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The map label for this gene is citA [H]

Identifier: 91784834

GI number: 91784834

Start: 3828744

End: 3830045

Strand: Reverse

Name: citA [H]

Synonym: Bxe_A0958

Alternate gene names: 91784834

Gene position: 3830045-3828744 (Counterclockwise)

Preceding gene: 91784837

Following gene: 91784832

Centisome position: 78.23

GC content: 62.6

Gene sequence:

>1302_bases
ATGTCTACTGCGTCACACGCTACTTCCTCATCGGAAGAATCCAAGGTCAAGACAGTATTCCGCGTGGTCAGCGGCAACTT
TCTTGAGATGTACGACTTCATGGTCTATGGCTATTACGCTTCGGCGATCGCCAAAACCTATTTCCCGAGCGGCAACGAGT
TTGCCTCGCTGATGCTGTCGCTGTCGGTGTTCGGCGCGGGCTTCCTGATGCGGCCGCTCGGTGCGATCGTGCTCGGCGCC
TACATCGACCATCACGGCCGGCGCAAAGGCCTGATCCTCACGCTCGGCCTGATGGCGCTCGGCACGCTTACGGTGGCGGC
AATTCCGGGTTACGCGACGATCGGCGTACTCGCGCCGGCGCTCGTGCTGTTCGGCCGGCTGTTGCAGGGCTTCTCGGCCG
GGGTCGAACTGGGCGGCGTCTCGGTGTATCTGTCGGAGATCGCCACCAAGGGAAACAAGGGCTTCTATTGCGCGTGGCAG
TCGGGCAGCCAGCAGGTTGCCGTGGTGTTCGCCGCGCTGATCGGCGTGTTCCTGAACAAGCTGCTGCCCGCCGACCAGAT
GGGCGCCTGGGGCTGGCGCGTGCCGTTCCTGATCGGCTGCCTGATCGTGCCGTTCCTGTTCCTGATCCGCCGTTCGCTGC
AGGAAACCGAGGAATTCAAGGCGCGCAAGCACCATCCGAAGATGGGCGAGATCATGAAGACGATGGCCGCGAACTGGGGC
ATCGTGCTGGGCGGCATGGGCATGGTGATCATGACCACGGTGTCGTTCTATATGATCACGGCCTACACGCCGACCTTCGG
CAAGGAAGTGCTGAAACTGTCGTCGATCGATACGCTCGTCGTCACGGTCTGTATCGGTCTGTCGAATCTGATCTGGCTGC
CGCTCGCGGGTGCGTTGTCGGACCGTGTCGGCCGCCGTCCGGTGCTGCTGGCCTTTACGATTCTGACCATCATTACCGCG
TATCCGGCGCTGCAATGGCTGGTGGCGGATCCGTCGTTTGCGCGTCTGCTGGCAGTCGAATTGTGGCTGTCGTTCCTGTA
CGGCAGCTATAACGGCGCGATGGTCGTCGCGCTGACCGAGGTCATGCCGGTCGAAGTGCGGACCGCCGGCTTCTCGCTCG
CCTACAGCCTGGCTACGACGATCGGCGGCTTCACGCCGGCTATCTCCACGTTGCTGATTCATACCACCGGCAACAAGGCG
GCGCCGGGTCTGTTCCTGGGCGTGGCGGCGATTTGCGGGTTGATTGCGACGCTGGTGCTGTACCGCACGCCTGAATCGCG
CGACCAGTACCGGACGGCCTGA

Upstream 100 bases:

>100_bases
TCTATCCGTCCGAGGCATCCGCCCGCCGAACCCAGTGGATCGTTTCAACTGCCTGCGCGTCACTGTTTCTTTCGTGGCAG
CAGGTCCATCAGAGAATGTC

Downstream 100 bases:

>100_bases
CTTCAGCCGGTCACCGGCCTGCCGGTCAAGTAAAAAGCCCGCGCGTTCTCACGAACGCGGAGGCTTTGTTTTTGGCGCGG
GGTCCATGCAGCCATTCGCT

Product: major facilitator superfamily citrate/H(+) symporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSTASHATSSSEESKVKTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNEFASLMLSLSVFGAGFLMRPLGAIVLGA
YIDHHGRRKGLILTLGLMALGTLTVAAIPGYATIGVLAPALVLFGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYCAWQ
SGSQQVAVVFAALIGVFLNKLLPADQMGAWGWRVPFLIGCLIVPFLFLIRRSLQETEEFKARKHHPKMGEIMKTMAANWG
IVLGGMGMVIMTTVSFYMITAYTPTFGKEVLKLSSIDTLVVTVCIGLSNLIWLPLAGALSDRVGRRPVLLAFTILTIITA
YPALQWLVADPSFARLLAVELWLSFLYGSYNGAMVVALTEVMPVEVRTAGFSLAYSLATTIGGFTPAISTLLIHTTGNKA
APGLFLGVAAICGLIATLVLYRTPESRDQYRTA

Sequences:

>Translated_433_residues
MSTASHATSSSEESKVKTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNEFASLMLSLSVFGAGFLMRPLGAIVLGA
YIDHHGRRKGLILTLGLMALGTLTVAAIPGYATIGVLAPALVLFGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYCAWQ
SGSQQVAVVFAALIGVFLNKLLPADQMGAWGWRVPFLIGCLIVPFLFLIRRSLQETEEFKARKHHPKMGEIMKTMAANWG
IVLGGMGMVIMTTVSFYMITAYTPTFGKEVLKLSSIDTLVVTVCIGLSNLIWLPLAGALSDRVGRRPVLLAFTILTIITA
YPALQWLVADPSFARLLAVELWLSFLYGSYNGAMVVALTEVMPVEVRTAGFSLAYSLATTIGGFTPAISTLLIHTTGNKA
APGLFLGVAAICGLIATLVLYRTPESRDQYRTA
>Mature_432_residues
STASHATSSSEESKVKTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNEFASLMLSLSVFGAGFLMRPLGAIVLGAY
IDHHGRRKGLILTLGLMALGTLTVAAIPGYATIGVLAPALVLFGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYCAWQS
GSQQVAVVFAALIGVFLNKLLPADQMGAWGWRVPFLIGCLIVPFLFLIRRSLQETEEFKARKHHPKMGEIMKTMAANWGI
VLGGMGMVIMTTVSFYMITAYTPTFGKEVLKLSSIDTLVVTVCIGLSNLIWLPLAGALSDRVGRRPVLLAFTILTIITAY
PALQWLVADPSFARLLAVELWLSFLYGSYNGAMVVALTEVMPVEVRTAGFSLAYSLATTIGGFTPAISTLLIHTTGNKAA
PGLFLGVAAICGLIATLVLYRTPESRDQYRTA

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=424, Percent_Identity=29.2452830188679, Blast_Score=207, Evalue=7e-55,
Organism=Escherichia coli, GI1788942, Length=423, Percent_Identity=33.096926713948, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1788292, Length=398, Percent_Identity=27.1356783919598, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789941, Length=426, Percent_Identity=29.81220657277, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87082231, Length=203, Percent_Identity=27.0935960591133, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 46559; Mature: 46427

Theoretical pI: Translated: 9.54; Mature: 9.54

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCCCC
>Mature Secondary Structure 
STASHATSSSEESKVKTVFRVVSGNFLEMYDFMVYGYYASAIAKTYFPSGNEFASLMLS
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LSVFGAGFLMRPLGAIVLGAYIDHHGRRKGLILTLGLMALGTLTVAAIPGYATIGVLAPA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LVLFGRLLQGFSAGVELGGVSVYLSEIATKGNKGFYCAWQSGSQQVAVVFAALIGVFLNK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHH
LLPADQMGAWGWRVPFLIGCLIVPFLFLIRRSLQETEEFKARKHHPKMGEIMKTMAANWG
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
IVLGGMGMVIMTTVSFYMITAYTPTFGKEVLKLSSIDTLVVTVCIGLSNLIWLPLAGALS
EEECCCHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRVGRRPVLLAFTILTIITAYPALQWLVADPSFARLLAVELWLSFLYGSYNGAMVVALTE
HHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
VMPVEVRTAGFSLAYSLATTIGGFTPAISTLLIHTTGNKAAPGLFLGVAAICGLIATLVL
CCCEEEECCCHHHHHHHHHHHCCHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHHH
YRTPESRDQYRTA
HCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]