCGTCCGAATCTGAACGCACCGGCTACGCGCGAG
TCTCAACTGAAACTCAGGGA

Product: (p)ppGpp synthetase I (GTP pyrophosphokinase), SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 785; Mature: 784

Protein sequence:

>785_residues
MSTTPPTATEVEHDADSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSIAKLTSALSGYLQPEEIKEVKAAFHFS
DEAHLGQYRQSGEPYITHPVAVAEICAGWNLDAQSIMAALLHDVMEDQGVTKAELAERFGAKVAELVDGLSKLDKMEFRN
REEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPAEKRRRVARETLDIYAPIAHRLGLNNTYRELQDLSFANF
NPHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKLDAEVTGREKTIFSIYKKMRDKQLSFSQVLDVYGFRVVVESA
LECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLND
VQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNEL
LPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQALKGYGLALSDVTPEAWE
KLVQWTGNKNRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDGSRSDSNAPHAPPVVITGTEGMSVQLSACCRPIPGD
DIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKVLVKNTKGVFARVAADITSADANIVHIAM
DEDLSQESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIARERPSEEGHHRHEGGMRIDRERADY

Sequences:

>Translated_785_residues
MSTTPPTATEVEHDADSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSIAKLTSALSGYLQPEEIKEVKAAFHFS
DEAHLGQYRQSGEPYITHPVAVAEICAGWNLDAQSIMAALLHDVMEDQGVTKAELAERFGAKVAELVDGLSKLDKMEFRN
REEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPAEKRRRVARETLDIYAPIAHRLGLNNTYRELQDLSFANF
NPHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKLDAEVTGREKTIFSIYKKMRDKQLSFSQVLDVYGFRVVVESA
LECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLND
VQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNEL
LPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQALKGYGLALSDVTPEAWE
KLVQWTGNKNRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDGSRSDSNAPHAPPVVITGTEGMSVQLSACCRPIPGD
DIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKVLVKNTKGVFARVAADITSADANIVHIAM
DEDLSQESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIARERPSEEGHHRHEGGMRIDRERADY
>Mature_784_residues
STTPPTATEVEHDADSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSIAKLTSALSGYLQPEEIKEVKAAFHFSD
EAHLGQYRQSGEPYITHPVAVAEICAGWNLDAQSIMAALLHDVMEDQGVTKAELAERFGAKVAELVDGLSKLDKMEFRNR
EEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPAEKRRRVARETLDIYAPIAHRLGLNNTYRELQDLSFANFN
PHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKLDAEVTGREKTIFSIYKKMRDKQLSFSQVLDVYGFRVVVESAL
ECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDV
QKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNELL
PLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQALKGYGLALSDVTPEAWEK
LVQWTGNKNRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDGSRSDSNAPHAPPVVITGTEGMSVQLSACCRPIPGDD
IMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKVLVKNTKGVFARVAADITSADANIVHIAMD
EDLSQESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIARERPSEEGHHRHEGGMRIDRERADY

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=708, Percent_Identity=45.7627118644068, Blast_Score=663, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=31.4787701317716, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 87520; Mature: 87389

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPPTATEVEHDADSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSIAKLTSA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
LSGYLQPEEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWNLDAQSIMAAL
HHCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHHH
LHDVMEDQGVTKAELAERFGAKVAELVDGLSKLDKMEFRNREEAQAENFRKMLLAMARDV
HHHHHHHCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
RVILVKLADRLHNMRTLGAVPAEKRRRVARETLDIYAPIAHRLGLNNTYRELQDLSFANF
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCC
NPHRYATLEKAVKAARGNRREVVGKILESVQRAIADAKLDAEVTGREKTIFSIYKKMRDK
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHC
QLSFSQVLDVYGFRVVVESALECYTCIGALHALYKPVPGKFKDYIAIPKVNGYQSLHTTL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHH
VGPFGAPIEFQVRTRKMHEIAEAGVAAHWLYKNGGADLNDVQKRAHQWLKSLLDIQSEAG
CCCCCCCEEEEHHHHHHHHHHHCCHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCC
DSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNEL
CHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCE
LPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLV
ECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHHH
DQALKGYGLALSDVTPEAWEKLVQWTGNKNRQEIFADIGLGRRVAAVMAKRIEVLMSGRD
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
ADDDGSRSDSNAPHAPPVVITGTEGMSVQLSACCRPIPGDDIMGYIGIGLGMAIHTTDCR
CCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHCCCCCCCCHHHHHHHHHHCCEEECCHHH
VAQRIHRRDPGRWIDVAWAPQPGRLFDVAVKVLVKNTKGVFARVAADITSADANIVHIAM
HHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEE
DEDLSQESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIARERPSEEGHHRHEGGMRIDR
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEECH
ERADY
HCCCC
>Mature Secondary Structure 
STTPPTATEVEHDADSPSSARKYIDAVLEQSFRHLFGPTATPEQPRRHDVVSIAKLTSA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH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HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]