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The map label for this gene is pcpB [H]

Identifier: 91784830

GI number: 91784830

Start: 3823312

End: 3825036

Strand: Reverse

Name: pcpB [H]

Synonym: Bxe_A0962

Alternate gene names: 91784830

Gene position: 3825036-3823312 (Counterclockwise)

Preceding gene: 91784831

Following gene: 91784825

Centisome position: 78.13

GC content: 67.54

Gene sequence:

>1725_bases
ATGGACGCTGCCGCAAACGATGCGTCGCCGGTGCTGATCGTCGGGGCGGGTCCCACCGGGCTCGCCGCAGCGATGAGTCT
CGCGCGTGCCCACATCCCCGTGCGTCTGATCGACAAGGCCATGCAGCCGAACCCGTATTCGCGGGCCATCGGCATCCAGG
CGCGCACGCTCGAGTTGCTCGAGCAGCATCGGCTGGTCGAGCCGTTTCTCGAGCTCGGCCATCGCGCGCGCATCGCCAAT
CTGTTTTCGAACGGCATGCGTCTCGCGCAGCTGGATTTCGATCCGTTGCACACGCGCTATCCGTATCTGCTGTTTCTCGA
TCAGTCCGTGACCGAGCGCCTGCTGACCGAGCATCTCGCGACGTTCGGCGTGACGATCGAACGGGGCGTCGAGCTCACGA
TGTTCGCGCAAGGCGCCGCCAGCATTCAGGCGACGCTGCGCCGCGCGGACGGCCACACGGAGACCATGCGTCCGTCGTAC
ATGATCGCCGCCGACGGCGCACATAGCGCGATCCGTCACCGGCTCGGTTTGAACTTCGAGGGCAAGACCTTCGAGCAGAC
TTTCCTGCTCGCCGACCTGCACGCAGAAACCGATTGGCCCGAAGACGAGTTCCATATTTTCGCGTCGGGCGAAGGACTGG
TCGCGCTGTTTCCGATGGGCCACGGCAGGCATCGTCTGATCGCGGATCATGCCGTCGAGCCTGCCGGCGTCGCGCCGGCC
GAAACGCCGGCGGTGCTGGGCGAGCCACCGCTGAACAGGGTGCCGCCGCCTTCGCTGGAAGAATGCAAGGCGCTGATCGC
GCGGCGCGTGCGCGAGCGGGTCGACGTGTCGGATCTCGCGTGGTCCTCGTATTTTCATGTGAATAGCCGCATGGTGGACC
GGCTGCGCGTCGGCCGCATCTTCCTTGCGGGCGACGCCGCGCATGTGCACAGCCCGGCCGGCGCGCAGGGCATGAACACA
GGCATTCAGGAAGCGTTCAATCTCGGCTGGAAGCTCGCGCGCGTGCTGAAAGGCGCGGCGCCGGATCGTCTGCTCGACAC
CTATCACCTCGAACGCCATCCTATCGAACGTGACGTGCTGCGTCAGACGGGTTTCATCACGCAGATGGCGGAGGCCGATC
ACGGGCCGCTGAAGCTGCTGCGCGAACGGGTGATGCCGGTGCTCGCCGCGCTGGGTCCGCTGCGCGACGCCGCGCGCCTG
GCCATCAGCGAGTTGTCGATCCAGTACCGGCGCAGTCCGTTGACGCTCGAACGGGTGCTCGACGGCGGACCGCGCGCCGG
CGAGCGCGCACCGGACGCGCTGGTGCATGTGGTCGACGGACCGCTCGGCCGTGCGCCCGGCGTCGGCTGCGTGTTCGATC
TGCACGATCCGGCGTTCTTCTCGCTGTTCCTGCTGGTGCCGCCGCTGCCCGTCGACGAAAAACCGCTGGATCCCGCGGCG
AAGCATGCGCCGCCGCCGGACGCGGAACTGGAGCGCGTGGCAGCGGAAGTCGAGAGATTGCTGCCGGGCGCGGTGCGCAT
CTGGCGCATTACGGATACCACCGGCGACGGTGGACCGGCGCTTGCCGAATCGTATGGCCGCACGCGGCCGTCGTTCTATC
TCGTGCGACCGGACGGCTACATCAGCGCGCGCGGGCGCACCGGCTCCGACCTGCACGGCCTGTTGCGGCATTGCGAAGCG
TGGTTCGCGGTGAGCGGAGAGATACCGGAGCAGGCGGCCTTGTGA

Upstream 100 bases:

>100_bases
GATTGATGCAACGCAACCGCCCGGCCGGTTGTCGGCGGCGCTCCCAAGGTGACTTCCCGCGGGCGTACACTAGAACGCAT
TGTTGTCTCCGGAGTTCCGC

Downstream 100 bases:

>100_bases
CGCGGGCCGCCTGAGTCGCGCGGGTGGTGAATCCGGCGAGCGCGGCCCGGCTTACACCGTTGCCGGTGCAGCGGGCGTCA
GTTGCTCGCGATGTTTGATG

Product: putative monooxygenase, FAD-binding

Products: NA

Alternate protein names: Pentachlorophenol hydroxylase [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MDAAANDASPVLIVGAGPTGLAAAMSLARAHIPVRLIDKAMQPNPYSRAIGIQARTLELLEQHRLVEPFLELGHRARIAN
LFSNGMRLAQLDFDPLHTRYPYLLFLDQSVTERLLTEHLATFGVTIERGVELTMFAQGAASIQATLRRADGHTETMRPSY
MIAADGAHSAIRHRLGLNFEGKTFEQTFLLADLHAETDWPEDEFHIFASGEGLVALFPMGHGRHRLIADHAVEPAGVAPA
ETPAVLGEPPLNRVPPPSLEECKALIARRVRERVDVSDLAWSSYFHVNSRMVDRLRVGRIFLAGDAAHVHSPAGAQGMNT
GIQEAFNLGWKLARVLKGAAPDRLLDTYHLERHPIERDVLRQTGFITQMAEADHGPLKLLRERVMPVLAALGPLRDAARL
AISELSIQYRRSPLTLERVLDGGPRAGERAPDALVHVVDGPLGRAPGVGCVFDLHDPAFFSLFLLVPPLPVDEKPLDPAA
KHAPPPDAELERVAAEVERLLPGAVRIWRITDTTGDGGPALAESYGRTRPSFYLVRPDGYISARGRTGSDLHGLLRHCEA
WFAVSGEIPEQAAL

Sequences:

>Translated_574_residues
MDAAANDASPVLIVGAGPTGLAAAMSLARAHIPVRLIDKAMQPNPYSRAIGIQARTLELLEQHRLVEPFLELGHRARIAN
LFSNGMRLAQLDFDPLHTRYPYLLFLDQSVTERLLTEHLATFGVTIERGVELTMFAQGAASIQATLRRADGHTETMRPSY
MIAADGAHSAIRHRLGLNFEGKTFEQTFLLADLHAETDWPEDEFHIFASGEGLVALFPMGHGRHRLIADHAVEPAGVAPA
ETPAVLGEPPLNRVPPPSLEECKALIARRVRERVDVSDLAWSSYFHVNSRMVDRLRVGRIFLAGDAAHVHSPAGAQGMNT
GIQEAFNLGWKLARVLKGAAPDRLLDTYHLERHPIERDVLRQTGFITQMAEADHGPLKLLRERVMPVLAALGPLRDAARL
AISELSIQYRRSPLTLERVLDGGPRAGERAPDALVHVVDGPLGRAPGVGCVFDLHDPAFFSLFLLVPPLPVDEKPLDPAA
KHAPPPDAELERVAAEVERLLPGAVRIWRITDTTGDGGPALAESYGRTRPSFYLVRPDGYISARGRTGSDLHGLLRHCEA
WFAVSGEIPEQAAL
>Mature_574_residues
MDAAANDASPVLIVGAGPTGLAAAMSLARAHIPVRLIDKAMQPNPYSRAIGIQARTLELLEQHRLVEPFLELGHRARIAN
LFSNGMRLAQLDFDPLHTRYPYLLFLDQSVTERLLTEHLATFGVTIERGVELTMFAQGAASIQATLRRADGHTETMRPSY
MIAADGAHSAIRHRLGLNFEGKTFEQTFLLADLHAETDWPEDEFHIFASGEGLVALFPMGHGRHRLIADHAVEPAGVAPA
ETPAVLGEPPLNRVPPPSLEECKALIARRVRERVDVSDLAWSSYFHVNSRMVDRLRVGRIFLAGDAAHVHSPAGAQGMNT
GIQEAFNLGWKLARVLKGAAPDRLLDTYHLERHPIERDVLRQTGFITQMAEADHGPLKLLRERVMPVLAALGPLRDAARL
AISELSIQYRRSPLTLERVLDGGPRAGERAPDALVHVVDGPLGRAPGVGCVFDLHDPAFFSLFLLVPPLPVDEKPLDPAA
KHAPPPDAELERVAAEVERLLPGAVRIWRITDTTGDGGPALAESYGRTRPSFYLVRPDGYISARGRTGSDLHGLLRHCEA
WFAVSGEIPEQAAL

Specific function: Dechlorination of pentachlorophenol to tetrachlorohydroquinone. Removes also hydrogen and nitro, amino, and cyano groups from benzene ring at the para position in relation to the hydroxyl of phenol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pheA/tfdB FAD monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786543, Length=359, Percent_Identity=30.3621169916435, Blast_Score=131, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: =1.14.13.50 [H]

Molecular weight: Translated: 62761; Mature: 62761

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAAANDASPVLIVGAGPTGLAAAMSLARAHIPVRLIDKAMQPNPYSRAIGIQARTLELL
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHEECCHHHHHHHH
EQHRLVEPFLELGHRARIANLFSNGMRLAQLDFDPLHTRYPYLLFLDQSVTERLLTEHLA
HHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHH
TFGVTIERGVELTMFAQGAASIQATLRRADGHTETMRPSYMIAADGAHSAIRHRLGLNFE
HHCCCHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCC
GKTFEQTFLLADLHAETDWPEDEFHIFASGEGLVALFPMGHGRHRLIADHAVEPAGVAPA
CCCHHHEEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEEEHHHCCCCCCCCCC
ETPAVLGEPPLNRVPPPSLEECKALIARRVRERVDVSDLAWSSYFHVNSRMVDRLRVGRI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHEEHHHHHHHHHHHCEE
FLAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLARVLKGAAPDRLLDTYHLERHPIERDVL
EEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
RQTGFITQMAEADHGPLKLLRERVMPVLAALGPLRDAARLAISELSIQYRRSPLTLERVL
HHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
DGGPRAGERAPDALVHVVDGPLGRAPGVGCVFDLHDPAFFSLFLLVPPLPVDEKPLDPAA
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCHH
KHAPPPDAELERVAAEVERLLPGAVRIWRITDTTGDGGPALAESYGRTRPSFYLVRPDGY
HCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCCCE
ISARGRTGSDLHGLLRHCEAWFAVSGEIPEQAAL
EEECCCCCHHHHHHHHHHHHHEEECCCCCHHCCC
>Mature Secondary Structure
MDAAANDASPVLIVGAGPTGLAAAMSLARAHIPVRLIDKAMQPNPYSRAIGIQARTLELL
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHEECCHHHHHHHH
EQHRLVEPFLELGHRARIANLFSNGMRLAQLDFDPLHTRYPYLLFLDQSVTERLLTEHLA
HHHHHHHHHHHHCCHHHHHHHHHCCCEEEEECCCCCCCCCCEEEEECHHHHHHHHHHHHH
TFGVTIERGVELTMFAQGAASIQATLRRADGHTETMRPSYMIAADGAHSAIRHRLGLNFE
HHCCCHHCCCEEEEEECCHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHCCCCC
GKTFEQTFLLADLHAETDWPEDEFHIFASGEGLVALFPMGHGRHRLIADHAVEPAGVAPA
CCCHHHEEEEEECCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEEEHHHCCCCCCCCCC
ETPAVLGEPPLNRVPPPSLEECKALIARRVRERVDVSDLAWSSYFHVNSRMVDRLRVGRI
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHEEHHHHHHHHHHHCEE
FLAGDAAHVHSPAGAQGMNTGIQEAFNLGWKLARVLKGAAPDRLLDTYHLERHPIERDVL
EEECCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHH
RQTGFITQMAEADHGPLKLLRERVMPVLAALGPLRDAARLAISELSIQYRRSPLTLERVL
HHHCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
DGGPRAGERAPDALVHVVDGPLGRAPGVGCVFDLHDPAFFSLFLLVPPLPVDEKPLDPAA
CCCCCCCCCCCCCEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCCCCCCCHH
KHAPPPDAELERVAAEVERLLPGAVRIWRITDTTGDGGPALAESYGRTRPSFYLVRPDGY
HCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHCCCCCCEEEECCCCE
ISARGRTGSDLHGLLRHCEAWFAVSGEIPEQAAL
EEECCCCCHHHHHHHHHHHHHEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7678243; 12169590 [H]